• Title/Summary/Keyword: conventional pig

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Radiolabelled Monoclonal Antibodies (McAb): An Alternate Approach to the Conventional Methods for the Assessment of Cardiomyocyte Damage in an Experimental Brain-Death Pig Model

  • Haider, Kh.H.;Stimson, W.H.
    • Archives of Pharmacal Research
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    • v.21 no.5
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    • pp.496-502
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    • 1998
  • The present study was carried out to determine the possible use of cTn-I in the cardiac myofibrillar architecture, as a potential target for in vivo radioimmunodetection of cardiac damage in a brain death pig model. Radioiodiantion of the anti-cTn-I 5F4 McAb was carried out by lactoperoxidase method. the percentage iodine incorporation achieved was 70-75%. The radioiodinated McAbs were purified on Sephadex G-25 column and characterised by Paper chromatography, Phast Gel electrophoresis and electroimmunoblotting. Radioiodinated anticTn-I 5F4 McAbs were employed alongside Pyrophosphate($Tc_{99m}$-PPi$) and $Thallium^{201}$ chloride($TI^{201}$) in 24 landrace pigs (brain-dead=18 & sham-operated=6). The percentage cardiac uptake of the radiolabelled antibody injected dose was significantly higher in the brain dead animals(0.196%) as compared to that of sham-operated animals (0.11%). Specific in vivo localization of radiolabelled McAbs in the infarcted cardiac tissue was confirmed by computer-aided reconstruction of 3-D images of the isolated heart. The preliminary results of the study revealed preferential uptake of radiolabelled antibody at the site of myocyte damage resulting from artificially induced brain death.

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Development of serodiagnostic surface plasmon resonance imaging assay for the detection of antibodies to porcine circovirus type 2

  • Park, Chul;Kim, Bum-Seok;Kim, Yong-Hwan;Cho, Ho-Seong
    • Korean Journal of Veterinary Service
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    • v.34 no.1
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    • pp.1-4
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    • 2011
  • A surface plasmon resonance imaging (SPRI) assay was developed for measuring porcine circovirus type 2 (PCV2) antibody using a recombinant capsid protein as an antigen. The diagnostic potential of SPRI for detecting antibodies to the PCV2 capsid protein was compared with that of a conventional enzyme-linked immunosorbent assay (ELISA) using 70 pig serum samples taken from 6 pig farms. There was a strong positive correlation between the SPRI and ELISA (n = 70, r = 0.911, P<0.01). Therefore, this recombinant capsid protein can be used as an antigen for serological studies, and the SPRI, a label-free and high-throughput method, is expected to be a valuable tool in the serodiagnosis of PCV2 infection.

Pipeline Defects Detection Using MFL Signals and Self Quotient Image (자기 누설 신호와 SQI를 이용한 배관 결함 검출)

  • Kim, Min-Ho;Rho, Yong-Woo;Choi, Doo-Hyun
    • Journal of the Korean Society for Nondestructive Testing
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    • v.30 no.4
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    • pp.311-316
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    • 2010
  • Defects positioning of underground gas pipelines using MFL(magnetic flux leakage) inspection which is one of non-destructive evaluation techniques is proposed in this paper. MFL signals acquired from MFL PIG(pipeline inspection gauge) have nonlinearity and distortion caused by various external disturbances. SQI(self quotient image), a compensation technique for nonlinearity and distortion of MFL signal, is used to correct positioning of pipeline defects. Through the experiments using artificial defects carved in the KOGAS pipeline simulation facility, it is found that the performance of proposed defect detection is greatly improved compared to that of the conventional DCT(discrete cosine transform) coefficients based detection.

