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Cell Fusion Between Xylose Fermenting Yeast and Xylanase Secreting Yeast (Xylanase 분비효모와 Xylose 발효효모의 Protoplast Fusion)

  • 김남순;배명애;서정훈
    • Microbiology and Biotechnology Letters
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    • v.17 no.2
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    • pp.88-93
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    • 1989
  • To improve a new yeast strain capable of converting xylan to ethanol directly, we tried protoplast fusion between xylose fermenting yeast (Candida sp. X-6-41) and xylan assimilating yeast (Crypto-coccus sp. XB-33), finally selected the most promising two fusants (XFU-1 and XFU-2). As the optimum conditions for protoplast formation, the yeast cells were cultured to exponential phase in YPD and YPX containing 0.6M KCI, respectively, and then treated with zymolyase (0.25mg/$m\ell$), cellulase(4mg/$m\ell$) and 100mM 2-mercaptoethanol at pH 8 and 3$0^{\circ}C$. The protoplasts of parental auxotrophs were fused in the presence of 20mM CaCl$_2$and 40% polyethylene glycol(M.W.4000). The physiological and morphological characteristics of the fusants, such as assimilation of carbon sources, cell size, growth rate, xylanase activity and xylan fermentation ability were investigated. Xylanase activity of fusants that cultured in chemically minimal medium was higher than that of fusants that cultured in completed medium, because xylanase producing activity of xylose fermenting yeast(X-6-41) was inhibited by isoleucine.

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Outdoor Workers' Use of Sun Protection at Work and Leisure

  • Peters, Cheryl E.;Koehoorn, Mieke W.;Demers, Paul A.;Nicol, Anne-Marie;Kalia, Sunil
    • Safety and Health at Work
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    • v.7 no.3
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    • pp.208-212
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    • 2016
  • Background: Outdoor workers are at risk of high ultraviolet radiation exposure, and may have difficulty using sun protection. The objectives were to determine the prevalence of sun protection behaviors in a sample of outdoor construction workers, and to assess which factors predict better sun protection practices. Methods: Participants were recruited via construction unions. Workers answered a questionnaire on demographics, skin cancer risk, sun protection behaviors, and job. Sun protection behavior scores (from questions on sunscreen use, sleeved shirt, hat, shade seeking, sunglasses) were calculated by converting Likert-scale answers to scores from 0 to 4, and taking the mean (separately for work and leisure). Determinants of sun protection behavior scores were examined for work and leisure using generalized linear models. Results: Seventy-seven workers had complete questionnaire data (participation 98%). Sun protection behaviors used most often were hats (79% often/always) and sleeved shirts (82% often/always); least prevalent were shade-seeking (8% often/always) and sunscreen (29% often/always). For both work and leisure scores, the strongest predictor was skin type, with fairer-skinned individuals having higher sun protection behavior scores. Workers had higher scores at work than on weekends. Workplaces that required hats and sleeved shirts for safety purposes had higher protection behavior scores. Conclusion: This high-participation rate cohort helps characterize sun protection behaviors among outdoor workers. Workers practiced better sun protection at work than on weekends, suggesting that workplace policies supportive of sun protection could be useful for skin cancer prevention in the construction industry.

Influence of Isoflavone Intake and Equol-producing Intestinal Flora on Prostate Cancer Risk

  • Sugiyama, Yukiko;Masumori, Naoya;Fukuta, Fumimasa;Yoneta, Akihiro;Hida, Tokimasa;Yamashita, Toshiharu;Minatoya, Machiko;Nagata, Yoshie;Mori, Mitsuru;Tsuji, Hirokazu;Akaza, Hideyuki;Tsukamoto, Taiji
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.1
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    • pp.1-4
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    • 2013
  • Background: The age-adjusted incidence rate of prostate cancer (PCa) has been reported to be lower among Asians than Western populations. A traditional Japanese meal, high in soybean products or isoflavones, may be associated with a decreased risk of PCa. Equol, which is converted from daidzein by human intestinal flora, is biologically more active than any other isoflavone aglycone. Materials and Methods: We reviewed not only recent epidemiological studies on association of isoflavones with PCa risk, but also recent research on human intestinal bacteria responsible for converting daidzein into equol. Studies were systematically searched from the database published within the last 5 years of from 2008-2012. Results: Five out of 6 articles showed significant association of isoflavones with a decreased risk of PCa, and two of them consistently showed that equol-producers carry a significantly reduced risk of PCa. Furthermore, 5 human intestinal bacteria that can convert daidzein into equol were identified in the last 5 years. Conclusions: If equol can reduce risk of PCa, a possible strategy for reducing the risk of PCa may be to increase the proportion of equol-producers by changing the intestinal flora to carrying an equol-producing bacterium with dietary alteration or probiotic technology.

