• Korean Journal of Environmental Biology
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• v.32 no.1
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• pp.75-87
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• 2014

This study describes about the spatio-temporal distributions in phytoplankton community such as species composition, standing crop and dominant species from May 2006 to February 2007 in the Jangsu bay and the northwestern parts of Gamak bay. Based on the principal component analysis (PCA) of the environmental factors as well as biological parameters, the bio-oceanographical characteristics were analysed. A total of 83 species of phytoplankton belonging to 47 genera were identified. Whereas diatoms and dinoflagellates occupied more than 65% and 30% of total species, respectively. The annual dominant species were Chaetoceros affinis, Paralia sulcata and Bacillaria paxillifera in spring, Chaetoceros didymus, Ch. affinis and Octactis octonaria in summer, Skeletonema costatum-like species and B. paxillifera in autumn. Moreover phytoplankton cell density was ranged between 3.1 $cells{\cdot}mL^{-1}$ in spring and 521.0 $cells{\cdot}mL^{-1}$ in winter. It fluctuated with an annual mean of 76.0 $cells{\cdot}mL^{-1}$ between the lowest value of 7.6 $cells{\cdot}mL^{-1}$ in spring and the highest value of 220.2 $cells{\cdot}mL^{-1}$ by Skeletonema costatum-like species in winter. Briefly, the phytoplankton cell density in the mixing seasons was higher in comparison with the other seasons. According to the PCA, the biological oceanographic characteristics of the Jangsu bay was affected by the introduction of outside seawater particularly in temperature increasing seasons, and the other seasons, it may be described the light intensity, and mix between inner and outer bay sea waters.

• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.235-242
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• 2004

A system for the production of transgenic plants has been developed for Italian ryegrass(Lolium mult리orum Lam.) via Agrobacterium-mediated transformation of embryogenic callus. Mature seed-derived calli were infected and co-cultured with Agrobacterium EHA101 carrying standard binary vector pIG121Hm encoding the hygromycin phosphotransferase(HPT), neomycin phosphotransferase II (NPTII) and intron-oontaining $\beta$g1ucuronidase( intron-GUS) genes in the T-DNA region. The effects of several factors on transformation and the expression of the GUS gene were investigated. Inclusion of 200${\mu}M$ acetosyringone(AS) in inoculation and co-cultivation media lead to a significant increase in stable transformation efficiency. Increasing Agrobacterium cell density up to 1.0 in $OD_{600}$ during infection increased transfonnation efficiency of embryogenic calli. The highest transfonnation efficiency was obtained when embryogenic calli were incoulated with Agrobacterium in the presence of 0.1% Tween20 and 200${\mu}M$ AS. Hygromycin resistant calli were developed into complete plants via somatic embryogenesis. GUS histochemical assay and PCR analysis of transgenic plants demonstrated that transgenes were integrated into the genome of Italian ryegrass.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.95-98
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• 2010

One interesting parasitic forming fungus, being grown on the eggs of Phallus impudicus was collected from Mulchajoreum and on woods of Saryeoni in Jeju island. This taxon identified as a Podostroma solmsii (E. Fisch.) S. Imai f. octospora Yoshim. Doi (Hyporeaceae) based on morphological characteristics. According to the interpretation of Doi(1978), this taxon hsving the stipitate, genus Podostroma, family Hypocreaceae, Hypocreales, Ascomycota was identified as Podostroma solmsi f. octospora and its korean common name designated by authors. This species is characterized in having the fusiforme to ellipsoidal, unicellular and warted ascospores without septate and with blunt apiculus at each end and mycoparasitic habitat. Although there is some mycologists suggest that this taxon having above characters needs to be derived from Hypomyces. But authors are primarily going to record this species as a named "Podostroma solmsii (E. Fisch.) S. Imai f. octospora Yoshim. Doi" for the fungus flora of Korea. In a future, it is need in detail to compare their DNA sequencing data on the type species and some related species. All collections cited here are deposited in the Herbarium Conservation Center of National Academy of Agricultural Sciences.

