• 제목/요약/키워드: crtI

검색결과 61건 처리시간 0.028초

Metabolic engineering of Lilium ${\times}$ formolongi using multiple genes of the carotenoid biosynthesis pathway

  • Azadi, Pejman;Otang, Ntui Valentaine;Chin, Dong Poh;Nakamura, Ikuo;Fujisawa, Masaki;Harada, Hisashi;Misawa, Norihiko;Mii, Masahiro
    • Plant Biotechnology Reports
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    • 제4권4호
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    • pp.269-280
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    • 2010
  • Lilium ${\times}$ formolongi was genetically engineered by Agrobacterium-mediated transformation with the plasmid pCrtZW-N8idi-crtEBIY, which contains seven enzyme genes under the regulation of the CaMV 35S promoter. In the transformants, ketocarotenoids were detected in both calli and leaves, which showed a strong orange color. In transgenic calli, the total amount of carotenoids [133.3 ${\mu}g/g$ fresh weight (FW)] was 26.1-fold higher than in wild-type calli. The chlorophyll content and photosynthetic efficiency in transgenic orange plantlets were significantly lowered; however, after several months of subculture, they had turned into plantlets with green leaves that showed significant increases in chlorophyll and photosynthetic efficiency. The total carotenoid contents in leaves of transgenic orange and green plantlets were quantified at 102.9 and 135.2 ${\mu}g/g$ FW, respectively, corresponding to 5.6- and 7.4-fold increases over the levels in the wild-type. Ketocarotenoids such as echinenone, canthaxanthin, 3'-hydroxyechinenone, 3-hydroxyechinenone, and astaxanthin were detected in both transgenic calli and orange leaves. A significant change in the type and composition of ketocarotenoids was observed during the transition from orange transgenic plantlets to green plantlets. Although 3'-hydroxyechinenone, 3-hydroxyechinenone, astaxanthin, and adonirubin were absent, and echinenone and canthaxanthin were present at lower levels, interestingly, the upregulation of carotenoid biosynthesis led to an increase in the total carotenoid concentration (+31.4%) in leaves of the transgenic green plantlets.

나눗셈 체인을 이용한 RSA 모듈로 멱승기의 구현 (Implementation of RSA modular exponentiator using Division Chain)

  • 김성두;정용진
    • 정보보호학회논문지
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    • 제12권2호
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    • pp.21-34
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    • 2002
  • 본 논문에서는 최근 발표된 멱승방법인 나눗셈 체인을 적용한 새로운 모듈로 멱승기의 하드웨어 구조를 제안하였다. 나눗셈 체인은 제수(divisor) d=2 또는 $d=2^I +1$ 과 그에 따른 나머지(remainder) r을 이용하여 지수 I를 새롭게 변형하는 방법으로 전체 멱승 연산이 평균 약 1.4$log_2$E 번의 곱셈으로 가능한 알고리즘이다. 이것은 Binary Method가 하드웨어 구현 시 항상 worst case인 $2log_2$E의 계산량이 필요한 것과 비교할 때 상당한 성능개선을 의미한다. 전체 구조는 파이프라인 동작이 가능한 선형 시스톨릭 어레이 구조로 설계하였으며, DG(Dependence Graph)를 수평으로 매핑하여 k비트의 키 사이즈에 대해 두 개의 k 비트 프레임이 k/2+3 개의 PE(Processing Element)로 구성된 두 개의 곱셈기 모듈을 통해 병렬로 동시에 처리되어 100% 처리율을 이루게 하였다. 또한, 규칙적인 데이터 패스를 가질 수 있도록 나눗셈체인을 새롭게 코딩하는 방법을 제안하였다. ASIC 구현을 위해 삼성 0.5um CMOS 스탠다드 셀 라이브러리를 이용해 합성한 결과 최장 지연 패스는 4.24ns로 200MHz의 클럭이 가능하며, 1024비트 데이터 프레임에 대해 약 140kbps의 처리속도를 나타낸다. 복호화 시에는 CRT(Chinese Remainder Theorem)를 적용하여 처리속도를 560kbps로 향상시켰다. 전자서명의 검증과정으로 사용되기도 하는 암호화 과정을 수행할 때 공개키 E는 3,17 혹은 $2^{16} +1$의 사용이 권장된다는 점을 이용하여 E를 17 비트로 제한할 경우 7.3Mbps의 빠른 처리속도를 가질 수 있다.