Enhanced ART1 Algorithm for the Recognition of Student Identification Cards of the Educational Matters Administration System on the Web (웹 환경 학사관리 시스템의 학생증 인식을 위한 개선된 ART1 알고리즘)

  • Park Hyun-Jung;Kim Kwang-Baek
    • Journal of the Korea Society of Computer and Information
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    • v.10 no.5 s.37
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    • pp.333-342
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    • 2005
  • This paper proposes a method, which recognizes student's identification card by using image processing and recognition technology and can manage student information on the web. The presented scheme sets up an average brightness as a threshold, based on the brightest Pixel and the least bright one for the source image of the ID card. It is converting to binary image, applies a horizontal histogram, and extracts student number through its location. And, it removes the noise of the student number region by the mode smoothing with 3$\times$3 mask. After removing noise from the student number region, each number is extracted using vertical histogram and normalized. Using the enhanced ART1 algorithm recognized the extracted student number region. In this study, we propose the enhanced ART1 algorithm different from the conventional ART1 algorithm by the dynamical establishment of the vigilance parameter. which shows a tolerance limit of unbalance between voluntary and stored patterns for clustering. The Experiment results showed that the recognition rate of the proposed ART1 algorithm was improved much more than that of the conventional ART1 algorithm. So, we develop an educational matters administration system by using the proposed recognition method of the student's identification card.

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The Relationshin between ACE Inhibitory Activity and Degradations of Sulfur Containing Materials in Dolsan Leaf Mustard Juice

  • Yoo Eun-Jeong;Choi Myeong-Rak;Lim Hyun-Soo
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.5
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    • pp.400-404
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    • 2004
  • This Study was tarried out to investigate the relationship ACE inhibitory activity and degradations of sulfur containing materials in Dolsan leaf mustard juice (DLMJ). The changes of sulfur containing materials which were treated with autolysis, myrosinase, ascorbate and papain were studied, as well as the changes of ACE inhibitory activity in DLMJ. At $37^{\circ}C$, sulfur contain-ing materials by autolysis decreased most rapidly from $0.43\%$ to $0.13\%$ in the second day. Conversely. ACE inhibitory activity increased most from $66\%$ to $87\%$. in the second day at $37^{\circ}C$. As myrosinase concentrations increased more, sulfur containing materials in DLMJ decreased more. The ACE inhibitory activities at 0, 0.5, 1, 2, and 4 Units of myrosinase for 240 min later were 70, 74, 75, 82, and $85\%$, respectively. At 1 mM ascorbate. concentrations of Sulfur containing materials in DLMJ decreased more significantly on the second day than on the other days. At 1 mM ascorbate for 6 days, ACE Inhibitory activity reached a maximum of about $92\%$. And, an increase of papain concentration was noted in accordance with a decreased sulfur containing materials. The maximum rate of AEC inhibitory activity at control, 3, 6, and 12 Units of papains treatments was shown as 70, 70, 75, and $78\%$ at 60 min, respectively. These results suggested that the degradation of sulfur containing materials led to the increase of ACE inhibitory activity. Consequently, it was suggested that ACE inhibiting was significantly related to the degradatives of sulfur containing materials.

A Study on the Connection Method for the Collapse Damage of Electric Power Facilities due to Earthquake Effects (지진 영향으로 인한 전기시설물의 붕괴피해 연계 방안 연구)

  • Lee, Byung-Hoon;Lee, Byung-Jin;Oh, Seung-Hee;Jung, Woo-Sug;Kim, Kyung-Seok
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.18 no.5
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    • pp.203-208
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    • 2018
  • In this paper, we selected power and power distribution facilities corresponding to urban infrastructure from the types of damage that could be caused by earthquakes and studied how they were calculated to damage. To calculate the damage, a graph of the magnitude of the damage was produced by applying the vulnerability curve calculation formula, which can be calculated for each type and type of facility. The scale of the earthquake and the probability of the occurrence of damage by the maximum earthquake acceleration were shown in the form of a vulnerability rate when the earthquake occurred in the urban infrastructure facility for utilizing the calculation result. It also applied a method of quantifying the fragility, which is a method of converting the calculated fragility into an integrated form, to represent a constant value for the magnitude of the damage. Continuing research, such as the method applied in this paper, could help identify in advance the types of structures affected by an earthquake and respond to reducing damage.

