• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.2
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• pp.137-144
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• 2004

The objective of this experiment was to suggest the optimum N fertilization level for turf type mixtures {Kentucky bluegrass(Newport) 40% + tall fescue(Rebell Jr.) 20%. + perennial ryegrass(Palmer II) 10% + redtop(Barricuda) 10% + red fescue(Salem) 10% + creeping bentgrass(Crenshaw) 10%}. The field trials were conducted from 1998 to 2000 at Chungnam National University in order to evaluate the dry matter yield and quality on the N levels(100, 150 and 200kg/ha). With increasing the N level, the average DM yields over 2-years of turf type mixtures significantly increased. The average total DM yields were obtained 6,551, 8,182 and 9,501kg/ha at N 100, 150 and 200kg/ha level(p<0.05), respectively. CP content and DMD were increased, whereas fibrous constituents was decreased(p<0.05), but CPDM and DDM yields were significantly increased with increasing the N levels over 2-year(p<0.05). Based on the results, considering the total DM yield and the quality of herbages which were incidentally produced from turf type mixtures, it can be suggested that the suitable N level was 200kg/ha. But increased N fertilizer usage for general purpose use and expenses on turf type mixtures should be considered in advance.

• Asian Journal of Turfgrass Science
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• v.15 no.4
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• pp.157-168
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• 2001

This study was carried out to investigate the offsets of pyroligneous acid on the growth of ‘Penncross’Creeping Bentgrass (Agrostis palustris Huds.) and to propose the practical use for ecologically responsive management in Korean golf courses. In the plots affected by 300, 500, and 800 times diluted pyroligneous acid, the turfgrass growth increment and the visual quality were measured at fixed periods. The data were subjected to paired samples T-test. The results summarized are as follows； 1) In leaf growth increment, density, and root spread depth, turfgrass affected by 500 and 800 times diluted pyroligneous acid were superior to the control continually. It was reliable in the statistics. Especially, 500 times diluted solutions was superior to the others. 2) In color, texture, uniformity, and recuperative strength, the visual quality of turfgrass affected by 500 and 800 times diluted pyroligneous acid were superior to the control continually. It was reliable in the statistics except the recuperative strength. Espe-cially, 500 times diluted solutions tended to be superior to the others. 3) There was no symptom of a disease in all plots. The existing management was thought to be suitable in this golf course. 500 and 800 times diluted pyroligneous acid were not thought to trigger or promote a disease, 4) After investigating the effect of pyroligneous acid on turfgrass growth, the soils of all plots were analyzed. In all plots, the chemical properties of the soils were not changed obviously. 5) Considering the results mentioned above, the practical use of 500 and 800 times diluted solutions might decrease the amount of nutrients and agricultural chemicals used in golf courses as well as promote turfgrass growth in golf courses.

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.260-267
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• 2016

Amino acids in the plant were intermediate metabolites which produced by uptake and assimilation of nitrogen and these extracts which gained by bio-chemical digestion from protein of plant or animal were a source of functional fertilizer. This study was conducted to evaluate effects of keratin amino acid fertilizer (KAF) gained from animal hair or hoof on changes of turfgrass quality and growth by investigating turf color index, chlorophyll index, shoot number, clipping yield, and nutrient content in the turfgrass tissue. Treatments were designed as follows; non-fertilizer (NF), compound fertilizer (CF), keratin amino acid fertilizer treatments [CF + KAF $0.26ml\;m^{-2}$ (CKF), CF + KAF $0.52ml\;m^{-2}$ (2CKF)], and only keratin amino acid fertilizer treatment (KF). Shoot number, clipping yield, and nitrogen uptake of KF were higher than those of CF. As compared with CF, soil chemical properties, turf color index, chlorophyll index and clipping yield of keratin amino acid fertilizer were not significant, but shoot number and uptake of N and K were increased significantly. These results show that the application of keratin amino acid fertilizer increased shoot number and growth by increased uptake of nitrogen and potassium.

