• Title/Summary/Keyword: crtI

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Transcript accumulation of carotenoid biosynthesis genes in the cyanobacterium Synechocystis sp. PCC 6803 during the dark-to-light transition is mediated by photosynthetic electron transport

  • Ryu, Jee-Youn;Song, Ji-Young;Chung, Young-Ho;Park, Young-Mok;Chow, Wah-Soon;Park, Youn-Il
    • Plant Biotechnology Reports
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    • v.4 no.2
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    • pp.149-155
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    • 2010
  • Expression of the genes for carotenoid bio-synthesis (crt) is dependent on light, but little is known about the underlying mechanism of light sensing and signalling in the cyanobacterium Synechocystis sp. PCC 6803 (hereafter, Synechocystis). In the present study, we investigated the light-induced increase in the transcript levels of Synechocystis crt genes, including phytoene synthase (crtB), phytoene desaturase (crtP), ${\zeta}$-carotene desaturase (crtQ), and ${\beta}$-carotene hydroxylase (crtR), during a darkto-light transition period. During the dark-to-light shift, the increase in the crt transcript levels was not affected by mutations in cyanobacterial photoreceptors, such as phytochromes (cph1, cph2 and cph3) and a cryptochrome-type photoreceptor (ccry), or respiratory electron transport components NDH and Cyd/CtaI. However, treatment with photosynthetic electron transport inhibitors significantly diminished the accumulation of crt gene transcripts. Therefore, the light induction of the Synechocystis crt gene expression is most likely mediated by photosynthetic electron transport rather than by cyanobacterial photoreceptors during the dark-to-light transition.

Printability according to Graining of Plate in the Lithography (I) (평판인쇄에 있어서 모랫발 형성에 따른 인쇄적성 (I))

  • 손세모
    • Journal of the Korean Graphic Arts Communication Society
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    • v.12 no.1
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    • pp.57-68
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    • 1994
  • In the last decade, digital image processing technics have been rapidly developed according to the advancement of electronic technique, and total scanner and DTP system were developed. Specially, there is need to retouch the color and image on the CRT. In this paper, we described a method to display surface color of printing on the CRT, and an algorithm to simulate the color reproduction by offset printing. The experimental results show that the described method and algorithm are useful and valid on the computer simulation of the multi-color printing adapted process ink characteristics.

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A Study on Parallel-mode CRT Interfacting Technique in Microcomputer System and Prototype Program development System (마이크로 컴퓨터의 병렬형 CRT 입출력 방식과 Prototype Program development System에 관한 연구)

  • 정창경
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.14 no.4
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    • pp.9-14
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    • 1977
  • A parallel mode CRT interfacing technique has been suggested for a microcomputer system. The number of components and the I/O speed were improved significantly by this technique. As an appliation of this technique, a prototype program develoment system was designed and tested experimentally for the use fulness in prototype program developmenting.

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A Study on the Structural Design Approach to Improve Shockproof Characteristic in Cathode Ray Tube (음극선관의 내충격 특성 향상을 위한 구조 설계에 관한 연구)

  • Park, Sang-Hu;Kim, Won-Jin;Lee, Boo-Youn
    • Journal of the Korean Society for Precision Engineering
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    • v.17 no.8
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    • pp.100-105
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    • 2000
  • In this study the structural design concepts of main parameters of a Cathode Ray Tube(CRT) such as frame spring and shadow mask were proposed to guarantee a failure-proof CRT under mechanical shock. With computer simulation and experiments some information on the structural design concept was obtained as followings: the frame and the shadow mask of the CRT needed designing to increase strength so double-beads shape at the corner of frame was newly designed for it, And the spring which interconnected frame with panel glass was required to deform elastically for the purpose of absorbing the shock energy in the direction of drop. A new type of spring 'twisting spring' was designed to achieve the flexibility in that direction. By using it the deformation energy of a shadow mask could reduced to some degree. To accomplish those simulations commerical codes Pam-Crash and I-DEAS were used and a typical CRT was analyzed as an example to prove the usefulness of this study.

