• Journal of Microbiology and Biotechnology
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• 제30권11호
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• pp.1688-1696
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• 2020

Soil physical and chemical characteristics, soil potential denitrification rates (PDR), community composition and nirK-, nirS- and nosZ-encoding denitrifiers were studied by using MiSeq sequencing, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment polymorphism (T-RFLP) technologies base on short-term (5-year) tillage field experiment. The experiment included four tillage treatments: conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), no-tillage with crop residue retention (NT), and rotary tillage with crop residue removed as control (RTO). The results indicated that soil organic carbon, total nitrogen and NH₄⁺-N contents were increased with CT, RT and NT treatments. Compared with RTO treatment, the copies number of nirK, nirS and nosZ in paddy soil with CT, RT and NT treatments were significantly increased. The principal coordinate analysis indicated that tillage management and crop residue returning management were the most and the second important factors for the change of denitrifying bacteria community, respectively. Meanwhile, this study indicated that activity and community composition of denitrifiers with CT, RT and NT treatments were increased, compared with RTO treatment. This result showed that nirK, nirS and nosZ-type denitrifiers communities in crop residue applied soil had higher species diversity compared with crop residue removed soil, and denitrifying bacteria community composition were dominated by Gammaproteobacteria, Deltaproteobacteria, and Betaproteobacteria. Therefore, it is a beneficial practice to increase soil PDR level, abundance and community composition of nitrogen-functional soil microorganism by combined application of tillage with crop residue management.

• 한국토양비료학회지
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• 제49권5호
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• pp.548-554
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• 2016

Optimal range of soil physical quality to enhance crop productivity or to improve environmental health is still in dispute for the upland soil. We hypothesized that the optimal range might be established by comparing soil physical parameters and their interactions inhibiting crop growth. The parameter identifying optimal range covered favorable conditions of aeration, permeability and root extension. To establish soil physical standard two experiments were conducted as follows; 1) investigating interactions of bulk density and aeration porosity in the laboratory test and 2) determining effects of soil compaction and deep & conventional tillage on physical properties and crop growth in the field test. The crops were Perilla frutescens, Zea mays L., Solanum tuberosum L. and Secale cereael. The saturated hydraulic conductivity, bulk density from the root depth, root growth and stem length were obtained. Higher bulk density showed lower aeration porosity and hydraulic conductivity, and finer texture had lower threshold bulk density at 10% aeration bulk density. Reduced crop growth by subsoil compaction was higher in silt clay loam compared to other textures. Loam soil had better physical improvement in deep rotary tillage plot. Combined with results of the present studies, the soil physical quality was possibly assessed by bulk density index. Threshold subsoil bulk density as the upper value were $1.55Mg\;m^{-3}$ in sandy loam, $1.50Mg\;m^{-3}$ in loam and $1.45Mg\;m^{-3}$ in silty clay loam for optimal soil physical quality in upland.

• 한국초지조사료학회지
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• 제8권3호
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• pp.92-98
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• 1988

山地초지에 고토와 함유된 有機質複合비료의 施用이 목장의 건물수량, 수량구성요소 및 식생변화에 마치는 影響을 究明코자 비종과 시비수준을 달리한 5處理를 亂塊法 3反復으로 團場配置하여 1984年 9月부터 1986年 10月까지 水原 근교의 신 개척지에서 本 試餘을 수행하였던 바 그 결과를 요약하면 다음과 같다. 1. 목장의 耐冬性, 生育勢 및 被覆率 等 初期生 育狀態는 有機質複함포료 施用區가 단비시용에 比해 良好하였다. 2. 2年평균 목장의 乾物收量은 시비水準間에는 有意差있는 增收했果가 있었으며, 少肥施用水準에서 단비구의 乾物수량 7,930.6kg/ha에 比해 有機質 複合비료 施用區는 8,693.1kg/ha로서10%, 普肥施 用水準에서는 單肥區의 11,122.6kg/ha에 比해 有機 質複合肥料 施用區는 12,758.7kg/ha를 生産하여 15% 회수되었으나 有意性은 없었다. 3. 植牛別 건물수량은 未本科 救草의 경우 施肥量이 많아짐에 따라 有意差있게 증가하였으며 (P<0.01), 荳科救草의 收量은 無肥區는 施肥區(少肥, 普肥)에 比해 顧著히 회수되었으나(P< 0.01), 少肥와 普肥施用水準 問에는 有意性이 없었다. 한편 肥種問에서는 有機質複合肥料 施用區가 單肥에 比해 未本科 목초는 8-14%, 荳科는 26-29% 增收되었으나 有意性은 없었다. 4.. 植生構成比率은 草地管理時에 施肥 하지 않은 곳은 荳科의 優古現象이 나타났으나, 3要素 施用區는 植生比率 및 목초율이 적합하였으며, 유기質複合비료 施用區의 荳科比率은 單肥施用區에 比해 漸次 增加되는 碩向이였다. 5. 試嚴後 士壞의 化學成分은 試餘前에比해 顯 著히 높아졌으나 施肥水準을 平均하여 볼때 有機質 複合nE料 施用區는 單肥에 比해 有機物과 有郊憐嚴含量은 높았고, pH 및 置換性 醒基는 若千 낮은 碩向이었다.

