• Journal of Embryo Transfer
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• v.29 no.3
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• pp.207-219
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• 2014

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in $2.5{\times}10^5cells/ml$ and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of $IL-1{\beta}$ (0.1, 1, 10 and 100 ng/ml) were higher than without $IL-1{\beta}$, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

• Journal of Microbiology and Biotechnology
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• v.12 no.6
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• pp.878-881
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• 2002

The goal of the current research was to assess the influence of the growth rate of Nocardia amarae on its overall metal binding capacity. Batch sorption isotherms for cadmium (Cd), copper (Cu), and nickel (Ni) showed that Nocardia cells harvested from chemostat cultures at a dilution rate of $0.33d^-1$ had a significantly higher metal sorption capacity than cells grown at 0.5 and $1d^-1$. The cell surface area estimated using a dye technique indicated that pure N. amarae cells grown at a lower growth rate had a significantly more specific surface area than cells harvested from a higher growth rate operation. Accordingly, this difference in the specific surface area seemed to indicate that the higher metal sorption capacity of the slowly growing Nocardia cells was due to their higher specific surface area.

• Microbiology and Biotechnology Letters
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• v.48 no.4
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• pp.423-428
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• 2020

In vitro evolution is a powerful technique for the engineering of yeast strains to study cellular mechanisms associated with evolutionary adaptation; strains with desirable traits for industrial processes can also be generated. There are two distinct approaches to generate evolved strains in vitro: the sequential transfer of cells in the stationary phase into fresh medium or the continuous growth of cells in a chemostat bioreactor via the constant supply of fresh medium. In culture, evolutionary forces drive diverse adaptive mechanisms within the cell to overcome environmental or intracellular stressors. Especially, this engineering strategy has expanded to the field of human cell lines; the understanding of such adaptive mechanisms provides promising targets for the treatment of human genetic diseases and cancer. Therefore, this technology has the potential to generate numerous industrial, medical, and academic applications.

• The Journal of the Convergence on Culture Technology
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• v.7 no.1
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• pp.588-594
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• 2021

Due to the recent proliferation of video contents, previous contents expressed as the character or the picture are being replaced to video and growth of video contents is being boosted because of emerging new platforms. As this accelerated growth has a great impact on the process of universalization of technology for ordinary people, video production and editing technologies that were classified as expert's areas can be easily accessed and used from ordinary people. Due to the development of these technologies, tasks like that recording and adjusting that depends on human's manual involvement could be automated through object tracking technology. Also, the process for situating the object in the center of the screen after finding the object to record could have been automated. Because the task of setting the object to be tracked is still remaining as human's responsibility, the delay or mistake can be made in the process of setting the object which has to be tracked through a human. Therefore, we propose a novel object tracking technique of CMT combining the face detection technique utilizing Haar cascade classifier. The proposed system can be applied to an effective and robust image tracking system for continuous object tracking on the smartphone in real time.

• Journal of Microbiology
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• v.44 no.2
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• pp.155-161
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• 2006

Comparative analysis of microbial communities in a sequencing batch reactor which performed enhanced biological phosphorus removal (EBPR) was carried out using a cultivation-based technique and 16S rRNA gene clone libraries. A standard PCR protocol and a modified PCR protocol with low PCR cycle was applied to the two clone libraries of the 16S rRNA gene sequences obtained from EBPR sludge, respectively, and the resulting 424 clones were analyzed using restriction fragment length polymorphisms (RFLPs) on 16S rRNA gene inserts. Comparison of two clone libraries showed that the modified PCR protocol decreased the incidence of distinct fragment patterns from about 63 % (137 of 217) in the standard PCR method to about 34 % (70 of 207) under the modified protocol, suggesting that just a low level of PCR cycling (5 cycles after 15 cycles) can significantly reduce the formation of chimeric DNA in the final PCR products. Phylogenetic analysis of 81 groups with distinct RFLP patterns that were obtained using the modified PCR method revealed that the clones were affiliated with at least 11 phyla or classes of the domain Bacteria. However, the analyses of 327 colonies, which were grouped into just 41 distinct types by RFLP analysis, showed that they could be classified into five major bacterial lineages: ${\alpha},\;{\beta},\;{\gamma}-$ Proteobacteria, Actinobacteria, and the phylum Bacteroidetes, which indicated that the microbial community yielded from the cultivation-based method was still much simpler than that yielded from the PCR-based molecular method. In this study, the discrepancy observed between the communities obtained from PCR-based and cultivation-based methods seems to result from low culturabilities of bacteria or PCR bias even though modified culture and PCR methods were used. Therefore, continuous development of PCR protocol and cultivation techniques is needed to reduce this discrepancy.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.22 no.2
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• pp.105-113
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• 2012

