• Title/Summary/Keyword: copper stress

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An Annealing Control Primer (ACP) System Used for the Isolation and Identification of Copper-Induced Genes in Alfalfa Leaves

  • Lee, Ki-Won;Lee, Sang-Hoon;Kim, Ki-Yong;Ji, Hee Chung;Park, Hyung Soo;Hwang, Tae Young;Choi, Gi Jun;Rahman, Md. Atikur
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.3
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    • pp.237-242
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    • 2016
  • Copper (Cu) is a necessary microelement for plants. However, high concentrations of Cu are toxic to plants that change the regulation of several stress-induced proteins. In this study, an annealing control primer (ACP) based approach was used to identify differentially expressed Cu-induced genes in alfalfa leaves. Two-week-old alfalfa plants (Medicago sativa L.) were exposed to Cu for 6 h. Total RNAs were isolated from treated and control leaves followed by ACP-based PCR technique. Using GeneFishing ACPs, we obtained several genes those expression levels were induced by Cu. Finally, we identified several genes including UDP-glucuronic acid decarboxylase, transmembrane protein, small heat shock protein, C-type cytochrome biogenesis protein, mitochondrial 2-oxoglutarate, and trans-2,3-enoyl-CoA reductase in alfalfa leaves. These identified genes have putative functions in cellular processes such as cell wall structural rearrangements, transduction, stress tolerance, heme transport, carbon and nitrogen assimilation, and lipid biosynthesis. Response of Cu-induced genes and their identification in alfalfa would be useful for molecular breeding to improve alfalfa with tolerance to heavy metals.

A Study on the Dyeing of Ramie Fabric Treated with Medicinal Plant IV. The Natural Dyeing on Ramie Fabric Using Mentha arvensis Herba

  • Seo, Young-Nam;Shin, Gil-Man
    • Plant Resources
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    • v.5 no.3
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    • pp.206-209
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    • 2002
  • This study was performed to investigate the effect of Mentha arvensis Herba extract on the treatment of chromaticity and colorfastness. Mentha arvensis Herba has been used as a Korean medicine. It is effective in headache and stress. It is also good as a aphtha and in treating cold. In the long history of Korea, dyeing has been applied for a means representing the grace of natural and inner esthetic consciousness of man. Vegetable dyes give us such great benefits, diversified color, but no pollution. And ramie fabric has distinctive features such as beautiful brilliance, elegance, and strong durability. So, it is regarded as a special product of Korea traditionally. These studies were carried out to treat with acetate iron, dichloride copper and alum with a mordant to ramie fabric. The ramie fabric was died with Mentha arvensis Herba extract. The results of experiment showed as follows: First, the chromophoric degree was the highest in acetate iron but not distinction in another mordants. Second, the light colorfastness was the highest in non treated and dichloride copper, but alum was the lowest. Third, the discoloration was alum and dichloride copper showed first grade in washing colorfastness. Abrasion colorfastness was not significant in this test. According the previous results, Mentha arvensis Herba has an efficiency in removing aphtha and in treating cold. So it is considered that Mentha arvensis Herba can be applied effectively to headache and stress.

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Tensile Tests for Copper Thin Foils by Using DIC Method (DIC 법을 이용한 구리박막의 인장시험)

  • Kim, Chung Youb;Song, Ji Ho;Park, Kyung Jo
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.36 no.12
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    • pp.1529-1534
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    • 2012
  • In this study, tensile tests for 12-${\mu}m$-thick copper thin foils were performed by using the DIC method. The DIC method provided precise stress-strain curves for thin film materials, and a commercial inkjet printer can be simply and effectively used for printing speckle patterns on the specimen of Cu thin films whose surface contrast is too low to apply the DIC method. The mechanical properties of Cu thin foils obtained in this study are as follows: elastic modulus E = 89.2 GPa, 0.2% offset yield stress $S_{0.2%}$= 232.8 MPa, tensile strength $S_u$= 319.2 MPa, elongation at fracture ${\varepsilon}_f$=16.8 %, and Poisson's ratio ${\nu}$= 0.34.

