• Title/Summary/Keyword: critical experiment

Search Result 944, Processing Time 0.027 seconds

Analysis on the Shear Behavior of Existing Reinforced Concrete Beam-Column Structures Infilled with U-Type Precast Wall Panel (U형 프리캐스트 콘크리트 벽패널로 채운 기존 철근 콘크리트 보-기둥 구조물의 전단 거동 분석)

  • Ha, Soo-Kyoung;Son, Guk-Won;Yu, Sung-Yong;Ju, Ho-Seong
    • Journal of the Korea institute for structural maintenance and inspection
    • /
    • v.19 no.6
    • /
    • pp.18-28
    • /
    • 2015
  • The purpose of this study is to develop a new seismic resistant method by using precast concrete wall panels for existing low-rise, reinforced concrete beam-column buildings such as school buildings. Three quasi-static hysteresis loading tests were performed on one unreinforced beam-column specimen and two reinforced specimens with U-type precast wall panels. The results were analyzed to find that the specimen with anchored connection experienced shear failure, while the other specimen with steel plate connection principally manifested flexural failure. The ultimate strength of the specimens was determined to be the weaker of the shear strength of top connection and flexural strength at the critical section of precast panel. In this setup of U-type panel specimens, if a push loading is applied to the reinforced concrete column on one side and push the precast concrete panel, a pull loading from upper shear connection is to be applied to the other side of the top shear connection of precast panel. Since the composite flexural behavior of the two members govern the total behavior during the push loading process, the ultimate horizontal resistance of this specimen was not directly influenced by shear strength at the top connection of precast panel. However, the RC column and PC wall panel member mainly exhibited non-composite behavior during the pull loading process. The ultimate horizontal resistance was directly influenced by the shear strength of top connection because the pull loading from the beam applied directly to the upper shear connection. The analytical result for the internal shear resistance at the connection pursuant to the anchor shear design of ACI 318M-11 Appendix-D, agreed with the experimental result based on the elastic analysis of Midas-Zen by using the largest loading from experiment.

Understanding Problem-Solving Type Inquiry Learning and it's Effect on the Improvement of Ability to Design Experiments: A Case Study on Science-Gifted Students (문제해결형 탐구학습에 대한 인식과 학습이 실험 설계 능력에 미친 효과 : 과학 영재학생들에 대한 사례 연구)

  • Ju, Mi-Na;Kim, Hyun-Joo
    • Journal of The Korean Association For Science Education
    • /
    • v.33 no.2
    • /
    • pp.425-443
    • /
    • 2013
  • We developed problem-solving type inquiry learning programs reflecting scientists' research process and analyzed the activities of science-gifted high school students, and the understanding and the effects of the programs after implementation in class. For this study, twelve science-gifted students in the 10th grade participated in the program, which consisted of three different modules - making a cycloidal pendulum, surface growth, and synchronization using metronomes. Diet Cola Test (DCT) was used to find out the effect on the improvement of the ability to design experiments by comparing pre/post scores, with a survey and an interview being conducted after the class. Each module consisted of a series of processes such as questioning the phenomenon scientifically, designing experiments to find solutions, and doing activities to solve the problems. These enable students to experience problem-solving type research process through the program class. According to this analysis, most students were likely to understand the characteristics of problem-solving type inquiry learning programs reflecting the scientists' research process. According to the students, there are some differences between this program class and existing school class. The differences are: 'explaining phenomenon scientifically,' 'designing experiments for themselves,' and 'repeating the experiments several times.' During the class students have to think continuously, design several experiments, and carry them out to solve the problems they found at first. Then finally, they were able to solve the problems. While repeating this kind of activities they have been able to experience the scientists' research process. Also, they showed a positive attitude toward the scientists' research by understanding problem-solving type research process. These problem-solving type inquiry learning programs seem to have positive effects on students in designing experiments and offering the opportunity for critical argumentation on the causes of the phenomena. The results of comparing pre/post scores for DCT revealed that almost every student has improved his/her ability to design experiments. Students who were accustomed to following teacher's instructions have had difficulty in designing the experiments for themselves at the beginning of the class, but gradually, they become used to doing it through the class and finally were able to do it systematically.

