• Title/Summary/Keyword: crude extract

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Studies on the browning inhibition of yam(Dioscorea aimadoimo) during hot air dehydration (단마(Dioscorea aimadoimo)의 열풍건조 시 갈변 억제 방안 연구)

  • Chung, Yong-Yul;Jeong, Woo-Sik;Chung, Shin-Kyo
    • Applied Biological Chemistry
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    • v.39 no.5
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    • pp.384-388
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    • 1996
  • This research was conducted to investigate the effective methods for browning inhibition on yam (Dioscorea aimadeimo) during dehydration by physical and chemical pretreatments. Moisture, crude protein, crude fiber and N-free extract contents of yam were 81.17%, 1.43%, 0.29% and 15.81%, respectively. Yams were sliced to 0.5 cm thickness and placed to single and poly layer in plastic tray, and then changes of their weights were measured during air dehydration at $50^{\circ}C,\;65^{\circ}C,\;and\;80^{\circ}C$. The dehydration time reaching to optimum moisture level for the pulverization of the yam slices were 10, 6, 3 hours(single layered) and 12, 7, 5 hours(multi layered) at the respective temperature. To inhibit browning at $80^{\circ}C$ air dehydration, water and steam blanching, microwave treatment effects were investigated on yam slices for 30 sec. and 60 sec. Steam blanching for 30 sec. was comparatively effective to inhibit browning of yam slices. Yam slices were immersed in single and combined browning inhibitor solutions and evaluated for browing degree during dehydration by the values of Hunter L, a, b and ${\Delta}E$. The most effective pretreatment to inhibit browning of yam slices was immersion In the solution containing 500 ppm of citric acid and 1000 ppm of cysteine for 1 min.

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Physicochemical quality characteristics of hot water extracts of processed ginseng based on different heat treatments (열처리 방법에 따른 가공 인삼 열수추출물의 이화학적 품질 특성)

  • Kang, Yoon-Han;Zhou, Rui;Kim, Hyo Jin;Kim, Ji Eun;Shin, Il Shik
    • Food Science and Preservation
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    • v.25 no.1
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    • pp.155-163
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    • 2018
  • The present study was carried out to investigate the physicochemical properties of hot water extracts of red ginseng powder prepared by two-stage hot air drying method using steamed ginseng and steaming liquid for 2.5 h under high-temperature and high-pressure autoclave condition. The total polyphenols, total flavonoids, total sugar, acid polysaccharides and crude saponin in hot water extracts from red ginseng powder were analyzed and determined, and the flavor components of ginseng were measured using color difference meter and an electronic tongue. The total polyphenol, total flavonoid, total polysaccharide, and acid polysaccharide of the red ginseng hot water extract obtained by autoclaving (ARG) were 9.06 mg GAE/g, 3.38 mg NE/g, 35.22 g/100 g, and 10.90 g/100 g, respectively. The final contents of the total polyphenols, total flavonoids, crude saponin were higher than those determined using other red ginseng methods; the time required for steamed red ginseng production reduced. The total ginsenoside content of ginseng including Rb1 was 10.69 mg/g, which is the lowest ARG. The processing conditions affected the conversion to ginsenosides unique to red ginseng. Red ginseng and steaming liquid obtained from the autoclave are expected to be in need for non-food materials and products as well as foods by improving the flavor components through conversion of red ginseng components into low molecular weight.

Chemical Constituents of Bulb of Lilium lancifolium Thunberg and Lilium tsingtauense Gilg (참나리와 하늘말나리 인경의 성분분석)