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A study of the plan dosimetic evaluation on the rectal cancer treatment (직장암 치료 시 치료계획에 따른 선량평가 연구)

  • Jeong, Hyun Hak;An, Beom Seok;Kim, Dae Il;Lee, Yang Hoon;Lee, Je hee
    • The Journal of Korean Society for Radiation Therapy
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    • v.28 no.2
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    • pp.171-178
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    • 2016
  • Purpose : In order to minimize the dose of femoral head as an appropriate treatment plan for rectal cancer radiation therapy, we compare and evaluate the usefulness of 3-field 3D conformal radiation therapy(below 3fCRT), which is a universal treatment method, and 5-field 3D conformal radiation therapy(below 5fCRT), and Volumetric Modulated Arc Therapy (VMAT). Materials and Methods : The 10 cases of rectal cancer that treated with 21EX were enrolled. Those cases were planned by Eclipse(Ver. 10.0.42, Varian, USA), PRO3(Progressive Resolution Optimizer 10.0.28) and AAA(Anisotropic Analytic Algorithm Ver. 10.0.28). 3fCRT and 5fCRT plan has $0^{\circ}$, $270^{\circ}$, $90^{\circ}$ and $0^{\circ}$, $95^{\circ}$, $45^{\circ}$, $315^{\circ}$, $265^{\circ}$ gantry angle, respectively. VMAT plan parameters consisted of 15MV coplanar $360^{\circ}$ 1 arac. Treatment prescription was employed delivering 54Gy to recum in 30 fractions. To minimize the dose difference that shows up randomly on optimizing, VMAT plans were optimized and calculated twice, and normalized to the target V100%=95%. The indexes of evaluation are D of Both femoral head and aceta fossa, total MU, H.I.(Homogeneity index) and C.I.(Conformity index) of the PTV. All VMAT plans were verified by gamma test with portal dosimetry using EPID. Results : D of Rt. femoral head was 53.08 Gy, 50.27 Gy, and 30.92 Gy, respectively, in the order of 3fCRT, 5fCRT, and VMAT treatment plan. Likewise, Lt. Femoral head showed average 53.68 Gy, 51.01 Gy and 29.23 Gy in the same order. D of Rt. aceta fossa was 54.86 Gy, 52.40 Gy, 30.37 Gy, respectively, in the order of 3fCRT, 5fCRT, and VMAT treatment plan. Likewise, Lt. Femoral head showed average 53.68 Gy, 51.01 Gy and 29.23 Gy in the same order. The maximum dose of both femoral head and aceta fossa was higher in the order of 3fCRT, 5fCRT, and VMAT treatment plan. C.I. showed the lowest VMAT treatment plan with an average of 1.64, 1.48, and 0.99 in the order of 3fCRT, 5fCRT, and VMAT treatment plan. There was no significant difference on H.I. of the PTV among three plans. Total MU showed that the VMAT treatment plan used 124.4MU and 299MU more than the 3fCRT and 5fCRT treatment plan, respectively. IMRT verification gamma test results for the VMAT plan passed over 90.0% at 2mm/2%. Conclusion : In rectal cancer treatment, the VMAT plan was shown to be advantageous in most of the evaluation indexes compared to the 3D plan, and the dose of the femoral head was greatly reduced. However, because of practical limitations there may be a case where it is difficult to select a VMAT treatment plan. 5fCRT has the advantage of reducing the dose of the femoral head as compared to the existing 3fCRT, without regard to additional problems. Therefore, not only would it extend survival time but the quality of life in general, if hospitals improved radiation therapy efficiency by selecting the treatment plan in accordance with the hospital's situation.

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New analysis method of electrostatic lens for CRT

  • Seok, J.M.;Ham, Y.S.;Lee, J.I.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2002.08a
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    • pp.395-398
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    • 2002
  • The spherical aberration and optical integer (f) of the electron gun's main lens in color CRT is obtained, using electron beam trajectory. A spherical aberration is obtained from the relation between the object plane and the image of a beam trajectory. To analyze beam profile, 3rd and 1st order coefficient were obtained and used. It is shown that, in practice, they are applied to electron gun design.