Adherence-induced gene expression in human alveolar macrophages (표면부착에 의한 사람 폐포대식세포의 유전자 발현에 관한 연구)

  • Chung, Man Pyo;Yoo, Chul Gyu;Han, Sung Koo;Shim, Young-Soo;Rhee, Chong H.;Han, Yang Chol;Kim, Young Whan
    • Tuberculosis and Respiratory Diseases
    • /
    • v.43 no.6
    • /
    • pp.936-944
    • /
    • 1996
  • Background: Neutrophils or monocytes separated in vitro by the adherence to plastic surface are known to be activated by surface adherence itself and subsequent experimental data might be altered by surface adherence. Adhesion molecules and gene transcription of the inflammatory mediators are known to be associated in this process. To evaluate whether adhesion molecule and transcriptional activation of the inflammatory substances are also involved in the activation of human alveolar macrophage by the adherence procedure, we designed this experiment. Method : Bronchoalveolar lavage was performed in the person whose lung of either side was confirmed to be nonnal by chest cr and alveolar macrophage was harvested. To measure the expression of Interleukin-8(IL-8) mRNA, manganese superoxide dismutase(SOD) mRNA and CD11/CD18 mRNA in human alveolar macrophage of both adherence state and suspension state, Northern blot analysis was done at 0, 2, 4, 8 and 24hrs after the adherence to plastic surface and during suspension state. Then, phorbol myristate acetate(pMA) and N-formyl-methionyl-leucyl-phenylalanine(fMLP) were added respectively in the same experimental condition. Result : 1) Human alveolar macrophages in the adherent state induced IL-8 mRNA and SOD mRNA expression which was maximal at 8 hours after the adherence to plastic surface. But we could not observe the upregulation of CD18 mRNA by surface adherence. 2) PMA induced these mRNA expression both in the adherent cell and the nonadherem cells, but the induction of mRNA expression by fMLP occurred only in the adherent cells. Conclusion: These results suggest that adherence of huamn alveolar macropahge is an important cell-activating event that may play a critical role in the modulation of lung inflammatory respones.

  • PDF

EFFECT OF NERVE GROWTH FACTOR GENE INJECTION ON THE NERVE REGENERATION IN RAT LINGUAL NERVE CRUSH-INJURY MODEL (백서 설신경 압박손상모델에서 신경성장인자 유전자 주입이 신경재생에 미치는 영향)

