• Title/Summary/Keyword: crtI

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Metabolic engineering of Lilium ${\times}$ formolongi using multiple genes of the carotenoid biosynthesis pathway

  • Azadi, Pejman;Otang, Ntui Valentaine;Chin, Dong Poh;Nakamura, Ikuo;Fujisawa, Masaki;Harada, Hisashi;Misawa, Norihiko;Mii, Masahiro
    • Plant Biotechnology Reports
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    • v.4 no.4
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    • pp.269-280
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    • 2010
  • Lilium ${\times}$ formolongi was genetically engineered by Agrobacterium-mediated transformation with the plasmid pCrtZW-N8idi-crtEBIY, which contains seven enzyme genes under the regulation of the CaMV 35S promoter. In the transformants, ketocarotenoids were detected in both calli and leaves, which showed a strong orange color. In transgenic calli, the total amount of carotenoids [133.3 ${\mu}g/g$ fresh weight (FW)] was 26.1-fold higher than in wild-type calli. The chlorophyll content and photosynthetic efficiency in transgenic orange plantlets were significantly lowered; however, after several months of subculture, they had turned into plantlets with green leaves that showed significant increases in chlorophyll and photosynthetic efficiency. The total carotenoid contents in leaves of transgenic orange and green plantlets were quantified at 102.9 and 135.2 ${\mu}g/g$ FW, respectively, corresponding to 5.6- and 7.4-fold increases over the levels in the wild-type. Ketocarotenoids such as echinenone, canthaxanthin, 3'-hydroxyechinenone, 3-hydroxyechinenone, and astaxanthin were detected in both transgenic calli and orange leaves. A significant change in the type and composition of ketocarotenoids was observed during the transition from orange transgenic plantlets to green plantlets. Although 3'-hydroxyechinenone, 3-hydroxyechinenone, astaxanthin, and adonirubin were absent, and echinenone and canthaxanthin were present at lower levels, interestingly, the upregulation of carotenoid biosynthesis led to an increase in the total carotenoid concentration (+31.4%) in leaves of the transgenic green plantlets.

Implementation of RSA modular exponentiator using Division Chain (나눗셈 체인을 이용한 RSA 모듈로 멱승기의 구현)

  • 김성두;정용진
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.12 no.2
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    • pp.21-34
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    • 2002
  • In this paper we propos a new hardware architecture of modular exponentiation using a division chain method which has been proposed in (2). Modular exponentiation using the division chain is performed by receding an exponent E as a mixed form of multiplication and addition with divisors d=2 or $d=2^I +1$ and respective remainders r. This calculates the modular exponentiation in about $1.4log_2$E multiplications on average which is much less iterations than $2log_2$E of conventional Binary Method. We designed a linear systolic array multiplier with pipelining and used a horizontal projection on its data dependence graph. So, for k-bit key, two k-bit data frames can be inputted simultaneously and two modular multipliers, each consisting of k/2+3 PE(Processing Element)s, can operate in parallel to accomplish 100% throughput. We propose a new encoding scheme to represent divisors and remainders of the division chain to keep regularity of the data path. When it is synthesized to ASIC using Samsung 0.5 um CMOS standard cell library, the critical path delay is 4.24ns, and resulting performance is estimated to be abort 140 Kbps for a 1024-bit data frame at 200Mhz clock In decryption process, the speed can be enhanced to 560kbps by using CRT(Chinese Remainder Theorem). Futhermore, to satisfy real time requirements we can choose small public exponent E, such as 3,17 or $2^{16} +1$, in encryption and verification process. in which case the performance can reach 7.3Mbps.

