Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Monitoring the Sonographic Ovarian Dynamics and Pregnancy Rate in Cyclic Murrah Buffalo Cows Synchronized with Prostaglandin F2α

  • Harun-or-Rashid, Mohammad (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Phulia, SK (Division of Animal Physiology and Reproduction, Central Institute for Research on Buffalo (CIRB)) ;
  • Hasan, Mir Md. Iqbal (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Musharraf, Mohammad (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Bhuiyan, Uddin (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Juyena, Nasrin Sultana (Department of Surgery and Obstetrics, Bangladesh Agricultural University) ;
  • Sharma, Rakesh Kumar (Division of Animal Physiology and Reproduction, Central Institute for Research on Buffalo (CIRB))
  • Received : 2019.10.20
  • Accepted : 2020.03.10
  • Published : 2020.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of this research work was to know ovarian dynamics and pregnancy rate of cyclic Murrah buffalo cows with induced estrus by administration of prostaglandin F2α (PGF2α) and timed artificial insemination (TAI) with frozen thawed semen. A total of 31 female buffaloes were selected for the study. The buffalos having matured CL observed by ultrasonography were given one intra muscular injection of cloprostenol 500 ㎍ and TAI was performed using frozen thawed semen of Indian Murrah buffalo bull. Results showed that 90.32% (significantly, at p < 0.05) cows explore the sign of heat after injection of PG and 67.85% (significantly, at p < 0.05) cows were become pregnant out of 28 inseminated (TAI) cows. In the 28 inseminated (TAI) cows, average number of smaller and larger size of follicles were non-significantly (p > 0.05) higher at day 3 post PG injection, but the medium size of follicles was nonsignificantly (p > 0.05) higher at PG injection. At day 3 post PG injection the diameter of follicles was significantly (p < 0.05) higher, but the diameter of CL was significantly (p < 0.01) lower compared at PG injection. At PG injection the diameter of largest follicle was non-significantly differences (p > 0.05) in between pregnant and non-pregnant cows. But at day 3 post PG injection it was significantly (p < 0.01) higher in pregnant cows compared to non-pregnant cows. The number of small, medium, and large follicles at PG injection and at day 3 post PG injection were non-significantly (p > 0.05) difference in between pregnant and non-pregnant buffalo cows. Finally, it is concluded that the CL was effectively regresses and induced the sign of heat in buffalo cows and after AI the cows were become pregnant with significant rate. The study will help to the veterinarian and researcher to know the efficacy of PG injection and AI for reproductive efficiency in buffalo cows.

Keywords

References

  1. Amaya-Montoya C, Matsui M, Kawashima C, Hayashi KG, Matsuda G, Kaneko E, Kida K, Miyamoto A, Miyake Y. 2007. Induction of ovulation with GnRH and PGF(2 alpha) at two different stages during the early postpartum period in dairy cows: ovarian response and changes in hormone concentrations. J. Reprod. Dev. 53:867-875. https://doi.org/10.1262/jrd.18163
  2. Awasthi MK, Tiwari RP, Pangaonkar GR. 1998. Induction of estrus and fertility with low dose of prostaglandin F2 alpha in subestrus buffaloes. Indian J. Anim. Sci. 68:1049-1050.
  3. Baruselli PS, Berber RA, Madureira EH, Carvalho NT. 2003. Half dose of prostaglandin F2a is effective to induce luteolysis in the synchronization of ovulation protocol for fixed-time artificial insemination in buffalo (Bubalus bubalis). Braz. J. Vet. Res. Anim. Sci. 40:397-402.
  4. Baruselli PS, Madureira EH, Visintin JA, Barnabe VH, Barnabe RC, Amaral R. 1999. Inseminacao artificial em tempo fixo com sincronizacao da ovulacao em bubalinos [Timed artificial insemination with synchronization of ovulation in buffaloes]. Rev. Bras. Reprod. Anim. 23:360-363.
  5. Baruselli PS, Soares JG, Gimenes LU, Monteiro BM, Olazarri MJ, Carvalho NAT. 2013. Control of buffalo follicular dynamics for artificial insemination, superovulation and in vitro embryo production. Buffalo Bull. 32:160-176.