• Asian Journal of Turfgrass Science
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• v.12 no.2
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• pp.113-126
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• 1998

Wear simulator로 답압이 가해진 크리핑 벤트그라스 (Agrostis palustris Huds.) 훼어웨이에서 관수방법·예지물 순환 및 질소시비수준의 여러 가지 잔디관리 요인이 엽조직 및 토양질소 함유량에 미치는 효과를 알아보기 위해 본 연구를 수행하였다. 연구포장은 'Penncross'크리핑 벤트그라스 반디밭으로 1988년에 sharpsburg silty-clay loam (Typic Argiudoll) 토양에 조성하였으며, 실험은 1989년부터 1991년까지 3년간 수행하였다. Split-split-plot 실험디자인을 사용하여 주구에 daily or biweekly irrigation, 세구에 clipping return or removal, 세세구에 low-N (5g), moderate-N (15g), high-N (25g N m-2 yr-1)처리를 난괴법 3 반복으로 배치하였다. 생육기간중 잔디예초는 12mm 예고로 일주일에 4번 실시하였고, 기타 잔디관리는 high maintenance 수준으로 유지되는 한지형 양잔디 훼어웨이 기준으로 실시하였다. 엽조직 및 토양샘플은 1989년 2회, 1990년과 1991년에는 3회씩 채취하여, 네브라스카 주립대 토양식물분석실에서 분석하였다. 답압이 가해진 크리핑 벤트그라스 훼어웨이 잔디에서 엽조직 질소함유량은 여러 가지 잔디관리 방법간 상호작용 효과가 관찰되었다. 1989년 나타난 질소시비수준과 관수방법간의 상호작용에서 daily irrigation 지역의 엽질소 함유량은 질소시비량이 low-N 수준에서 high-N 수준으로 증가함에 따라 3.51%에서 3.94%로 quadratic pattern으로 증가하였다. High-N 처리지역에서 엽질소 함유량은 daily irrigation 관수방법이 biweekly irrigation 관수방법보다 약 4% 정도 더 많은 것으로 나타났다. 엽질소 함유량은 특히 질소시비 수준에 따라 크게 영향을 받는 것으로 나타났다. 1990년 질소시비량이 low-N 수준에서 high-N 수준으로 증가함에 따라 3.50%에서 4.25%로 quadratic pattern으로 증가하였고, 1991년에는 4.20%에서 4.60%까지 linear pattern으로 증가하였다. High-N 처리구의 엽조직 질소함유량은 low-N 처리구와 비교시 1990년에는 21%, 1991년에는 10% 더 많은 것으로 나타났다. 잔디조성후 시간이 경과함에 따라 엽조직의 질소함유량도 증가하였다. Low-N 수준에서 1991년 엽질소 함유량은 1990년에 비해 20% 증가하였으며, high-N 수준에서는 1991년의 엽조직 질소함유량이 1990년 보다 8% 증가한 것으로 나타났다. 따라서, 잔디조성후 경과기간에 따라 연간 시비량을 조절할 필요가 있으며, 특히 새로 조성된 잔디밭과 조성된 지 어느 정도 지난 기존 잔디밭간에 차별화된 관리프로그램이 필요한 것으로 판단되었다. 잔디관리에서 답압이 가중되는 정도에 따라 지역별로 장기간 차별화된 관리 접근을 해야하고, 정기적으로 토양 및 엽분석을 실시해서 시비프로그램에 활용하는 것이 필요하다 하겠다. 본 연구결과 나타난 잔디관리 요인간 상호작용효과는 잔디관리시 여러 가지 관리방법에 따른 효과를 입체적으로 분석해서 해당 골프장 현실에 적합한 통합적인 잔디관리(integrated turfgrass management)의 필요성을 제시한다고 할 수 있겠으며, 또한 답압가중 정도에 따른 잔디관리요인간의 반응효과차이는 향후 무답압 지역에서 실시된 연구결과를 답압을 받고 있는 경기장 및 골프장 등의 잔디밭에 적용할 경우에는 주의깊게 데이터 활용을 해야 되리라고 사료되었다.

• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.163-170
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• 2022

There is a continuous rise in the commercialization of living modified (LM) organisms worldwide. While LM plants have not yet been cultivated in South Korea, research, development, import of products, and registration of related research facilities are progressing. LM plants should be tested in greenhouses and fields during development. Furthermore, environmental risk assessment and safety management should be performed before their release into the environment. Research on LM plant development is conducted in laboratories as well as confined greenhouses and fields. Safety management regulations are provided as combination standards for the LMO Act in each research district. The accidental release of the LM petunia in Japan was a significant incident related to LM plant research. It implies that normal plants within the distance of crossing should be regarded as LM plants. In the United States, LM creeping bentgrass was released into the environment, thus necessitating the establishment of stringent measures to prevent the scattering of LM plant seeds by wind or other mediums. In South Korea, LM Zoysia and LM cotton were released through rainwater. Therefore, safety measures that prevent LM seed mixing and plant vegetative propagules escaping into the environment via rainwater must be established. Preventing the dispersal of unapproved LM plants requires significant time, expenditure, and effort. Researchers should first identify the impact of LM plants on the ecosystem, and steps to avert their environmental release must be implemented.

• Korean Journal of Weed Science
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• v.14 no.4
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• pp.265-271
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• 1994

This study was conducted to determine the herbicidal activity, persistance, downward movement and effect on bentgrass of 7 dinitroaniline herbicides such as benefin [N-butyl-N-ethyl-2,6-dinitro-4-(trifluoromethyl) benzenamine], beslogine [N,N-dibutyl-2,6-dinitro-4-trifluoromethylaniline], prodiamine [2,4-dinitro-$N^3$,$N^3$-dipropyl-6-(trifluoromethyl)-1,3-benzenediamine], pendimethalin [N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzenamine], trifluralin [2,6-dinitro-N,N-dipropyl-4-(trifluoromethyl) benzenamine], ethalfluralin [N-ethyl-N-(2-methyl-2-propenyl)-2,6-dinitro-4-(trifluoromethyl) benzenamine], and oryzalin [4-(diprop-ylamino)-3,5-dinitrobenzene-sulfonamide) together with bensulide [O,O-bis(1-methylethyl) S-[2-[(phenyl-sulfonyl) amino]ethyl]phosphorodithioate] and siduron [N-(2-methylcyclohexyl)-N'-phenylurea) as the control. In addition, distribution of pendimethalin and benefin into bentgrass was also determined. Prodiamine, benefin, and pendimethalin at the 1/16 dose of the recommended rate showed very high herbicidal activity($LD_{90}$) on Digitaria sanguinalis, but ethalfluralin and bensulide showed $LD_{90}$ at the 1/4 dose of the recommended rate, showing difference in herbicidal activities among dinitroaniline herbicides. All the herbicides except for pendimethalin had the lower herbicidal activity in sandy soil than that of clay-loam soil. Benefin, beslogine, prodiamine, oryzalin, bensulide and siduron persisted in the soil for about 50 days, but pendimethalin persisted in the soil for about 35 days, and trifluralin and ethalfluralin for about 25 days. Ethalfluralin, oryzalin and bensulide were the most mobile(downward movement) of the 9 herbicides studied. Less mobility was observed in the turfgrass condition than that of the bare soil. Beslogine bensulide prodiamine and benefin had no injury effect on bentgrass(Agrastis atolonifera L., penncross creeping bent grass). However, herbicides like oryzalin, trifluralin and pendimethalin reduced the dry weight of bentgrass by 12%, 30% and 40%, respectively. No significant difference in distribution of pendimethalin and benefin into inner part of leaves, surface and wax layer of bentgrass was observed, and thus it seems that different phytotoxic effect between pendimethalin and benefin may be attributed to different metabolism and mode of action.

• Weed & Turfgrass Science
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• v.2 no.3
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• pp.306-311
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• 2013

Pythium blight occurred by Pythium aphanidermatum on chewing fescue cv. "Jamestowm II" from early June, 2010 and 2011 at the test field in Daegu University in Gyeongbuk Province, Korea. Disease symptoms on the turfgrass were leaf blights dying from the leaf tip and root rot, which appeared patches of brown to dark brown color or gray brown color in the field. The pathogens (40-1 isolate) of Pythium blight was isolated from the diseased leaf and crown tissue and cultured on potato-dextrose agar (PDA) for identification. Lobulate sporangia were inflated, complex structures, and filamentous sporangia were usually indistinguishable from vegetative hyphae. Sequences of ribosomal RNA gene of the fungus were homologous with similarity of 100% to those of P. aphanidermatum isolates in GenBank database. Pathogenicity was also confirmed on the chewing fescue, creeping betgrass and Kentucky bluegrass by Koch's postulates. This is the first report of Pythium blight on chewing fescue caused by P. aphanidermatum in Korea.