• 한국작물학회지
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• 제51권6호
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• pp.505-512
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• 2006

In Korea, silicate fertilization (SF) is being practiced every four years to enhance rice production. However, the relationship between nitrogen (N) and SF in view of growth characteristics and grain yield of rice has not been examined after watermelon cropping in plastic film house. This study was carried out to identify useful critical N and Si fertilizer levels to sustain grain yield and to improve N use efficiency for rice. The watermelon-rice cropping system has maintained for three seasons in each year from 1998 to 2001 by farmer before this experiment. Experiments on N and Si fertilization levels were evaluated with Hwayoungbyeo (Oryza sativa L.) in 2002 and 2003 at Uiryeong, Korea. The goal of this experiment was to find out the optimum N and Si levels to sustain rice yield by reducing excessive N fertilizer in watermelon-rice cropping system. Nitrogen fertilization (NF) levels were three ($0,\;57,\;114kg\;ha^{-1};0,\;50,\;100%$ of conventional NF amount) and five (0, 25, 50, 75, 100%) in 2002 and 2003, respectively, and combined with three SF levels ($70,\;130,\;180mg\;kg^{-1};100,\;150,\;200%$ which were adjusted with Si fertilizer in soil) were evaluated for the improvement of N and Si fertilization level in both years. Rice yielded 3.98-5.95 and 2.84-4.02 t/ha in 2002 and 2003, respectively. Our results showed the combinations of 50% and 100% of N with 200% level of Si produced the highest grain yield in both years, respectably. The grain yield was greatly improved in plot of N25% level when compared to conventional NF (Nl00%) in 2003. In conclusion, NF amount could be reduced about 50% compared to recommended level by specific fertilization of N and Si combination levels for rice growing and grain yield after cultivation watermelon in paddy field.

• 한국토양비료학회지
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• 제48권6호
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• pp.610-617
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• 2015

The impact of 1 pound of nitrous oxide ($N_2O$) on warming the atmosphere is almost 310 times that of 1 pound of carbon dioxide. Agricultural soil management is the largest source of $N_2O$ emissions, accounting for about 73% of total $N_2O$ emissions. This study was conducted to evaluate the nitrous oxide emission in the cultivation of soybean during the first year of No-tillage (NT) and Conventional-tillage (CT) practices, under the various conditions such as different kinds of fertilizers, soil temperature, and moisture level. In the experiment, we set CT and NT treatments into 4 different groups - control treatments (no fertilization), green manure treatments, chemical fertilizer treatments and organic manure treatments. In the case of chemical fertilizer treatments, $N_2O$ emission of NT treatment was 7.78 to 22.59% lower than CT treatment. In organic manure treatment, $N_2O$ emission of NT treatment was 6.62% higher than CT treatment in August. But In July and September, $N_2O$ emission of NT treatment was 9.50% 28.38% lower than CT treatment, respectively. Soil temperature was correlated with $N_2O$ emission positively. In the future, continued long-term research on influence of various environmental factors on the generation of $N_2O$ and the economic value of no-till farming is required.

• 한국유기농업학회지
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• 제26권4호
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• pp.731-743
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• 2018

