• Journal of Applied Biological Chemistry
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• v.66
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• pp.98-104
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• 2023

Effective and alternative strategies to control methicillin-resistant Staphylococcus aureus (MRSA) are consistently needed. Previous study presented that 7,10-epoxy-octadeca-7,9-dienoic acid (EODA) was produced from 7,10-dihydroxy-8(E)-octadecenoic acid through one-step heat treatment. Further studies confirmed that EODA was highly active against broad range of pathogenic bacteria including MRSA, promising development of a novel antibacterial agent to control MRSA. However, there are some practical huddles for industrialization of EODA, especially high cost for fine purification. To address this problem, this study was focused on determination of any changes in the antibacterial activities of EODA when used as a crude extract. As a result, any significant changes in the antibacterial activities of EODA was not detected and additional synergistic effect for commercial antibiotics on antibacterial activity was sustained as it was.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.7
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• pp.889-895
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• 2007

This study investigated the quality characteristics of red ginseng cheonggukjang development by a various of concentration of red ginseng extract and powder to enhance its acceptability and functional properties. In the cheonggukjang added with red ginseng extract, viscous substances, reducing sugars, and fibrinolytic activity showed higher values by the addition of red ginseng extract. The standard crude saponin content of products containing red ginseng was evaluated, and found to be suitable in cheonggukjang added with 4% red ginseng extract. In the cheonggukjang added with red ginseng powder, viscous substances, reducing sugars, and fibrinolytic activity showed a similar tendency to the cheonggukjang added with red ginseng extract. Amino type nitrogen and ammonia type nitrogen decreased by increasing the addition of red ginseng powder, and the crude saponin content of cheonggukjang with the addition of 14% red ginseng powder was suitable, in terms of manufacturing standards for products containing red ginseng. Dependent on the addition time of red ginseng in cheonggukjang, viscous substances, reducing sugars, fibrinolytic activity, amino type nitrogen, and ammonia type nitrogen showed no differences based on the addition time; however, for crude saponin content, the cheonggukjang with red ginseng added before fermentation showed a higher saponin content than the cheonggukjang with red ginseng added after fermentation. The ginsenosides $Rg_{1}$, Re, Rf, $Rh_{1}$, $Rg_{2}$, $Rb_{1}$, Rc, $Rb_{2}$, Rd and $Rg_{3}$ were detected, but ginsenoside content did not show significant difference according to addition time. In conclusion, the addition amount of red ginseng in cheonggukjang for optimum fermentation was concentration of 4% (w/w), which showed the best results in quality characteristics.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.4
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• pp.317-323
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• 1993

In order to clarify the contents of active principles in floral inhibition cultured Angelica gigas roots, the amounts of crude extract and the contents of decursin and decursinol angelate in the yearly roots were analyzed and compared with respect to the root age, root part and growth stage. The woody cell, weight and width of pith and cortex were also investigated at different growth stage to observe the developmental characteristics of lignification in the roots as bolting and flowering in normal cultured Angelica gigas. The amount of crude extracts did not differ with plant ages, whereas the contents of decursin and decursinol angelate were differed and the highest in 3 year old roots. The contents of decursin in 1, 2 and 3 year old roots were 3.71, 4.76 and 8.20% and those of decursinol angel ate were 2.84, 3.40 and 5.01%, respectively. The amount of crude extracts, and the contents of decursin and decursinol angelate were the highest in fine roots, followed by the lateral roots and the lowest in the primary roots. On the other hand, the amount of the constituents in the cortex were much higher than those in the pith of the root. The amounts of crude extract, and the contents of decursin and decursinol angelate showed the highest value at the vagetative stage and decreased with development to bolting and blooming stage. Woody cells were accumulated in the pith of the root as advancing growth stage, so that the weight and radius of the pith increased, whereas the relative weight and width of the cortex decreased slightly.

• Applied Biological Chemistry
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• v.49 no.1
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• pp.77-81
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• 2006

This study was carried out to investigate the chemical components, and antioxidative and $anti-{\alpha}-glucosidase$ activities of Equisetum arvense extracts. In Equisetum arvense extracts were composed of 53.20% of crude fiber, 20.42% of crude ash, 15.32% of crude protein and 2.21% of crude fat. Potassium was the most predominant mineral and followed by phosphorus, calcium, magnesium, and sodium. The contents of the unsaturated fatty acids, such as linolenic acid, linoleic acid, and palmitic acid, were higher than those of saturated fatty acids. Seventy percent ethanol extract exhibited antioxidative activity with $IC_{50}$ of $168.1\;{\mu}g/ml$. The Seventy percent methanol extract showed higher ${\alpha}-glucosidase$ inhibitory activity than other solvent extracts.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.3
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• pp.416-420
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• 2009