  • Gao, En-Feng;Chung, Hun-Jong;Ahn, Kang-Min;Kim, Soung-Min;Kim, Yun-Hee;Jahng, Jeong-Won;Lee, Jong-Ho
    • Maxillofacial Plastic and Reconstructive Surgery
    • /
    • v.28 no.5
    • /
    • pp.375-395
    • /
    • 2006
  • Purpose: Lingual nerve (LN) damage may be caused by either tumor resection or injury such as wisdom tooth extraction, Although autologous nerve graft is sometimes used to repair the damaged nerve, it has the disadvantage of necessity of another operation for nerve harvesting. Moreover, the results of nerve grafting is not satisfactory. The nerve growth factor (NGF) is well-known to play a critical role in peripheral nerve regeneration and its local delivery to the injured nerve has been continuously tried to enhance nerve regeneration. However, its application has limitations like repeated administration due to short half life of 30 minutes and an in vivo delivery model must allow for direct and local delivery. The aim of this study was to construct a well-functioning $rhNGF-{\beta}$ adenovirus for the ultimate development of improved method to promote peripheral nerve regeneration with enhanced and extended secretion of hNGF from the injured nerve by injecting $rhNGF-{\beta}$ gene directly into crush-injured LN in rat model. Materials and Methods: $hNGF-{\beta}$ gene was prepared from fetal brain cDNA library and cloned into E1/E3 deleted adenoviral vector which contains green fluorescence protein (GFP) gene as a reporter. After large scale production and purification of $rhNGF-{\beta}$ adenovirus, transfection efficiency and its expression at various cells (primary cultured Schwann cells, HEK293 cells, Schwann cell lines, NIH3T3 and CRH cells) were evaluated by fluorescent microscopy, RT-PCR, ELISA, immunocytochemistry. Furthermore, the function of rhNGF-beta, which was secreted from various cells infected with $rhNGF-{\beta}$ adenovirus, was evaluated using neuritogenesis of PC-12 cells. For in vivo evaluation of efficacy of $rhNGF-{\beta}$ adenovirus, the LNs of 8-week old rats were exposed and crush-injured with a small hemostat for 10 seconds. After the injury, $rhNGF-{\beta}$ adenovirus($2{\mu}l,\;1.5{\times}10^{11}pfu$) or saline was administered into the crushed site in the experimental (n=24) and the control group (n=24), respectively. Sham operation of another group of rats (n=9) was performed without administration of either saline or adenovirus. The taste recovery and the change of fungiform papilla were studied at 1, 2, 3 and 4 weeks. Each of the 6 animals was tested with different solutions (0.1M NaCl, 0.1M sucrose, 0.01M QHCl, or 0.01M HCl) by two-bottle test paradigm and the number of papilla was counted using SEM picture of tongue dorsum. LN was explored at the same interval as taste study and evaluated electro-physiologically (peak voltage and nerve conduction velocity) and histomorphometrically (axon count, myelin thickness). Results: The recombinant adenovirus vector carrying $rhNGF-{\beta}$ was constructed and confirmed by restriction endonuclease analysis and DNA sequence analysis. GFP expression was observed in 90% of $rhNGF-{\beta}$ adenovirus infected cells compared with uninfected cells. Total mRNA isolated from $rhNGF-{\beta}$ adenovirus infected cells showed strong RT-PCR band, however uninfected or LacZ recombinant adenovirus infected cells did not. NGF quantification by ELISA showed a maximal release of $18865.4{\pm}310.9pg/ml$ NGF at the 4th day and stably continued till 14 days by $rhNGF-{\beta}$ adenovirus infected Schwann cells. PC-12 cells exposed to media with $rhNGF-{\beta}$ adenovirus infected Schwann cell revealed at the same level of neurite-extension as the commercial NGF did. $rhNGF-{\beta}$ adenovirus injected experimental groups in comparison to the control group exhibited different taste preference ratio. Salty, sweet and sour taste preference ratio were significantly different after 2 weeks from the beginning of the experiment, which were similar to the sham group, but not to the control group.

Studies on the Characteristics of Germination and Emergence of Tall Panicum(Panicum dichotomiflorum Michx.) (미국개기장(Panicum dichotomiflorum)의 발아(發芽) 및 출아(出芽) 특성(特性)에 관한 연구(硏究))

  • Kim, M.;Shim, S.I.;Lee, S.G.;Kang, B.H.
    • Korean Journal of Weed Science
    • /
    • v.18 no.2
    • /
    • pp.146-153
    • /
    • 1998
  • This experiment was conducted to clarify the characteristics of germination and emergence of Panicum dichotomiflorum which is a noxious weed species in direct-seeded rice field. P. dichotomiflorum was planted with several treatments such as different depths of irrigation to verifiy the ecological habits of seedling emergence and growth. In order to know the germination characteristics, Panicum dichotomiflorum seeds, pretreated with low-temperature($4^{\circ}C$) stratification for breaking the dormancy, were germinated under different temperature regimes and water potentials. Germination rates of P. dichotomiflorum was increased from 0% of dormant seed to 1%, 35% and 44% by stratification for 21, 28 and 42 days, respectively. Two dominant weed species in directseeded rice fields, Echinochloa crus-galli and Panicum dichotomitlorum, showed different germination habit under different temperature regimes. Echinochloa crus-galli showed more higher germination rate than Panicum dichotomiflorum at relatively low temperature regime(20/$10^{\circ}C$). Both species germinated faster at 30/$20^{\circ}C$ than at 20/$10^{\circ}C$. When the water potential was lowered, germination of Panicum dichotomiflorum was reduced more drastically than Echinochloa crus-galli. The critical water potential for germination of P. dichotomiflorum was -0.7MPa but Echinochloa crus-galli was affected slightly by the same water potential. The results showed that Echinochloa crus-galli can germinate under more wide range of soil water potential than Panicum dichotomiflorum. Emergence of P. dichotomiflorum was highly affected by irrigation depth and the level of water table. When the depth of irrigation water was increased, emergence of P. dichotomiflorum was dragged and emergence rate showed significant difference under the irrigation depth deeper than 6cm. The maximum depth of irrigation water for survival of Panicum dichotomiflorum seedling was 9cm.