Effect of Cu-resistant Pseudomonas on growth and expression of stress-related genes of tomato plant under Cu stress (구리-오염 토양에서 토마토 식물의 생장과 스트레스-관련 유전자 발현에 미치는 구리-내성 Pseudomonas의 영향)

  • Kim, Min-Ju;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.53 no.4
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    • pp.257-264
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    • 2017
  • Pseudomonas veronii MS1 and P. migulae MS2 have several mechanisms of copper resistance and plant growth promoting capability, and also can alleviate abiotic stress in plant by hydrolysis of a precursor of stress ethylene, 1-aminocyclopropane-1-carboxylic acid (ACC) by ACC deaminase. In 4-week pot test for tomato growth in soil contained 700 mg/kg Cu, inoculation of MS1 and MS2 significantly increased root and shoot lengths, wet weight and dry weight of tomato plants compared to those of uninoculated control. The inoculated tomato plants contained less amounts of proline that can protect plants from abiotic stress, and malondialdehyde, an oxidative stress marker than those of control. ACC synthase genes, ACS4 and ACS6, and ACC oxidase genes, ACO1 and ACO4, both involved in ethylene synthesis, were strongly expressed in Cu stressed tomato, whereas significantly reduced in tomato inoculated with MS1 and MS2. Also, a gene encoding a metal binding protein metallothionein, MT2 showed similar expression pattern with above genes. All these results indicated that these rhizobacteria could confer Cu resistance to tomato plant under Cu stress and allowed a lower level of Cu stress and growth promotion.

Manufacturing Process Effect on Fatigue Properties for Copper Thin Film (구리박막의 피로특성에 관한 제조공정의 영향)

  • An, Joong-Hyok;Park, Jun-Hyub;Kim, Yun-Jae
    • Proceedings of the KSME Conference
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    • 2007.05a
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    • pp.1783-1786
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    • 2007
  • The copper film coated by Sn is often used in various applications such as LCD, Mobile Phone and etc. Especially, when the film is used as tape carrier package(TCP) of LCD panel, the film is repeatedly applied by mechanical or(and) thermal stress and then is often failed. Therefore, to guarantee the reliability of the electrical devices using the film, the tensile and fatigue characteristics of the film are important. In this study, to obtain the tensile and fatigue characteristics of the film, the specimen was fabricated by etching process to make a smooth specimen of 2000 ${\mu}m$ width, 8000 ${\mu}m$ length and 15.26 ${\mu}m$ thickness. The 2 kinds of specimen were fabricated by other manufacturing process. These specimens had values of Young's modulus(80.2GPa) lower than literature values(108${\sim}$145GPa) for bulk values, but had high values of the yield and ultimate strength as 317MPa and 437MPa, respectively. And fatigue test of load-control with 20Hz frequency was performed.

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Direct Sealing Glass-Ceramics to Metal (직접 결합방법에 의한 Glass-Ceramics과 금속의 접합)

  • Kim, Hwan;Lee, Ki-Kang
    • Journal of the Korean Ceramic Society
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    • v.18 no.2
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    • pp.99-104
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    • 1981
  • Glass-ceramics possessed a number of characteristics which suggested their suggested their use for sealing to metals. The choice of particular glass-ceramics compositions for this application is governed by various factors, including workability of the glasses, thermal expansion characteristics and the matching of these to appropriate metals. Other properties, such as mechanical strength, determined the performance of glass-ceramics to metal seals. The purpose of the present study was to investigate direct sealing behaviour of copper to $Li_2O-ZnO-SiO_2$ system glass-ceramics. The design of the seal was a concentric seal which might contribute to the strong bond formation by providing compressive stress during thermal excursions. Tensile strengths of sealing layers were measured by Instron test machine. The layers were examined by electron probe microanalyzer. Crsystallization rate was increased with the amount of ZnO or $Li_2O$, and ZnO increased the sealing strength, but $Li_2O$ lowered it. Sealing mechanism was due to the formation of metal oxides, which acted as binder between copper and glass-ceramics. The nickle-plated copper seal with 10% $Li_2O$ and 30% ZnO was the most strong seal, and its sealing strength was more than 56kg/$\textrm{cm}^2$.