펄스 반사법을 이용한 NDT 시스템 (A NDT System using the Pulse-echo Method)

  • 반차식;김건;차일환;임광호
    • 한국음향학회지
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    • 제5권1호
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    • pp.35-40
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    • 1986
  • 본 연구는 펄스 반사법을 이용하여 금속 재료 단면의 영상을 얻는데 그 목적이 있다. 기존의 탐 상기는 대부분 A - 스캔 방식으로 결과를 전시하여 검사자가 직접 CRT 상에 나타난 결과를 판독해야 하므로 숙련자라 하더라도 어느정도 오차가 수반되며 판독에 시간이 요하는 등 불편이 따른다. 본 연구 에서는 판독 과정을 자동화함으로써 정확도와 검사 속도를 개선하였으며 탐촉 지점은 스텝핑모터를 이 용하여 일정 간격으로 제어하였다. 도 탐상 결과를 B-스캔 방식으로 영상 처리함으로써 재료 단면의 상 태 파악을 용이하게 하였다.

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The Efficient Production on single- and Multi- Wall Carbon Nanotubes

  • Shinohara, H.
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2000년도 제18회 학술발표회 논문개요집
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    • pp.207-207
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    • 2000
  • Multi- and single-wall carbon nanotubes are promising new carbon materials in nano-electronics, field-emitters, CRT-displays, hydrogen storage materials, biomedical tracers and so forth. The present talk will deal with a high-yield synthesis on quasi-aligned multi-wall carbon nanotubes via a chemical vapor deposition technique. I will also talk about a possible growth mechanism on single-wall carbon nanotubes based on newly obtained experimental results.

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유전 알고리즘을 이용한 SS형 편향코일의 형상 최적화 (The Optimization Of SS-Type Deflection Yoke By Using Genetic Algorithm)

  • 주관정;윤인중;강병훈;조명철;한송엽;이홍배
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1993년도 하계학술대회 논문집 B
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    • pp.971-973
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    • 1993
  • Deflection Yoke(the following, DY) is the important electric device of CRT which deflects R, G, B beans influencing magnetic field produced by yoke coils. Recently, DY is designed to the saddle/saddle type of coils, being proposed for high-definite and high-efficient CRT. This paper presents the optimization of pin-sectioned saddle coil's shape for minimizing gap between desired and practical deflections of electron beams by using Genetic Algorithm. Evolution Startegy is utilized in this paper, since evolution strategy is a kind of genetic algorithms finding the optimized values by choicing the better generation with comparing the parents and their children. Here, the children are generated by only mutations from the normal random variables. Evolution strategy has shown better powerful converge rate than the other genetic algorithms becuase of using only the mutation-operator.

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Benefit of volumetric-modulated arc therapy over three-dimensional conformal radiotherapy for stage I-II extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue in the stomach: a dosimetric comparison

  • Chung, Joo-Hyun;Na, Kyoungsu;Kim, Il Han
    • Radiation Oncology Journal
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    • 제36권4호
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    • pp.332-340
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    • 2018
  • Purpose: To retrospectively analyze dosimetric parameters of volumetric-modulated arc therapy (VMAT) and three-dimensional conformal radiotherapy (3D-CRT) delivered to extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue in the stomach (gastric MALT lymphoma) to find out advantages of VMAT and conditions for definite benefits of VMAT. Materials and Methods: Fifty patients with stage I-II gastric MALT lymphoma received VMAT (n = 14) or 3D-CRT (n = 36) between December 2005 and April 2018. Twenty-seven patients were categorized according to whether the planning target volume (PTV) overlaps kidney(s). Dosimetric parameters were analyzed by dose-volume histogram. Results: Radiation dose to the liver was definitely lower with VMAT in terms of mean dose (p = 0.026) and V15 (p = 0.008). The V15 of the left kidney was lower with VMAT (p = 0.065). For those with PTV overlapping kidney(s), the left kidney V15 was significantly lower with VMAT. Furthermore, the closer the distance between the PTV and kidneys, the less the left kidney V15 with VMAT (p = 0.037). Delineation of kidney(s) by integrating all respiratory phases had no additional benefit. Conclusions: VMAT significantly increased monitor units, reduced treatment time and radiation dose to the liver and kidneys. The benefit of VMAT was definite in reducing the left kidney V15, especially in geometrically challenging conditions of overlap or close separation between PTV and kidney(s).