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Monitoring of Environmental Arsenic by Cultures of the Photosynthetic Bacterial Sensor Illuminated with a Near-Infrared Light Emitting Diode Array

  • Maeda, Isamu;Sakurai, Hirokazu;Yoshida, Kazuyuki;Siddiki, Mohammad Shohel Rana;Shimizu, Tokuo;Fukami, Motohiro;Ueda, Shunsaku
    • Journal of Microbiology and Biotechnology
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    • v.21 no.12
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    • pp.1306-1311
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    • 2011
  • Recombinant Rhodopseudomonas palustris, harboring the carotenoid-metabolizing gene crtI (CrtIBS), and whose color changes from greenish yellow to red in response to inorganic As(III), was cultured in transparent microplate wells illuminated with a light emitting diode (LED) array. The cells were seen to grow better under near-infrared light, when compared with cells illuminated with blue or green LEDs. The absorbance ratio of 525 to 425 nm after cultivation for 24 h, which reflects red carotenoid accumulation, increased with an increase in As(III) concentrations. The detection limit of cultures illuminated with near-infrared LED was 5 ${\mu}g$/l, which was equivalent to that of cultures in test tubes illuminated with an incandescent lamp. A near-infrared LED array, in combination with a microplate, enabled the simultaneous handling of multiple cultures, including CrtIBS and a control strain, for normalization by the illumination of those with equal photon flux densities. Thus, the introduction of a near-infrared LED array to the assay is advantageous for the monitoring of arsenic in natural water samples that may contain a number of unknown factors and, therefore, need normalization of the reporter event.

Functional Characterization of sll1556 of Synechocystis sp. PCC6803 as Type II Isopentenyl Diphosphate Isomerase (Type II Isopentenyl Diphosphate Isomerase로서 Synechocystis sp. PCC6803의 sll1556의 작용 특성)

  • Cho, Kab-Yeon
    • The Korean Journal of Food And Nutrition
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    • v.23 no.4
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    • pp.526-530
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    • 2010
  • Isopentenyl diphosphate(IPP) isomerization to dimethylallyl diphosphate(DMAPP) is an important step for the efficient production of isoprenoids such as lycopene, ${\beta}$-carotene, astaxanthin, etc. The type II isopentenyl diphosphate isomerase gene from Synechocystis sp. PCC6803(sll1556, Syidi2) was cloned and expressed in Escherichia coli $DH5{\alpha}$. When E. coli $DH5{\alpha}$ harboring lycopene synthesis genes, crtE, crtB, and crtI and mevalonate pathway genes, MvK1, MvK2, and Mvd, was cultured on LB medium containing mevalonate, the strain grew very slowly be due to the toxicity of isopentenyl diphosphate derived from mevalonate. When Syidi2 was introduced to E. coli $DH5{\alpha}$ harboring the lycopene synthesis genes and mevalonate pathway genes, growth on mevalonate medium was fully restored and the colony showed red color indicating lycopene formation. The growth rate of the mutant strain, E. coli $DH5{\alpha}$(idi::${\Delta}km$), was very slow because of IPP accumulation and DMAPP deprivation. Ultimately the idi mutant was complemented by introducing the Syidi2 gene.

Implementation of Biological Information System Using Microprocessor(I)-Scanconverter- (마이크로프로세서를 이용한 생체정보시스템의 구성에 관한 연구(I)-환자감시 장치용 Scanconverter를 중심으로)

  • 박상희;김원기
    • Journal of Biomedical Engineering Research
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    • v.7 no.1
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    • pp.3-10
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    • 1986
  • In this paper, a scanconverter which displays the biological signals of low frequency on CRT for the purpose of easy read-out is attempted to design for using the CRTC, and it stows some good effectivenesses The results obtained in this experiment are as follows : (1) Using only one chip, CRT controller, it can display both waveforms and characters simultaneously. (2) The flexibility of CPU program can be obtained using the sub-microproce ssor function of CRTC chip. (3) The trend of digital data is possible through the graphic function (4) The vibration of displayed waveform can be prevented using the chip simplification and the trigger signal of one chip. (5) Operation with microprocessor malies the expansion and interface easy.

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