A NDT System using the Pulse-echo Method (펄스 반사법을 이용한 NDT 시스템)

  • Vhan, C.S.;Kim, K..;Cha, I.W.;Yim, K.H.
    • The Journal of the Acoustical Society of Korea
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    • v.5 no.1
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    • pp.35-40
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    • 1986
  • 본 연구는 펄스 반사법을 이용하여 금속 재료 단면의 영상을 얻는데 그 목적이 있다. 기존의 탐 상기는 대부분 A - 스캔 방식으로 결과를 전시하여 검사자가 직접 CRT 상에 나타난 결과를 판독해야 하므로 숙련자라 하더라도 어느정도 오차가 수반되며 판독에 시간이 요하는 등 불편이 따른다. 본 연구 에서는 판독 과정을 자동화함으로써 정확도와 검사 속도를 개선하였으며 탐촉 지점은 스텝핑모터를 이 용하여 일정 간격으로 제어하였다. 도 탐상 결과를 B-스캔 방식으로 영상 처리함으로써 재료 단면의 상 태 파악을 용이하게 하였다.

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The Efficient Production on single- and Multi- Wall Carbon Nanotubes

  • Shinohara, H.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2000.02a
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    • pp.207-207
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    • 2000
  • Multi- and single-wall carbon nanotubes are promising new carbon materials in nano-electronics, field-emitters, CRT-displays, hydrogen storage materials, biomedical tracers and so forth. The present talk will deal with a high-yield synthesis on quasi-aligned multi-wall carbon nanotubes via a chemical vapor deposition technique. I will also talk about a possible growth mechanism on single-wall carbon nanotubes based on newly obtained experimental results.

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The Optimization Of SS-Type Deflection Yoke By Using Genetic Algorithm (유전 알고리즘을 이용한 SS형 편향코일의 형상 최적화)

  • Joo, K.J.;Yoon, I.G.;Kang, B.H.;Joe, M.C.;Hahn, S.Y.;Lee, H.B.
    • Proceedings of the KIEE Conference
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    • 1993.07b
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    • pp.971-973
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    • 1993
  • Deflection Yoke(the following, DY) is the important electric device of CRT which deflects R, G, B beans influencing magnetic field produced by yoke coils. Recently, DY is designed to the saddle/saddle type of coils, being proposed for high-definite and high-efficient CRT. This paper presents the optimization of pin-sectioned saddle coil's shape for minimizing gap between desired and practical deflections of electron beams by using Genetic Algorithm. Evolution Startegy is utilized in this paper, since evolution strategy is a kind of genetic algorithms finding the optimized values by choicing the better generation with comparing the parents and their children. Here, the children are generated by only mutations from the normal random variables. Evolution strategy has shown better powerful converge rate than the other genetic algorithms becuase of using only the mutation-operator.

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Benefit of volumetric-modulated arc therapy over three-dimensional conformal radiotherapy for stage I-II extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue in the stomach: a dosimetric comparison

  • Chung, Joo-Hyun;Na, Kyoungsu;Kim, Il Han
    • Radiation Oncology Journal
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    • v.36 no.4
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    • pp.332-340
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    • 2018
  • Purpose: To retrospectively analyze dosimetric parameters of volumetric-modulated arc therapy (VMAT) and three-dimensional conformal radiotherapy (3D-CRT) delivered to extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue in the stomach (gastric MALT lymphoma) to find out advantages of VMAT and conditions for definite benefits of VMAT. Materials and Methods: Fifty patients with stage I-II gastric MALT lymphoma received VMAT (n = 14) or 3D-CRT (n = 36) between December 2005 and April 2018. Twenty-seven patients were categorized according to whether the planning target volume (PTV) overlaps kidney(s). Dosimetric parameters were analyzed by dose-volume histogram. Results: Radiation dose to the liver was definitely lower with VMAT in terms of mean dose (p = 0.026) and V15 (p = 0.008). The V15 of the left kidney was lower with VMAT (p = 0.065). For those with PTV overlapping kidney(s), the left kidney V15 was significantly lower with VMAT. Furthermore, the closer the distance between the PTV and kidneys, the less the left kidney V15 with VMAT (p = 0.037). Delineation of kidney(s) by integrating all respiratory phases had no additional benefit. Conclusions: VMAT significantly increased monitor units, reduced treatment time and radiation dose to the liver and kidneys. The benefit of VMAT was definite in reducing the left kidney V15, especially in geometrically challenging conditions of overlap or close separation between PTV and kidney(s).