  • PDF

Effects of Transaction Characteristics on Distributive Justice and Purchase Intention in the Social Commerce (소셜커머스에서 거래의 특성이 분배적 정의와 거래 의도에 미치는 영향)

  • Bang, Youngsok;Lee, Dong-Joo
    • Asia pacific journal of information systems
    • /
    • v.23 no.2
    • /
    • pp.1-20
    • /
    • 2013
  • Social commerce has been gaining explosive popularity, with typical examples of the model such as Groupon and Level Up. Both local business owners and consumers can benefit from this new e-commerce model. Local business owners have a chance to access potential customers and promote their products in a way that could not have otherwise been easily possible, and consumers can enjoy discounted offerings. However, questions have been increasingly raised about the value and future of the social commerce model. A recent survey shows that about a third of 324 business owners who ran a daily-deal promotion in Groupon went behind. Furthermore, more than half of the surveyed merchants did not express enthusiasm about running the promotion again. The same goes for the case in Korea, where more than half of the surveyed clients reported no significant change or even decrease in profits compared to before the use of social commerce model. Why do local business owners fail to exploit the benefits from the promotions and advertisements through the social commerce model and to make profits? Without answering this question, the model would fall under suspicion and even its sustainability might be challenged. This study aims to look into problems in the current social commerce transactions and provide implications for the social commerce model, so that the model would get a foothold for next growth. Drawing on justice theory, this study develops theoretical arguments for the effects of transaction characteristics on consumers' distributive justice and purchase intention in the social commerce. Specifically, this study focuses on two characteristics of social commerce transactions-the discount rate and the purchase rate of products-and investigates their effects on consumers' perception of distributive justice for discounted transactions in the social commerce and their perception of distributive justice for regular-priced transactions. This study also examines the relationship between distributive justice and purchase intention. We conducted an online experiment and gathered data from 115 participants to test the hypotheses. Each participant was randomly assigned to one of nine manipulated scenarios of social commerce transactions, which were generated based on the combination of three levels of purchase rate (high, medium, and low) and three levels of discount rate (high, medium, and low). We conducted MANOVA and post-hoc ANOVA to test hypotheses about the relationships between the transaction characteristics (purchase rate and discount rate) and distributive justice for each of the discounted transaction and the regular-priced transaction. We also employed a PLS analysis to test relations between distributive justice and purchase intentions. Analysis results show that a higher discount rate increases distributive justice for the discounted transaction but decreases distributive justice for the regular-priced transaction. This, coupled with the result that distributive justice for each type of transaction has a positive effect on the corresponding purchase intention, implies that a large discount in the social commerce may be helpful for attracting consumers, but harmful to the business after the promotion. However, further examination reveals curvilinear effects of the discount rate on both types of distributive justice. Specifically, we find distributive justice for the discounted transaction increases concavely as the discount rate increases while distributive justice for the regular-priced transaction decreases concavely with the dscount rate. This implies that there exists an appropriate discount rate which could promote the discounted transaction while not hurting future business of regular-priced transactions. Next, the purchase rate is found to be a critical factor that facilitates the regular-priced transaction. It has a convexly positive influence on distributive justice for the transaction. Therefore, an increase of the rate beyond some threshold would lead to a substantial level of distributive justice for the regular-priced transaction, threrby boosting future transactions. This implies that social commerce firms and sellers should employ various non-price stimuli to promote the purchase rate. Finally, we find no significant relationship between the purchase rate and distributive justice for the discounted transaction. Based on the above results, we provide several implications with future research directions.

