• 한국초지조사료학회지
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• 제12권1호
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• pp.71-76
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• 1992

Starch gel electrophoresis was used to examine the banding pattern of Esterase isozyme in the leaf, root-nodule and seedling of four wild legume species, Trifolim repense, Glycine soja, Phaseolus nipponensis and Vigna uexillata. The number of band, enzyme activity and migrating rate of esterase isozyme varies depending on the species and tissues of legume plants. The isozyme banding pattern in the cotyledon and radicle of T. repense showed same pattern, however, the number of band were varible among the cotyledon of G. soja, P. nipponensis and V. vexzllata, respectively. Est-1 in the leaf of G. soja, V, vexillata. root-nodule of G. soja and seedling of V. vexillata expreesed the highest enzyme activity. The Est-1 showed the rapidest migrating rate among the isozymes.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.305-305
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• 2017

Seoritae with green cotyledons refers to Korean native black beans harvested when the frost falls because the maturity is late. It is one of the beans preferred by consumers because of its softness and high sugar content. Because of late maturity, if the agricultural work is delayed by weather or agricultural schedule in green cotyledon black soybean (Seoritae), it affects seriously harvest and yield of soybeans. The aim of this study was to investigate the marginal seeding period on June 30, July 10 and July 20 in Cheongju and Jecheon area in Korea to produce stable soybean yield. The yields of green cotyledon black soybean as seeding date in Cheongju area are as follows: Seolitae (Yeoncheon), Seoltae (Goesan) and Seoritae (Gogseong), which increased by 23%, 56%, 23% and 40%, respectively, compare to July 10th to June 30th. As soybean sowing is delayed, the quality of soybean seeds has decreased due to the increase of immature seeds and fungal damaged seeds. The contents of anthocyanin in Cheongju area a functional substance of soybeans, was high on July 20 for Heukcheong, on June 30 for Seolitae (Yeoncheon), on July 10 for Seoritae (Goesan). The yields of Heukcheong and Seolitae (Yeoncheon) in Jecheon area were increased by 5% and 17%, respectively, compare to July 10th to June 30th, while Seolite (Goesan) and Seolite (Gogseong) were high in yields on June 30th. Similarly in Cheongju area, as the sowing period is delayed, the number of immature and mold damaged seeds in Jecheon area increased. The contents of anthocyanin in Jecheon area was high on July 10 for Heukcheong, on June 30 for Seolitae (Yeoncheon) and Seolitae (Goesan). From the above results, Sowing marginal date of green cotyledon black soybeans (Seolitae) in Cheongju area increased 30% in sowing on July 10 and increased 2% in sowing on July 20 compared to June 30. And Sowing marginal date of green cotyledon black soybeans (Seolitae) in Jecheon area increased 2% in July 10 compared to June 30, and the yield decreased rapidly on July 20. We have identified the seeding time limit of green cotyledon black bean in Chungbuk province. It will be possible to provide a variety of crop selection after double cropping of farmers. And by knowing the yield and seed quality of soybean according to sowing date, farmers will observe appropriate sowing period of soybeans for high quality. From the viewpoint of consumers, functional substances of Seoritae will meet the desire for health.

• Journal of Plant Biotechnology
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• 제33권4호
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• pp.263-270
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• 2006

Pongamia pinnata Pierre is a tree legume, having potential in production of raw material for biodiesel. A protocol for in wk propagation of this plant was standardized using seedling explants. Growth regulators (GR) including gibberellic acid $(GA_3),\;N^6-benzylaminopurine(BA)$, thidiazuron (TDZ), and Adenine sulphate (Ads) were tested for optimum germination of seeds. Removal of seed coat prior to germination, controlled fungal growth partially but enhanced bacterial growth. Antibiotic cefotaxime was ineffective in controlling bacterial contamination. Seedling derived nodal explants and cotyledon nodes with attached cotyledons were excised and cultured for induction of shoots. Optimum sprouting and multiplication of shoot buds were obtained in MS medium supplemented with $8.88{\mu}M$ BA. These buds differentiated and rooted on medium devoid of GR. Optimum growth of Pongamia seedling was obtained in cotton plugged culture vessels. Reculturing of the cotyledon node explants produced more shoots from the same site. This process of removing shoots and reculturing of cotyledon node was followed for eight passages yielding 4 to 8 shoots in each cycle. The shoots (75%) rooted on half strength MS basal medium supplemented with 0.22% charcoal. All plants survived on transfer to soil. This is the first report on in vitro regeneration of Pongamia pinnata. This report demonstrates the possibility of coupling more than one parameter in single experiment to hasten the process of standardization. The process of cycling the nodal explant repeatedly for production of large number of shoots from single meristem may find application in genetic transformation experiments wherein meristems are used for transformation.

