• Title/Summary/Keyword: cross hybridization

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Analysis of Non-segregated S-allele Strain by Single-Locus Hypothesis in Self-incompatible Brassica campestris (자가불화합성 Brassica campestris에 있어서 단일유전자좌가설에 의해 분리되지 않는 S-유전자 계통의 분석)

  • 노일섭
    • Journal of Plant Biology
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    • v.36 no.2
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    • pp.127-132
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    • 1993
  • Self-incompatibility in Brassica campestris is controlled by multi-allele system in a single genetic locus, the S locus, and it is elucidated that S-glycoproteins are S gene products. In this experiments, we examined the genetic mode(pollen tube behavior and segregation of S-glycoprotein), characteristic of S-glycoproteins and DNA constitution within nuclear genome on S gene family that unexplained by single locus model, and investigated the segregation pattern of S-glycoproteins in bred F1 generation. By diallel cross among the 15 plants within one family the existence of three types of homozygotes and three types of heterozygotes were observed, and segregation of S-allele could not explained by single locus model. From the results of IEF-immunoblot analysis for non-segregated individual plant, the segregation pattern of S specific bands was corresponded with results of diallel cross except with one case(SaSa genotype). The molecular weight of 6 different S-genotype varied in near by 50 kD, and each genotype expressed with 2 or 3 bands. Specific bands in SaSa, SbSb, ScSc has almost similar molecular weight between them. Southern analysis of genomic DNA probed with S-glycoprotein cDNA for 6 different genotypes revealed that there are clear difference in polymorphism, multiple bands of hybridization, when restriction enzymes of EcoR I were used. It could be assumed that there are several sequences related to the S-glycoprotein structural genes within their nuclear genome. Therefore, we suggested the possibilities that S-allele system could be controlled by multi-locus, that dominance-recessive interactions could be explained by modifier gene or supressor gene based on the results of abnormal segregation of S-glycoprotein in bred F1. The F2 analyses are progressing in now.

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Application and Its Typological Classification of Photographs Shown in Contemporary Art (현대미술에 나타난 사진의 활용과 그 유형학적 분류)

  • LEE, Kyung Ryul
    • Cross-Cultural Studies
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    • v.33
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    • pp.193-239
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    • 2013
  • A photograph, which was applied to contemporary art, plays a very important role in the whole of the multiplied and diversified contemporary arts today. The application of photos, which were shown in contemporary art following the 1950s, can be classified into five types according to role and function of a photo in the process of developing a work. However, this classification is shown a little ambiguously. That is because photos of being utilized by artists are indicated very diversely depending on their artistic strategy and situation. As the first type, a few painters utilize a photo as mirror of reality in order to materialize a challenging and revolutionary idea with going against traditional picture in their pictorial practice. As the second classification, especially American painters utilize a photo as material component of forming their picture like photo-montage or collage. The combined application of this medium is first doing genre de-construction and hybridization of post-modernism in the 1980s while ultimately aiming at social criticism or political satire. The third type implies particularly a case that avant-garde artists utilize a photo as evidence of a work. In this case, a photo is employed as evidence of a work, which disappears in the temporal space essentially like body art, land art, and performance. Also, as the fourth type is a case of utilizing a photo for artistic concept of a work, not pictorial practice of picture, it is utilized often as important artistic strategy of conceptual artists. The final type of utilizing photo is a case of applying photo as formative tool on behalf of dye-stuffs or crayon in order to record a section of reality, which always continues regardless of event or meaning any more, in the traditional picture. In this case, a work is indicated as a photo of having a form of picture, namely, as Forme-tableau. The main subject is indicated there as a daily case of being repeated always the extremely common and revived theme.

Development of a Phalaenopsis (P. Blume) Cultivar, 'Yellow Cream' with Striped Yellow Flower (황화 스트라이프 대륜계 호접란 신품종 '옐로우 크림' 육성)

  • Been, Chul-Gu;Kim, Jin-Ki;Kim, Soo-Kyeong;Noh, Chi-Wong
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.3
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    • pp.177-180
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    • 2011
  • A cultivar 'Yellow Cream' of Phalaenopsis(P. Blume) was developed by cross breeding and line selection at the Flower Research Institute, Gyeongnam ARES, from 2000 to 2008. Characteristic trials for this cultivar were carried out three times from 2006 to 2008. 'Yellow Cream' was developed from a crossing between P. 'Sogo Firework' and P. 'Sogo Gold'. 'Yellow Cream' exhibits light yellow flower color with pink stripe and favorable flower shape. Individual flowers are formed with parallel arrangement and have long life with more than 55 days. 'Yellow Cream'(Grant No.3232) was registered to the Korea seed and variety Service(KSVS) for commercialization in 2010 and suitable for the cultivation under greenhouse conditions in Korea.

