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Microbiota and Physicochemical Analysis on Traditional Kocho Fermentation Enhancer to Reduce Losses (Gammaa) in the Highlands of Ethiopia

  • Dibaba, Adane Hailu;Tuffa, Ashenafi Chaka;Gebremedhin, Endrias Zewdu;Nugus, Gerbaba Guta;Gebresenbet, Girma
    • Microbiology and Biotechnology Letters
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    • v.46 no.3
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    • pp.210-224
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    • 2018
  • Warqe (Ensete ventricosum) has been traditionally fermented in an earthen pit to yield a carbohydrate-rich food product named kocho, for generations. A fermentation enhancer (gammaa) was added to this fermenting mass to enhance the fermentation process. The objectives of this study were to assess the physicochemical properties and microbiota of the kocho fermentation enhancer culture to reduce losses. Cross-sectional study design was implemented to collect 131 gammaa samples on the first day of fermentation. The samples were further classified into four groups according to the duration of fermentation (14, 21, 30, and 60 days) practised in various households traditionally. The results showed that the fermentation time significantly affected the physicochemical properties and microbial load of gammaa (p < 0.01). As the fermentation progressed from day 1 to 60, the pH decreased and the titratable acidity increased. The total coliform, Enterobacteriaceae, aerobicmesophilic bacteria (AMB), yeast, and mould counts were significantly reduced at the end of fermentation. In contrast, the number of lactic acid bacteria (LAB) increased significantly until day 30 of fermentation, because of the ability of the LAB to grow at low pH. Lactobacillus species from LAB isolates and Enter obacteriaceae from AMB isolates were the most abundant microorganisms in gammaa fermentation. However, the Enterobacteriaceae and Lactobacilli species count showed decreasing and increasing trends, respectively, as the fermentation progressed. These isolates must be investigated further to identify the species and strain, so as to develop gammaa at the commercial scale.

Pharmacokinetic Evaluation of Flurbiprofen Sustained Release Capsule (플루르비프로펜 서방캅셀의 약물속도론적 평가)

  • Park, Kyoung-Ho;Lee, Min-Hwa;Yang, Min-Yeol;Lee, Chong-Won
    • Journal of Pharmaceutical Investigation
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    • v.23 no.3
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    • pp.179-186
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    • 1993
  • In vitro dissolution test and pharmacokinetic study in human volunteers were conducted to evaluate the pharmacokinetic characteristics of 150 mg furbiprofen sustained-release capsule (FPSR-150). As a reference product, 50 mg flurbiprofen conventional-release capsule (FPCR-50) was used. Dissolution tests of two products were run using the paddle method in 450 : 540 (v/v %) mixture of simulated gastric and intestinal fluids (K.P. VI) by adjusting medium pH according to time. FPCR-50 was dissolved very rapidly, and it took about 1.5 hr for FPCR-50 to be dissolved over 90%, whereas 15 hr for FPSR-150. Also, in pharmacokinetic study, ten healthy male volunteers were administered one capsule of FPSR-150 or two capsules of FPCR-50 (FPCR-l00) with randomized two period cross-over study. Significant differences between FPCR-l00 and FPSR-150 were found in mean times to reach peak concentration, mean resident times and mean terminal phase halflives, while not in AUC/Dose (Student's t-test). In ANOVA for AUC/Dose to compare the bioavailabilities of two FP products, there was no significant difference. From the comparison of the simulated steady-state plasma concentration-time curves following multiple medications of FPCR-50 (3 capsules a day, dosing interval=8 hrs) and FPSR-150 (1 capsule a day) based on the above results obtained from single doses of two FP products, it was noted that the medication of FPSR-150 is more useful in clinical application rather than FPCR-50.