  • Park, Tae Young;Seo, Kyoung-Sun;Choi, Seongkyu;Yun, Kyeong Won
    • Korean Journal of Plant Resources
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    • v.27 no.2
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    • pp.125-132
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    • 2014
  • In this study, the chemical constituents of bulb from Lilium lancifolium and Lilium tsingtauense were investigated. The content of moisture, crude protein and fiber of bulb from L. tsingtauense was higher than that of L. lancifolium, whereas the content of crude ash and nitrogen free extract of bulb from L. lancifolium was higher than that of L. tsingtauense. HPLC analysis revealed that total free sugar and amino acid content of bulb from L. tsingtauense was higher than that of L. lancifolium. There was no remarkable difference in proximate composition between the two species. The content of p-coumaric acid and ferulic acid was 1.93 mg% and 0.25 mg% in ethyl acetate fraction of L. lancifolium, on the other hand, 2.94 mg% and 0.46 mg% in ether fraction of L. tsingtauense. These results suggest that the bulb of L. tsingtauense can be considered as promising oriental medicine resource likewise L. lancifolium.

Analysis of Helical Pile Behavior in Sands Varying Helix Pitch Based on Numerical Analysis Results (사질토에 근입된 헬릭스 피치에 따른 헬리컬 파일의 수치해석적 거동분석)

  • Bak, Jongho;Lee, Kicheol;Choi, Byeong-Hyun;Kim, Dongwook
    • Journal of the Korean Geosynthetics Society
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    • v.17 no.4
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    • pp.29-40
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    • 2018
  • Oil sands, which are largely distributed in Canada and Venezuela, are a mixture of crude oil and sandy soils. In order to extract crude oil from oil sands, construction of massive oil sand plants is required. Generally, the typically-used foundation types of the oil sand plant are driven piles and cast-in-place piles. Most of the oil sand plants are located in cold and remote regions. Installation of driven piles in frozen or organic surface soils is difficult due to high resistance and installation equipment accessability, while the cast-in-place pile has concrete curing problem due to cold temperature. Helical pile can be installed quickly and easily using rotation with a little help of vertical load. As the installation of helical pile is available using a small and light-weight installation equipment, accessibility of installation equipment is improved. The helical pile has an advantage of easy removal by rotation in reverse direction compared with that of installation. Furthermore, reuse of removed helical piles is possible when the piles are structurally safe. In this study, the behavior of helical piles varying helix pitch was analyzed based on the numerical analysis results. Numerical model was calibrated based on the results of model helical pile tests in laboratory. The ultimate helical pile loads, the displacement of each helix attached to the shaft of the helical pile, and the load sharing ratio of each helix were analyzed.

Anti-invasion Effects of Calystegia soldanella Solvent Extracts and Partitioned Fractions on PMA-stimulated Fibrosarcoma Cells (갯메꽃 추출물과 유기용매 분획물의 암전이 억제 효과)

  • Son, Jaemin;Kim, Junse;Kim, Hojun;Seo, Youngwan
    • Journal of Life Science
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    • v.29 no.3
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    • pp.287-294
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    • 2019
  • Calystegia soldanella is distributed in coastal sand dunes and has high environmental adaptability; it is also known to be effective for anti-oxidant, anti-pyretic, anti-septic, and diuretic action. This study investigated the effect of crude extracts and organic solvent fractions of C. soldanella on MMP-2 and MMP-9 expression, MMP activity, and cell mobility in phorbol-12-myristate-13-acetate (PMA)-induced fibrosarcoma HT-1080 cells. C. soldanella was twice extracted, once with methylene chloride (MC) and once with methanol (MeOH). After the MC and MeOH extracts were combined, their suppressive effects on MMP-2 and MMP-9 expression, MMP enzymatic activity, and gene and protein expression were measured by gelatin zymography, enzyme-linked immunosorbent assay, reverse-transcription polymerase chain reaction, and western blot method. Cell mobility for the HT-1080 cells was observed by wound healing assay. The combined crude extracts showed a significant suppressive effects on MMP-2 and MMP-9 expression. To explore active inhibitory elements, the combined extracts were fractionated according to polarity into with n-hexane, 85% aqueous methanol, n-butanol, and water. Across these four solvent fractions, MMP-2 and MMP-9 activity and cell mobility in the HT-1080 cells were all strongly inhibited by the n-hexane fraction. These results suggest that C. soldanella extract and organic solvent fractions could be used as potent MMP inhibitors for effective anti-cancer treatments to suppress cancer invasion and metastasis.