Cymbidium 'Honey Girl' with White Color and Medium Plant Size (중형 백색계 다화성 심비디움 'Honey Girl' 육성)

  • Kim, Mi-Seon;Cho, Hae-Ryong;Lee, Hye-Kyung;Lim, Jin-Hee;Choi, Sung-Yul;Kim, Young-Jin
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.291-294
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    • 2008
  • Cymbidium 'Honey Girl' was developed in 2005 by the National Horticultural Research Institute, Rural Development Administration. This cultivar came from cross between Cym. 'Yakoto' and Cym. 'Marian Simplicity' in 1995. Fifty seven seedlings were obtained after planting and acclimatization in green house. In 1999, a line was selected by its performance such as flower color, leaf shape, flower stalk, and vigorous growth which was named 'Wongyo F1-13'. The line code '9521928'($V_2$) had uniformity and excellent characteristics. The selected line was named as 'Honey Girl' after second characteristics test. This cultivar had white color ('WN 155B') and medium sized leaves and flowers (average leaf length is about 57 cm and width of flower is about 7.7cm) with many flowers and erect flower stalks. Blooming begins from the end of November to December under the normal culture conditions.

A New Cultivar Cymbidium 'White Princess' with White Color and Vigorous Growth (생육이 강한 대형 백색계 심비디움 'White Princess' 육성)

  • Kim, Mi-Seon;Cho, Hae-Ryong;Lee, Hye-Kyung;Lim, Jin-Hee;Choi, Sung-Yul;Kim, Young-Jin
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.295-298
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    • 2008
  • A new Cymbidium cultivar 'White Princess' was released by the National Horticultural Research Institute (NHRI) in 2005. A cross was made in 1995 between 'Cymbidium 2113', light purple colored petal and medium plant sized variety, and 'Lucky Rainbow Randevous', a dark purple petal with red lip and large type variety. The ninety progenies were obtained after planting and acclimatization in green house. In 2000, a line (9526747) was selected and multipliticed after test of its flower color, leaf shape, flower stalk, and vigorous growing habit. After evaluation trial for two growing seasons, the selected line was named as 'White Princess'. The 'White Princess' has white petals (WN 155A) with red lip (RP59D) color and large sized leaves, and flowers (leaf length is about 88 cm and flower diameter is about 8 cm) with semi-erect flower stalks. Blooming started from December to January in optimal culture conditions. High temperature (more than $30^{\circ}C$) and low light intensity(less than 10,000Lux) should be avoided for the appropriate growth.

Gold Shell Nanocluster Networks in Designing Four-Branch (1×4) Y-Shape Optical Power Splitters

  • Ahmadivand, Arash;Golmohammadi, Saeed
    • Journal of the Optical Society of Korea
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    • v.18 no.3
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    • pp.274-282
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    • 2014
  • In this study, closely spaced Au nanoparticles which are arranged in nanocluster (heptamer) configurations have been employed to design efficient plasmonic subwavelength devices to function at the telecommunication spectrum (${\lambda}$~1550 nm). Utilizing two kinds of nanoparticles, the optical properties of heptamer clusters composed of Au rod and shell particles that are oriented in triphenylene molecular fashion have been investigated numerically, and the cross-sectional profiles of the scattering and absorption of the optical power have been calculated based on a finite-difference time-domain (FDTD) method. Plasmon hybridization theory has been utilized as a theoretical approach to characterize the features and properties of the adjacent and mutual heptamer clusters. Using these given nanostructures, we designed a complex four-branch ($1{\times}4$) Y-shape splitter that is able to work at the near infrared region (NIR). This splitter divides and transmits the magnetic plasmon mode along the mutual heptamers arrays. Besides, as an important and crucial parameter, we studied the impact of arm spacing (offset distance) on the guiding and dividing of the magnetic plasmon resonance propagation and by calculating the ratio of transported power in both nanorod and nanoshell-based structures. Finally, we have presented the optimal structure, that is the four-branch Y-splitter based on shell heptamers which yields the power ratio of 23.9% at each branch, 4.4 ${\mu}m$ decaying length, and 1450 nm offset distance. These results pave the way toward the use of nanoparticles clusters in molecular fashions in designing various efficient devices that are able to be efficient at NIR.