배추 유기재배 시 녹비작물과 추비방법이 배추의 생육과 질소이용에 미치는 영향을 구명하기 위하여 2012년부터 2014년까지 수원에 있는 국립농업과학원 포장에서 시험을 수행하였다. 녹비작물은 여름철 크로타라리아와 겨울철 헤어리베치를 이용하였다. 녹비처리 방법은 전면경운 토양혼입방법과 부분경운 토양혼입방법, 그리고 무경운 예취피복처리를 비교하였다. 추비방법은 녹비만 처리한 것과 녹비 처리 후 유박과 액비로 추비한 처리를 비교 하였다. 시험결과 녹비 처리 방법은 예취 후에 경운하여 토양혼입 하는 것보다 무경운 피복처리 하는 것이 좋았다. 봄배추재배 시 동계 녹비작물 헤어리베치 재배로 정상적인 생육과 수량이 가능하였고 관행의 화학비료 처리구에 비하여 무름병과 Tip-burn 발생이 적어 수량성도 높았다. 가을재배에서는 녹비 연속재배와 유박 추비로 관행의 화학비료처리구와 비슷한 수량을 보였다. 하지만 하계 단기성 녹비작물 재배 시에는 관행 수량의 70% 내외에 그쳤다. 녹비재배에 이은 액비 활용 양분관리로 관행과 비슷한 수량이 가능하였고 토양중 무기태 질소가 안정적으로 유지되고 질소이용효율도 향상되었다. 결론적으로 배추 유기재배를 위해서는 겨울과 여름 녹비작물을 재배하고 가을 작기를 선택하여 액비로 추비하는 것이 권장된다.

• 한국토양비료학회지
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• 제46권6호
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• pp.652-658
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• 2013

A huge number of greenhouse soils in Korea have accumulated mineral elements which induce many nutritional and pathological problems. The present study was performed to the effects of the reduced fertilization on plant growth, and uptake and partitioning of minerals (N, P, K) and soluble carbohydrates using highly minerals-accumulated farmer's greenhouse soil. On the basis of the recommended application for tomato crop, the application rates of N, P and K were 110(50%)-5.2(5%)-41.5(35%)kg $ha^{-1}$, respectively, using Hoagland's nutrient solution. Tomato growth rates during the whole experiment were not significant between treatments, but it was found that a decrease in daily growth represented after 60 days of treatment (DAT). The reduced application led to a drastic decrease in the concentration of N, P and K in fruits, and, thus, this resulted in lower uptake after 40 DAT. The lower phloem export and utilization of soluble carbohydrates caused an accumulation of extra-carbohydrates in leaves, stems and fruits in the reduced application. The reduced fertilization induced the capture of N, P and K in leaves and of soluble carbohydrates in stems compared to the conventional application. In this study, we suggest that it is possible to delay the first fertigation time in minerals-accumulated soils without an adverse impact on crop growth, but it is necessary to regularly monitor mineral status in soil to ensure a balanced uptake, synthesis and partitioning of minerals and carbohydrates.

• Journal of Biosystems Engineering
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• 제27권3호
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• pp.259-266
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• 2002

It is crucial to know spatial soil variability for precision farming. However, it is time-consuming, and difficult to measure spatial soil properties. Therefore, there are needs fur sensing technology to estimate spatial soil variability, and for electronic mapping technology to store, manipulate and process the sampled data. This research was conducted to develop a real-time soil organic matter sensor and an electronic mapping system. A soil organic matter sensor was developed with a spectrophotometer in the 900∼1,700 nm range. It was designed in a penetrator type to measure reflectance of soil at 15cm depth. The signal was calibrated with organic matter content (OMC) of the soil which was sampled in the field. The OMC was measured by the Walkeley-Black method. The soil OMCs were ranged from 0.07 to 7.96%. Statistical partial least square and principle component regression analyses were used as calibration methods. Coefficient of determination, standard error prediction and bias were 0.85 0.72 and -0.13, respectively. The electronic mapping system was consisted of the soil OMC sensor, a DGPS, a database and a makeshift vehicle. An algorithm was developed to acquire data on sampling position and its OMC and to store the data in the database. Fifty samples in fields were taken to make an N-fertilizer dosage map. Mean absolute error of these data was 0.59. The Kring method was used to interpolate data between sampling nodes. The interpolated data was used to make a soil OMC map. Also an N-fertilizer dosage map was drawn using the soil OMC map. The N-fertilizer dosage was determined by the fertilizing equation recommended by National Institute of Agricultural Science and Technology in Korea. Use of the N-fertilizer dosage map would increase precision fertilization up to 91% compared with conventional fertilization. Therefore, the developed electronic mapping system was feasible to not only precision determination of N-fertilizer dosage, but also reduction of environmental pollution.