Defect Length Estimation Using SQI for Underground Gas Pipelines (SQI를 이용한 지하 매설 가스 배관 결함 길이 추정)

  • Kim, Min-Ho;Choi, Doo-Hyun
    • Journal of the Korean Institute of Gas
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    • v.15 no.2
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    • pp.27-32
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    • 2011
  • In this paper a new defect length estimation algorithm using SQI(self quotient image) is presented for the MFL(magnetic flux leakage) inspection of underground gas pipelines. Gas pipelines are magnetized by the permanent magnets of the MFL PIG(pipeline inspection gauge) when the PIG runs through pipelines. If defects or corrosions exist in the pipeline, magnetic leakage flux is increased. The MFL signals measured by hall sensors are analyzed to estimate defect length using SQI. For 74 real defects carved in KOGAS pipeline simulation facility(KPSF) the accuracy of defect length estimation of the proposed algorithm was compared with that of conventional methods.

Evaluation of Ventilation Rate and External Air Mixing Ratio in Semi-closed Loop Ventilation System of Pig House Considering Pressure Loss (압력손실을 고려한 양돈시설의 반폐회로 환기시스템의 환기량 및 혼합비율 평가)

  • Park You-me;Kim Rack-woo;Kim Jun-gyu
    • Journal of The Korean Society of Agricultural Engineers
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    • v.65 no.1
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    • pp.61-72
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    • 2023
  • The increase in the rearing intensity of pigs has caused deterioration in the pig house's internal environment such as temperature, humidity, ammonia gas, and so on. Traditionally, the widely used method to control the internal environment was through the manipulation of the ventilation system. However, the conventional ventilation system had a limitation to control the internal environment, prevent livestock disease, save energy, and reduce odor emission. To overcome this problem, the air-recirculated ventilation system was suggested. This system has a semi-closed loop ventilation type. For designing this system, it was essential to evaluate the ventilation rates considering the pressure loss of ducts. Therefore, in this study, pressure loss calculation and experiment were conducted for the quantitative ventilation design of a semi-closed loop system. The results of the experiment showed that the inlet through which external air flows should always be opened. In addition, it was also found that for the optimum design of the semi-closed loop ventilation system, it was appropriate to install a damper or a backflow prevention device rather than a ventilation fan.

Comparison of Stress Indicators in Blood and Muscle of Pigs in Conventional and Animal Welfare Farms (일반 및 동물복지농장 돼지의 혈액 및 근육 내 스트레스 인자 비교)

  • Lee, Jeong-Eun;Park, Jin-Ryong;Kang, Da-Rae;Kim, Hee-Eun;Nam, Ki-Chang;Shim, Kwan-Seob
    • Korean Journal of Organic Agriculture
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    • v.28 no.4
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    • pp.627-641
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    • 2020
  • Intensive farming methods that do not guarantee animal welfare can induce stress in pigs. Stress, in turn, can reduce their disease resistance and influence their hormones and metabolites in such a manner that productivity is negatively affected. This study was conducted to compare the stress related factors and blood characteristics of pigs raised on conventional farms and those raised on animal welfare farms. We measured the levels of cortisol, epinephrine and norepinephrine, biochemical parameters in blood and glycogen, L-lactate and heat shock protein 70 (HSP70) in muscle, as physiological markers of indicating the stress in conventional farm pigs (Control, n=10) and animal welfare farm pigs (Welfare, n=10). We found that there was a significant difference in the albumin-globulin ratio (A/G ratio), as well as the albumin (ALB), blood urea nitrogen (BUN) and aspartate aminotransferase (AST) levels between the two farms. Epinephrine was significantly higher in conventional farm, while level of norepinephrine was higher in animal welfare farm. There was no significant difference in cortisol, which is known as a stress hormone, across the two groups of farms. Muscular glycogen content was significantly high in animal welfare farm pigs. While L-lactate tended to be low in the animal welfare farm pigs, the difference between them and the conventional farm cohorts was not significant. HSP70 showed high levels of expression in conventional farm. Thus, we suggested that blood parameter results showed a stress response in the livers of conventional farm, and that catecholamine hormones, glycogen, L-lactate and HSP70 can be used as physiological factors of assessing animal welfare.

