• Title/Summary/Keyword: crossing breeding

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Estimation of Genetic Parameters and Reproductivity Test of Genetic Evaluation for Growth-related Traits of olive Flounder Paralichthys olivaceus at 180 Days of Age (180일령 넙치 Paralichthys oilvaceus의 성장형질에 대한 유전모수 추정 및 유전능력평가 재현성 검정)

  • Kim, Hyun-Chul;Noh, Jae-Koo;Lee, Jeong-Ho;Kim, Jong-Hyun;Park, Choul-Ji;Kang, Jung-Ha;Kim, Kyung-Kil;Lee, Jung-Gyu;Myeong, Jeong-In
    • Journal of Aquaculture
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    • v.21 no.4
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    • pp.317-324
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    • 2008
  • This study estimated the genetic parameters and breeding values for the growth-related traits of the 1st generation produced in 2005. The heritability of body weight, total length, body height, body shape and condition factor of 180 days old olive flounders Paralichthys olivaceus, the 1st generation of selection, was estimated as 0.564, 0.590, 0.588, 0.306 and 0.332, respectively. And reproductivity of genetic evaluation for crossing superior flounders and inferior ones was tested using the subsequent generation produced in 2006 based on the breeding values of 1st generation. In the least-squares means of body weight and total length for each group of crossing, the values of crossing group between superior flounders ($S{\times}S$) showed $145.6{\pm}1.8\;g$ and $22.4{\pm}0.1\;cm$, respectively. The values of crossing group between superior and inferior flounders ($S{\times}I$) showed $133.2{\pm}2.5\;g$ and $22.1{\pm}0.1\;cm$, respectively. The values of crossing group between inferior flounders ($I{\times}I$) showed $114.0{\pm}2.1\;g$ and $21.08{\pm}0.12\;cm$, respectively. In the results, flounders are determined as appropriate selective breeding fish with the high heritability of flounders in early ages at 180 days old, and the reproductivity of genetic evaluation was also high.

Potential Benefit of Genetic Engineering in Plant Breeding: Rice, a Case Study

  • Datta, Swapan K.
    • Journal of Applied Biological Chemistry
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    • v.43 no.4
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    • pp.197-206
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    • 2000
  • This paper summarizes recent developments in the field of molecular biology and its application to plant breeding, particularly in rice. Plant breeding in the past mostly depended on the time-consuming crossing of known genomes limited to certain traits. Plant breeding has now benefited from marker-assisted selection and genetic engineering to widen the gene pool, improve plant protection, and increase yield. Future plant breeding will expand based on functional and nutritional genomics, in which gene discovery and high-throughput transformation will accelerate crop design and benefits will accrue to human health, in the form of nutritional food for poor people to reduce malnutrition, or food enriched with antioxidants and with high food value for rich people. Agricultural biotechnology for food is no longer a dream but a reality that will dominate the 21st century for agriculture and human welfare.

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Breeding of Productive Bivoltine Hybrid, CSR16${\times}$CSR17 of Silkworm Bombyx mori L.

  • Reddy, N.Mal;Basavaraja, H.K.;Kumar, N.Suresh;Joge, P.G.;Kalpana, G.V.;Dandin, S.B.;Datta, R.K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.2
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    • pp.129-133
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    • 2004
  • The breeding work was initiated by utilizing two Japanese hybrids namely C135${\times}$N134 and N137${\times}$C146 along with two evolved Indian breeds, J14 and A24. The breed CSR16 which is characterized with marked larvae and white dumbbell cocoons was evolved from the Japanese hybrid C135${\times}$N134 by crossing with J14, while the breed CSR17 which is characterized with plain larvae and white oval cocoons was evolved from the Japanese hybrid N137${\times}$C146 by crossing with A24. The hybrid of these evolved breeds i.e., CSR16${\times}$CSR17 has shown superior over control hybrid $KA{\times}{NB_4}{D_2}$ and on par with the ruling hybrid of CSR2${\times}$CSR4. The hybrid CSR16${\times}$CSR17 was authorized during 1999 by Central Silk Board, Bangalore, Government of India for commercial exploitation during favourable months based on national level race authorization test.

