• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.6
• /
• pp.801-806
• /
• 2016

The objectives of this study were to evaluate the effects of different supplemental levels of Saccharomyces cerevisiae fermentation product (SCFP; Original XP; Diamond V) on lactation performance in Holstein dairy cows under heat stress. Eighty-one multiparous Holstein dairy cows were divided into 27 blocks of 3 cows each based on milk yield ($23.6{\pm}0.20kg/d$), parity ($2.88{\pm}0.91$) and day in milk ($204{\pm}46d$). The cows were randomly assigned within blocks to one of three treatments: 0 (control), 120, or 240 g/d of SCFP mixed with 240, 120, or 0 g of corn meal, respectively. The experiment was carried out during the summer season of 2014, starting from 14 July 2014 and lasting for 9 weeks with the first week as adaption period. During the experimental period, average daily temperature-humidity index (measured at 08:00, 14:00, and 20:00) was above 68, indicating that cows were exposed to heat stress throughout the study. Rectal temperatures tended to decrease linearly (p = 0.07) for cows supplemented with SCFP compared to the control cows at 14:30, but were not different at 06:30 (p>0.10). Dry matter intake was not affected by SCFP supplementation (p>0.10). Milk yield increased linearly (p<0.05) with increasing levels of SCFP. Feed efficiency (milk yield/ dry matter intake) was highest (p<0.05) for cows fed 240 g/d SCFP. Cows supplemented with SCFP gained (p<0.01) body weight, while cows in the control lost body weight. Net energy balance also increased linearly (p<0.01) with increasing levels of SCFP. Concentrations of milk urea nitrogen (p<0.01) decreased linearly with increasing levels of SCFP, while no difference (p>0.10) was observed among the treatments in conversion of dietary crude protein to milk protein yield. In summary, supplementation of SCFP alleviated the negative effect of heat stress in lactating Holstein dairy cows and allowed cows to maintain higher milk production, feed efficiency and net energy balance. Effects of SCFP were dose-dependent and greater effects were observed from higher doses.

• Korean Journal of Veterinary Research
• /
• v.43 no.4
• /
• pp.555-561
• /
• 2003

Incidence of estrum or abortions in pregnant cows may be affected by large follicles developed together with corpus luteum in pair ovaries of pregnant cows. But the follicles of pregnant phase were not assessed about histological findings. Determination of the healthy and atretic follicles by presence of proliferative cells or apoptotic cells and histological compositions of follicles would be used as important data on measurements of ovarian functions. This study was focussed mainly to investigate macroscopical, histological and immunohistochemical findings of ovarian follicles of pregnant Korean native cows and dairy cows (Holstein). In immunohistochemical methods, assessments of proliferative cells using PCNA antibody and apoptotic cells using TUNEL methods were performed. The follicles were observed on all 24 pregnant cows (17 Korean native cows and 7 Holstein cows). Follicles of greater than 10 mm in daimeter were developed in 37.5% (9/24 heads) of these pregnant cows. largest follicles from in these cows were $16.0{\times}15.0mm$ in diameter in a Korean native cow(l20 days of gestation), $13.4{\times}10.1mm$ in a Korean native cow(50 days of gestation), $12.9{\times}11.5mm$ in a Holstein cow (120 days of gestation). 40.5% among all follicles having diameter of greater than 1.0 mm in pregnant cows were assessed as atretic follicles and in addition, healthy follicles also showed less in number and smaller in size and thinner in wall layer compared with those of cyclic phase ovaries. In immunohistochemical findings, also proliferative positive cells and apoptotic positive cells on the granulosa cell layers in the healthy follicles of pregnant cows appeared less than on those of cyclic follicles. So these follicles were assessed as weakly active follicles. In large follicles, above positive cells were not nearly appeared but granulosa cell debris were more appeared among the granulosa cells. So these large follicles were assessed as inactvie or atretic follicles. The above findings suggest that small follicles of pregnant phase were weakly active or atretic and large follicles were inactive or atretic.

