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Characterization and optimization for beverage manufacture using Korean red ginseng extract (기호성 개선 인삼농축액 제조를 위한 추출 조건 설정)

  • Choi, Ji-Won;Oh, Mi-Jin;Ha, Sang Keun;Park, Yongkon;Park, Ho-Young
    • Food Science and Preservation
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    • v.23 no.3
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    • pp.319-325
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    • 2016
  • This study was designed to develop a manufacturing process for ginseng concentrate with reduced unpleasant aroma and bitter taste. Two types of ginseng, white and red, were extracted under six different conditions (the 1st to the 6th step) of temperature ($65{\sim}95^{\circ}C$) and ethanol concentration (0~70%). Six extracts of each ginseng were evaluated by a sensory test, and assayed for crude saponin, ginsenosides, and acidic polysaccharides. The content of crude saponin in the extracts decreased with extraction time. There was no significant difference in the crude saponin content between white and red ginseng extracts. The yield of red ginseng extract was higher (45%) than that of white ginseng. No significant difference was observed in the acidic polysaccharide content between red and white ginseng extracts. $Rg_3$, a specific ginsenoside in red ginseng, was detected in the 1st to 6th extracts of red ginseng. Bitterness, astringency, and sourness of ginseng extracts decreased as the extraction steps proceeded. The composite of the 1st, 2nd, and 6th step extracts decreased bitterness and astringency, and the highest overall acceptance. Compared with commercial beverages, the composition of the three extracts is the desirable method to decrease the bitter and astringent tastes, and the overall unpleasant flavor of ginseng.

Comparison of Antimicrobial and Antioxidant Activities of Prunus mume Fruit in Different Cultivars (매실 품종별 항균 및 항산화성 비교)

  • Seo, Kyoung-Sun;Huh, Chang-Ki;Kim, Yong-Doo
    • Food Science and Preservation
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    • v.15 no.2
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    • pp.288-292
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    • 2008
  • This study examined the general components and biological activities of fruit from different cultivars of Prunus mume. The average moisture content of fruit ranged from 89.94-90.62%. The levels of crude protein, crude fat and crude fiber were not significantly different in the different cultivars. In an antimicrobial activity test, methanol extracts showed the highest activity and extracts from the Chunmae and Baekgaha cultivars had higherantimicrobial activity than other cultivars. The extract of fruit. However, there was no antimicrobial activity against the lactic acid- producing bacteria and yeasts, even at a concentration of 1.5 mg/mL or higher. Antimicrobial substances in methanol extracts of the fruit maintained their activity after heating at $100^{\circ}C$ for 30 minutes and were also unaffected by changes in pH. The antioxidant activities of extracts isolated with different solvents were: methanol > ethylacetate > water> ether> hexane. Antioxidant activity was not significantly different at different harvest times. The antioxidant index of the methanol extract was also the highest in electron donating activity.

Effects of Dietary Inclusion of Sea Tangle Laminaria japonica on Growth, Body Composition and Plasma Chemistry of Black Porgy Acanthopagrus schlegeli (사료내 다시마(Laminaria japonica) 첨가에 따른 감성돔(Acanthopagrus schlegeli)의 성장, 체조성 및 혈액성상에 미치는 영향)

  • Cho, Sung Hwoan;Min, Byeong-Hee;Lee, Jin Young;Lee, Sang Yoon;Kim, Ha-Na;An, Shinhong;Na, Kyoung-Suk;Kim, Dong Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.46 no.5
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    • pp.559-564
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    • 2013
  • This study was performed to determine effects of dietary inclusion of various concentrations and sources of sea tangle Laminaria japonica on growth, body composition and plasma chemistry of juvenile black porgy Acanthopagrus schlegeli. Juvenile fish (N=810; average mass=8.9 g) were distributed among twenty-seven 200 L flow-through tanks (30 fish per tank). Nine experimental diets were prepared: control (Con) without additives; sea tangle powder at 0.1%, 0.5%, 1%, 2%, 3% and 5%, (P-0.1, P-0.5, P-1, P-3 and P-5 diets, respectively); and fresh sea tangle (F-1); and 1% sea tangle extract (E-1). Each powdered additive was substituted for an eqaul amount of wheat flour, and the extract was substituted for an equal amount of water in the respective experiemtnal diets. Each diet was fed to triplicate groups of fish. Weight gain and feed conversion ratio were not affected by the experimental diets. Feed consumption by fish fed the P-1 diet was higher than that of fish fed the P-3 and P-5 diets. Moisture, crude protein, crude lipid, whole-body ash content (excluding the liver), liver moisture, and crude protein and lipid contents of fish were not affected by the experimental diets. None of the fish plasma criteria were affected by the experimental diets, with the exception of plasma glucose. In conclusion, dietary inclusion of various concentrations and sources of sea tangle did not significantly affect the growth, feed efficiency, body composition or plasma chemistry of juvenile black porgy.

