• 한국임상보건과학회지
• /
• 제11권1호
• /
• pp.1632-1638
• /
• 2023

Purpose: The aim of this study was to determine the distribution of the cornea thickness and intra ocular pressure Korean adult of 20 to 24 year old using the Pentacam and tonometer. Methods: The subjects of this study were 43 Korean adults with healthy eyes. Corneal thickness was measured with Pentacam device. The intra ocular pressure was measured with tonometer. Data was analyzed by means of the Pearson's correlation cofficient. P-values<0.001 were considered statistically significant. Results: Mean age of subjects was 20.41±0.86 years. The mean +/- intra ocular pressure of the right eye and left eye were 16.236±2.523mmHg and 16.971±1.992mmHg, respectively. The mean central corneal thickness of the right eye and left eye was 545.324±38.682㎛ and 547.442±33.778㎛, respectively. No significant difference in central corneal thickness was found between the right and left eyes. But, there was a statistically significant difference between central cornea thickness and peripheral cornea thickness around 4 mm of central cornea(p<0.001, Pearson's correlation). However there was no statistically significant difference between central cornea thickness and intra ocular pressure. Conclusion: The results of this study could be used as a clinical reference data for diagnosis and treatment of cornea in Korean adult.

• BMB Reports
• /
• 제46권4호
• /
• pp.195-200
• /
• 2013

To understand the corneal regeneration induced by bevacizumab, we investigated the structure changes of stroma and basement membrane regeneration. A Stick soaked in 0.5 N NaOH onto the mouse cornea and 2.5 mg/ml of bevacizumab was delivered into an alkali-burned cornea (2 ${\mu}l$) by subconjunctival injections at 1 hour and 4 days after injury. At 7 days after injury, basement membrane regeneration was observed by transmission electron microscope. Uneven and thin epithelial basement membrane, light density of hemidesmosomes, and edematous collagen fibril bundles are shown in the alkali-burned cornea. Injured epithelial basement membrane and hemidesmosomes and edematous collagen fibril bundles resulting from alkali-burned mouse cornea was repaired by bevacizumab treatment. This study demonstrates that bevacizumab can play an important role in wound healing in the cornea by accelerating the reestablishment of basement membrane integrity that leads to barriers for scar formation.

• 한국임상보건과학회지
• /
• 제1권2호
• /
• pp.31-37
• /
• 2013

Purpose. This study was to investigate the analysis of the central corneal thickness and anterior chamber depth of eyes in Korean Young Population using the Pentacam topography. Methods. The subjects consisted of 126 eyes from normal cornea of Korean aged 7-12($8.857{\pm}1.501$) years during 2009. The thinnest location, pachy apex, and pupil central region of the cornea thickness was measured using the Pentacam pachymetry. The anterior chamber depth of normal corneas was measured with Pentacam. Results. The thinnest location of corneal thickness was $547.413{\pm}37.893$um. The pachy apex of cornea thickness was $552.103{\pm}36.016$um. Also, the pupil center of corneal thickness was measured $552.143{\pm}36.418$um. On the other hand, the mean thickness in the central cornea was a statically significant relationship(t-test, p=0.0002) between pachy apex and thinnest zone. Also the mean thickness of the central cornea was a statically significant correlation(pearson p=0.0001) among the pupil center, pachy apex and thinnest zone. The anterior chamber depth was $3.137{\pm}0.308$mm. The mean depth in the anterior chamber of cornea was a statically relationship(t-test, p>0.05) between OD and OS. Conclusions. The results of this clinical study suggested that the analysis of the central corneal thickness and anterior chamber depth using Pentacam in Korean young population can provide the useful morphological information in diagnosis of cornea for the contact lens fitting and corneal refraction surgery.