Comparison of Weekly and Batch Management System for Sows (모돈의 주간관리와 그룹관리 비교)

  • Jang, Young-Dal;Ju, Won-Seok;Long, Hong-Feng;Piao, Long-Guo;Jang, Sung-Kwon;Chung, Chung-Soo;Kim, Yoo-Yong
    • Journal of Animal Environmental Science
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    • v.15 no.2
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    • pp.171-182
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    • 2009
  • Conventionally, many pig producers have utilized a continual sow managements system that the matings, farrowings and weanings are done weekly basis. But this transitional method is not able to cut the cycle of diseases and fully apply all-in/all-out system because of the continuous flow of sows and pigs. Conventional weekly management system is currently limiting in small farm to work efficiently both for workers and pigs. Therefore, pig producers have found novel management methods for applying all-in/all-out system, improving pig health, leading to better growth, lowering mortality and reducing medication costs nowadays. Moreover, all-in/all-out pig management system has known as a strategy for improving productivity in swine farm. The batch system is one of the best management methods to adopt all-in/all-out pig management system that prevent spreading diseases in pig and remove cycle of diseases. Batch farrowing system is a concept for providing a group of sows that delivery within a specific farrowing interval and inducing a large enough scale of piglets to fill the weaner facilities. There are different types of batch farrowing system with batch size and interval of farrowing when several factors at the swine farm are considered such as total number of sows, available facilities in the farm, and the efficiency of workforce. Sow managements such as farrowing, weaning and breeding, every 3 weeks rather than weekly, 2 or 5-week interval have advantages for workers and reproductive cycle of sows as well as pig flow. Because there are several pros and cons both in weekly and batch management system, various factors should be considered to apply the most suitable management system in each individual farm. To improve poor swine productivity in Korea compared to ED, batch system for sows will be an alternative choice which is able to prevent high incidence of diseases in swine farm such as PMWS, PRRS, PRDC and PED because all-in/all-out pig management can be also applied automatically by using this management system.

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The Search of Pig Pheromonal Odorants for Biostimulation Control System Technologies: Ⅱ. Holographic QSAR Model for Binding Affinities between Ligands of Volatile Odorants Molecules and Porcine Odorant Binding Protein (pOBP) (생물학적 자극 통제 수단으로 활용하기 위한 돼지 페로몬성 냄새 물질의 탐색: Ⅱ. 휘발성 냄새분자의 리간드와 Porcine Odorant Binding Protein (pOBP) 사이의 결합 친화력에 관한 홀로그래피적 QSAR 모델)

  • Sung N. D.;Park C. S.;Choi Y. S.;Myung P. K.
    • Reproductive and Developmental Biology
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    • v.29 no.1
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    • pp.43-48
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    • 2005
  • To search of a new porcine pheromonal odorants for biostimulation control system technologies to offer a potentially useful and practical way to improve reproductive efficiency in livestock species, the holographic quantitative structure activity relationship (HQSAR) model between odorants, 2-phenoxytetrahydrofurane (A), 2-cyclohexyl-oxytetrahydrofurane (B), derivatives and binding affinity constants (p[Od.]/sub 50/) for porcine odorant-binding protein (pOBP) as receptor of pig pheromones were derivated and disscused. The binding affinity constants of cyclohexyl substituents (A) for pOBP were higher (A>B) than that of phenyl substituents (B). It was revealed that the optimum HQSAR model XI using PLS analyses had a fragment length (5∼8) with chirality at 5 components and hologram length 97 bin, which had a cross-validated q²(predictablities) of 0.916, and a conventional correlation coefficient r² (fitness) of 0.988, respectively. From the atomic contribution, the C3 and C5 atom in 2-oxyfuryl group contributed to binding affinity constants, whereas the central carbon atom in tert-butyl group on the cyclohexyl ring and the C4 atom of furyl group parts showed no contribution.