Alcoholic Fermentation of Bokbunja (Rubus coreanus Miq.) Wine (복분자 발효주의 양조특성)

  • Choi, Han-Seok;Kim, Myung-Kon;Park, Hyo-Suk;Kim, Yong-Suk;Shin, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.38 no.4
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    • pp.543-547
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    • 2006
  • In order to improve wine quality, the selection of yeast strain and of additives in the manufacture of Bokbunja (Rubus coreanus Miq.) wine was investigated. The chemical composition of the edible portions of Bokbunja fruits was 86.5% moisture, 0.2% crude protein, 0.9% crude fat, 6.6% crude fiber, 0.5% ash and $10^{\circ}Brix$ sugar, and was 2.99% fructose, 2.53% glucose and 0.07% sucrose in fruit extract. The predominant organic acids in the fruit were citric acid (14.57 mg/mL) and malic acid (2.24 mg/mL) with smaller amounts of shikimic, pyroglutamic and oxalic acid. During fermentation, citric and malic acid levels decreased, while formic and acetic acid were released. Saccharomyces cerevisiae KCCM 12224 (Sc-24) was more favorable for alcoholic fermentation of Bokbunja and the addition of 200 ppm of potassium metabisulphite to must was more efficient than other $SO_2$ sources with a higher overall acceptability score. Sc-24 increased alcohol production from 9.8 to 14.8% in a sugar concentration dependent manner $(18-28^{\circ}Brix)$. The color value of early stage Bokbunja must was improved by supplementing with Japanese apricot extract, but this did not influence the color value of Bokbunja wine after primary fermentation. The astringent taste of Bokbunja wine was reduced by removing the seed from the fruit. Sugar solution (50%, w/v) was used instead of sugar power to prevent the possibility of undissolved sugar due to insufficient mixing. This substitution did not influence sensory evaluation.

Monensin Enriched Urea Molasses Mineral Block on Feed Intake, Nutrient Digestibility and Blood Glucose in Cattle Fed on Wheat Straw Based Diet

  • De, Debasis;Singh, G.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.11
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    • pp.1579-1584
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    • 2002
  • Twelve adult male crossbred (Sahiwal${\times}$Holstein Friesian) cattle were distributed into four groups of three each on body weight basis. Animals were given wheat straw as a basal diet. The animals of group I and II were supplemented with concentrate mixture and animals of group III and IV were supplemented with cold processed urea molasses mineral block (UMMB). Thirty mg monensin/day/animal was supplemented to the animals of group II and 35 ppm monensin were incorporated in the UMMB supplemented to the animals of group IV. Vit.A and D mixture was given to all the animals once a week. Dry matter (DM) intake (kg/d) through wheat straw was 19.0 percent higher in the UMMB (without monensin) supplemented group (group III) than those of the concentrate mixture (without monensin) supplemented group i.e. group I. Total DM intake (kg/d) was lower in the monensin supplemented groups than those of non-supplemented groups though differences were not statistically significant. Digestible dry matter, organic matter (OM), crude protein (CP) and total digestible nutrients (TDN) intake were similar in all the groups. Average block consumption for 45 d period in the group III (0.95 kg/d) and group IV (0.84 kg/d) did not differ significantly. DM digestibility (%) was significantly (p<0.01) higher in the group II (58.9) as compared to the group I (52.7) and group III (54.0) but similar to the group IV (57.2). OM digestibility was also significantly (p<0.05) higher in the group II (63.2) as compared to that of the group I (54.9) but similar to the group III (57.8) and IV (59.2). Ether extract (EE) digestibility was significantly (p<0.01) higher in the group I (76.9) and II (80.3) as compared to the group III (59.87) and IV (55.77). Nitrogen free extract (NFE) digestibility was significantly (p<0.05) higher in the group II (62.38) as compared to that of the other groups. Crude protein (CP) and crude fibre (CF) digestibilities were not affected either due to UMMB or monensin. Nitrogen balance did also not differ significantly among the groups. However, Ca and P balance (g/d) in the group III (3.1, 1.3) and IV (3.0, 1.4) were significantly (p<0.01) higher than those of the group I (0.6, 0.2) and II (0.4, 0.3). Blood glucose (mg/100ml) was significantly (p<0.01) higher in the group II (65.2) and IV (65.2) as compared to the group I (55.2) and group III (53.9). Plasma urea-N level (mg/100 ml) in the group III (19.0) and IV (17.8) were significantly (p<0.01) higher than that of the group I (10.5) and II (12.3). So, monensin supplementation either with cold process UMMB or concentrate mixture did not show any additional effect on feed intake and digestibility but increases blood glucose level in adult cattle.