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Preparation and Characterization of Salmon Patty using Muscle from Salmon Frame (연어 frame 유래 근육을 이용한 연어 패티의 제조 및 특성)

  • Heu, Min-Soo;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.3
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    • pp.183-189
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    • 2009
  • This study was conducted to prepare salmon patty using muscle separated from salmon frame (SPFM) and to investigate the food component characterization. When compared to salmon patty with fillet muscle (SPM), SPFM was lower in the moisture content, while it was higher in crude lipid content. However, no differences in the ash and protein contents between SPFM and SPM were found. Compared to SPM, the Hunter color value in cross section of cooked SPFM was higher in a and $\Delta$E values, while the color was lower in Land b values. Trichloroacetic acid soluble-N content of SPFM was 279 mg/100 g, which was insignificantly different (P>0.05) compared to those of SPM and commercial patty. The hardness of SPFM was 0.44 kg/$cm^2$, which was insignificantly different (P>0.05) compared to that of SPM, while was higher than that of commercial patty. The major fatty acids of SPFM were 16:0 (16.5%), 18:1n-9 (29.2%) and 18:2n-6 (26.1%). The 20:5n-3 and 22:6n-3 were also detected in high composition. The total amino acid content of SPFM was 16.6 g/100 g, which was similar to that of SPM. However, the total amino acid of SPFM was 14% higher than that of commercial patty. From the results of the mineral content, SPFM was higher than that of SPM in Fe and Ca, while the K in SPFM was lower. According to the result of sensory evaluation on the color, flavor and taste, no significant differences in all sensory items between SPFM and SPM were found.

Application of LATE-PCR to Detect Candida and Aspergillus Fungal Pathogens by a DNA Hybridization Assay

  • Gopal, Dhayaalini Bala;Lim, Chua Ang;Khaithir, Tzar Mohd Nizam;Santhanam, Jacinta
    • Microbiology and Biotechnology Letters
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    • v.45 no.4
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    • pp.358-364
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    • 2017
  • Asymmetric PCR preferentially amplifies one DNA strand for use in DNA hybridization studies. Linear-After-The-Exponential-PCR (LATE-PCR) is an advanced asymmetric PCR method which uses innovatively designed primers at different concentrations. This study aimed to optimise LATE-PCR parameters to produce single-stranded DNA of Candida spp. and Aspergillus spp. for detection via probe hybridisation. The internal transcribed spacer (ITS) region was used to design limiting primer and excess primer for LATE-PCR. Primer annealing and melting temperature, difference of melting temperature between limiting and excess primer and concentration of primers were optimized. In order to confirm the presence of single-stranded DNA, the LATE-PCR product was hybridised with digoxigenin labeled complementary oligonucleotide probe specific for each fungal genus and detected using anti-digoxigenin antibody by dot blotting. Important parameters that determine the production of single-stranded DNA in a LATE-PCR reaction are difference of melting temperature between the limiting and excess primer of at least $5^{\circ}C$ and primer concentration ratio of excess primer to limiting primer at 20:1. LATE-PCR products of Candida albicans, Candida parapsilosis, Candida tropicalis and Aspergillus terreus at up to 1:100 dilution and after 1 h hybridization time, successfully hybridised to respective oligonucleotide probes with no cross reactivity observed between each fungal genus probe and non-target products. For Aspergillus fumigatus, LATE-PCR products were detected at 1:10 dilution and after overnight hybridisation. These results indicate high detection sensitivity for single-stranded DNA produced by LATE-PCR. In conclusion, this advancement of PCR may be utilised to detect fungal pathogens which can aid the diagnosis of invasive fungal disease.

Numerical Investigation of Smoke Behavior in Rescue Station for Tunnel Fire (철도터널 화재 시 구난역 내의 연기거동에 대한 수치해석 연구)

  • Hong, Sa-Hoon;Ro, Kyung-Chul;Ryou, Hong-Sun;Lee, Seong-Hyuk
    • Journal of the Korean Society for Railway
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    • v.12 no.1
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    • pp.25-30
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    • 2009
  • The present study deals with numerical investigation for smoke behavior in rescue station by using the commercial CFD code (FLUENT Ver 6.3). With the use of the MVHS(Modify Volumetric Heat Source) model modified from the original VHS(Volumetric Heat Source) model, a 10 MW mode was adopted for simulation and the MVHS model can describe the generation of product and the oxygen consumption at the stoichiometric state. In addition, the present simulation includes the species conservation equations for the materialization of heat source and the estimation of smoke movement. From the results, the smoke flows are moving along the ceiling because of thermal buoyancy force and as time goes, the smoke gradually moves downward at the vicinity of the entrance. Moreover, without using ventilation, it is found that the smoke flows no longer spread across the cross-passages because the pressure in the non-accident tunnel is higher than that in the accident tunnel.