Old Service Pipe Cleaning of Polly-pigs Cleaning technique (Polly-pigs를 이용한 노후급수관의 세관에 관한 연구)

  • Lee, Hyun-Dong;Bae, Chul-Ho;Park, Jeong-Hoon;Kim, Gil-Nam
    • Journal of Korean Society on Water Environment
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    • v.18 no.3
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    • pp.303-312
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    • 2002
  • Polly-pigs technique was developed to remove internal rusts and scales from service pipes in the building by using Polly-pigs that were used as various applications in conventional plant pipelines. Results of cleaning experiments on 15mm GSP(Galvanic Steel Pipe) showed that hydraulic cross sectional area was more increased from 3.5% to 15.4% for straight part or elbow parts of several test pieces. Results of optical analysis also showed that rust and scale removed mostly by KDP series(only consisted of Polyurethane) were outside red colored scale ($Fe_2O_3{\cdot}H_2O$), and there was a limitation to the removal of black colored scale($Fe_2O{\cdot}nH_2O$) that was below red colored scale. But it was evaluated that KDPS series coated fine sand with KDP series could remove not only red colored scale but also black colored scale and more increase hydraulic cross sectional areas of 15mm GSP old service pipes from 13.0% to 17.9%. After KDPS series cleaning, hydraulic cross sectional areas of them were recovered from 95.9% to 99.5%. Turning force of Polly-pigs was largely improved by the effect of Helical guide vane(Cleaning v/v) and Rotating wing(Pigs), and the number of pig rotating also more increased sixteen times compared with conventional cleaning system without Helical guide vane and Rotating wing. After KDPC series cleaning of 100mm GSP old service pipes that hydraulic cross sectional areas were 90%, hydraulic cross sectional areas were almost recovered perfectly like new service pipes. Additionally pressure necessary to run Polly-pigs m 100mm GSP was lower and cleaning efficiency also was higher than 15mm GSP cleaning. Therefore it was thought that as the diameter of pipe was more increased, pressure necessary to clean service pipes was more decreased and cleaning efficiency was more increased in Polly-pigs cleaning.

Effects of Ventilation Systems on Interior Environment of the Growing-finishing Pig House in Korea (육성$\cdot$비육돈사 내에서 환기형태별 환경조사 연구)

  • Song J. I.;Yoo Y. H.;Jeong J. W.;Kim T. I.;Choi H. C.;Kang H. S.;Yang C. B.;Lee Y. Y.
    • Journal of Animal Environmental Science
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    • v.10 no.2
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    • pp.93-100
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    • 2004
  • An experiment was conducted to establish comparison of ventilation efficiency in an enclosed and conventional growing-finishing pig house. The experimental pigs were in winter and summer. The main results of the experiment are as follows : Then the air from planar slot inlet the pig house flow out through the sidewall outlet operated by exhaust fan(Gl). The second structure has an air input through the circular duct inlet are plated side the juncture of the entering wall and the air into the pig house flow out through the chimney and pit outlet are operated by exhaust fan(G2). Through the air into relay fan the pig house flow out through the curtains in sidewall(G3). Similarly, air comes in through the circular duct inlet are placed the air into the pig house flow out through the curtains in sidewall (G4). Air flow rate on the floor level which is the low part of pen and the living area of pigs in the G2 and G4 system during winter was measured at 0.2 to 0.3 m/s at the 0.5 to 0.6 m/s at the maximum ventilation efficiency. As for the results of detrimental gas(ammonia) concentration ratio analysis, while G2 and G4 system sustained of summer 13.3 $\~$ 16.6 ppm, winter 14.0 $\~$ 14.6 ppm level, Gl and G3 system sustained of summer 14.6 $\~$ 20.3 ppm, winter 20.3 $\~$ 25.0 ppm, and the latter one is lower than that of the G1 and G3 system.

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