• Asian-Australasian Journal of Animal Sciences
• /
• v.19 no.10
• /
• pp.1455-1463
• /
• 2006

Effects of different levels of molasses in a concentrate supplement on milk yield of cows grazing Setaria grass pastures were investigated. Thirty Friesian cows, 6-7 years old with mean pre-experimental body weight of $428{\pm}6.5$ kg, in early stage of lactation were randomly allotted to five dietary treatments in a completely randomized design experiment that lasted for 126 days. Experimental dietary treatments were forage alone and forage/concentrate mixtures with molasses included at 0, 5, 10 or 15% levels designated as $T_1$, $T_2$, $T_3$, $T_4$ and $T_5$, respectively. The parameters studied were voluntary dry matter (DM) intake, average daily live weight change (LWC), milk yield, body condition score (BCS) and apparent nutrient digestibility coefficients. The DM and energy contents of Setaria grass were low compared to the concentrate diets. Fibre fractions-NDF, ADF, ADL, hemicellulose and cellulose; and gross energy were higher in concentrate mixtures than in the forage. Total DM intake (forage+concentrate mixtures) was significantly higher (p<0.001) in cows on the concentrate mixtures. LWC was not significantly different (p>0.05) between the cows. Average milk yields were significantly different (p<0.05) between cows. Fat corrected milk (FCM) was similar among cows in the treatments. BCS was better (p<0.001) in cows on concentrate mixtures. Digestibilities of DM, CP, NDF, ADF, ADL, OM, and energy were significantly higher (p<0.001) in cows on $T_2$, $T_3$, $T_4$ and $T_5$ than in those on $T_1$. There were no significant differences in the digestibility of DM, CP, NDF, ADF and ADL (p>0.001) in cows on concentrate mixtures. This study therefore demonstrated that lactating dairy cows in Fiji need a level of readily fermented energy source such as molasses in their diets; however, a level above 10% is not nutritionally suitable for lactating dairy cows. Based on data on production parameters-milk yield, fat corrected milk, body condition score and apparent nutrient digestibility coefficients-molasses levels that range between 5-10% are recommended, however, 10% is the best and therefore recommended for inclusion in the concentrate mixture of lactating dairy cows on a basal diet of Setaria sphacelata in Fiji.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.3
• /
• pp.404-413
• /
• 2008

Major objectives were to evaluate effects of three schemes of bST-supplementation of Holstein cows (142.8 mg/14 d, POSILAC) during the prepartum and/or postpartum periods through 63 d (${\pm}3d$) of lactation. Measures evaluated the potential of treatments to improve body weight (BW) and body condition score (BCS), provoke changes in plasma concentrations of somatotropin (ST) and IGF-I, and improve milk yield, milk composition (percentages of protein and fat, and somatic cell counts), and several calving variables. Multiparous Holstein cows were randomly assigned to a $2{\times}2$ factorial arrangement of treatments (TRT) to give four groups (I = no bST, n = 26; II = bST postpartum, n = 25; III = bST prepartum, n = 27; IV = bST prepartum and postpartum, n = 25). During the prepartum period, cows in groups I and II were not supplemented but those in groups III and IV were supplemented every 2-wk beginning 21 d before expected calving date through calving. During the first 63 DIM only cows in groups II and IV were supplemented with bST. From 64 DIM through the end of lactation cows in all groups were supplemented with the full lactation dose of bST (500 mg/14 d). The BW and BCS were recorded weekly throughout the prepartum and postpartum periods and every 2-wk beyond 70 DIM. Blood samples were collected 3-times a week for analyses of ST and IGF-I. Milk yields were recorded daily though 150 DIM. Prepartum supplementation of bST did not affect BW or BCS, but mean concentrations of ST were increased 12.2% and were 15.5% greater at calving. Overall, mean concentration of IGF-I was not affected by treatment but concentrations were greater at 1 and 2 wk before calving in bST-supplemented cows. During the first 63 DIM the BW and BCS were not affected by treatment. Significant effects of bST-supplementation were detected on concentrations of ST, IGF-I and on milk yield compared to non-supplemented cows in group I. Postpartum concentrations of ST were greater in bST-supplemented cows (TRT II and IV; +41.9 and 54.6%). However, concentrations of IGF-I were greater only in cows in group IV (+25.9%) during the postpartum period. Overall, the three bST-supplemented groups had greater actual milk yield than the control group (I) during the first 63 and 150 DIM. The actual milk yields during 63 and 150 DIM were 6.5 and 4.6 kg/d greater for cows in group IV than cows in group I and the 305-d ME milk yield also was 15.6% greater. No adverse effects of TRT were observed on calf birth weight, colostrum immunoglobulins, ease of calving or other measures evaluated.