Optimization of energy efficiency through comparative analysis of factors affecting the operation with energy recovery devices on SWRO desalination process (역삼투막 해수담수화 공정에서 에너지 회수장치의 운영인자 비교분석을 통한 에너지 효율 최적화 연구)

  • Kim, Pooreum;Kim, Hyungsoo;Park, Junyoung;Kim, Taewoo;Kim, Minjin;Park, Kitae;Kim, Jihoon
    • Journal of Korean Society of Water and Wastewater
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    • v.32 no.1
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    • pp.1-10
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    • 2018
  • Recently, interest in the development of alternative water resources has been increasing rapidly due to environmental pollution and depletion of water resources. In particular, seawater desalination has been attracting the most attention as alternative water resources. As seawater desalination consumes a large amount of energy due to high operating pressure, many researches have been conducted to improve energy efficiency such as energy recovery device (ERD). Consequently, this study aims to compare the energy efficiency of RO process according to ERD of isobaric type which is applied in scientific control pilot plant process of each $100m^3/day$ scale based on actual RO product water. As a result, it was confirmed that efficiency, mixing rate, and permeate conductivity were different depending on the size of the apparatus even though the same principle of the ERD was applied. It is believed that this is caused by the difference in cross-sectional area of the contacted portion for pressure transfer inside the ERD. Therefore, further study is needed to confirm the optimum conditions what is applicable to the actual process considering the correlation with other factors as well as the factors obtained from the previous experiments.

Association between Shammah Use and Oral Leukoplakia-like Lesions among Adult Males in Dawan Valley, Yemen

  • Al-Tayar, Badr Abdullah;Tin-Oo, Mon Mon;Sinor, Modh Zulkarnian;Alakhali, Mohammed Sultan
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.18
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    • pp.8365-8370
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    • 2016
  • Background: Shammah is a traditional form of snuff dipping tobacco (a smokeless tobacco form) that is commonly used in Yemen. Oral mucosal changes due to the use of shammah can usually be observed in the mucosal surfaces that the product touches. The aim of this study was to determine the association between shammah use and oral leukoplakia-like lesions. Other associated factors were also determined. Materials and Methods: A cross sectional study was conducted on 346 randomly selected adult males. Multi-stage random sampling was used to select the study location. After completing the structured questionnaire interviews, all the participants underwent clinical exanimation for screening of oral leukoplakia-like lesions Clinical features of oral leukoplakia-like lesion were characterized based on the grades of $Ax{\acute{e}}ll$ et al (1976). Univariable logistic regression and multivariable logistic regression were used to assess the potential associated factors. Results: Out of 346 male participants aged 18 years and older, 68 (19.7%) reported being current shammah users. The multivariable analysis revealed that age, non-formal or primary level of education, former shammah user, current shammah user, and frequency of shammah use per day were statistically associated with the presence of oral leukoplakia-like lesions [Adjusted odds ratio (AOR) = 1.03; 95% confidence interval (CI) : 1.01, 1.06; P=0.006], (AOR=8.65; 95% CI: 2.81, 26.57; P=0.001), (AOR=3.65; 95% CI: 1.40, 9.50; P=0.008), (AOR=12.99; 95% CI: 6.34, 26.59; P=0.001), and (AOR=1.17; 95% CI: 1.02, 1.36; P=0.026), respectively. Conclusions: The results revealed oral leukoplakia-like lesions to be significantly associated with shammah use. Therefore, it is important to develop comprehensive shammah prevention programs in Yemen.

