• 대한미생물학회지
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• 제22권2호
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• pp.117-123
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• 1987

Previous studies have been performed for the sero-identification of selected species of Bacteroides by immunofluorescence antibody techniques and enzyme-linked immunosorbent assay using species-specific rabbit antisera to B. gingivalis, B. intermedius, and B. melaninogenicus. However, these studies have not commented on the serological cross-reactivity between these 3 species of black- pigmented Bacteroides. For the cross-reactivity study, antisera to B. gingivalis ATCC 33277, B. intermedius ATC C25261 and B. asaccharolyticus ATCC 25260 were raised from rabbits. Preliminary study for observing the cross-reactivity between these species was performed by indirect immunoflourescence technique. Immunoabsorption of the antisera was done with bacterial cells from the other species and the species-specificity of the antisera was conformed by the absence of reactivity with bacterial strains from the other species by indirect immunofluorescence technique and enzyme-linked immunosorbent assay. Three representative unabsorbed antisera cross-reacted strongly with cells from the other species. Especially, anti-B. asaccharolyticus ATCC 25260 antiserum showed a strong cross-reactivity with B. gingivalis ATCC33277. After immunoabsorption of 3 representative antisera with the other species, the cross-reactivity was found only between B. gingivalis ATCC 33277 and B. asaccharolyticus ATCC 25260. Further study would be necessary to clarify the cross-reactivity between important oral black-pigmented Bacteroides from subgingival plaque or bacterial colonies for rapid identification.

• Korean Journal of Radiology
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• 제1권3호
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• pp.127-134
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• 2000

Objective: To determine the extent to which thin-section and volumetric three-dimensional CT can depict airway reactivity to bronchostimulator, and to assess the effect of different airway sizes on the degree of reactivity. Materials and Methods: In eight dogs, thin-section CT scans were obtained before and after the administration of methacholine and ventolin. Cross-sectional areas of bronchi at multiple levels, as shown by axial CT, proximal airway volume as revealed by three-dimensional imaging, and peak airway pressure were measured. The significance of airway change induced by methacholine and ventolin, expressed by percentage changes in cross-sectional area, proximal airway volume, and peak airway pressure was statistically evaluated, as was correlation between the degree of airway reactivity and the area of airways. Results: Cross-sectional areas of the bronchi decreased significantly after the administration of methacholine, and scans obtained after a delay of 5 minutes showed that normalization was insufficient. Ventolin induced a significant increase in cross-sectional areas and an increase in proximal airway volume, while the effect of methacholine on the latter was the opposite. Peak airway pressure increased after the administration of methacholine, and after a 5-minute delay its level was near that of the control state. Ventolin, however, induced no significant decrease. The degree of airway reactivity did not correlate with airway size. Conclusion: Thin-section and volumetric spiral CT with three-dimensional reconstruction can demonstrate airway reactivity to bronchostimulator. The degree of reactivity did not correlate with airway size.

• 약학회지
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• 제48권1호
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• pp.47-54
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• 2004

ClpP is a stress-inducible protein and proteolytic subunit of the ATP-dependent Clp protease in prokaryotes and eukaryotes. Although its physiological roles in bacterial virulence were widely studied in various organsims, including Streptococcus pneumoniae, until now the immunological effect has not been investigated. Here, we have examined the cross reactivity of S. pneumoniae ClpP antibody with other organisms's cell lysate proteins. Although the protein sequence of S. pneumoniae ClpP was highly conserved among various organisms including human, the antibody rasised by S. pneumoniae ClpP was not cross-reacted with other organism's cell lysates, which were Saccharomyces cerevisiae , human lung A549 cell, Bacillus subtilis, Pseuomonas aeruginosa, E. coli, and Salmonella typhi. It was only reacted with S. pneumoniae and Lato-bacillus thermophilus. Thus we examined the immunoprotective effect of ClpP by immunizing mice with the purified ClpP. The mean survival time of mouse was significantly increased with the ClpP immunization. These results suggest that S. pneumoniae ClpP could be used as a vaccine candidate for prevention of S. pneumoniae infection.

• Nuclear Engineering and Technology
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• 제17권2호
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• pp.98-104
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• 1985

가압중수로인 CANDU의 노심에는 많은 반응도 조절장치들이 분포되어 있어 출력분포와 잉여반응도를 조절하며, 이러한 장치들의 효자는 노심해서에서 증분격자상수로 나타낸다. 격자코드인 WIMS를 사용하여 2군 군정수를 계산하고 이를 이용하여 SUPERCELL코드로 증분 격자상수를 생산하였다. 증분격자상수는 조정봉과 지역조절장치에 대해 노심해석을 통해 평가하였으며 반응도가와 채널출력을 참고 자료와 비교하였다. 반응도가와 최대채널출력 오차가 참고값에 대해 각각 0.97%와 0.6% 범위 내에서 나타났다.

• Parasites, Hosts and Diseases
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• 제40권4호
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• pp.195-198
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• 2002

This study was performed to investigate whethere there is cross-reactivity between Dirofilaria immitis and three intestinal nematodes of dogs. In ELISA, D. immitis- infected dog sera obtained at the 4th molting stage (9-11 weeks) and microfilaremic stage (25-30 weeks) were shown to be highly reactive with crude extract of T. canis. In immunoblotting, some antigenic fractions, 44, 57 88, 100 kDa of crude extract of T. canis, were found to be positive reaction with sera of dogs infected with D. immitis. However, little or no cross-reaction were observed between sera of D. immitis-infected dogs and crude extract antigen of T. vulpis or A. caninum. These result suggest that there are partial cross reaction between sera of D. immitis-infected dogs and the antigen of T. canis.