• Korean Journal of Veterinary Research
• /
• v.15 no.2
• /
• pp.161-176
• /
• 1975

In order to evaluate the epizootiological influence of high-land on cattle feeding, routine hematological observation, with additional examinations of feces and general clinical condition, was conducted comparing the high-land cows resident at altitude of 800 to 1,200 meters in Daegwanryeong to the low-land cows resident at altitudes of less than 100 meters in Samcheok and Gangneung. The high-land cows were divided into 3 groups such as group A, B and C, consisting of 15, 16 and 20 adult cows respectively, and among the 3 groups only group C was exposed to abrupt high-land cold before observation. The low-land cows, that is group D, were consisted of 25 adult cows. The results obtained in the survey were summarized as follows: 1. Number of erythrocytes, concentration of hemoglobin and packed cell volume were remarkably higher (p<0.01) in all of 3 groups (group A, B and C) of high-land cows than trios of low-land cows (group D). These higher values in high-land cows were ascribable to the better feeding and hygienic management, and lesser infestation of small-type Piroplasma (Theileria) and internal parasites in contrast with the low-land cows. 2. Remarkably higher value of mean corpuscular volume (p<0.01) and a tendency to lower value of mean corpuscular hemoglobin concentration were noted in group D in contrast with group A, B and C. The reason was attributed to the higher infestation (p<0.01) of smalltype piroplasma in low-land cows in contrast with high-land cows. 3. It was noticiable that even though lesser infestation of small type Piroplama in the highland cows was shown, a natural case of clinical small-type-piroplasmosis was found in high-land group B as well as a case in the low-land group D. The blood Pattern showed characteristic macrocytic-hypochromic-anemia with increased reticulocytes in both two cases (Table 7). 4. In the observation of leukocytic series, marked increase of eosinophils in group D was seen in accordance with heavy infestation of gastrointestinal nematodes, and increased neutrophils with higher appearance of nonlobulated form and eosinopenia in group C only was observed suggesting the leukocytic response to the cold exposure in high-land. 5. Mean values of thrombocytes in group A,B,C and D were 48.4, 40.7, 42.7 and $32.3{\times}10^4/mm^3$ respcetively, and no statistically significant differences were observed.

• Journal of Animal Reproduction and Biotechnology
• /
• v.35 no.1
• /
• pp.50-57
• /
• 2020

The objective of this research work was to know ovarian dynamics and pregnancy rate of cyclic Murrah buffalo cows with induced estrus by administration of prostaglandin F2α (PGF2α) and timed artificial insemination (TAI) with frozen thawed semen. A total of 31 female buffaloes were selected for the study. The buffalos having matured CL observed by ultrasonography were given one intra muscular injection of cloprostenol 500 ㎍ and TAI was performed using frozen thawed semen of Indian Murrah buffalo bull. Results showed that 90.32% (significantly, at p < 0.05) cows explore the sign of heat after injection of PG and 67.85% (significantly, at p < 0.05) cows were become pregnant out of 28 inseminated (TAI) cows. In the 28 inseminated (TAI) cows, average number of smaller and larger size of follicles were non-significantly (p > 0.05) higher at day 3 post PG injection, but the medium size of follicles was nonsignificantly (p > 0.05) higher at PG injection. At day 3 post PG injection the diameter of follicles was significantly (p < 0.05) higher, but the diameter of CL was significantly (p < 0.01) lower compared at PG injection. At PG injection the diameter of largest follicle was non-significantly differences (p > 0.05) in between pregnant and non-pregnant cows. But at day 3 post PG injection it was significantly (p < 0.01) higher in pregnant cows compared to non-pregnant cows. The number of small, medium, and large follicles at PG injection and at day 3 post PG injection were non-significantly (p > 0.05) difference in between pregnant and non-pregnant buffalo cows. Finally, it is concluded that the CL was effectively regresses and induced the sign of heat in buffalo cows and after AI the cows were become pregnant with significant rate. The study will help to the veterinarian and researcher to know the efficacy of PG injection and AI for reproductive efficiency in buffalo cows.