• 생물환경조절학회지
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• 제14권1호
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• pp.56-62
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• 2005

시설참외 32공 플러그트레이 육묘시 접목방법이 따른 묘 소질과 정식 후 생육과 수량에 미치는 영향을 구명코자하였다. 접목방법은 관행의 호접과 편엽합접 및 단근편엽합접을 실시하였다. 접목 후 15일 후 묘소질은 호접이 엽수와 엽면적에서 높았으나 이후 점차 감소되어 접목 후 20일에는 초장, 경경, 엽록소함량이 비슷하였고, 접목 후 25일에는 단근편엽합접에서 초장, 엽수, 엽면적 등 지상부 생육이 가장 높았으며 지하부도 근건중이 높아 T/R율이 가장 낮은 경향을 보여 정식 전 묘 소질이 가장 좋게 나타났다. 본포 생육은 초장, 엽수, 엽면적 그리고 건물중에서 정식 후 30일 단근편엽합접이 가장 높았으며 첫 개화소요일수도 38.4일로 가장 빨랐다. 그러나 과실의 품질은 과중, 당도. 경도, 색도에서 접목방법별 차이가 없었다 따라서 접목방법의 차이가묘 소질과 정식 후 초기생육과 수량에 영향을 미치는 것으로 나타나 접목방법의 전환이 요구되는 것으로 나타났다.

• Journal of Ginseng Research
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• 제31권1호
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• pp.14-22
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• 2007

As the zygotic embryo of North American ginseng (Panax quinquefolius L.) matured during stratification over 203 days it grew from 0.75 to 5.2 mm. Embryo excision and culturing on media containing different concentrations of two growth regulators, gibberellic acid ($GA_3$, 1 to 10 ${\mu}M$) and benzyladenine (BA, 1 to 5 ${\mu}M$), during stratification, showed that shoot and root number and the shoot, root and cotyledon length increased with increased stratification time. Gibberellic acid was the more effective growth regulator for increasing shoot and root number and shoot, root and cotyledon lengths. Immature embryos (stratified for up to 63 days) needed growth regulators for further development. Cultures on $GA_3$ at the last culture date (stratified for 203 days) when embryos were mature, produced multiple shoots but there was no effect of $GA_3$ concentration. Benzyladenine inhibited shoot and root growth regardless of embryo stratification. Growth regulators had little effect on cotyledon length of mature embryos. Embryos cultured on $GA_3$ combined with BA were green on all culture dates whereas greening in the control and BA treatments increased with culture date. The BA treatments induced 100% swelling of the embryos on the final culture date while in the control and $GA_3$ treatments there was no swelling. There was little or no curling in the control and BA treatments and a linear decrease in curling with culture date in the $GA_3$ and $GA_3$ + BA treatments.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.176-176
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• 2017

Hydropriming had positive effects on the time for germination to reach 50%, the germination index, the time to final germination percentage, and the number of uniform seedlings with enlarged cotyledons in in vitro germination of small watermelon. In addition, the highest shoot initiation was obtained from hydroprimed cotyledonary nodes ($95{\pm}6%$), followed by non-primed cotyledonary nodes ($78{\pm}6%$), hydroprimed cotyledons ($72{\pm}4%$), and non-primed cotyledons ($48{\pm}4%$). Meanwhile, no shoots were initiated from hypocotyls. The total number of shoots that initiated from cotyledonary nodes and cotyledon explants was insignificant, indicating that both cotyledons and cotyledonary node were good sources for the in vitro culture. Choosing explant sources that favor tetraploidy should be the key for producing higher polyploidy plants; a total of 10.5% of tetraploid regenerants were entirely identified from cotyledon explants. Cotyledons with highly differentiated cells might show higher variations than cotyledonary nodes with more preexisting meristematic cells. Cells of cotyledon tissue might undergo changes in ploidy level during differentiation of the culture, or it might be that some of the variations were already present in the tissues of the donor plant. Morphological changes in fruit length of tetraploid regenerants are genotype-dependent.

• 한국산림과학회지
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• 제40권1호
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• pp.70-74
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• 1978

