• Title/Summary/Keyword: crop disease

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The Gene Pyramiding Effect of qBK1 and qFfR1 Genes of Major Resistance Genes in Bakanae Disease

  • Sais-Beul Lee;Sumin Jo;Jun-Hyun Cho;Nkulu Rolly Kabange;Ji-Yoon Lee;Yeongho Kwon;Ju-Won Kang;Dongjin Shin;Jong-Hee Lee;You-Cheon Song;Jong-Min Ko;Kyung-Min Kim;Dong-Soo Park
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.65-65
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    • 2020
  • Bakanae disease, caused by Gibberella fujikuroi, is one of the most devastating diseases threatening rice production in Korea. In recent years, the incidence of bakanae disease became alarming due to the mechanical transplanting practice where the spread of bakanae can be amplified during accelerating seeds growth, due to the use of seeding boxes. The development of resistant rice cultivars could be the primary and effective method for controlling bakanae disease. However, the effects of individual resistance genes are relatively small. Therefore, pyramiding of bakane R genes in rice breeding is a promising strategy having a high potential to mitigate the advert effects of bakanae disease. This study employed a gene pyramiding approach to develop bakanae disease resistant rice lines carrying qBK1, qFfR1 introduced from rice line MY299BK and cv. Nampyeong, respectively. The MY299BK carries qBK1 introduced from cv. Shingwang, which was found to have a high resistance compare to Nampyeong. In addition, the pyramiding effect of the qBK1 and qFfR1 resistance genes were investigated, and the presence or absence of these genes helped us investigate their interaction through bioassay method and MAS. Furthermore, the distribution of resistance in the population showed a biased distribution toward resistance in the F6:7 populutions. However, we could not confirm the accumulation effect of the resistance gene, but the difference between the two genes by the SN2 marker was confirmed. Therefore, the qBK1 gene harbored by MY299BK appears to be different from the qFfR1 carried by Nampyeong, suspected to possess a different bakanae disease resistant gene different from those found in MY299BK and Nampyeong.

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An Efficient Disease Inspection Model for Untrained Crops Using VGG16 (VGG16을 활용한 미학습 농작물의 효율적인 질병 진단 모델)

  • Jeong, Seok Bong;Yoon, Hyoup-Sang
    • Journal of the Korea Society for Simulation
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    • v.29 no.4
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    • pp.1-7
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    • 2020
  • Early detection and classification of crop diseases play significant role to help farmers to reduce disease spread and to increase agricultural productivity. Recently, many researchers have used deep learning techniques like convolutional neural network (CNN) classifier for crop disease inspection with dataset of crop leaf images (e.g., PlantVillage dataset). These researches present over 90% of classification accuracy for crop diseases, but they have ability to detect only the pre-trained diseases. This paper proposes an efficient disease inspection CNN model for new crops not used in the pre-trained model. First, we present a benchmark crop disease classifier (CDC) for the crops in PlantVillage dataset using VGG16. Then we build a modified crop disease classifier (mCDC) to inspect diseases for untrained crops. The performance evaluation results show that the proposed model outperforms the benchmark classifier.

Performance Comparison of Base CNN Models in Transfer Learning for Crop Diseases Classification (농작물 질병분류를 위한 전이학습에 사용되는 기초 합성곱신경망 모델간 성능 비교)

  • Yoon, Hyoup-Sang;Jeong, Seok-Bong
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.44 no.3
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    • pp.33-38
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    • 2021
  • Recently, transfer learning techniques with a base convolutional neural network (CNN) model have widely gained acceptance in early detection and classification of crop diseases to increase agricultural productivity with reducing disease spread. The transfer learning techniques based classifiers generally achieve over 90% of classification accuracy for crop diseases using dataset of crop leaf images (e.g., PlantVillage dataset), but they have ability to classify only the pre-trained diseases. This paper provides with an evaluation scheme on selecting an effective base CNN model for crop disease transfer learning with regard to the accuracy of trained target crops as well as of untrained target crops. First, we present transfer learning models called CDC (crop disease classification) architecture including widely used base (pre-trained) CNN models. We evaluate each performance of seven base CNN models for four untrained crops. The results of performance evaluation show that the DenseNet201 is one of the best base CNN models.