• Asian-Australasian Journal of Animal Sciences
• /
• v.11 no.3
• /
• pp.265-267
• /
• 1998

Cattle in Alaska seemed to be tolerant to low blood selenium (Se) although arctic winter energy demands might exacerbate oxidative damage to tissues lacking protection by Se. The thermogenic properities of brown adipose tissue (BAT) and the long cold adaptation period for Alaskan cattle indicates that they might develop BAT. Eighteen mature beef cows with whole blood Se of< 19 ppb were used to examine possible relation-ships among Se deficiency, tissue lesions, and observable BAT. All cows were wintered on a diet low in Se and nine cows were given supplemental Se provided by intraruminal bolus. Blood Se was elevated (p < .05) from November through February by supplemental Se, but body weight and back fat thickness were unaffected (p < .10). Tissues were taken from two Se deficient cows, two cows supplemented with Se, and two cows given large doses of Se (300 mg by injection) four weeks before slaughter. Histopathological examination of 187 samples of fat (7 to 55 from each cow) showed no observable amount of BAT. Examination of other tissues showed no lesions attributable to Se deficiency.

• Journal of Veterinary Clinics
• /
• v.5 no.2
• /
• pp.95-99
• /
• 1988

An observational study on the recurrences of left abomasal displacement after surgical correction was conducted in 178 Holstein cows. The correction methods were right flank omentopexy (A), right ventral paramedian abomasopexy (B) and left flank abomasopexy (C). Four(5.4) out of 74 cows by method A, 2(2.0) out of 101 cows by method B and 1(33.3%) out of 3 cows by method C had the recurrence of the disease after the first operation. As for the method A, 3 cows and a cow recurred 13-18 months and 27 months after the first operation, respectively. As for the method B, recurrence of the disease in two cows occurred 14 and 19 days after surgery, respectively. In a cow which had been indicated by method C, the recurrence was recurred 14 months after the operation.

• Korean Journal of Veterinary Research
• /
• v.35 no.3
• /
• pp.631-633
• /
• 1995

70 cows with abomasal torsion and 71 cows with right-side displacement of the abomasum were examined to determine whether the preoperative blood base-excess concentration could be used both as a prognostic indicator for postoperative recovery in cows with abomasal torsion and as an aid in differentiating between abomasal torsion and right-side displacement of the abomasum. The survival rate of cows with abomasal torsion decreased as the base-excess concentration decreased(P=0.08). There was a significant difference(P<0.025) among base-excess ranges between abomasal torsion and right-side displacement of the abomasum. Most cows with base-excess concentrations ${\geq}15.0mEq/L$ and ${\leq}-0.5mEq/L$ had abomasal torsion, rather than right-side displacement of the abomasum.

• Asian-Australasian Journal of Animal Sciences
• /
• v.27 no.8
• /
• pp.1104-1113
• /
• 2014

The effects of feeding pelleted citrus pulp (PCP) as a natural antioxidant source on the performance and milk quality of dairy cows fed highly polyunsaturated fatty acid (FA) diets were evaluated. Four lactating Holstein cows were assigned to a $4{\times}4$ Latinsquare. Treatments, on a dry matter (DM) basis, were i) control diet; ii) 3% soybean oil; iii) 3% soybean oil and 9% PCP and; iv) 3% soybean oil and 18% PCP. When cows fed on citrus pulp, the DM intake tended to decrease. The total tract apparent digestibility of DM and ether extract decreased when cows fed on the control diet compared to other diets. Cows fed PCP had higher polyphenols and flavonoids content and higher total ferric reducing antioxidant power (FRAP) in milk compared to those fed no pelleted citrus pulp. Cows fed 18% PCP showed higher monounsaturated FA and lower saturated FA in milk fat compared with cows fed the other diets. The lowest n-6 FA proportion was in milk fat from cows fed control. The present study suggests that pelleted citrus pulp added to 9% to 18% DM increases total polyphenols and flavonoids concentration, and the FRAP in milk.