Immunomodulatory and anti-metastatic activities of polysaccharide isolated from red cabbage (적양배추에서 분리한 다당의 면역 및 항전이 활성)

  • Lee, Sue Jung;Shin, Kwang-Soon
    • Korean Journal of Food Science and Technology
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    • v.51 no.3
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    • pp.263-271
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    • 2019
  • In this study, we examined the immunostimulating characteristics of a hot water extract (RCW) and crude polysaccharides (RCP) of red cabbage. RCW and RCP did not show any cytotoxicity in B16BL6 cells and macrophages. Although the sugar compositions of RCW and RCP were similar, the uronic acid content of RCP was higher than that of RCW RCP significantly increased the production of various cytokines and NO, whereas RCW did not affect the production of cytokines and NO. In an ex vivo assay of natural killer (NK) cell activity, intravenous (i.v.) administration of RCP significantly augmented NK cytotoxicity against Yac-1 tumor cells at 3 days after RCP treatment. In an experimental lung metastasis model using B16BL6 melanoma cells, i.v. administration of RCP at a dose of $1,000{\mu}g$ per mouse significantly inhibited 47.3% of lung metastasis. These results suggest that crude polysaccharide isolated from red cabbage is a promising food ingredient for the prevention of tumor metastasis.

Effects of Microbial Additives and Silo Density on Chemical Compositions, Fermentation Indices, and Aerobic Stability of Whole Crop Rice Silage (미생물 첨가와 사일로 밀도가 총체벼 사일리지의 영양소 함량, 발효특성 및 호기적 안전성에 미치는 영향)

  • Joo, Young Ho;Jeong, Seung Min;Seo, Myeong Ji;Lee, Seong Shin;Choi, Ki Choon;Kim, Sam Churl
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.42 no.2
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    • pp.96-102
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    • 2022
  • The present study investigated effects of microbial additives and silo density on chemical compositions, fermentation indices, and aerobic stability of whole crop rice (WCR) silage. The WCR ("Youngwoo") was harvested at 49.7% dry matter (DM), and ensiled into 500 kg bale silo with two different compaction pressures at 430 kgf (kilogram-force)/cm2 (LOW) and 760 kgf/cm2 (HIGH) densities. All WCR forage were applied distilled water (CON) or mixed inoculants (Lactobacillus brevis 5M2 and Lactobacillus buchneri 6M1) with 1:1 ratio at 1x105 colony forming unit/g (INO). The concentrations of DM, crude protein, ether extract, crude ash, neutral detergent fiber, and acid detergent fiber of whole crop rice before ensiling were 49.7, 9.59, 2.85, 6.74, 39.7, and 21.9%, respectively. Microbial additives and silo density did not affect the chemical compositions of WCR silage (p>0.05). The INO silages had lower lactate (p<0.001), but higher propionate (p<0.001). The LOW silages had higher lactate (p=0.004). The INO silages had higher yeast count (p<0.001) and aerobic stability (p<0.001). However, microbial counts and aerobic stability were not affected by silo density. Therefore, this study concluded that fermentation quality of WCR silage improved by microbial additives, but no effects by silo density.

Meat quality, post-mortem proteolytic enzymes, and myosin heavy chain isoforms of different Thai native cattle muscles