• Asian Journal of Turfgrass Science
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• v.24 no.1
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• pp.50-55
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• 2010

Five turfgrass species and 46 cultivars were investigated for difference of spring greenup and living ground cover. Turf color and Normalized Difference Vegetation Index (NDVI) for greenup were investigated between species and cultivars. Turf color and NDVI were showed significantly different among species and cultivars. Turf color was showed significantly different among 20 cultivars of kentucky bluegrass (Poa pratensis L.). NDVI was significantly difference among 20 cultivars of kentucky bluegrass and 6 cultivars of tall fescue (Festuca arundinacea Schreb). The percentage living ground cover was showed significantly different among creeping bentgrass (Agrostis palustris Huds) cultivars and fineleaf fescue cultivars in spring.

• Korean Journal Plant Pathology
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• v.10 no.1
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• pp.54-60
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• 1994

Incidence of Rhizoctonia blight ranged from 22.2% to 100% in the golf courses at six geographical locations in Korea from 1989 to 1993. Rhizoctonia blight occurred more severly in southern area than in northern area. Fifty seven isolates of Rhizoctonia solani obtained from diseased parts of zoysiagrasses were grouped to AG2-2 by anastomosis test. Pathogenicity testes revealed that this pathogen was strongly pathogenic to Korean lawngrasses(Zoysia japonica, Z. matrella, Z. tenuifolia), but not pathogenic to creeping bentgrass(Agrostis palustris), bermuldagrass(Cynodon dactylon), Kentucky bluegrass(Poa pratensis), perennial ryegrass(Lolium prenne), and creeping red fescue(Festuca rubra subsp. rubra L.). The isolation frequency of R. solani AG2-2 fro sheaths of the infected plants was the highest by 91.67%, and that from stolons and roots was 11.13% and 5.63% respectively. The pathogen was not isolated from the leaves. Population density of R. solani in the lawn of large circular patch was highest on surface soils down to 1 cm deep with the value of 4.9$\times$104 (CFU/g soil), but below 1 cm population density decreased sharply down to 0.8~9.8$\times$103 (CFU/g soil). Horizontal distribution of propagules in turfgrass soil was higher in the margin than in center of patch, where the number of propagules was similar to these of healthy looking soils close to the margin of diseased patch. The meteorological factors influencing the outbreak of the disease were temperature, the number of rainy days and precipitation. Optimum temperature for disease development of Rhizoctonia blight in field was 20~22$^{\circ}C$, and that for hyphal growth of R. solani AG2-2 in vitro was 25~3$0^{\circ}C$. In Pusan area, Rhizoctonia blight first occurred in late April and rapidly developed in late June. The disease slightly decreased during July to August and developed again in late September in 1993. The monthly disease progress in Pusan area was similar to that in Kyeonggi province.

• The Plant Pathology Journal
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• v.16 no.5
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• pp.286-289
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• 2000

Mechanol extracts of three cold-tolerant eucalyptus trees-Eucalyptus darlympleana, E. gunnii and E. unigera were screened for antimicrobial activity against twenty two phyto-pathogenic fungi and six food-borne bacterial pathogens. E. unigera showed the antagonistic activity against all the tested pathogens. Among the tested fungal pathogens, Pythium species were highly sensitive to the leaf extracts. Especially, P. vanterpoolii, a causal agent of leaf blight in creeping bentgrass (Agrostis palustris), was completely inhibited by the extracts. The eucalyptus extracts were also effective in inhibiting the fungal growth of Botrytis cinerea and Phomopsis sp. isolated from the lesions of kiwifruit soft rot during post-harvest storage. Escherichia coli O-157 was less sensitive to the inhibition than the other bacterial pathogens tested. It was likely that Gram positive bacteria-Bacillus subtilis and Streptococcus mutans were more sensitive to the eucalyptus extracts than Gram negative bacteria-Escherichia coli, Salmonella enteritidis and Pseudomonas aeruginosa. Our findings suggest that the cold-tolerant eucalyptus species have antimicrobial properties that can serve the development of novel fungitoxic agents or food preservatives.