• Hwankyungkyoyuk
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• v.20 no.2
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• pp.93-107
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• 2007

Livestock agriculture plays an essential role Hongsung's economy. Unfortunately, if manure is manage properly it can contaminate water and pollute the air. The purposes of this study were to provide awareness of environmental impact and to design educational programs with sustainable agriculture. I used the specific steps of processes. First, collecting informations through survey on environmental awareness of livestock farmers in Hongsung-gun and recognizing the tendencies. Second, analyzing on educational status of livestock farmers. Third, finding objectives of environmental educational programs. At the end of processes, establishing programs which concerning on environmental education. I used two research methods to gather and analyze the data for developing an environmental education program. The first one is a general survey among 50 livestock farmers from Hongseong-gun, Chungcheongnam-do. The second one is the in-depth interviews with specialists in environmental education and government officials from the central government - the Ministry of Agriculture and Forestry - and the local government - Hongsung-gun who are in charge of livestock farming. I found the following results from this research. First, most livestock farmers replied with 'highly likely' to the five items on the recognition of the necessity of environment preservation, whereas only 38.7% answered with 'likely' to the item on the environmental pollution caused by livestock farming. This shows the tendency that they have less recognition of the reality than necessary. Second, most of the contents among 5 areas(knowledge, recognition, function, attitude, and participation) are the knowledge areas. That results are analyzed training materials for livestock farmers made by Hongsung-gun in 2004 and in 2005 by using goal categories suggested in the Tbilisi Resolution. Third, after analyzing the survey and in-depth interviews, I found that the need for education to recognize the environmental pollution, environmental protection and recycling of the livestock waste. We can summarize conclusions from this research. First, we need on educational program improve the livestock farmers' recognition of importance in environmental protection. Second, it is necessary to develop educational program with the viewpoint that livestock waste is not one of the culprits of the environmental pollution, but a recyclable resource. Third, contents of the environmental education program, should include all the educational elements, namely, knowledge, recognition, attitude, function and participation. The education for livestock farmers requires a research on the environmental education program that can be used with crop farmers who can use the resources recycled from the livestock waste from the viewpoint of recycling resources. This research is focused on the collection and analysis of the basic data for developing an environmental education program and the composition of the program. A further research on the implementation and assessment of this program is required.

• Journal of Sericultural and Entomological Science
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• v.52 no.2
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• pp.129-133
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• 2014

We analyzed rutin content using mulberry genetic resources. They were grown under the same environment and conditions. Mulberry leaves were collected and then freeze-dried and powdered for rutin test. As a result, mean content of mulberry strains was $0.38{\pm}0.17%$, and the coefficient of variation (CV) was 45.7%. The variation was greatly severe. Among the tested strains, 'Jangloe' was showed the highest content of 0.97%, whereas 'Sungsu 3' was showed the lowest content of 0.01% respectively. Finally we selected rutin high-containing 6 strains. They are as follows. 'Jangloe', 'Pumbo 6', 'Youjin', 'Kaeryangjeonjeon', 'Baekhak', and 'Pumbo 32' which are more than twice of the overall average content.

• Weed & Turfgrass Science
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• v.1 no.4
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• pp.24-30
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• 2012

This study was conducted to select the cover crops in organic apple orchard by estimating the coverage of each cover crops and time-periodic weed occurrence. Seeding of Festuca myuros and Trifolium repens showed high contents of soil organic matter and phosphorus as compared with control. Seeding of Trifolium sp., Hordeum vulgare and Secale cereal reduced relatively occurrence of weed in May and S. cereal among others showed the greatest effect in June. In July, seeding F. arundinaceae and Poa pratensis showed low coverage and a lot of weeds occurred in their field. Fifty one species were occurred as weeds and it needs to removal of high and broadleaf weeds such as Chenopodium ficifolium, C. album var. centrorubru and Humulus japonicus in spring to increase the rate of ground cover. P. pratensis having the lowest cover rate showed the similar dry weight of weeds as compared with control. Trifolium sp. and Persicaria hydropiper had a high correlation with contents of soil organic matter and C. album var. centrorubru had a correlation with contents of soil phosphorus by CCA analysis.

• Research in Plant Disease
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• v.12 no.2
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• pp.108-114
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• 2006

This study was conducted to elucidate the interactions among soil microorganisms in a special field where one, two or three of N, P, K fertilizers were continuously not applied for 29 years. Crop yield (barley, soybean), soil chemical properties and microflora and microfauna including nematodes, nematophagous fungi, actinomycetes, bacteria, and fungi were examined for two years. Tylenchorhynchus sp. was the most important plant-parasitic nematode (range $11{\sim}642/300 cm^3$ soil) followed by Pratylenchus sp. and Helicotylenchus sp. Among nematophagous fungi, Monacrosporium spp. was the most frequently found followed by Harposporium sp. and Cystopage sp. In general, plots treated with phosphate fertilizer yielded more, had more nematodes, bacteria and actinomycetes. In contrast, total fungal population densities including nematophagous fungi, Cystopage sp. and Harposporium sp. were in reverse; they were more abundant in the plots with lower phosphate contents. Phosphate and pH are positively correlated and two most important determining factors for the population density of soil organisms under investigation. According to correlation analysis, Ca, Mg, and $SiO_2$ contents in soil and population densities of Tylenchorhynchus sp., saprophitic nematodes, actinomycetes, and bacteria were positively correlated with pH, but were negatively correlated with fungal population densities. We hope that the study will add an additional knowledges to understand our mysterious underworld.