Effect of Replacement of Groundnut Cake with Urea-treated Neem (Azadirachta indica A. juss) Seed Kernel Cake on Nutrient Utilisation in Lambs

  • Musalia, L.M.;Anandan, S.;Sastry, V.R.B.;Katiyar, R.C.;Agrawal, D.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.9
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    • pp.1273-1277
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    • 2002
  • The effect of urea treatment on chemical composition of neem seed kernel cake (NSKC) was investigated by soaking the cake in 2.1% urea solution (1.2 l $kg^{-1}$ NSKC) for five days. The effect on utilisation of nutrients by replacing groundnut cake (GNC) (30%) with urea-treated neem seed kernel cake (UTNSKC) (33%) in a concentrate mixture fed to meet 70% of the protein requirements of lambs (8 males and 8 females), was monitored in a digestibility study. Following urea treatment of NSKC only 9.5% of urea was hydrolysed and the crude protein content of the cake was increased by 6.65%. The tannin content in depulped neem seeds was 37% catechin equivalent. Whereas feeding UTNSKC had no effect on intake of dry matter (72.5 vs 66.3 g/kg $BW^{0.75}day^{-1}$) and digestibility of crude fibre (41.3 vs 43.4%), the cake depressed (p<0.01) the percent digestibility of dry matter (63.7 vs 70.2), crude protein (63.2 vs 70.2), nitrogen free extract (73.8 vs 80.5) and gross energy (64.3 vs 69.1). Digestibility of ether extract (75.8 vs 70.9%) was higher (p<0.05) in animals offered UTNSKC. The nutritive value of the composite ration consumed by lambs offered UTNSKC was lower (p<0.01) in terms of total digestible nutrients (64.7 vs 70.2%) and digestible energy (2.8 vs 3.0 Kcal/g DM). Intake of digestible energy (199.8 vs 194.1 Kcal/kg $BW^{0.75}day^{-1}$) and retention of nitrogen (7.53 vs 8.23 g $day^{-1}$) and calcium (2.12 vs 1.84 g $day^{-1}$) were comparable on the 2 rations. Animals fed UTNSKC retained less (p<0.01) phosphorus (0.37 vs 1.05 g $day^{-1}$). The results indicate that urea treatment increased the protein level of NSKC whereas feeding the treated cake as a replacement of GNC, lowered the digestibility of nutrients and retention of phosphorus.

Effects of Environmentally Friendly Agents on Growth Performance, Nutrient Digestibility, Nutrient Excretion and Carcass Characteristics in Growing-Finishing Pigs

  • Min, T.S.;Kim, J.D.;Hyun, Y.;Sohn, K.S.;Heo, K.N.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.4
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    • pp.540-547
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    • 2001
  • An experiment was conducted to investigate the effects of environmentally friendly agents (yucca extract, mineral feed additive, acidifier, nonspecific immunostimulating anionic alkali solution) on the growth performance, nutrient digestibility, nutrient excretion and carcass characteristics in growing-finishing pigs. A total of 125 pigs were allotted into 5 treatments with 5 replications, 5 pigs per pen in a completely randomized block design. Dietary treatments consisted of 1) control: basal diet, 2) YE: basal diet+120 mg/kg of yucca extract, 3) MFA: basal diet+50 mg/kg of mineral feed additive, 4) NIS: basal diet+3% of NIS (nonspecific immunostimulating anionic alkali solution), and 5) Acidifier: basal diet+0.5% of acidifier (organic acid complex). During the growing phase of the feeding trial, There were no significant differences in average daily feed intake (ADFI) and feed conversion ratio (F/G) among treatments, but the Acidifier group showed higher average daily gain (ADG) than the MFA group (p<0.05). During the finishing phase, there were no significant differences in growth performance among treatments, however YE supplementation increased ADG and F/G by 3.1 and 6.15%, respectively, when compared to control group. Proximate nutrients (dry matter, DM; crude protein, CP; crude ash, CA; crude fat, CF; gross energy, GE; calcium, Ca and phosphorus, P) digestibility did not show any significant differences among the treatments. And there were no significant differences in DM, N and P excretion as well. The supplementation of environmentally friendly agents tended to increase carcass weight compared to control group. Pigs fed MFA showed significantly heavier (p<0.05) carcass weight than those fed control or Acidifier diets. Addition of these agents except for NIS to diet for growing-finishing pigs caused a similar feed cost/kg weight gain than control group. Therefore, present study suggested that the optimum environmentally friendly agent would be yucca extracts, considering the effects on growth performance, nutrient digestibility, nutrient excretion, carcass characteristics and feed cost in growing-finishing pigs.