A Gene-based dCAPS Marker for Selecting old-gold-crimson (ogc) Fruit Color Mutation in Tomato (토마토 과색 돌연변이 유전자(old-gold-crimson) 선발을 위한 dCAPS 분자표지 개발)

  • Park, Young-Hoon;Lee, Yong-Jae;Kang, Jum-Soon;Choi, Young-Whan;Son, Beung-Gu
    • Journal of Life Science
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    • v.19 no.1
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    • pp.152-155
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    • 2009
  • The old-gold-crimson ($og^c$) fruit color mutation produces deep red tomato fruit with high lycopene content. age is a null mutation allele of lycopene-${\beta}$-cyclase (Crt-b) gene (B locus) that converts lycopene to ${\beta}$-carotene in the cartenoid biosynthesis pathway in tomato. Breeding of high lycopene tomato cultivars can be accelerated by marker-assisted selection (MAS) for introgression of $og^c$ allele by using a gene-based DNA marker. In order to develop a marker, single nucleotide deletion of adenine(A) with. in a poly-A repeat that has been known to be responsible for frame-shift mutation of $og^c$ was confirmed by resequencing mutant allele and wild-type allele at B locus of several tomato lines. For allele discrimination and detection of $og^c$, derived CAPS (dCAPS) approach was used by designing a primer that artificially introduced restriction enzyme recognition site of Hin fI in PCR products from $og^c$ allele. This dCAPS marker is co-dominant gene-based PCR marker that can be efficiently used for MAS breeding program aiming the development of high lycopene tomato.

A Study on the Maskless Plate Making Technology for Screen Printing(I) (Maskless용 스크린 제판 기술 연구(I))

  • Lee, Mi-Young;Park, Kyoung-Jin;Nam, Su-Yong
    • Journal of the Korean Graphic Arts Communication Society
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    • v.26 no.1
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    • pp.73-85
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    • 2008
  • We have manufactured a photoresist which has excellent dispersity and good applying property due to 330cps of viscosity for environment-friendly and economical maskless screen plate making. And the photoresist applied on the screen stretched was exposed without mask by beam projector with CRT light source. Then it was developed by air spray with $1.7kgf/cm^2$ of injection pressure. The pencil hardness and solvent resistance of curing photoresist film were worse than those of conventional photoresist film and the maximum resolution of line image formed by maskless screen plate making was 0.5 mm since the exposure system for maskless plate making has weak light intensity and the diffusion of light. But we could obtain maskless screen plate which has sharp edges of line image and confirm a possibility of dry development process by air spray method.

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Automation of Lumber Drying System(I) -Continuously Rising Temperature Drying of Pinus densiflora- (목재건조(木材乾燥)의 자동화(自動化)에 관한 연구(硏究)(I) -연속온도상승(連續溫度上昇)스케쥴을 이용한 목재건조장치(木材乾燥裝置) 자동화(自動化)-)

  • Lee, Hyoung-Woo
    • Journal of the Korean Wood Science and Technology
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    • v.22 no.1
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    • pp.12-19
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    • 1994
  • An electrically heated experimental lumber dry kiln was retrofitted with a computer-based control system to control kiln conditions more precisely and monitor and record several kiln variables. Flat-sawn 2.5cm-thick Pinus densiflora boards were dried in constant temperature process(65$^{\circ}C$ & 50~60 %RH) and continuously rising temperature process, respectively. The average drying rate in continuously rising temperature process was 5.7 %/hr, which was above 3 times faster than that in constant temperature process. But, the average rate of case-hardening and moisture difference between shells and cores of boards dried in continuously rising temperature process were 82 % and 5.5 %, respectively, which were much larger than those of boards dried in constant temperature process.

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