• 대한의용생체공학회:의공학회지
• /
• 제40권5호
• /
• pp.143-150
• /
• 2019

To evaluate the toxicity of ophthalmic drug, the Draize test and Bovine Corneal Opacity and Permeability (BCOP) test commonly used. In Draize test, experimental animals were under stress and pain due to long-term exposure of drug. In addition, regarding physiological functions, animal model is not perfectly reflected a human eye condition. Although some models such as $EpiOcular^{TM}$, HCE model, LabCyte Cornea-Model, and MCTT $HCE^{TM}$ were already presented advanced cornea ex-vivo model to replace animal test. In this sense, cornea tissue structure mimicked ex-vivo toxicity model was fabricated in this study. The corneal epithelial cells (CECs) and keratocytes (CKs) isolated from rabbit eyeball were seeded on non-patterned silk film (n-pSF) and patterned silk film (pSF) at $32,500cells/cm^2$ and $6,500cells/cm^2$. Sequentially, n-pSF and pSF were stacked to mimic a multi-layered stroma structure. The thickness of films was about $15.63{\mu}m$ and the distance of patterns was about $3{\mu}m$. H&E stain was performed to confirm the cell proliferation on silk film. F-actin of CKs was also stained with Phalloidin to observe the cytoskeletal alignment along with patterns of the pSF. In the results, CECs and CKs were shown the good cell attachment on the n-pSF and pSFs. Proliferated cells expressed the specific phenotype of cornea epithelium and stroma. In conclusion, we successfully established the ex-vivo cornea toxicity model to replace the eye irritation tests. In further study, we will set up the human ex-vivo cornea toxicity model and then will evaluate the drug screening efficacy.

• 한국안광학회지
• /
• 제3권1호
• /
• pp.201-207
• /
• 1998

눈의 cornea, crystalline의 reflection pattern을 알기 위해 matrix를 이용하여 image의 위치, size를 이론적으로 계산하였고, 3개의 light source와 reflex camera를 이용하여 reflection pattern를 측정할 수 있는 system을 만들었다. 단일 광원을 이용하여 retina에서 반사 pattern으로부터 눈의 원시 및 근시 등을 측정하였고, 2개의 광원을 이용한 reflection pattern으로부터 cornea, crystalline의 curvature를 측정하였다.

• Toxicological Research
• /
• 제35권3호
• /
• pp.287-294
• /
• 2019

The possibility of eye exposure for workers participating in manufacturing of nanoparticles or consumers using products containing nanoparticles has been reported, but toxicity studies on the eye are scarce. In this study, cytotoxicity of five nanoparticles including silver, ceria, silica, titanium and zinc were tested using Statens Seruminstitut Rabbit Cornea (SIRC) cells. When cells were treated with nanoparticles with concentrations of $1-100{\mu}g/mL$ for 24 hr, zinc oxide nanoparticles showed higher toxicity to cornea cells. $LC_{50}$ of zinc oxide nanoparticles was less than $25{\mu}g/mL$ but those of other nanoparticles could not be calculated in this test, which means more than $100{\mu}g/mL$. Generation of reactive oxygen species was observed, and expression of apoptosis related biomarkers including Bax and Bcl-2 were changed after treatment of zinc oxide nanoparticles, while no other significant toxicity-related changes were observed in cornea cells treated with Ag, $CeO_2$, $SiO_2$ and $TiO_2$ nanoparticles.

• 한국안광학회지
• /
• 제16권4호
• /
• pp.439-444
• /
• 2011

목적: 수평축과 수직축 비가 다른 토릭각막의 형상을 수학적인 함수로 표현하여 각막곡률 분포의 분석과 콘택트 렌즈 착용에 필요한 눈물두께 계산에 적용한다. 방법: 각막을 타원함수로 가정하고 함수로부터 유도된 곡률반경식과 각막 토포그라피 측정 곡률반경을 일치시켜 타원함수의 변수를 결정하였다. 계산은 작성한 엑셀프로그램을 사용하였다. 결과: 중심이 각막 정점에 위치하지 않는 리본(나비)형 토포그라피 이미지를 가지는 직난시 각막의 수평, 수직 각 축에 대해 각막의 형상을 수치적으로 알 수 있는 타원함수를 구하였다. 결론: 각막에 대해 고도의 수차 등이 요구되지 않는 응용, 예를 들면 콘택트렌즈 피팅, 난시교정 등에 각막 형상을 단순한 타원함수로 표현하는 것이 편리할 것으로 판단된다.

