• Title/Summary/Keyword: critical experiment

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An Action-Generation Method of Virtual Characters using Programming by Demonstration (Programming by Demonstration을 이용한 가상 캐릭터의 행동 생성 기법)

  • Sung, Yun-Sick;Cho, Kyung-Eun;Um, Ky-Hyun
    • Journal of Korea Game Society
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    • v.11 no.2
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    • pp.141-149
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    • 2011
  • The substantial effort is required to make a virtual character smoothly move like a human being in the virtual environment. Since a virtual character acts on the basis of the actions, it is the most critical to define actions for smooth flow of action. It has been actively studied the programming by demonstration which defines series of actions to be performed by a virtual character based on the actions operated by a person. However, such approaches can't easily draw many sequential actions because they create sequential actions in the same length all the time or restrict the actions used to create such actions. For smooth flow of action, it is required to derive sequential actions as much as possible from the actions of a virtual character and to select the representative set of actions. Accordingly, it is necessary to study how to create sequential actions as reducing diverse limits. This study suggests the approach to select sequential actions suitable for a virtual character by collecting the actions of a character manipulated by a person and deriving a set of actions to be performed by a virtual character. The experiment describes the process to create the actions by applying the approach suggested in this study to the driving game. In accordance with the analysis results, it was found that a set of actions performed by a person was generated without being restricted by a length or a part to divide. Finally, we confirmed that the suggested method selects the best sequential actions, appropriate to virtual character, among more generated actions.

Optimization of monitoring methods for air-borne bacteria in the environmental conditions of pig facilities (무균 돈사 환경 모니터링을 위한 대기 중 미생물 탐지기법 확립)

  • Lee, Deok-Yong;Seo, Yeon-Soo;Kang, Sang-Gyun;Yoo, Han Sang
    • Korean Journal of Veterinary Research
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    • v.46 no.3
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    • pp.255-261
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    • 2006
  • Experimental animals have been used to biological and medical purposes and the animals must be, for these purposes, healthy and clean to microbial infection. However, the animals can be easily exposed to pathogenic microorganism via several routes. Of the routes, environmental conditions are the most important factors to keep the animals healthy and clean, especially air condition. Monitoring of air-condition has been required to keep the animal healthy and clean. However, any guideline is not available for experimental conditions with pigs. Therefore, the sampling times and points were compared in different conditions to establish an optimal protocol for monitoring of air borne bacteria. Tryptic soy agar(TSA), blood agar containing 5% defibrinated sheep blood and Sabraud dextrose agar(SDA) were used as media to capture total bacteria, pathogenic bacteria and fungi, respectively. Two methods, compulsive capture using an air-sampler and capturing fall-down bacteria were used to capture the microorganisms in the air. The points and time of capturing were different at each experiment. Air borne microorganisms were captured at three and five points in the open and closed equipments, respectively. Air was collected using an air-sampler for 1 min and 5 min and the agar plates as open status were left from 30 min to 2hr. At first, we monitored an experimental laboratory which dealt with several pathogenic bacteria and then, a protocol obtained from the investigation was applied to open or close experimental conditions with pigs. Number of bacteria was high from 10:00 to 15:00, especially on 13:30-15:30 but sharply decreased after 17:00. The tendency of the number of bacteria was similar between two methods even though the absolute number was higher with air sampler. Critical difference in the number of cells was observed at 5 min with air sampler and 2 hr with fall-down capturing method. However, 1 min with air sampler and 1 hr with fall-down capturing were the best condition to identify bacterial species collected from the air. Number of bacteria were different depending on the sampling points in closed condition but not in opened condition. Based on our results, a guide-line was suggested for screening air-borne microorganism in the experimental conditions with pigs.