  • PDF

Climate-related Changes in Fruit Growth of 'Fuyu' Persimmon during the Harvest Season (수확기 동안의 기상 변화에 따른 '부유' 감의 과실 생장)

  • Choi, Seong-Tae;Park, Doo-Sang;Son, Ji-Young;Park, Yeo-Ok;Hong, Kwang-Pyo;Cho, Kwang-Sik
    • Horticultural Science & Technology
    • /
    • v.31 no.1
    • /
    • pp.32-37
    • /
    • 2013
  • Relationships among climate changes, early frost, and fruit growth were studied during the final month to harvest of late-maturing 'Fuyu' persimmon (Diospyros kaki) to assess the changes in fruit characteristics during this critical period. The heavy frost on Nov. 16 defoliated more than 70% of the leaves, but with little damage on the fruits. However, all the leaves were defoliated by the heavy frost on Nov. 20, and all the fruits were cold-damaged by $-3.3^{\circ}C$ on Nov. 21. Fruit weight increased by 8-25 g per week from Oct. 25 (142 days after full bloom) to Nov. 15, reaching to 250 g, but it decreased by 3-4 g per week after the frost. Hunter a value of fruit skin gradually increased until the last harvest on Nov. 29 with a temporary halt in early Nov. when temperature was high, whereas fruit firmness rapidly decreased after the frost on Nov. 21. Fruit soluble solids were $15.7-16.1^{\circ}Brix$ for the final month. When some branches were covered with non-woven fabrics to avoid direct contact with frost, the fruits on the branches were not visually damaged by the low temperature although 40-60% of their leaves were defoliated on Nov. 16. However, low temperature on Nov. 20 and 21 defoliated all the leaves, causing cold damage on the fruits. There was a highly significant correlation between the fruit diameter and its weight ($R^2$ = 0.73-0.91). So, the regression equations could be used to estimate weight from diameter of the fruits sampled from the branches with the non-woven fabrics. The calculated fruit weight reached to a maximum of 240 g on Nov. 15. Daily increases in fruit weight were 1.1-2.5 g from Oct. 25 to 31, 1.9-3.5 g from Oct. 31 to Nov. 7, and 1.4-1.6 g from Nov. 7 to 12. However, fruit weight decreased by 0.3-1 g per day after the cold damage on Nov. 21. The results indicate that the most appropriate harvest time could be dependent on relationship of fruit growth to climate.

Economic Analysis, Growth and Pests of Wheat (Triticum aestivum L.) in Gelatin·Chitin Microorganisms-treated Organic Culture (젤라틴·키틴분해미생물을 이용한 밀 유기재배와 관행재배의 생육, 병해충 발생조사 및 경제성 분석)

  • Ahn, Philip;Lee, Jiho;Cha, Kwang-Hong;Seo, Dong-Jun;An, Kyu-Nam;Yoon, Chang-Yong;Kim, Kil-Yong;Jung, Woo-Jin
    • Korean Journal of Organic Agriculture
    • /
    • v.29 no.2
    • /
    • pp.223-240
    • /
    • 2021
  • This study was carried out to investigate the economic value of organic wheat production using gelatin·chitin microorganisms in Gwangsan-gu, Gwangju city. The soil condition of experiment field was clay loam Jisan series. The organically cultivated fields were sprayed gelatin and chitin degrading bacteria. The test was performed at conventionally cultivated field and organically cultivated field. Emergence of weed on organically cultivated field was significantly higher than conventionally cultivated field which sprayed herbicide before seeding. Weed emergence have a critical impact on grain yield. Occurrence of diseases and insect pests were higher than conventionally cultivated fields. In 2019, the amount of lodging in conventionally cultivated field were higher than conventionally cultivated field. In 2020, lodging and wet injury were occur in both field. Comparing yield element between organically and conventionally cultivated experimental area, grain yield in organically cultivated field was shown slightly higher amount than conventionally cultivated field. However in the actual yield of 2019, organically cultivated field shows 20% deceased yield because of overgrown weed. In 2020, weed emergence and yellow mosaic virus by wet injury cause 30% decease in the grain yield in organically cultivated field. Content of protein, carbohydrates, ash, water and fat in the grain were not different significance. In 2019, net incomes of conventionally cultivated wheat was 461,031 won/0.1 ha while organically cultivated wheat was 443,437 won/0.1 ha. In the rate of income, conventionally cultivated field was 83.0% as against organically cultivated field (73.3%). In 2020, net incomes of organically cultivated wheat was 437,812 won/0.1 ha while conventionally cultivated wheat was 418,281 won/0.1 ha. In the rate of income, conventionally cultivated field was 81.6% as against organically cultivated field (73.0%).