토마토 과색 돌연변이 유전자(old-gold-crimson) 선발을 위한 dCAPS 분자표지 개발 (A Gene-based dCAPS Marker for Selecting old-gold-crimson (ogc) Fruit Color Mutation in Tomato)

  • 박영훈;이용재;강점순;최영환;손병구
    • 생명과학회지
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    • 제19권1호
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    • pp.152-155
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    • 2009
  • old-gold-crimson ($og^c$) 과색 돌연변이는 라이코펜의 함량이 증가된 진붉은색 토마토 과실을 생산한다. 이러한 돌연변이는 토마토의 carotenoid 생합성경로에 관여하여 라이코펜을 ${\beta}$-carotene으로 전환시키는 라이코펜 ${\beta}$-cyclase (Crt-b) 유전자(B)에 point mutation을 일으켜 정상적인 효소생성을 저해한다. 높은 함량의 라이코펜을 생성시키는 토마토 품종개발은 유전자 연관 DNA 마커를 이용한 분자표지이용선발(MAS)을 통해 가속화 될 수 있다. $og^c$ 돌연변이는 라이코펜 ${\beta}$-cyclase(Crt-b) 유전자 내 poly-A 서열반복 지점에서 adenine (A) 단일 뉴클레오티드의 결손에 의한 frame-shift mutation에 의해 일어나며, 이러한 대립유전자의 운영과 검증을 위해 $og^c$ 대립유전자로부터 합성되는 PCR 산물에 Hin fI 제한효소 인식부위가 인위적으로 생성되도록 PCR 프라이머에 단일 뉴클레오티드 mismatch 부위를 만들어 dCAPS 마커를 개발하였다. 본 dCAPS 마커는 유전자 유래의 공우성 PCR 마커로서 고함량 라이코펜 토마토개발을 위한 육종 프로그램의 MAS에 효과적으로 사용될 수 있다.

Maskless용 스크린 제판 기술 연구(I) (A Study on the Maskless Plate Making Technology for Screen Printing(I))

  • 이미영;박경진;남수용
    • 한국인쇄학회지
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    • 제26권1호
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    • pp.73-85
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    • 2008
  • We have manufactured a photoresist which has excellent dispersity and good applying property due to 330cps of viscosity for environment-friendly and economical maskless screen plate making. And the photoresist applied on the screen stretched was exposed without mask by beam projector with CRT light source. Then it was developed by air spray with $1.7kgf/cm^2$ of injection pressure. The pencil hardness and solvent resistance of curing photoresist film were worse than those of conventional photoresist film and the maximum resolution of line image formed by maskless screen plate making was 0.5 mm since the exposure system for maskless plate making has weak light intensity and the diffusion of light. But we could obtain maskless screen plate which has sharp edges of line image and confirm a possibility of dry development process by air spray method.

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목재건조(木材乾燥)의 자동화(自動化)에 관한 연구(硏究)(I) -연속온도상승(連續溫度上昇)스케쥴을 이용한 목재건조장치(木材乾燥裝置) 자동화(自動化)- (Automation of Lumber Drying System(I) -Continuously Rising Temperature Drying of Pinus densiflora-)

  • 이형우
    • Journal of the Korean Wood Science and Technology
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    • 제22권1호
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    • pp.12-19
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    • 1994
  • An electrically heated experimental lumber dry kiln was retrofitted with a computer-based control system to control kiln conditions more precisely and monitor and record several kiln variables. Flat-sawn 2.5cm-thick Pinus densiflora boards were dried in constant temperature process(65$^{\circ}C$ & 50~60 %RH) and continuously rising temperature process, respectively. The average drying rate in continuously rising temperature process was 5.7 %/hr, which was above 3 times faster than that in constant temperature process. But, the average rate of case-hardening and moisture difference between shells and cores of boards dried in continuously rising temperature process were 82 % and 5.5 %, respectively, which were much larger than those of boards dried in constant temperature process.

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