• Molecules and Cells
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• v.27 no.4
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• pp.467-473
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• 2009

Our previous study suggested that OsBWMK1, a gene which encodes a member of the rice MAP kinase family, generates transcript variants which show distinct expression patterns in response to environmental stresses. The transcript variants are generated by alternative splicing and by use of alternative promoters. To test whether the two alternative promoters, pOsBWMK1L (promoter for the OsBWMK1L splice variant) and pOsBWMK1S (promoter for the OsBWMK1S splice variant), are biologically functional, we analyzed transgenic plants expressing GUS fusion constructs for each promoter. Both pOsBWMK1L and pOsBWMK1S are biologically active, although the activity of pOsBWMK1S is lower than that of pOsBWMK1L. Histochemical analysis revealed that pOsBWMK1L is constitutively active in most tissues at various developmental stages in rice and Arabidopsis, whereas pOsBWMK1S activity is spatially and temporally restricted. Furthermore, the expression of pOsBWMK1S::GUS was upregulated in response to hydrogen peroxide, a plant defense signaling molecule, in both plant species. These results suggest that the differential expression of OsBWMK1 splice variants is the result of alternative promoter usage and, moreover, that the mechanisms controlling OsBWMK1 gene expression are conserved in both monocot and dicot plants.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.89-94
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• 2013

This study was carried out to calculate the genic values of days for flowering in commercial breeding lines of Korean hot pepper (Capsicum annuum L.). Two breeding lines of pepper '#2132' ($P_1$) early-flowering, and '#1308' ($P_2$) medium-late flowering, and their $F_1$ and $F_2$ generations were used in this study. By using partitioning method (Thseng and Hosokawa, 1971, 1972), it was possible to estimate, from the $F_2$ generations, the number of effective factor pairs differentiating the two parents. It was found out that the two parents were differentiated by two effective factor pairs, A:a and B:b. In the breeding lines used, the inheritance of days to flowering showed that the $F_1$ flowered a little earlier than the earlier flowering parent through the effect of over-dominance. However, $F_2$ flowered earlier or later than both parents through transgressive segregation. Conclusively, the magnitude of genic effects of A-a gene in flowering days was -13.81 days, and B-b gene was -6.73 days. The interaction between the two non-allelic factors using partitioning method was -5.26 days.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.369-376
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• 2009

Cowpea might have been introduced from China to Korea and cultivated for several hundred years but it has never been a staple food crop in Korea. In this study, genetic diversity of 492 Korean cowpea landrace accessions that have passport information was estimated using six SSR markers. The mean of Weir's gene diversity was 0.665 from all accessions investigated in the study. Cowpea gene diversity of six local provinces in Korea was ranged from 0.370 in accessions of Gangwon to 0.680 in Jeonra provinces. Low gene diversity of the cowpea genepool of Gangwon province was probably derived from relatively few introductions. Especially SSR markers VM36 and VM39 seem to be good markers to distinguish the Gangwon accessions from others by occurring at a specific locus with higher than 78% of allele frequency. Except for the Gangwon province with the low genetic diversity, gene diversity of cowpea accessions from other provinces was ranged from 0.600 to 0.680 indicating no big differences among provinces. Distribution pattern of the allele frequencies was similar among the other provinces. This may reveal that Korean farmers might exchange cowpea seeds easily with even their neighbors with geographical barriers. A core collection, 100 landraces, ca. 20% of base collection, was developed at the 70% of a similarity coefficient level using random sampling approaches after stratification of the entire landrace collection based on the phenetic dendrogram. The variability of SSR in the base and core collections of Korean cowpea landrace was compared by calculating Weir's gene diversity. The mean of Weir's gene diversity of the core was 0.707 while that of the base collection was 0.665. The higher diversity index in the core collection indicates that it maintains the initial variability and well represents the base collection. The core collection included one of determinate accession (IT 216155) and two of no branching type accessions (IT 103959 and IT 161024). The core collection could be used to guide more efficient management and utilization of the entire collection. This core collection should be revised periodically as additional accessions are collected and further characterization is conducted.