Use of Sodium Hypochlorite for the Control of Bakanae Disease in Rice (벼 키다리병 방제를 위한 차아염소산나트륨 이용)

  • Shin, Dong Bum;Goh, Jaeduk;Lee, Bong Choon;Kang, In Jeong;Kang, Hang-Won
    • Research in Plant Disease
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    • v.20 no.4
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    • pp.259-263
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    • 2014
  • For application of sodium hypochlorite as a seed disinfectant to the control of bakanae disease caused by Gibberella fujikuroi in rice, we investigated the effects of sodium hypochlorite for antifungal activity, eliminating fungus from seeds and reducing disease occurrence in vitro and greenhouse. The viability of the pathogen was significantly reduced at $80{\mu}l/l$ concentration of sodium hypochlorite, and the pathogens did not grow at over $100{\mu}l/l$ concentration of sodium hypochlorite. The effect of eliminating fungus was 90% at treatment of 0.3% sodium hypochlorite solution to infected rice seeds for eight hours. When the rice seeds were soaked into 0.5% and 0.3% sodium hypochlorite solutions for twelve hours, the disease incidences of rice seedling were remarkably reduced to 4.3% and 4.7%, respectively, compared to 97.3% of non-treatment control. The rates of seedling stand were 29.1% and 26.9% higher with the sodium hypochlorite treatment than that of non-treatment control. When prochloraz and sodium hypochlorite was treated to naturally severely infested rice seeds with bakanae disease, the disinfection effect was higher than that of prochloraz alone treatment. When the seeds were soaked in sodium hypochlorite before or after prochloraz, the rate of seed contamination was low as 4.0% or 6.3%, respectively, compared to prochloraz alone as 13.7%. The disease incidence was low as 3.7% or 8.3%, respectively, compared to prochloraz alone as 14.3%. The disinfection effect of treatment with prochloraz after sodium hypochlorite was higher than that of treatment with prochloraz before sodium hypochlorite.

Effects of Different Seeding Rates on Disease Incidences of Wheat Sharp Eyespot and Selection of Fungicides (밀 잎집눈무늬병의 발생에 파종량이 미치는 영향과 방제 약제 선발)

  • Park, Jong-Chul;Lee, Eun-Sook;Cho, Kwang-Min;Lee, Mi-Ja;Kang, Chun-Sik;Choi, Jae-Seong
    • Research in Plant Disease
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    • v.18 no.1
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    • pp.10-16
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    • 2012
  • This study was conducted to examine the effects of the seeding rate on the disease incidence of sharp eyespot(Rhizoctonia cerealis) on three different varieties and to select effective chemicals to control the disease. When the seeds were sown twice as many as the recommendation, the disease incidence increased by approximately 13%. However, the susceptible variety 'Jopummil' alone showed the significantly enhanced disease incidence at a two-fold seeding rate. Two chemicals such as Hexaconazole EC and Tebuconazole EC highly inhibited the fungal growth on agar medium. However, two strobilurin fungicides such as Pyraclostrobin EC and Trifloxystrobin SC were relatively weak. The fungicides tested displayed the similar in vivo antifungal activities as in vitro activities. Hexaconazole EC and Tebuconazole EC showed the strongest both protective and curative activities and the protective activities of the chemicals were generally higher than the curative activities. Hexaconazole EC and Tebuconazole EC controlled the disease by 64% and 73%, respectively, and the two chemicals reduced the disease by 45% and 39%, respectively, when they were applied one day after pathogen inoculation. These results indicate that both Hexaconazole EC and Tebuconazole EC could be used to control sharp eyespot on wheat.