• Nuclear Engineering and Technology
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• 제55권5호
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• pp.1775-1782
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• 2023

Several reactor physics commissioning experiments were conducted to obtain the neutronic parameters at the beginning of the G.A. Siwabessy Multi-purpose Reactor (RSG-GAS) operation. These parameters are essential for the reactor to safety operate. Leveraging the experimental data, this study evaluated the calculated core reactivity, control rod reactivity worth, integral control rod reactivity curve, and fuel reactivity. Calculations were carried out with Serpent 2 code using the latest neutron cross-section data ENDF/B-VIII.0. The criticality calculations were carried out for the RSG-GAS first core up to the third core configuration, which has been done experimentally during these commissioning periods. The excess reactivity for the second and third cores showed a difference of 510.97 pcm and 253.23 pcm to the experiment data. The calculated integral reactivity of the control rod has an error of less than 1.0% compared to the experimental data. The calculated fuel reactivity value is consistent with the measured data, with a maximum error of 2.12%. Therefore, it can be concluded that the RSG-GAS reactor core model is in good agreement to reproduce excess reactivity, control rod worth, and fuel element reactivity.

• 약학회지
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• 제47권5호
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• pp.266-270
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• 2003

We evaluated four commercially available methamphetamine immunoassays for their relative cross-reactivities of amphetamine analogues in human urine: Abbott TDx, Vitalab Selectra and on-site test kits (Accusign MET, SD bioline MET). High cross-reactivities were shown at designer's drugs such as methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA) and methylenedioxyethylamphetamine (MDEA) in all of the tested immunoassays. Methoxyphenamine, fenfluramine and phentermine were positive in TDx and Selectra, but were not positive in on-site test kits. Pseudoephedrine, norpseudoephedrine, ephedrine, norephedrine, MDMA, MDA, fenfluramine and phentermine were detected by gas chromatography/mass spectrometry(GC/MS) in false positive urines. Since the overall specificity of any of the devices was not 100％, we found it is important to confirm any positive screening test result, so we developed simultaneous determination of amphetamine analogues in urines. After alkalinization of the urine samples with 6-N NaOH, the analytes were extracted using ethyl acetate, derivatized with pentafluoropropyl anhydride (PFPA) prior at GC/MS analysis.

• Nuclear Engineering and Technology
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• 제44권2호
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• pp.177-198
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• 2012

This paper reviews the fast reactor physics and computational methods. The basic reactor physics specific to fast spectrum reactors are briefly reviewed, focused on fissile material breeding and actinide burning. Design implications and reactivity feedback characteristics are compared between breeder and burner reactors. Some discussions are given to the distinct nuclear characteristics of fast reactors that make the assumptions employed in traditional LWR analysis methods not applicable. Reactor physics analysis codes used for the modeling of fast reactor designs in the U.S. are reviewed. This review covers cross-section generation capabilities, whole-core deterministic (diffusion and transport) and Monte Carlo calculation tools, depletion and fuel cycle analysis codes, perturbation theory codes for reactivity coefficient calculation and cross section sensitivity analysis, and uncertainty analysis codes.

• Journal of Microbiology and Biotechnology
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• 제7권3호
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• pp.194-196
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• 1997

Using the polyclonal antibody for Xanthomonas citri ampicillin acylase raised in Pseudomonas-free Balb/c mice, the immunochemical similarity of several types of penicillin acylases including Erwinia aroideae penicillin V acylase, Escherichia coli penicillin G acylase, Pseudomonas melanogenum and Acetobacter turbidans ampicillin acylases, and Pseudomonas cephalosporin acylase was examined. Among tested, only P. melanogenum ampicillin acylase showed the cross-reactivity with the antibody.

• Nuclear Engineering and Technology
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• 제49권6호
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• pp.1287-1300
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• 2017

Based on the observation that ignoring the angle dependency of multigroup resonance cross sections within a fuel pellet would result in nontrivial underestimation of the spatial self-shielding of flux, a parametrized spectral superhomogenization (SPH) factor library (PSSL) method is developed as a practical means of resolving the problem. Region-wise spectral SPH factors are calculated by the normal and transport corrected SPH iterations after ultrafine group slowing down calculations over various light water reactor pin-cell configurations. The parametrization is done with fuel temperature, U-238 number density, fuel radius, moderator source represented by ${\Sigma}_{mod}V_{mod}$, and the number density ratio of resonance nuclides to that of U-238 in a form of resonance interference correction factors. The parametrization is successful in that the root mean square errors of the interpolated SPH factors over the fuel regions of various pin-cells are within 0.1%. The improvement in reactivity error of the PSSL method is shown to be superior to that by the original SPH method in that the reactivity bias of -200 pcm to -300 pcm vanishes almost completely. It is demonstrated that the environment effect takes only about 4% in the reactivity improvement so that the pin-cell based PSSL method is effective in the assembly problems.