An experiment of one-year duration was conducted on sixteen adult male German Angora rabbits under sub-temperate Himalayan conditions, to assess the effect of seasons on their body weight, wool production and quality, plane of nutrition and the digestibilities of nutrients. The daily meteorological attribute viz. minimum and maximum temperature; relative humidity and rainfall were recorded during winter (October to March), summer (April to June) and rainy (July to September) seasons. Biological parameters viz. body weight at the time of shearing, wool yield of individual rabbit, quality attributes of wool, fortnightly dry matter intake, chemical composition of feed and fodder and digestibilities of nutrients were recorded. Average minimum and maximum ambient temperature during winter, summer and rainy seasons were 4.6${\pm}$1.9 and 21.4${\pm}$2.8; 13.6${\pm}$2 and 30.3${\pm}$2; and 20.0${\pm}$1.4 and $31.0{\pm}1.8^{\circ}C$, respectively. The average relative humidity and total rainfall during winter, summer and rainy season were 69.5${\pm}$2.9% and 74.7${\pm}$21.8 mm; 58.6${\pm}$2.2% and 38.1${\pm}$18.1 mm; and 69${\pm}$4.2% and 104.0${\pm}$43.7 mm, respectively. The body weight of rabbits increased during all seasons, however, the maximum average daily weight gain of 3.47${\pm}$0.1 g was observed during the rainy season. The wool yield differed significantly (p$\leq$0.05) among different seasons with highest (140.4${\pm}$10 g) and lowest (108.5${\pm}$6.9 g) during winter and summer, respectively. The wool yield during the rainy season was 123.3${\pm}$5.2 g. The wool quality attributes revealed non-significant differences for staple length, fiber diameter, medulation percent, percent pure fibers and percent guard hairs. Plane of nutrition revealed significant (p$\leq$0.05) differences for concentrate intake. The concentrate intake was highest during winter (124.4${\pm}$2.6 g) followed by summer (86.8${\pm}$8.9 g) and rainy (80.7${\pm}$11.8 g) seasons. The reverse trend was observed in roughage intake with significantly (p${\leq}$0.05) lower intake during winter and highest during summer months. As a result total dry matter intake during different seasons was similar. Significant differences (p${\leq}$0.05) were observed for digestibilities of crude protein, crude fiber, ether extract, acid detergent fiber and cellulose. Digestibility of crude protein was highest during winter whereas the digestibilities of crude fiber, ether extract, acid detergent fiber and cellulose remained higher during the rainy season. During the winter season, the dry matter used for producing 100 g of wool was substantially lower than during other seasons and was concluded to be the best season for production of Angora wool under subtemperate Himalayan conditions.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.4
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• pp.522-527
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• 2002

The experiment was conducted for a period of 56 days with twelve Bangladeshi bull calves of average body weight of $127.20{\pm}11.34$ kg. The calves were divided into 3 groups having 4 animals in each. The animals were fed urea-treated rice straw designated as A) 4% urea-treated rice straw, B) 4% urea+4% soybean-treated rice straw and C) 4% urea+6% soybean-treated rice straw. In addition, all the animals were supplied 2 kg green grass, 350 g Til-oil-cake and 100 g common salt per 100 kg body weight of animals. Straw was treated with 4% urea solution and soybean meal at 4 and 6% were added to treated straw and kept for 48 h in double layer polythene bags under anaerobic condition. Urea treatment improved crude protein (CP) content of rice straw from 2.68 to 8.70% and it was further increased by 10.74 and 12.12% with the addition of 4 and 6% soybean meal. Dry matter (DM) intake (kg) was higher (p<0.05) in C (4.2) followed by B (4.1) and A (4.0). Crude protein intake was significantly higher (p<0.05) in group B and C than group A. Total live weight gains were 20.2, 24.8 and 25.6 kg for calves of group A, B and C respectively (p<0.01). The addition of soybean meal to treated rice straw did not affect the coefficients of digestibility of DM, OM, EE and NFE. However, CP and CF digestibility were significantly higher in group B and C (p<0.05). The values for digestible crude protein (DCP), digestible ether extract (DEE), digestible nitrogen free extract (DNFE) and total digestible nutrients (TDN) were significantly (p<0.05) higher in diet C and B in comparison to diet A, but there were no significant difference in digestible organic matter (DOM) and digestible crude fibre (DCF) value among the groups. It may be concluded that 4% urea treated rice straw can be fed to growing bull calves with 2 kg green grass and a small quantity of concentrate without any adverse effect on feed intake and growth. Moreover, soybean meal at 4 and 6% can be added to urea treated rice straw at the time of treatment for rapid hydrolyzing of urea, which resulted an improvement in nutrient digestibility and better utilization of rice straw for growth of growing bull calves.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.6
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• pp.838-844
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• 2013