Analysis of Nitrosation Inhibition and Antioxidant Effect by Phyto-Extract Mixture (식물추출 혼합제재인 phyto-extract mixture의 니트로세이션 억제능과 항산화능 분석)

  • Kim, Ji-Hoon;Shin, Mi-Jung;Cho, Hee-Jae;Lee, Sang-Won;Jeong, Jong-Moon
    • Korean Journal of Food Science and Technology
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    • v.33 no.6
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    • pp.656-663
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    • 2001
  • The most representative nitrosamine derived from nicotine, nitrosamine-4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone(NNK), has been reported to cause lung cancer in A/J mice. It has been also demonstrated that NNK-induced lung tumorigenesis involves $O^6-methylguanine(O^6MeG)$ formation, leading to $GC\;{\rightarrow}\;AT$ transitional mispairing during DNA replication. Our in vitro experiment, modified from the method of DBA assay, examined the ability of phyto-extract mixture to inhibit the metabolism of nicotine to nitrosamines. The production of nitromorpholine from morpholine was inhibited about 75% at the concentration of 20 mg/mL of phyto-extract mixture, which was lower than vitamine C and green tea powder. NNK, which is a pro-carcinogen in laboratory animals, is hydroxylated primarily in liver and lung by CYP 1A2, 2A6 and 3A4. A critical phase. of NNK activation is its change to an unstable metabolite methyl-diazohydroxide via CYP-mediated ${\alpha}-hydroxylation$; and then it provides a methyl group to the DNA to form DNA adducts which can easily induce mutations. $Aroclor^R$ 1254 was used to induce CYPs in the liver of a Sprague-Dawley rat. The ability of various test samples to inhibit CYPs that participate in NNK activation was evaluated, following the removal of the liver from the rat. Microsomal CYPlA2 catalyzing the conversion of NNK into strong carcinogenic chemicals was inhibited more efficiently by phyto-extract mixture than green tea powder. These results indicate that phyto-extract mixture can be used to reduce $O^6MeG$ DNA adducts for chemoprevention.

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Measurement of Plasma Parameters (Te and Ne) and Reactive Oxygen Species in Nonthermal Bioplasma Operating at Atmospheric Pressure

  • Choi, Eun Ha;Kim, Yong Hee;Kwon, Gi Chung;Choi, Jin Joo;Cho, Guang Sup;Uhm, Han Sup;Kim, Doyoung;Han, Yong Gyu;Suanpoot, Pradoong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.141-141
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    • 2013
  • We have generated the needle-typed nonthermal plasma jet by using an Ar gas flow at atmospheric pressure. Diagnostics of electron temperature anddensity is critical factors in optimization of the atmospheric plasma jet source in accordance with the gas flow rate. We have investigated the electron temperature and density of plasma jet by selecting the four metastable Ar emission lines based on the atmospheric collisional radiative model and radial profile characteristics of current density, respectively. The averaged electron temperature and electron density for this plasma jet are found to be ~1.6 eV and ~$3.2{\times}10^{12}cm^{-3}$, respectively, in this experiment. The densities of OH radical species inside the various bio-solutions are found to be higher by about 4~9 times than those on the surface when the argon bioplasma jet has been bombarded onto the bio-solution surface. The densities of the OH radicalspecies inside the DI water, DMEM, and PBS are measured to be about $4.3{\times}10^{16}cm^{-3}$, $2.2{\times}10^{16}cm^{-3}$, and $2.1{\times}10^{16}cm^{-3}$, respectively, at 2 mm downstream from the surface under optimized Ar gas flow 250 sccm.

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Permeation Properties of Single Gases ($N_2$, $O_2$, $SF_6$, $CF_4$) through PDMS and PEBAX Membranes (PDMS와 PEBAX 분리막을 통한 단일기체($N_2$, $O_2$, $SF_6$, $CF_4$) 투과 특성)