  6. Campanile G, Baruselli PS, Neglia G, Vecchio D, Gasparrini B, Gimenes LU, Zicarelli L, D'Occhio MJ. 2010. Ovarian function in the buffalo and implications for embryo development and assisted reproduction. Anim. Reprod. Sci. 121:1-11.
  7. Chohan KR. 1998. Estrus synchronization with lower dose of PGF2 alpha and subsequent fertility in subestrous buffalo. Theriogenology 50:1101-1108. https://doi.org/10.1016/S0093-691X(98)00211-8
  8. Cooper MJ and Rowson LEA. 1975. Control of the oestrous cycle in Friesian heifers with ICI 80,996. Ann. Biol. Anim. Biochim. Biophys. 15:427-436. https://doi.org/10.1051/rnd:19750232
  9. De Rensis F and Lopez-Gatius F. 2007. Protocols for synchronizing estrus and ovulation in buffalo (Bubalus bubalis): a review. Theriogenology 67:209-216. https://doi.org/10.1016/j.theriogenology.2006.09.039
  10. Dhaliwal GS, Sharma RD, Singh G. 1988. Efficacy of prostaglandin F2-alpha administration for inducing estrus in buffalo. Theriogenology 29:1401-1406. https://doi.org/10.1016/0093-691X(88)90021-0
  11. Drost M and Elsden RP. 1985. Blastocyst development in the water buffalo (Bubalus bubalis). Theriogenology 23:191. https://doi.org/10.1016/0093-691X(85)90097-4
  12. Edmondson AJ, Fissore RA, Pashen RL, Bondurant RH. 1986. The use of ultrasonography for the study of the bovine reproductive tract. I. Normal and pathological ovarian structures. Anim. Reprod. Sci. 12:157-165. https://doi.org/10.1016/0378-4320(86)90036-9
  13. El-Din Zain A, Abdel-Razek AKh, Anwar MM. 2001. Effect of combined using of GnRH and $PGF2{\alpha}$ on oestrus synchronization and pregnancy rate in buffalo-cows. Assiut Vet. Med. J. 45:303-316.
  14. Farin PW, Youngquist RS, Parfet JR, Garverick HA. 1990. Diagnosis of luteal and follicular ovarian cysts in dairy cows by sector scan ultrasonography. Theriogenology 34:633-642. https://doi.org/10.1016/0093-691X(90)90019-P
  15. Galina CS and Orihuela A. 2007. The detection of estrus in cattle raised under tropical conditions: what we know and what we need to know. Horm. Behav. 52:32-38. https://doi.org/10.1016/j.yhbeh.2007.03.025
  16. Ginther OJ, Knopf L, Kastelic JP. 1989. Ovarian follicular dynamics in heifers during early pregnancy. Biol. Reprod. 41:247-254. https://doi.org/10.1095/biolreprod41.2.247
  17. Kahn W and Leidl W. 1989. Ultrasonic characteristics of pathological conditions of the bovine uterus and ovaries. In: Taverne MAM, Willemse AH (Eds.), Diagnostic Ultrasound and Animal Reproduction. Current Topics in Veterinary Medicine and Animal Science, v. 51., Kluwer Academic Publishers, Dordrecht, pp. 53-65.
  18. Kamonpatana M, Pansin C, Jetna T, Sophon S, Sravasti S, Srisakwattana K. 1987. Factors causing low conception rates when PGF2 is used for oestrus synchronization in swamp buffaloes. Buffalo J. 1:127-143.
  19. Lamb GC, Cartmil JA, Stevenson JS, 2004. Effectiveness of Select Synch (gonadotropin-releasing hormone and prostaglandin $F2{\alpha}$) for synchronizing estrus in replacement beef heifers. Prof. Anim. Sci. 20:27-33. https://doi.org/10.15232/S1080-7446(15)31268-7
  20. Lamb GC, Nix DW, Stevenson JS, Corah LR. 2000. Prolonging the MGA-prostaglandin F2 alpha interval from 17 to 19 days in an estrus synchronization system for heifers. Theriogenology 53:691-698. https://doi.org/10.1016/S0093-691X(99)00267-8
  21. Madan ML. 1988. Status of reproduction in female buffalo. Buffalo Production and Health: A Compendium of Latest Research Information Based on Indian Studies. ICAR Publication, New Delhi, India, pp. 89-100.