Cross-breeding of Neopyropia spp. (Bangiales, Rhodophyta) Using CAPS (Cleaved Amplified Polymorphic Sequence) Markers (CAPS (Cleaved Amplified Polymorphic Sequence) 마커를 적용한 김 교잡육종 기술 개발)

  • Eun-Jeong Park
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.1
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    • pp.124-132
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    • 2023
  • This study aimed to cross between Korean and Japanese pure lines of Neopyropia strains to establish cross breeding technology and identify a superior variety that harbors the strength of both parents. Four crossing combinations were tried using three methods, resulting in 1,476 single conchocelis colonies. The three co-dominant Cleaved Amplified Polymorphic Sequence (CAPS) markers (EF-1α/Mse I, TOP2/Mse I, car A/ApaL I) were used to distinguish heterozygotic sporophytes and their maternal lines obtained from the inter and intraspecific cross-fertilization within the wild type of Neopyropia strains. Of the 1,476 colonies, 26.9% (218) were heterozygotes obtained from the nuclear CAPS markers. Their maternal line was clearly confirmed using organelle CAPS marker and chimeric thallus was obtained from crossing experiment of Japanese N. yezoensis (♀) and Korean N. yezoensis (♂). The use of CAPS markers improved the efficiency of crossbreeding by quickly screening heterozygotes and maternal lines in the conchocelis phase, which otherwise required pigmentation mutants as genetic markers.

Influence of roadkill during breeding migration on the sex ratio of land crab (Sesarma haematoche)

  • Ryu, Mi;Kim, Jae Geun
    • Journal of Ecology and Environment
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    • v.44 no.4
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    • pp.207-211
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    • 2020
  • Adult land crabs generally live on land while their larvae live in the sea. In the case of Sesarma haematoche, female crabs migrate from land to sea to release the larvae at the high tide of syzygy night. Artificial structures along coastal areas are being obstacles for the migration of land crabs and causing synchronized roadkills on coastal roads during breeding migration. In this research, we compared the sex ratios of crab populations in coastal areas with coastal roads and uninhabited island areas with no road. The proportion of females in inland habitats with coastal roads was significantly smaller than island habitats. In particular, females are exposed to the risk of annually repeated roadkills, and the proportion of females decreases rapidly with their growth. If this tendency is general for land crab populations in the coastal areas with roads, significant road mortality of female land crabs during breeding migration can lead to severe population decline in coastal areas. Therefore, it is necessary to take an action to save land crabs crossing coastal roads.

A New Spray Rose Cultivar, "Yellow King" with Yellow Color and Resistance to the Powdery Mildew (흰가루병에 강한 황색 스프레이 장미 품종 "옐로킹" 육성)

  • Kim, Jin-Ki;An, Dong-Choon;Kim, Su-Kyeong;Been, Chul-Gu;Kim, Zhoo-Hyeon
    • Korean Journal of Breeding Science
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    • v.40 no.1
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    • pp.54-57
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    • 2008
  • The New bicolor spray rose (Rose hybrida Hort.) cultivar was developed by line selection in 2000 at the Flower Breeding Research Institute, Gyeongnam ARES. Characteristics were investigated three times from 2003 to 2006. 'Yellow King' was crossed between 'Spring Time' and 'Flair' and it has a Yellow Orange-group color (RHS No. 16A), few thorns and spray flower with a good harmony between ray floret and flower center. The vase life of cut flower was 9.1days. It was registered as commercial cultivar in 2006. This new cultivar could be planted in most of greenhouse production region of Korea and will be released to general rose growers in 2007.

Genetic Diversity of Korean Rice Breeding Parents as Measured by DNA Fingerprinting with Simple Sequence Repeat (SSR) Markers