  • Kim, Hanbyul;Lee, Minwoo;Park, Wankeun;Lee, Soonjae;Lee, Hyunkyung;Lee, Sanghyup
    • Membrane Journal
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    • v.22 no.3
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    • pp.201-207
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    • 2012
  • In this study, we investigated permeation of single gas ($N_2$, $O_2$, $CF_4$, and $SF_6$) through flat sheet membrane composed of PDMS (poly-dimethylsiloxane) and PEBAX (polyether block amides). Gas permeation experiment was performed with various feed pressure. Permeability was estimated using permeation flux measured by continuous-flow technique. The permeability of gases except $SF_6$ in PDMS were decreased with the upstream pressure increased. $SF_6$ is much more permeable than $CF_4$, which is due to higher critical temperature of $SF_6$. The permeability decreased in the following order: $O_2$ > $N_2$ > $SF_6$ > $CF_4$. On the other hand, the permeability of gases in PEBAX followed the order: $O_2$ > $N_2$ > $CF_4$ > $SF_6$ which are opposite of the order of kinematic diameter (${\AA}$)($SF_6$ > $CF_4$ > $N_2$ > $O_2$). The $SF_6/CF_4$ pure gas selectivity in PDMS was 2.1 at 0.7 MPa.

Cultural and Physiological Conditions for T-2 Toxin Production by Fusarium sp. (Fusarium 균주의 배양 조건 및 생리적 조건에 따른 T-2 toxin의 생성 조건)

  • 홍성희;양규환
    • Korean Journal of Microbiology
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    • v.36 no.2
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    • pp.91-96
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    • 2000
  • The cultural and physiological conditions for the T-2 toxin [4,15-diacetoxy-8-(3-mety1butyloxy)-12,13- epoxy-trichothec-9-en-3-01, $C_{24}H_{30}O_9$] production by Fusarium spp. were studied. Thin layer chromatography (TLC) assay and the microbiological assay uslng Rhodotomla rubra were used to quantitate tbe T- 2 toxin. Among the four strains of Fusarium spp., F tn'cinctum NRRL 3299 was best for T-2 toxin production. In solid culture, white com grit medium was best for T-2 toxm production. Temperature played a critical role in the production of T-2 toxin. T-2 toxin production was favored by long duration of low-temperature incubation. The growth and toxin production were relatively high on galactose, fructose, glucose, and sucrose media, when each was used as a sole carbon source, and relatively low on sorbitol, glycerol, and lactose media. For nitrogen sources, $NH_4^(+) and NO_3^{-}were used well as a sole nitrogen source, but $NO_2^-$ was not used. Initial pH and speed of shaker also affected the production of T-2 toxin. From temperature shifting experiment, it is clear that T-2 toxin metabolic pathway is regulated by temperature-dependent enzyme depression or enzyme induction system.

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Evaluation on Mechanical Properties of High Strength Light-Weight Concrete with Elevated Temperature and loading (하중조건과 고온에 의한 고강도 경량 콘크리트의 역학적 특성 평가)

  • Kim, Gyu-Yong;Kim, Young-Sun;Choe, Gyeong-Cheol;Park, Hyun-Gil;Lee, Tae-Gyu
    • Journal of the Korea Concrete Institute
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    • v.23 no.6
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    • pp.723-730
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    • 2011
  • It is very important to experimentally evaluate concrete behavior at elevated temperature because aggregates make up approximately 80 percent of volume in concrete. In this study, an experiment to evaluate mechanical properties of normal weight and light weight concrete of 60 MPa was conducted. Based on loading level of 0, 20 and 40 percent, the tests of 28 days compressive strength, elastic modulus, thermal strain, total strain, and transient creep using ${\phi}100{\times}200mm$ cylindrical specimens at elevated temperature were performed. Then, the results were compared with CEB (Committes Euro-international du Beton) model code. The results showed that thermal strain of light weight concrete was smaller than normal weight concrete. Also, the results showed that compressive strength of light concrete at $700^{\circ}C$ was higher than normal weight concrete and CEB code, similar to that obtained at ambient temperature. Transient creep developed from loading at a critical temperature of $500^{\circ}C$ caused the concrete strains to change from expansion to compression. The transient creep test result showed that internal force was high when the ratio of shrinkage between concrete and aggregate was more influential than thermal expansion.