• 한국작물학회지
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• 제39권4호
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• pp.373-381
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• 1994

호밀을 답리작으로 재배시 수량, 일반사료성분과 에너지 함량 및 수량을 질소시비량 및 예취시기에 따른 반응을 요약하면 다음과 같다. 1. 호밀은 질소시비량이 30kg/10a까지 많을수록 청예 및 건물수량이 증가하고 건물률은 감소하였으며 식물체의 엽신과 엽초의 비율은 증가하는 반면 줄기와 이삭의 비율은 감소하였다. 2. 호밀은 질소시비량이 많을수록 단백질, 총가소화양분(TDN), 무기물 및 에너지 함량과 수량이 모두 증가하였으며 ADF(acid detergent fiber) 와 NDF(neutral detergent fiber)의 함량은 감소하고 상대적사료가치가 높아졌다. 3. 질소시비량이 많아지면 예취시기가 늦더라도 전체 조단백질 함량 중에서 가급태 단백질이 차지하는 비율이 크게 낮아지지 않았다. 4. 호밀의 수확적기는 관행사료가치의 관점에서 는 대체로 유숙기 무렵으로 판단되었으나 사료의 에너지 관점에서는 이와 다소 차이가 있었다. 증체에너지(NEG)와 유지에너지(NEM) 면에서 본 수확적기는 개화후기에 높고 관행사료가치보다 10일정도 빨랐으며 에너지추정값(ENE)과 필유에너지(NEL)는 유숙기 무렵으로 같았다.

• 한국토양비료학회지
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• 제45권6호
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• pp.1143-1152
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• 2012

본 연구진은 2007년 농진청과 통계청에서 수집한 농축산물소득자료 통계 값으로 국가평균 탄소성적을 산정하는 top-down 방식의 자료수집 방법을 구축하였다. 또한, 본 연구진은 2011년 전북 군산 지역 관행농 쌀 생산 농가 중 벼 평균 재배면적이 3.3 ha인 네 곳의 대규모 생산농가를 섭외하여 면접조사로 사례를 분석하는 bottom-up 방식의 전과정 목록 (LCI, life cycle Inventory)도 구축하였다. 본 연구는 관행농 쌀 생산체계에 대한 전과정 평가를 국립농업과학원에서 구축한 top-down 방식과 사례분석을 통한 bottom-up 방식으로 수행한 결과를 비교하기 위하여 수행되었다. 전과정 목록분석 결과 $CO_2$은 무기질 비료 생산과 벼 재배과정에서 배출량이 가장 많았고, $CH_4$와 $N_2O$은 대부분 벼 재배과정에서 배출되었다. 관행농 쌀 1 kg 생산을 기준으로 하는 탄소성적은 국가평균값이 2.39E+00 kg $CO_2$-eq. $kg^{-1}$, 사례분석이 1.04E+00 kg $CO_2$-eq. $kg^{-1}$으로 국가평균 탄소성적이 사례분석보다 높았다. 쌀 생산 전과정에 투입되는 농자재량은 국가평균과 사례분석이 유사하거나 오히려 사례분석이 더 높게 나타났으나 작기 당 수확량이 높아 사례분석의 탄소성적이 유리한 결과가 나왔다. 관행농 쌀 생산체계에서 각 생산공정별 환경영향을 분석한 결과 화학비료 생산단계가 대부분의 환경영향범주에서 기여도가 가장 높게 나타났으나, GWP 범주는 벼 재배에 의한 환경영향 기여도가 가장 컸다. 관행농 쌀 생산에서 탄소성적을 결정하는 주요 요인은 논토양에서 발생하는 메탄가스와 비료투입량 및 벼 수확량이었다. 본 연구 결과는 향후 '농산물 저탄소인증' 시범사업에서 배출량 산정을 위한 기초자료와 벼논에서 온실가스를 줄이기 위한 영농법 개발에 활용될 것으로 기대된다. '저탄소 농산물 인증제도' 시범사업의 성공을 위해서 쌀을 포함한 농작물에 대한 실제 농가를 대상으로 하는 사례분석 연구가 더욱 늘어나야 할 것이다. 현 단계에서는 자발적인 참여 농가를 대상으로 하여 활동 데이터 수집을 늘리고, 자료의 일관성과 대표성 보완을 위하여 농가의 활동데이터를 수집할 때 모집단 선발과 수집기간 등에 대한 논의가 필요하다. 또한 현재 국립농업과학원에서 구축하고 있는 DB는 2007년 소득자료를 기준으로 하므로 인증사업 시행시 사례분석을 적용할 때 몇 가지 한계와 보완사항이 요구되었다. 첫째, 국가평균 통계와 실제 대상농가 간 품종 및 생산연도에 의한 생산량 차이를 보완하기 위한 가중치 적용 등이 필요할 것으로 판단되었다. 둘째, 현재 국가평균 DB를 기준으로 설정된 시스템 경계에서 육묘 용 상토와 볏짚 및 쌀겨 등 부산물과 수확 후 도정 및 포장 등에 대한 시스템경계 확장 연구 및 이에 관한 LCI DB 구축이 요구되었다.