Studies on the Intergeneric Crosses in Triticeae I. Influence of Crossing Methods and Chemicals on the Hybridization Rate in Wheat-barley Crossings (맥류의 종속간 교잡에 관한 연구 제1보 교배방법 및 교배촉진물질의 처리가 교잡종자 생산에 미치는 영향)

  • Kim, B.Y.;Hong, B.H.;Cho, C.H.;Je, S.Y.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.27 no.2
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    • pp.111-115
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    • 1982
  • The trials were carried out for improvement of early maturing wheat varieties, desease resistant and good quality through transfering good characters of barley to the wheat, during 1980-'81. For these purpose, Chinese Spring, a wheat variety was used as male and Manker 16 a barley variety, as female parents. EACA($\varepsilon$-Amino Caprolic Acid) known as an inhibitor of blood coagulation was injected to all barley from 14 days until heading with 1$m\ell$ per culm at the level of 500ppM. $GA_3$ solution which accelerates pollen activity was also used with 75ppM concentration after pollination for two days including check to determine the single effect of EACA. Two different methods of the cross were used. One was bud-pollination, another was post-pollination. The result indicated that the post-pollination was better than bud-pollination in terms of pollination rate, even though formation rate of normal embryo was seemingly adversed. The new plant derived from barley-wheat cross was similar in phenotype to the wheat.

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Cloning and Characterization of Liver cDNAs That Are Differentially Expressed between Chicken Hybrids and Their Parents

  • Sun, Dong-Xiao;Wang, Dong;Yu, Ying;Zhang, Yuan
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.12
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    • pp.1684-1690
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    • 2005
  • Using mRNA differential display technique, we investigated differential gene expression in hybrids relative to their parents in a diallel cross involving four chicken breeds in order to provide an insight into the molecular basis of heterosis in chicken. The results indicated that there was extensive differential gene expression between chicken F1 hybrids and their parents which was classified into four kinds of patterns as following: (1) bands only detected in hybrid F1; (2) bands only absent in hybrid F1; (3) bands only detected in parent P1 or P2; (4) bands absent in parent P1 or P2. Forty-two differentially expressed cDNAs were cloned and sequenced, and their expression patterns were confirmed by Reverse-Northern dot blot. Sequence analysis and database searches revealed that genes showed differential expression between hybrid and parents were regulatory and functional genes involved in metabolism, mRNA splicing, transcriptional regulation, cell cycles and protein modification. These results indicated that hybridization between two parents can cause changes in expression of a variety of genes. In conclusion, that the altered pattern of gene expression in hybrids may be responsible for heterosis in chickens.

A New Hybrid, Dark Pink Spotted Type Phalaenopsis 'Pink Marble'

  • Kim, Mi-Seon;Lee, Young-Ran;Rhee, Hye-Kyung;Park, Sang-Kun;Shin, Hak-Ki;Jung, Hyang-Young;Lim, Jin-Hee
    • Horticultural Science & Technology
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    • v.29 no.5
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    • pp.503-506
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    • 2011
  • A new hybrid, Phalaenopsis 'Pink Marble' was made by the National Institute of Horticultural and Herbal Science, Rural Development Administration, in 2005. This hybrid was selected from self-crossed progenies of P. '21-1' (collected number) in 1999. In 2001, one line was selected based on the aspects such as flower color, leaf shape, flower stalk, and vigorous growth. Trials were conducted from 2003 to 2005 for evaluation and selection of this cultivar. 'Pink Marble' had a medium flowering habit and a dark pink spot (RHS, RPN74B) on white petal and sepal when fully opened. The number of flowers on each peduncle was 7.5, and flower diameter was 52.3 cm. The general impression of petals and sepals is a plate shape. The thick sepal could extend the long flowering time. The average length of leaf and peduncle were 16.5 cm and 6.8 cm, respectively. It had a half-erect leaf form, and was a fast-growing cultivar. This hybrid is relatively easy to clone.

Interspecific Hybridization between Triploid Hybrid Fish, Cobitis sinensis - longicorpus and Two Diploid Species from Korea (잡종 3배체 어류 기름종개 - 왕종개 잡종군의 종간 교잡실험)

  • Kim, Ik-Soo;Lee, Eun-Hee
    • Korean Journal of Ichthyology
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    • v.7 no.1
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    • pp.71-78
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    • 1995
  • Cobitis sinensis - longicorpus complex were commonly found in the upper stream of the Nakdong River in Korea and consisted of mostly with diploid and triploid karyotype forms. Among them, the triploid females of C. sinensis - longicorpus were artificially crossed with diploid males from C. sinensis and diploid males from C. longicorpus, respectively. The progenies from each cross produced the diploid individuals that possessed the paternal characters in their karyotypes and their body color patterns. Based on this results, we can assume that the triploid females, C. sinensis - longicorpus have a peculiar reproductive mode eliminating an uneven genome by the primary meiotic division and then producing a haploid ovum by secondary meiosis.

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