잣나무묘목(苗木)의 자엽수(子葉數)에 따른 성장(成長) 및 침엽형질(針葉形質)과의 상관(相關)을 구명(究明)하고져 자엽수별(子葉數別)로 등급화(等級化)한 묘목(苗木)의 초생엽(初生葉), 본엽(本葉), 그리고 묘목성장(苗木成長) 등(等)의 형질(形質)을 조사(調査)한 결과(結果)를 다음과 같이 요약(要約)한다. 1. 초생엽(初生葉)의 엽수(葉數)와 자엽수간(子葉數間)에 1%의 유의성(有意性)이 있었고 초생엽장(初生葉長), 폭(幅), 기공선수(氣孔線數)에 관(關)하여는 유의성(有意性)을 인정(認定)할 수 없었다. 2. 초생엽(初生葉)의 부수지구(副樹脂溝)는 6월말경(月末頃)까지 거의 발달(發達)되지 않았고 소수(少數)는 7월(月) 이후(以後)부터 1~2개(個) 발달(發達)하기 시작하였으며 자엽수(子葉數)에 따른 부수지구수(副樹脂溝數)의 차이(差異)는 없었다. 3. 본(本) 엽수(葉數)와 자엽수간(子葉數間)에는 5%의 유의성(有意性)을 인정(認定)할 수 있었으나 엽장(葉長), 엽폭(葉幅), 기공선수(氣孔線數)는 자엽수(子葉數)와 관련(關聯)이 없었다. 4. 묘고(苗高), 근원직경(根元直徑)은 자엽수(子葉數)와 5%의 유의성(有意性)이 있었다. 5. 묘목성장(苗木成長)을 보연 자엽수(子葉數) 12매이상(枚以上)인 묘목(苗木)은 성장(成長)이 매우 양호(良好)하였다. 그래서 그 이하(以下)의 것은 간인(間引)의 대상이 될 것으로 생각되었다.

• Plant Resources
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• 제2권1호
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• pp.16-21
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• 1999

An efficient regeneration system was established by using in vitro plantlets of germinated seedlings from different cultivars of lettuce (Lactuca sativa cv. Chongchima, Chongchuckmyun, Jeokchima, Jeokchuckmyun). Shoot formation were observed from all cultivars on MS medium supplemented with 0.1 mg/L NAA and 0.5 mg/L BA. In all cultivars, when cotyledon was cultured, the number of shoot per explant was more greater than that hypocotyl and leaf disc were cultured. Shoot formation rate (91.7%) was high in a cotyledon culture of cultivar, Chongchukmyun. The growth of multiple shoots derived from the cultivar, Chongchukmyun, was most effective on medium containing 0.5 mg/L BA and 1.0 mg/L GA$_3$. When shoots were transferred on MS medium without plant growth regulators, roots were effectively differentiated. Rooted plantlets were acclimated on pots for further propagation.

• 원예과학기술지
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• 제35권2호
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• pp.265-275
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• 2017

Selection of double-flowered plants at the seedling stage is one of the main purposes of stock breeding programs. Eight stock cultivars of Matthiola incana L. named 'Nobel', 'Cinderella', 'Pacific', 'Avalanche', 'Midblue', 'Lavender', 'Goddess' and 'Esfahan', with different percentage of double-flowered plants were used for examining the relationship with three morphological types of cotyledons. The results of a chi-square test indicated that in heart-shaped (HC) and cup-shaped cotyledon (CC) populations, the number of plants with double flowers was much more than that of single flowers and CC seedlings rarely produced single flowers. Therefore, increasing the number of CC seedlings can improve the percentage of double flowers. The highest and lowest numbers of CC seedling were observed in high double and single flower cultivars, respectively. Single flower cultivars showed the maximum count of dumbbell-shaped cotyledons. Chromosome pairing of these cultivars was evaluated using the squash technique. Aneuploid cells were found in 'Nobel' and 'Goddess' cultivars, which showed the highest percentage of CC seedling. Based on morphological measurements, the highest value of inflorescence size was observed in the seedlings with cup-shaped cotyledons.

• Journal of Plant Biotechnology
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• 제47권2호
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• pp.179-184
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• 2020

체세포배 발생과 자엽 형태에 미치는 탄수화물과 삼투제의 영향을 알아보기 위하여 배발생캘러스를 탄소원으로 sucrose와 glucose를 그리고 삼투제로서 mannitol 또는 sorbitol을 3% sucrose와 조합하여 첨가한 MS액체배지에 배양하였다. 플라스크당 체세포배 최대 형성수(128.29)는 glucose단독 또는 삼투제와 3% sucrose를 조합처리한 배지보다 3% sucrose를 첨가한 배지에서 얻을 수 있었고, 그리고 탄수화물과 삼투제의 농도가 증가할수록 체세포배 형성수는 감소하였다. 2개의 자엽을 갖는 전체 체세포배 형성빈도(24.09)는 sucrose 단독(21.52), glucose단독(21.22) 그리고 sorbitol과 3% sucrose의 조합처리보다 mannitol과 3% sucrose조합처리배지에서 약간 높게 나타났다. 특히, sucrose와 glucose농도가 높을수록 2개의 자엽과 트럼펫 모양의 자엽빈도는 점차 감소하였고, 반면 다자엽과 생장이 지연된 구형기 체세포배 빈도는 증가하였다. 한편 삼투제와 3% sucrose의 조합처리 농도가 높을수록 트럼펫 모양과 생장이 지연된 구형기 체세포배 빈도는 뚜렷이 증가하였고, 반면 다자엽 체세포배의 빈도는 점차 감소하였다. 이와 같이 체세포배 발생과 체세포배의 자엽형태는 탄수화물과 삼투제의 처리농도에 의하여 영향을 받을 수 있음을 보여주었다.