Study on Control of Thermal Environmental Factors for Improvement of Productivity of Laying Hens in Summer (여름철 산란계사 내 열환경인자 중 제어요소에 관한 연구)

  • Kim, Seong-Wan;Lee, Tae-Hoon;Cha, Gwang-Jun;Gutierrez, Winson M.;Chang, Hong-Hee
    • Journal of agriculture & life science
    • /
    • v.53 no.2
    • /
    • pp.121-129
    • /
    • 2019
  • This study carried out to determine control factors for the improvement of productivity of laying hens suffering heat stress during hot weather. A total of 48,451 ISA Brown layers were housed in a farm located in Gyeongsangnam-do, Republic of Korea. Five thermo-hydrometer loggers were installed inside the house to collect data of dry-bulb temperature and relative humidity. The experiment continued for 81 days when the summer season begins from 19th June to 7th September, 2018. This study analyzed the correlations among layers' production index and daily average, highest, and lowest temperature; daily average, highest, and lowest relative humidity; and daily average, minimum, and maximum THI. The result indicated that feed consumption, hen-day egg production, egg weight, and FCR decreased as the daily average, highest and lowest dry-bulb temperature and THI rise (p<0.01). On the other hand, water intake increased as the daily average, highest and lowest dry-bulb temperature and THI rise (p<0.001). The relative humidity was not considered to have direct correlations to the layers' production index (p>0.05). However, it was noticeable that the mortality did not have significant relations with daily average and highest temperature; THI; or daily average, highest and lowest relative humidity while it was relevant to the daily lowest temperature and THI (p<0.05). In conclusion, to enhance the productivity of laying hens in a hot climate, it is recommended that daily average, highest, and lowest dry-bulb temperature and THI are maintained as low as possible. Especially, the daily lowest temperature is needed to lower to 20℃, which is the lowest critical temperature for layers.

A Comparison between the Reference Evapotranspiration Products for Croplands in Korea: Case Study of 2016-2019 (우리나라 농지의 기준증발산 격자자료 비교평가: 2016-2019년의 사례연구)

  • Kim, Seoyeon;Jeong, Yemin;Cho, Subin;Youn, Youjeong;Kim, Nari;Lee, Yangwon
    • Korean Journal of Remote Sensing
    • /
    • v.36 no.6_1
    • /
    • pp.1465-1483
    • /
    • 2020
  • Evapotranspiration is a concept that includes the evaporation from soil and the transpiration from the plant leaf. It is an essential factor for monitoring water balance, drought, crop growth, and climate change. Actual evapotranspiration (AET) corresponds to the consumption of water from the land surface and the necessary amount of water for the land surface. Because the AET is derived from multiplying the crop coefficient by the reference evapotranspiration (ET0), an accurate calculation of the ET0 is required for the AET. To date, many efforts have been made for gridded ET0 to provide multiple products now. This study presents a comparison between the ET0 products such as FAO56-PM, LDAPS, PKNU-NMSC, and MODIS to find out which one is more suitable for the local-scale hydrological and agricultural applications in Korea, where the heterogeneity of the land surface is critical. In the experiment for the period between 2016 and 2019, the daily and 8-day products were compared with the in-situ observations by KMA. The analyses according to the station, year, month, and time-series showed that the PKNU-NMSC product with a successful optimization for Korea was superior to the others, yielding stable accuracy irrespective of space and time. Also, this paper showed the intrinsic characteristics of the FAO56-PM, LDAPS, and MODIS ET0 products that could be informative for other researchers.