The Durability of the Concrete Using Bottom Ash as Fine Aggregate (바텀애시를 잔골재로 사용한 콘크리트의 내구성능에 관한 연구)

  • Park, Seung-Ho;Lee, Jeong-Bae;Kim, Seong-Soo
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.4 no.4
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    • pp.349-355
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    • 2016
  • This study is about the reuse of bottom ash, which is released as a necessity in thermal power plant. In general, coal-ash are classified as fly-ash, bottom-ash, cinder-ash. Of these, a large amount of fly ash is being recycled as cement substitutes. While, recycling rates of bottom ash are the lowest due to its porosity and high absorption. In this study, the durability of the concrete using bottom ash as a concrete fine aggregate was evaluated. The using level of the bottom ash ranges to step-by-step from 0% to 30%. According to the result of the durability test, regardless of the presence of the bottom ash, freeze-thaw durability could be secured by air entrainment. In case of the resistance to chloride ions penetration, the length change, and the effects on heavy metals, the replacement of bottom ash as fine aggregate was not critical. Although carbonation penetration was higher as the replacement level of bottom ash increased, the experiment showed that it could be possible to use bottom ash as concrete fine aggregate with proper mix design.

Cariogenic Potential of Nutritional Supplements for Children on Bovine Teeth (Bovine teeth에 대한 어린이용 영양제의 우식유발능)

  • Heo, Narang;Lee, Kwanghee;An, Soyoun;Song, Jihyun;Ra, Jiyoung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.41 no.3
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    • pp.233-240
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    • 2014
  • An in vitro study on cariogenic potential of four over-the-counter nutritional supplements for children was performed. The experimental groups were four over-the-counter nutritional supplements. The positive control group was 10% sucrose solution (S), and the negative control group was artificial saliva (T). The pH of each group, the buffering capacities, acid production, the microhardness changes of the bovine teeth specimens were measured. The pH of all experimental groups were lower than critical pH 5.5 where enamel demineralization starts. The buffering capacity of the Hama Vitamin Pharm (Hamsoa Pharm Co., Korea) was highest, and the Smart Chewable Vitamin A (JW Pharm Co., Korea) had the lowest buffering capacity. The reduction rates of the pH of the experimental groups were significantly higher than that of the negative control group (p < 0.05). The microhardness of enamel of all experimental groups and the positive control group significantly decreased. In contrast, the microhardness of enamel of the negative control group significantly increased after experiment (p < 0.05). The reduction rate of the microhardness of enamel of the Hama Vitamin Pharm (Hamsoa Pharm Co., Korea) was significantly higher and Hikid Plus (Sanga Pharm Co., Korea) was significantly lower than the other experimental groups.

Measurement of an Isoelectric Point and Softness of a EO-PO Adducted Zwitterionic Surfactant (EO-PO가 부가된 양쪽성 계면활성제의 등전점 및 유연력 측정에 관한 연구)

  • Lim, JongChoo;Mo, DaHee;Lee, JinSun;Park, JunSeok;Han, DongSung
    • Korean Chemical Engineering Research
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    • v.50 no.3
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    • pp.455-463
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    • 2012
  • In this study, the measurement of physical properties of ethylene oxide-propylene oxide adducted zwitterionic surfactants were measured such as critical micelle concentration, surface tension, interfacial tension, contact angle, viscosity and foam stability. Also, the dual function characteristics of a zwitterionic surfactant were investigated by determining an isoelectric point, which were obtained using zeta potential measurement and QCM (quartz crystal microbalance) experiments. The isoelectric point of DEP-OSA82-AO zwitterionic surfactant determined by zeta potential measurement was close to that obtained by QCM experiment and both results have shown almost the same trend as that determined by the frictional property measured using an automated mildness tester. In particular, it has been observed that DEP32-OSA82-AO and DEP34-OSA82-AO surfactants provide better softening effect at a pH of acidic or neutral condition than at an alkaline condition. This result indicates that both surfactants act as a cationic surfactant at a pH of acidic or neutral condition and thus provide good softening effect during a rinsing cycle in the detergency process.