Research on the phenomenon of sick house syndrome and how to remove harmful gases (새집증후군 현상 및 유해가스 제거방안 연구)

  • Choe, Yoowha
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.3
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    • pp.449-456
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    • 2020
  • When you move to a new home, or when you change the wallpaper or flooring of your home, office, etc., you can enjoy the joy of opening your new home with the sick house syndrome, such as the stinging smell and stinging eyes that may appear after the interior work. It is only a moment. Volatile organic compounds from building materials, adhesives, wallpaper, and paints used in new buildings or new furniture cause residents' health and discomfort in indoor life. These volatile organic compounds include benzene, toluene, acetone, and styrene, as well as the representative formaldehyde, and these substances are slowly released over a long period of time, causing acute or chronic diseases to residents. As a method for removing organic volatile substances, physical methods using adsorption, chemical methods for converting volatile substances to other substances, or a mixture of the two are mainly used. In this paper, a sustained release chlorine dioxide gel pack obtained by a method for controlling the reaction rate of a reactant and the release of a product is mixed with a zeolite adsorbent having an optimized hole diameter to adsorb and decompose and remove formaldehyde suspended in the air. I would suggest an effective method.

Anaerobic Treatment of Landfill Leachate Using a Upflow Anaerobic Sludge Blanket Reactor (UASB 반응조를 이용한 매립지 침출수의 혐기성 처리)

  • Lee, Chae-Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.14 no.4
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    • pp.151-160
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    • 2006
  • Anaerobic treatment of landfill leachate was studied to investigate the behaviors of pollutant and the characteristics of microorganism for 10 months. The upflow anaerobic sludge blanket (UASB) reactor achieved about 90% chemical oxygen demand (COD) removal at organic loading rates(OLR) up to $20kgCOD/m^3.d$. At higher OLR ($8-20kgCOD/m^3.d$), the propionate concentration increased, indicating that converting propionate to acetate was the rate-limiting step. Nevertheless, increase in the precipitate inside and on the surface of granules as well as on the wall of the reactor resulted in operational problems. The main inorganic precipitate in the granule was calcium compound. Although specific methanogenic activity (SMA) was not affected seriously in this study, metals had to be removed prior to anaerobic treatment so as to be free from the excessive inorganic accumulation that resulted in operational problems.

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The Study of Luppe Smelting with Converting Dust and Slag (제강전로 더스트와 슬래그를 이용한 루페제련에 관한 연구)

  • 황용길;이상화;김재일;김연수
    • Resources Recycling
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    • v.7 no.2
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    • pp.39-45
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    • 1998
  • We smelted thc pellets made by mixing the distilled carbon from wlISte Lires, LD converter dust and slag with reduction process in the revcrberatory furnace. Thc obtained results are as follows 1) The removal mte of zinc appears above 97% after T reducing the pellets at $1300^{\circ}C$ for Ihr and the zinc content in the residue are 0.1~D.2%. 2) Under the mixing condition of 500 g LD dust. 150-200 g LD slag and 30-50 g distilled carbon of waste lires the removal raho of zinc shows above 95%, while t the 50-60% Fe remains in the residue. 3) After smelting at $1350^{\circ}C$ for 3hrs, the recovery ratio of pig iron reduced from lhe p pellets containing 15-20% LD slag and 4.1-7.2% distilled carbon of waste tires appears in the range of 89.3-92%. 4) Tbe c chemical composition of the recovered pig iron is 1.7%C, O.05%P, 0.05%S and balance Fe. 5) Tbe recovered dust from the d dust collcctor alter finishing the reduction rcaction appears as a crude zinc oxide conLaining 60% zinc.

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Collision Avoidance Maneuver of Unmanned Aerial Vehicles Applying TCAS-II (TCAS-II를 응용한 무인항공기의 충돌회피기동 연구)

  • Jo, Sin-Je;Kim, Jong-Seong;Jang, Dae-Su;Tak, Min-Je;Gu, Hwon-Jun
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.34 no.4
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    • pp.33-39
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    • 2006
  • In this paper, the collision avoidance of Unmanned Aerial Vehicles(UAVs) by applying the Traffic alert and Collision Avoidance System II(TCAS-II) is introduced. The performances of two UAVs whose maximum vertical rates are different each other are compared and analysed by not only converting many TCAS-II commands into an autopilot input but also implementing a computer program based on the Minimum Operational Performance Standards for TCAS-II. As the alternative to a possible Near Mid-Air Collision for UAVs whose maximum vertical rates are low, we have proposed a modified algorithm considering the maximum vertical rate and altitude. The modified algorithm is available on the assumption that a wider surveillance range is provided by a ADS-B system.