  • Song, Moon-Tae;Lee, Jeom-Ho;Lee, Sang-Bok;Cho, Youn-Sang;Ku, Ja-hwan;Seo, Kyoung-In;Choi, Seong-ho;Hwang, Heung-Goo
    • Plant Resources
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    • v.6 no.1
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    • pp.16-26
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    • 2003
  • Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. Present study was conducted to evaluate the genetic diversity within a diverse collection of rice accessions used for Korean breeding programs. Two hundred eighty-seven rice cultivars, composed of temperate japonica, tropical japonica, indica, and Tongil-type of Korean crossing parents were evaluated by means of 15 simple sequence repeat (SSR) markers. A total of 99 alleles were detected, and the number of alleles per marker ranged from 4 to 11, with an average of 6.6 per locus. Polymorphism information content (PIC) for each of the SSR markers ranged from 0.2924 to 0.8102 with an average of 0.5785. These results, with the result that use of only 15 SSR markers made all rice cultivars examined could be uniquely distinguished, imply the efficiency of SSR markers for analysis of genetic diversity in rice. Cluster analysis was performed on similar coefficient matrics calculated from SSR markers to generate a dendogram in which two major groups corresponding to japonica (Group I) and indica and Tongil type rice (group II) with additional subclasses within both major groups. The narrowness of the Korean breeding germplasm was revealed by the fact that most of the Korean-bred and Japan-bred temperate japonica cultivars were concentrated into only 2 of the sub-group I-1 (143 cultivars) and I-2 (58 cultivars) among six sub-groups in major group of japonica. This is because of the japonica accessions used in this study was a very closely related ones because of frequent sharing of the crossing parents with similar genetic background with synergy effect of the inherited genetic difference between indica and japonica. A rice breeding strategy with the use of molecular markers was discussed for overcoming of genetic vulnerability owing to this genetic narrowness.

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Genetic Structure of the Mulberry Silkworm Population in Sri Lanka: I. Estimation of Combining Ability and Heritability

  • Lea, Ho-Zoo;Alwis, Siriani-M.de
    • Journal of Sericultural and Entomological Science
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    • v.37 no.1
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    • pp.10-15
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    • 1995
  • Genetic characterization of Sri Lankan silkworm bivoltine population has not been at-tempted so far, since its sporadic introduction of bivoltine strains into the island, starting from the 1950's. Genetic structure of Sri Lankan population of mulberry silkworm Bombyx mori was investigated through estimation of general (GCA) and specific combining ability(SCA) and heritability(${h^2}_B$), on the economic quantitative characters from leading 8 inbreds and their 28 F1's in a half diallel cross, in an attempt to utilize the estimates in determination of future breeding methods and to predict the breeding value over the phenotypic value. It was found that the breeding population of the bivoltine silkworm in Sri Lanka has still maintained considerable amounts of additive gene action as well as nonadditive. For some time in the future, both breeding strategies of "selection without inbreeding" and also "inbreeding followed by crossing" should therefore be effective in genetic improvement of economic characters investigated. In addition, superior combiners in general and in specific F1′s were identified for each of 6 economic characters, to be immediately utilized in selection and also in cross breeding programs in Sri Lanka.

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'Honghwa' an Asiatic Hybrid Lily with Unspotted Orange Petals

  • Rhee, Hye Kyung;Lim, Jin Hee;Cho, Hae Ryong;Joung, Hyang Young
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.537-540
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    • 2008
  • 'Honghwa' an Asiatic hybrid lily was released in 2001 at National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), and Suwon, Korea. The cross was made in 1994 between Lilium Asiatic 'Avignon', a scarlet red colored, and L. Asiatic 'Connecticut King', bright yellow colored. It was preliminarily selected as 'A96-3' in 1996. Multiplication and bulbing, and characteristic tests were performed from 1997 to 2000. The evaluation of characteristics was made investigated as 'Wongyo C1-31' in 2001 at Suwon. 'Honghwa' flowers at the beginning of June and grows to 111.4 cm stem length. Flowers are upward-facing, unspotted with orange petals. Year-round flowering is possible by storing the bulb at $-1.5^{\circ}C$ conditions. It is necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. It is needed to control Botrytis disease during wet season.

A New Variety, 'Yeeun', an Asiatic Hybrid Lily for Pot and Bedding Plant

  • Rhee, Hye-Kyung;Lim, Jin Hee;Cho, Hae Ryong;Kim, Mi Seon;Shin, Hak Ki
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.546-549
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    • 2008
  • 'Yeeun' an Asiatic hybrid lily was released in 2005 at National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), Suwon, Korea. The cross was made in 1994 between Liliium Asiatic 'White Bird' white colored, and Liliium Asiatic 'Cote d'Azur', pink colored variety. It was preliminarily selected as 'A96-5' in 1996. Multiplication and bulbing, and characteristic tests were conducted from 1997 to 2004. The evaluation of characteristics was investigated as 'Wongyo C1-21' in 2004 at Suwon. 'Yeeun' flowers in the end of June and grows 41.6 cm stem length. Flowers are upward-facing, unspotted in petals and yellow green (RHS, 18C). Year-round flowering can be by storing the bulb under $-1.5^{\circ}C$ conditions. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. It is needed to control Botrytis disease in wet season.