A Cross-Cultural Study on the Clothing Purchasing Behavior (의복 구매 행동의 비교 문화적 연구 - 한국과 미국 남자 대학생을 중심으로 -)

  • Han, Myung-Sook
    • The Research Journal of the Costume Culture
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    • v.17 no.1
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    • pp.162-176
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    • 2009
  • This study is to compare clothing purchasing behaviors between Korean and American college students, especially men. A theoretical study was reviewed, then the survey was performed. 200 questionnaires were utilized for this study. The SPSS 12.0 was used to analyze the technical statistics like average, frequency, t-test, $X^2$, and factor analysis. The result showed, first there were some differences in most of all the clothing Purchasing behaviors such as the reason for the purchases, the purchasing information source, the standard of selecting clothes and the standards of selecting stores. There were slight differences in the reasons for the purchases between Korean and American college men. Both of them said that they purchase clothes to satisfy their needs and the situation. The American college men were slightly higher than the Korean college men for both the need.situation and impulse motive. Regarding the information sources for the purchase, The Korean college men were slightly higher than the American college men for the media information sources and lower than them for the human information sources. Regarding the standard for store selection, the college men from both countries considered product service to be the most important. The American college men considered the convenient and store services to be more important than Korean college men. Regarding the standard fur clothes selection, the Korean college men considered the clothing that matched their personal styles to be the most important standard, however, the American college men considered the attractive from the opposite sex as the important standard.

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A Study on the Fashion Design of Hanji(Korean traditional paper) Textile Using the Formative Features of Scallop (가리비의 조형성을 이용한 한지직물 의상 디자인 연구)

  • Kwon, Min-Jung;Yu, Kum-Wha
    • Journal of the Korea Fashion and Costume Design Association
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    • v.13 no.3
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    • pp.149-163
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    • 2011
  • Hanjisa(Korean paper yarn), a new material made from the traditional Korean paper, has been developed through local R&D efforts, reflecting the current trend highly valuing environmental friendly. This new material is considered suitable for the 21C lifestyle and culture pursuing improved quality of human life and the environment. Therefore, this study aims to widely make known the originality and functions of the environmentally friendly Korean paper yarn, as well as to increase its commercial value. Furthermore, a new category of apparel design is presented by studying painting dyeing based on transformational tuck techniques and wax resist dyeing with formative features of repeated lines and rhythms of shells in order to implement three-dimensional and decorative artistic expressions. The texture of the Korean cotton paper yarn was particularly suitable to employ tuck and dyeing techniques Which express formative features of shell. Also, the material was useful for expressing the three-dimensional feelings with repeated curves and cross sections of shells. Moreover, paraffin resist dyeing and stitch techniques were used in order to avoid monotony and the images of shells visually materialized. Through the results stated above, this study could explore how to overcome obstacles to globalization of the Korean modern apparel such as its uniqueness, limit of materials or absense of internationality by applying modern design to the Korean paper fabrics. In the future, it is expected that more manufactures could produce and supply the new materials so as to make widely known the originality of the Korean paper fabrics and develop the material into a popular organic product fitting the modern lifestyle.

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Review of Mathematical Models in Performance Calculation of Screw Compressors

  • Stosic, Nikola;Smith, Ian K.;Kovacevic, Ahmed;Mujic, Elvedin
    • International Journal of Fluid Machinery and Systems
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    • v.4 no.2
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    • pp.271-288
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    • 2011
  • The mathematical modelling of screw compressor processes and its implementation in their design began about 30 years ago with the publication of several pioneering papers on this topic, mainly at Purdue Compressor Conferences. This led to the gradual introduction of computer aided design, which, in turn, resulted in huge improvements in these machines, especially in oil-flooded air compressors, where the market is very competitive. A review of progress in such methods is presented in this paper together with their application in successful compressor designs. As a result of their introduction, even small details are now considered significant in efforts to improve performance and reduce costs. Despite this, there are still possibilities to introduce new methods and procedures for improved rotor profiles, design optimisation for each specified duty and specialized compressor design, all of which can lead to a better product and new areas of application. A review of methods and procedures which lead to modern screw compressor practice is presented in this paper. This paper is intended to give a cross section through activities being done in mathematical modelling of screw compressor process through last five decades. It is expected to serve as a basis for further contributions in the area and as a challenge to the forthcoming generations of scientists and engineers to concentrate their efforts in finding future and more extended approaches and submit their contributions.