Maintenance training is generally provided to teach employees new knowledge and techniques in order to increase their qualities. It means that the purpose of maintenance training is to increase an employee's knowledge or technique level and to maintain or increase their performance level through continuous training in their field, and the methods, contents and the level of training vary depending on the type of job they perform. Maintenance training is more important for jobs that require continuous technical increases or research, or for professional jobs that continuously require new knowledge and techniques. The purpose of this study is to provide quality service to consumers by responding to the rapidly changing jewelry distribution environment and to quickly and accurately acquire new and advanced jewelry inspection and appraisal techniques, and to contribute to the healthy consumption culture through a general quality increase in the jewelry industry.

• Journal of the Korea Society of Computer and Information
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• v.10 no.4 s.36
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• pp.173-179
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• 2005

The continuous development of computing technique and network technology bring the explosive growth of the Internet, it accomplished the role which is import changes the base facility in the social whole and public infra, industrial infrastructure, culture on society-wide to Internet based environment. Recently the rapid development of information and technology environment is quick repeated the growth and a development which is really unexampled in the history but it has a be latent vulnerability, Therefore the damage from this vulnerability like worm, hacking increases continually. In this paper, in order to resolve this problem, implement the analysis system for harmful traffic for defending new types of attack and analyzing the traffic takes a real-time action against intrusion and harmful information packet.

• International conference on construction engineering and project management
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• 2011.02a
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• pp.335-339
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• 2011

The unique characteristics of the construction industry - such as the fragmentation of its processes, varied scope of works and diversity of its participants - are contributory factors to poor project performance. Several issues are unresolved due to the lack of a comprehensive technique to measure project outcomes including: inefficient decision making, insufficient communication, uncertain site conditions, a continuously changing environment, inharmonious working relationships, mismatched objectives within the project team and a blame culture. One approach to overcoming these problems appears to be to measure performance by gauging contractor satisfaction (Co-S) levels, but this has not been widely investigated as yet. Additionally, the key Co-S dimensions at the project level are still not fully identified. This paper concerns a study of satisfaction dimensions, primarily by a postal questionnaire survey of construction contractors registered by the Malaysian Construction Industry Development Board (CIDB). Eight satisfaction dimensions are identified that are significantly and substantially relate to these contractors - comprising: project cost performance, schedule performance, product performance, design satisfaction, site safety, project profitability, business performance and relationships between participants. -Each of these dimensions is accorded different priority levels of satisfaction by different contractors. The output of this study will be useful in raising the awareness and understanding of project teams regarding contractors' needs, mutual objectives and open communication to help to deliver a successful project.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.487-492
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• 2022

Universities are looking for various methods to enhance educational competence level suitable for the rapidly changing social environment. This study suggests a method to promote academic and educational achievements by reducing drop-out rate from their majors through implementation of pre-survey of satisfaction that revised and complemented survey items. To supplement the CQI method implemented after a general satisfaction survey, a pre-survey of satisfaction was carried out. To consolidate students' competences, this study made prediction and analysis of data with more importance possible using the Random Forest of the machine learning technique that can be applied to AI Medici platform, whose design is underway. By pre-processing the pre-survey of satisfaction, the students information enrolled in classes were defined as an explanatory variable, and they were classified, and a model was created and learning was conducted. For the experimental environment, the algorithms and sklearn library related in Jupyter notebook 3.7.7, Python 3.7 were used together. This study carried out a comparative analysis of change in educational satisfaction survey, carried out after classes, and trends in the drop-out students by reflecting the results of the suggested method in the classes.

• KSBB Journal
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• v.13 no.1
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• pp.83-89
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• 1998

Using a cellulose macroporous microcarrier, HeLa cells were cultivated in 100mL spinner flask(Bellco Co., USA) and confluent cell laden microcarriers were subcultured by bead-to-bead cell transfer method. In macroporous mcirocarrier-HeLa system viable suspended cells played an important role in bead-to-bead cell transfer and that could be increased by use of RPMI-1640, a calcium-ion-reduced-media and high speed agitation. Successful bead-to-bead cell transfers were performed continuously three time in spinner flask. We applied this technique to produce recombinant Vaccinia virus which express $\beta$-galactosidase. Recombinant protein yield of bead-to-bead transferred culture was comparable to conventional microcarrier cultures that were inoculated by cells detached from T-flask. Although trypsinization is a useful method for subculturing microcarriers in some cases, that process adds quality control problem and handling steps to large scale cell production. There fore, bead-to-bead cell transfer technique offers another convenient and efficient scale-up method for continuous microcarrier cultures.