  • Chaosap, Chanporn;Sivapirunthep, Panneepa;Sitthigripong, Ronachai;Tavitchasri, Piyada;Maduae, Sabaiporn;Kusee, Tipyaporn;Setakul, Jutarat;Adeyemi, Kazeem
    • Animal Bioscience
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    • v.34 no.9
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    • pp.1514-1524
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    • 2021
  • Objective: This study investigated the meat quality characteristics, endogenous proteolytic enzymes, collagen content, and myosin heavy chain (MyHC) isoforms of different muscles of Thai native cattle (TNC). Methods: Infraspinatus (IF), Longissimus thoracis (LT), and Supraspinatus (SS) muscles were obtained from two TNC breeds, Kho-Lan (KL, n = 7) and Kho-Isaan (KI, n = 7). The muscle and meat characteristics of TNC breeds and their relationship with MyHC expression were examined. Results: Three MyHC isoforms namely MyHC I, MyHC IIa, and MyHC IIx were detected in the muscles. The KL had higher (p<0.05) MyHC IIx than the KI. The IF muscle had higher (p<0.05) MyHC I compared to other muscles. The LT muscle had the least MyHC I. The LT had higher (p<0.05) MyHC IIx than the IF and SS muscles. The IF presented the least MyHC IIx. The KL had higher (p<0.05) lightness and moisture content and lower crude protein, redness, cooking loss, shear force, and calpastatin than the KI. The glycogen, total collagen, soluble collagen, crude protein, ash contents, and troponin T degradation product of IF and SS were lower (p<0.05) than that of LT. Ether extract in LT was lower (p<0.05) than that of IF and SS. The percentage of MyHC I, MyHC IIa, and MyHC IIx were significantly correlated with muscle and meat characteristics of TNC. Conclusion: These results suggest that the differences in the MyHC isoforms may partly account for the variation in meat quality between breeds and among muscles of TNC.

Forage Yield and TDN by Cutting Time of Brittle Culm Rice (Brittle Culm 벼의 예취시기에 따른 청예수량 및 TDN)

  • Kim, Young-Doo;Park, Hong-Kyu;Ha, Ki-Yong;Cho, Soo-Yeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.4
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    • pp.483-488
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    • 1997
  • This experiment was conducted to determine cutting time on the yield and nutritive value of brittle culm rice. Plant height and number of tiller were significantly different between variety and cutting time, and regrowth plant height and rate of regrowth tiller were tall and high at the early cutting. Early cutting increased crude protein, fat and NFE(Nitrogen free extract) content in the harvested foliage but crude fiber and ash content were decreased in the first cutting. Those of second cutting also showed reverse tendency. Fresh and dry matter yield were highest on heading date cutting, and those of KL501 were higher than those of Seomjinbyeo. TDN(Total digestible nutrients) content was higher with earlier cutting for initiated cutting, but that of second cutting was reversed. KL501 showed higher TDN content than Seomjinbyeo did at the any cutting time. TDN yield of heading date cutting was higher than that of the other cutting time.

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Effects of Forage Cutting and Baler Mixing on Chemical Compositions, Fermentation Indices, and Aerobic Stability of Whole Crop Rice Haylage (조사료의 세절과 베일러 내 교반이 총체벼 헤일리지의 영양소 함량, 발효특성 및 호기적 안전성에 미치는 영향)

  • Myeong Ji Seo;Young Ho Joo;Seong Shin Lee;Ji Yoon Kim;Chang Hyun Baeg;Seung Min Jeong;Ki Choon Choi;Sam Churl Kim
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.1
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    • pp.50-55
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    • 2023
  • The present study investigated the effects of forage cutting and baler mixing on the chemical compositions, fermentation indices, and aerobic stability of whole crop rice (WCR) haylage. The WCR ("Youngwoo") was harvested at 48.4% dry matter and ensiled into a 300 kg bale silo with forage cutting (whole crop without cutting vs. 5 cm of cutting length). The WCR forages were ensiled without baler mixing process (CON) or with (MIX). The concentrations of dry matter, crude protein, ether extract, crude ash, neutral detergent fiber, and acid detergent fiber of whole crop rice before ensiling were 48.4, 9.70, 2.57, 6.11, 41.2, and 23.5%, respectively. The forage cutting did not affect the chemical compositions, fermentation indices, microbes, and aerobic stability of WCR haylage (p>0.05). The CON haylages tend to be higher in NDF content (p<0.10). The MIX haylages had lower in lactate (p=0.019), and lactate:acetate ratio (p<0.001). The MIX haylages had higher in lactic acid bacteria (LAB) (p=0.010). Therefore, this study concluded that the fermentation quality of WCR haylage improved by baler mixing, but had no effects by forage cutting.