  22. Odde KG. 1990. A review of synchronization of estrus in postpartum cattle. J. Anim. Sci. 68:817-830. https://doi.org/10.2527/1990.683817x
  23. Plata NI, Spitzer JC, Thompson CE, Henricks DM, Reid MP, Newby TJ. 1990. Synchronization of estrus after treatment with Luprostiol in beef cows and in beef and dairy heifers. Theriogenology 33:943-952. https://doi.org/10.1016/0093-691X(90)90057-Z
  24. Prakash BS. 2002. Influence of environment on animal reproduction. National Workshop on Animal Climate Interaction. Izatnagar, India, pp. 33-47.
  25. Presicce GA, Bella A, Terzano GM, De Santis G, Senatore EM. 2005. Postpartum ovarian follicular dynamics in primiparous and pluriparous Mediterranean Italian buffaloes (Bubalus bubalis). Theriogenology 63:1430-1439. https://doi.org/10.1016/j.theriogenology.2004.07.003
  26. Pursley JR, Wiltbank MC, Stevenson JS, Ottobre JS, Garverick HA, Anderson LL. 1997. Pregnancy rates per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J. Dairy Sci. 80:295-300. https://doi.org/10.3168/jds.S0022-0302(97)75937-X
  27. Randel RD, Del Vecchio RP, Neuendorff DA, Peterson LA. 1988. Effect of alfaprostol on postpartum reproductive efficiency in Brahman cows and heifers. Theriogenology 29:657-670. https://doi.org/10.1016/S0093-691X(88)80012-8
  28. Reeves JJ, Rantanen NW, Hauser M. 1984. Transrectal real-time ultrasound scanning of the cow reproductive tract. Theriogenology 21:485-494. https://doi.org/10.1016/0093-691X(84)90410-2
  29. Sahasrabudhe SA and Pandit RK. 1999. Comparative efficiency of PGF2 alpha administration through different routes in subestrus buffaloes during summer season. Indian Vet. J. 76:128-130.
  30. Savio JD, Keenan L, Boland MP, Roche JF. 1988. Pattern of growth of dominant follicles during the oestrous cycle of heifers. J. Reprod. Fertil. 83:663-671. https://doi.org/10.1530/jrf.0.0830663
  31. Settergren I. 1987. Female reproduction. State of Knowledge. Proc. International on Milk Buffalo Reproduction. Islamabad, Pakistan, pp. 21-48.
  32. Singh G, Chandolia RK, Dutt R, Saini A, Dalal J, Malik RK. 2017. Comparative study of follicular turn over in cyclic and noncyclic Murrah buffaloes through 2D trans-rectal ultrasonography. Int. J. Curr. Microbiol. App. Sci. 6:3590-3602. https://doi.org/10.20546/ijcmas.2017.611.421
  33. Situmorang P and Siregar AR. 1998. Effects of hormone hCG following injection of Estrumate of reproductive performances of swamp buffalo (Bubalus bubalis). J. Ilmu Ternak Vet. 2:213-217.
  34. Stotts J, Stumpf T, Day M, Wolfe M, Wolfe P, Kittok R, Nielsen M, Deutscher G, Kinder J. 1987. Luteinizing hormone and progesterone concentrations in serum of heifers administered a short half-life prostaglandin (PGF(2alpha)) or long halflife prostaglandin analogue (fenprostalene) on days six or eleven of the estrous cycle. Theriogenology 28:523-529. https://doi.org/10.1016/0093-691X(87)90257-3
  35. Twagiramungu H, Guilbault LA, Proulx JG, Dufour JJ. 1994. Influence of corpus luteum and induced ovulation on ovarian follicular dynamics in postpartum cyclic cows treated with buserelin and cloprostenol. J. Anim. Sci. 72:1796-1805. https://doi.org/10.2527/1994.7271796x
  36. Ty LV, Nguyen BX, Son HN, Driancourt MA. 1994. Superovulation and ovarian follicular population of juvenile buffaloes and calves. Anim. Reprod. Sci. 35:191-199. https://doi.org/10.1016/0378-4320(94)90035-3
  37. Yilmaz O, Yazici E, Kahraman A, Ozenc E, Ucar M. 2014. The relationship between ovarian follicle population and follicle size during different stages of estrous cycle in Anatolian Water buffaloes (Bubalus bubalis). Rev. Med. Vet. (Toulouse) 165:111-115.