Analysis of Nutritional Compositions and Antioxidative Activities of Korean Commercial Blueberry and Raspberry (국내 시판 블루베리와 라즈베리의 영양성분 분석 및 항산화 활성)

  • Jeong, Chang-Ho;Choi, Sung-Gil;Heo, Ho-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.11
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    • pp.1375-1381
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    • 2008
  • The nutritional compositions and antioxidative activities of Korean commercial blueberry and raspberry were investigated. The proximate compositions were 10.47% and 22.67% in moisture, 2.66% and 2.64% in crude protein, 2.04% and 1.67% in crude fat, 81.36% and 70.19% in nitrogen free extracts, 1.48% and 0.85% in crude fiber, and 1.99% and 1.98% in ash of blueberry and raspberry, respectively. Total phenolics content were higher in blueberry (9.028 mg/g) than in raspberry (5.340 mg/g). Major elements of blueberry and raspberry were Ca (451.34 and 97.48 mg/100 g), K (355.40 and 215.20 mg/100 g), P (321.10 and 294.04 mg/100 g), and Na (137.58 and 137.67 mg/100 g). The total amino acid contents of blueberry and raspberry were 2,011.44 mg /100 g and 2,098.82 mg/100 g, respectively. Amino acid were mainly composed of glutamic acid, aspartic acid and leucine. The DPPH and ABTS radical scavenging activities of the 80% methanol extract from blueberry and raspberry were 88.67% and 62.77%, 76.34% and 30.53% at a concentration of 5 mg/mL. The 80% methanol extract from blueberry and raspberry showed considerable antioxidative activity against reducing power in dose-dependent manner. Antioxidative activities using $\beta$-carotene-linoleate and FTC method were twice higher in blueberry than raspberry.

Production of Biosurfactant by Pseudomonas sp. SW1 for Microbial Remediation of Oil Pollution (유류오염방제를 위한 Pseudomonas sp. SW1로부터 생물계면활성제의 생산)

  • Son, Hong-Joo;Suk, Wan-Su;Lee, Geon;Lee, Sang-Joon
    • Korean Journal of Microbiology
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    • v.33 no.3
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    • pp.193-198
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    • 1997
  • Microorganisms capable of producing biosurfactant were isolated from oil-contaminated soils and seawater. Among them, the selected strain SW1 was identified as Pseudomonas sp. by taxonomical characteristic tests, and so tentatively named Pseudomonas sp. SWI. The optimal temperature and initial pH for biosurfactant production were TEX>30^{\circ}C.$ and 7.0, respectively. The optimal medium composilion for the production of biosurfactant by Pseudomonas sp. SW1 were hexadecane of 2.0%, yeast extract of 0.04%, $K_{2}HPO_4$ of 0.02%, $KH_2PO_4$ of 0.03% and $MgSO_4$ center dot $7H_2O$ of 0.04%, respectively. Under the above conditions, minimum wrface tension was 32 mN/m after incubation of 2 days. The biosurfactant was produced during initial stationary phase in the optimal medium. Pseudotnonas sp. SWl utilized various hydrocarbons such as Bunker oils, n-alkanes and branched alkanes as a sole carbon source.

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Component Analysis and Antioxidant Activity of Adenophora triphylla (잔대의 함유성분 분석과 항산화 활성)

  • Ham, Young-An;Choi, Hyun-Jin;Chung, Mi-Ja;Ham, Seung-Shi
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.3
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    • pp.274-279
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    • 2009
  • The levels of minerals, amino acids, fatty acids and sugars and antioxidant capacity of Adenophora triphylla (AT) root were investigated. The moisture, crude protein, crude lipid, crude ash and carbohydrate contents of the AT root were $3.4{\pm}0.3,\;9.4{\pm}0.1,\;1.6{\pm}0.1,\;3.7{\pm}0.2$, and $81.9{\pm}0.5$, respectively. Among the minerals present in root, potassium content was the highest. Total amino acid contents were $103.5{\pm}9.1$ mg/g. Palmitic acid was the most abundant fatty acid in AT root. The vitamin E and C levels in AT root were $1.1{\pm}0.1$ and $5.2{\pm}0.5$ mg/100 g, respectively. The antioxidant activity of 70% ethanol extract and fractions of the AT root has been determined by the scavenging of the stable radical DPPH; the result showed that the chloroform fraction was the most active, as the amount required for 50% reduction of DPPH after 30 min ($RC_{50}$) was $103.7{\mu}g$, followed by 70% ethanol extract ($265.4{\mu}g$), hexane fraction ($104.4{\mu}g$), ethyl acetate fraction ($104.4{\mu}g$), butanol fraction ($108.9{\mu}g$) and aqueous fraction ($484.1{\mu}g$).