This study was conducted to evaluate chemical composition, nitrogen-corrected true metabolizable energy (TMEn) and true amino acids digestibility of corn distillers dried grains with solubles (DDGS) produced in China. Twenty five sources of corn DDGS was collected from 8 provinces of China. A precision-fed rooster assay was used to determine TMEn and amino acids digestibility with 35 adult cecectomized roosters, in which each DDGS sample was tube fed (30 g). The average content of ash, crude protein, total amino acid, ether extract, crude fiber and neutral detergent fiber were 4.81, 27.91, 22.51, 15.22, 6.35 and 37.58%, respectively. TMEn of DDGS ranged from 1,779 to 3,071 kcal/kg and averaged 2,517 kcal/kg. Coefficient of variation for non-amino acid crude protein, ether extract, crude fiber and TMEn were 55.0, 15.7, 15.9 and 17.1%, respectively. The average true amino acid digestibility was 77.32%. Stepwise regression analysis obtained the following equation: TMEn, kcal/kg = -2,995.6+0.88${\times}$gross energy+$49.63{\times}a^*$ (BIC = 248.8; RMSE = 190.8; p<0.01). Removing gross energy from the model obtained the following equation: TMEn, kcal/kg = 57.88${\times}$ether extracts+$87.62{\times}a^*$ (BIC = 254.3, RMSE = 223.5; p<0.01). No correlation was found between color scores and lysine true digestibility (p>0.05). These results suggest that corn DDGS produced in China has a large variation in chemical composition, and gross energy and $a^*$ value can be used to generate TMEn predict equation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.1
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• pp.23-30
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• 2002

Eveuadies 71' kenaf (Hibiscus cannabinus L.) was grown at five plant densities (1, 2, 3, 4, and 5 plants per hill; 16,000, 32,000, 48,000, 64,000 and 80,000 plants/10a) from April 13 to October 8, 2001 in Jeju island to determine influence of plant number per hill on agronomic characteristics, forage yield and quality. As plant density increased from 1 to 5 plants per hill, the plant height averaged across two harvests increased 176 to 185 cm. Compared with the plant density of two plants per hill, the plant height decreased at higher plant densities (183, 180, and 166 cm for 3, 4 and 5 plants per hill). The highest yield of fresh forage (114.6 MT/ha), dry matter (17.3 MT/ha), crude protein (2.33 MT/ha), and total digestible nutrients (TDN) (9.21 MT/ha) was obtained at 2 plants per hill. As plant density increased from 1 to 5 plants per hill, crude protein, ether extract, nitrogen free extract, and TDN contents increased from 13.1 to 15.4%, 3.0 to 3.7%, 20.1 to 23.2% and 41.1 to 45.4%, respectively, while crude fiber and crude ash contents decreased from 41.2 to 34.9% and 9.4 to 8.2%, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.1
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• pp.15-22
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• 2002

This study was conducted from April 13 to October 8, 2001 in Jeju to determine the influence of cutting height (2, 4, 6, 8 and 10 cm from the soil surface) on growth, forage yield and chemical composition of kenaf (Hibiscus cannabinus L.). As cutting height was increased from 2 to 10 cm, plant height averaged across two cuttings increased from 157.7 to 184.7 cm. This pattern held fir the number of leaves and branches per plant, stem diameter and plant weight per plant. As cutting height increased from 2 to 10 cm, fresh forage, dry matter, crude protein and TDN yields increased from 85.5 to 113.7MT/ha, from 11.97 to 15.63 MT/ha, from 1.63 to 2.72, and from 4.95 to 7.54 MT/ha, respectively. As cutting height was increased from 2 cm to 10 cm, crude protein, ether extract, nitrogen free extract, and TDN contents increased from 14.2 to 17.6%, 2.9 to 3.9%, 24.2 to 25.8% and 43.1 to 48.5%, respectively, while crude fiber and crude ash contents decreased from 35.5 to 30.4 % and 9.9 to 8.1 %, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.1
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• pp.9-14
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• 2002

Evegradies 71' kenaf (Hibiscus cannabinus L.) was grown at 160,000 plants per ha in a volcanic ash soil in Jeju island to determine the optimum frequency of split N application fer forage production. Nitrogen fertilizer was applied with 240 kg/ha, and frequencies of the split N applications were 1, 2, 3, 4 and 5 times. Plant height averaged across two harvests increased from 187 to 201cm as N was split-applied from one to three applications and then decreased to 201cm in five applications. This pattern held for the number of branches and leaves per plant stem diameter and weight of plant per plant. Fresh forage yield increased from 91.8 to 114.2 MT/ha. dry matter yield from 12.70 to 16.6 MT/ha, crude protein yield from 1.75 to 2.48MT/ha, and total digestible nutrients (TDN) yield from 5.39 to 7.63 MT/ha as N was split-applied from one to four applications, and then decreased to 106.6, 15.0, 2.32, and 7.22 MT/ha in five applications, respectively As N was split-applied from one to five applications, crude protein content increased from 13.8 to 15.4%. ether extract content from 4.1 to 5.9%, and TDN content from 42.4 to 48.1%. but crude fiber decreased from 38.2 to 37.1% and crude ash content from 11.5 to 8.6%. Nitrogen free extract content was about 19.0% regardless of cutting height.