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Role of Proline Accumulation in Response to Toxic Copper in Microcystis aeruginosa

  • Park, So-Hyun;Hong, Jung-Hee
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.10 no.S_4
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    • pp.189-196
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    • 2001
  • The blue green alga, Microcystis aeruginosa, was found to accumulate proline under the stressful concentration of cupric ions. The changes of proline level in Microcystis aeruginosa in response to copper(Cu) have been monitored and the function of the accumulated proline was studied with respect to its effect on Cu uptake. Exposure of Microcystis aeruginosa elevated concentrations of Cu led to accumulation of fee proline depending on the concentrations of the metal in the external medium. The greater the toxicity or accumulation of the metal, the higher the amount of proline in algal cells were found. When proline was exogenously supplied prior to Cu treatment, the absorption of Cu was markedly reduced. When exogenous proline was supplied after Cu treatment, it resulted in a remarkable desorption of the adsorbed Cu immediately after the addition of proline. Pretreatment of Microcystis aeruginosa with proline counteracted with metal-induced lipid peroxidation. The results of the present study showed a protective elect of proline on metal toxicity through inhibition of lipid peroxidation and suggested that the accumulation of proline may be related to the tolerance mechanism for dealing with Cu stress.

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Forming Simulation of EV Motor Hairpin by Implementing Mechanical Properties of Polymer Coated Copper Wire (고분자 필름 및 구리선 이종 물성을 고려한 EV모터용 헤어핀 성형 공정 해석)

  • D. C. Kim;Y. J. Lim;M. Baek;M. G. Lee;I. S. Oh
    • Transactions of Materials Processing
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    • v.32 no.3
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    • pp.122-128
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    • 2023
  • As electric vehicles (EV) have increasingly replaced the conventional vehicles with internal combustion engines (ICE), most of automotive makers are actively devoting to the technology development of EV parts. Accordingly, the manufacturing process for power source has been also shifting from engine/transmission to EV motor/reducer system. However, lack of experience in developing the EV motor still remains as a technical challenge. In this paper, we employed the forming simulation based on finite element modeling to solve this problem. In particular, in order to increase the accuracy of the forming simulation, we introduced the elastic-plastic constitutive model parameters for polymer-copper hybrid wire by investigating the individual strain-stress curves, and elastic modulus of polymer and copper. Then, the reliability of modeling procedure was confirmed by comparing the simulated results with experiments. Finally, the identified mechanical properties and finite element modeling were applied to a hairpin forming process, which involves multiple deformation paths such as bending, pressing, widening, and twisting. The proposed numerical approach can replace common experience or experiment based trials by reducing production time and cost in the future.

고효율 저가형 결정질 실리콘 태양전지에 적용될 Ni/Cu 전극 및 Ni silicide 형성에 대한 연구

  • Kim, Min-Jeong;Lee, Su-Hong
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2009.11a
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    • pp.260-260
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    • 2009
  • In high-efficiency crystalline silicon solar cell, If high-efficiency solar cells are to be commercialized, It is need to develop superior contact formation method and material that can be inexpensive and simple without degradation of the solar cells ability. For reason of plated metallic contact is not only high metallic purity but also inexpensive manufacture. It is available to apply mass production. Especially, Nickel, Copper are applied widely in various electronic manufactures as easily formation is available by plating. Ni is shown to be a suitable barrier to Cu diffusin as well as desirable contact metal to silicon. Nickel monosilicide has been suggested as a suitable silicide due to its lower resistivitym lower sintering temperature and lower layer stress than $TiSi_2$. In this paper, Nickel as a seed layer and diffusion barrier is plated by electroless plating to make nickel monosilicide.

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Determination of Blow Efficiency of the Forging Hammer (단조해머의 타격효율 결정)

  • 이성호;조남춘;이종수
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.19 no.6
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    • pp.1539-1544
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    • 1995
  • Copper blow test to measure the forging capability of 35 ton counterblow hammer and upset of plasticine on the model hammer to investigate the change of the blow efficiency during the forging process have been performed together with finite element analyses of these experiments. The blow efficiency of the hammer has been found to be dependent on the friction and on the contact area between the die and the workpiece. The effects of the volume and the aspect ratio of the billet have not been found. Inferring from the experimental results and Schey's empirical formula on the forging load, we expect that the efficiency also varies with the flow stress of the workpiece material and with the shape complexity of the forging product.