• 한국안광학회지
• /
• 제8권2호
• /
• pp.1-11
• /
• 2003

확산에 관한 Fick 법칙 및 연속법칙을 사용하여 액체(눈물)-콘택트 렌즈-눈물 층을 통하여 각막으로 유입되는 산소 flux 및 각막 표면의 산소 분압을 계산할 수 있는 일련(一連)의 방정식을 세우고 이들 식에 의거하여 피팅에 따른 눈물 층의 두께, 콘택트 렌즈의 Dk 및 두께 변화가 각막에 유입되는 산소 flux에 어떠한 영향을 미치는가를 예측하였다. 본 모델을 사용하여 적절한 피팅 조건에 부합하고 또한 각막 표면의 산소 분압이 보장되는 콘택트 렌즈의 Dk 및 두께를 결정할 수 있다. 대단히 높은 Dk값을 지닌 콘택트 렌즈라도 너무 flat 또는 tight하게 피팅된 경우 콘택트 렌즈의 주변부와 각막 사이 눈물 층의 간격이 커짐으로 인하여 각막 주변부에 도달하는 산소 flux 및 각막 주변부 표면의 산소 분압이 급격히 감소하게 될 수 있다.

• 한국안광학회지
• /
• 제1권2호
• /
• pp.19-35
• /
• 1996

본 연구는 광학현미경과 전자현미경을 사용해서 자외선을 사용하는 클린벤치에서 각막에 대한 자외선 조사의 영향을 측정하기 위해서 생쥐에 대해 실험을 수행하였다. 결과는 다음과 같다. 대조군에서 각막조직은 비교적 치밀하고, 각 층은 잘 확인되며, 각막의 두께는 일정하였다. 증가하는 실험시간에서 실험의 결과는 매우 차이가 있었다. 초기실험군의 경과들에서는 심하게 변성이 되었다. 그러나 얼마의 기질층들은 종대되고, 얼마의 상피조직은 정상의 모양이 아니었다. 중간실험군들의 결과들은 기질의 심한 종대, 일부지역의 소포, 상피의 응축, 내피의 불규칙한 배열이 있었다. 마지막 실험군의 결과에서는 각막조직의 위축, 내피조직의 종대와 공포화 상피의 부분적인 파괴, 각막조직의 불규칙적인 두께가 있었다.

• 한국임상수의학회지
• /
• 제27권4호
• /
• pp.411-414
• /
• 2010

Rabbit's cornea is the most common thing that used for researches in ophthalmology, so many experimentations have done. The purpose of this study was to survey the corneal pachymetry, tonometry and specular microscopy in New Zealand white rabbits. Three months old male rabbits ($2.9{\pm}0.2\;kg$) were examined with contact the specular microscope to evaluate the endothelial cell density, coefficiency of variation and the percentage of hexagonal cells in the central cornea. For the pachymetry, ultrasonic pachymeter was used for central cornea in each twenty rabbits, and tonometry was performed forty rabbit's eyes to evaluate the intraocular pressure. Corneal endothelial cell density in New Zealand white rabbit was $3304{\pm}354\;cell/mm^2$. Coefficiency of variation was $45.8{\pm}2$ and the percentage of hexagonal cells in the central cornea was $44.4{\pm}11%$. The pachymetry of cornea was $346.1{\pm}21\;{\mu}m$ and tonometry of eye was $14.3{\pm}2\;mmHg$. It is considered that normal New Zealand white rabbit's pachymetry, specular microscopy and tonometry were useful basic data of rabbit's cornea for research and clinic in veterinary ophthalmology.