Breeding Hybrid Rice with Genes Resistant to Diseases and Insects Using Marker-Assisted Selection and Evaluation of Biological Assay

  • Kim, Me-Sun;Ouk, Sothea;Jung, Kuk-Hyun;Song, Yoohan;Le, Van Trang;Yang, Ju-Young;Cho, Yong-Gu
    • Plant Breeding and Biotechnology
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    • v.7 no.3
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    • pp.272-286
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    • 2019
  • Developing elite hybrid rice varieties is one important objective of rice breeding programs. Several genes related to male sterilities, restores, and pollinators have been identified through map-based gene cloning within natural variations of rice. These identified genes are good targets for introducing genetic traits in molecular breeding. This study was conducted to breed elite hybrid lines with major genes related to hybrid traits and disease/insect resistance in 240 genetic resources and F1 hybrid combinations of rice. Molecular markers were reset for three major hybrid genes (S5, Rf3, Rf4) and thirteen disease/insect resistant genes (rice bacterial blight resistance genes Xa3, Xa4, xa5, Xa7, xa13, Xa21; blast resistance genes Pita, Pib, Pi5, Pii; brown planthopper resistant genes Bph18(t) and tungro virus resistance gene tsv1). Genotypes were then analyzed using molecular marker-assisted selection (MAS). Biological assay was then performed at the Red River Delta region in Vietnam using eleven F1 hybrid combinations and two control vatieties. Results showed that nine F1 hybrid combinations were highly resistant to rice bacterial blight and blast. Finally, eight F1 hybrid rice varieties with resistance to disease/insect were selected from eleven F1 hybrid combinations. Their characteristics such as agricultural traits and yields were then investigated. These F1 hybrid rice varieties developed with major genes related to hybrid traits and disease/insect resistant genes could be useful for hybrid breeding programs to achieve high yield with biotic and abiotic resistance.

Detection of Rice Disease Using Bayes' Classifier and Minimum Distance Classifier

  • Sharma, Vikas;Mir, Aftab Ahmad;Sarwr, Abid
    • Journal of Multimedia Information System
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    • v.7 no.1
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    • pp.17-24
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    • 2020
  • Rice (Oryza Sativa) is an important source of food for the people of our country, even though of world also .It is also considered as the staple food of our country and we know agriculture is the main source country's economy, hence the crop of Rice plays a vital role over it. For increasing the growth and production of rice crop, ground-breaking technique for the detection of any type of disease occurring in rice can be detected and categorization of rice crop diseases has been proposed in this paper. In this research paper, we perform comparison between two classifiers namely MDC and Bayes' classifiers Survey over different digital image processing techniques has been done for the detection of disease in rice crops. The proposed technique involves the samples of 200 digital images of diseased rice leaf images of five different types of rice crop diseases. The overall accuracy that we achieved by using Bayes' Classifiers and MDC are 69.358 percent and 81.06 percent respectively.

Change in Occurrence of Rice stripe virus Disease (벼줄무늬잎마름병의 발생변화)

  • Lee, Bong Choon;Cho, Sang-Yun;Yoon, Young-Nam;Kang, In Jeong;Lee, Jong Hee;Kwak, Do Yeon;Shin, Dong Bum;Kang, Hang-Won
    • Research in Plant Disease
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    • v.18 no.4
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    • pp.402-405
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    • 2012
  • We surveyed the occurrence of Rice stripe virus (RSV) disease in 672 fields from 29 rice representative area in July 2012 as nationwide survey for RSV occurrence since 2008. We confirmed occurrence of virus disease in 18 areas, in west coast region including Secheon, Taean, Buwan and Cheorwon. RSV incidence rates of plant in Sacheon and Buan were less than 0.01% and 0.15%, respectively, showing similar rate with the nationwide survey carried out in 2008, whereas incidence rate of field declined from 19.9% in 2008 to 4.9% in 2012. Earlier, RSV occurred largely across the southern region of Korea. In 2001, RSV disease was found in Gangwha and Gyeonggi-do, the northern region of Korea. In 2007, RSV appeared in west coast; Buan in Jeollabuk-do and Seocheon in Choongnam-do. After migration of the vector, small brown plant hopper, from China in 2009, RSV is becoming a pandemic.