• Title/Summary/Keyword: contamination probability

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Risk assessment of vibriosis by Vibrio cholerae and Vibrio vulnificus in whip-arm octopus consumption in South Korea

  • Oh, Hyemin;Yoon, Yohan;Ha, Jimyeong;Lee, Jeeyeon;Shin, Il-Shik;Kim, Young-Mog;Park, Kwon-Sam;Kim, Sejeong
    • Fisheries and Aquatic Sciences
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    • v.24 no.6
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    • pp.207-218
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    • 2021
  • This study evaluated the risk of foodborne illness from highly pathogenic Vibrio spp. (Vibrio vulnificus and V. cholerae) by raw whip-arm octopus (Octopus minor) consumption. In total 180 samples of raw whip-arm octopus were collected from markets and examined for the prevalence of V. vulnificus and V. cholerae. Predictive models describing the kinetic behavior of Vibrio spp. in raw whip-arm octopus were developed, and the data on amounts and frequency of raw whip-arm octopus consumption were collected. Using the collected data, a risk assessment simulation was conducted to estimate the probability of foodborne illness raw whip-arm octopus consumption using @RISK. Initial contamination levels of Vibrio spp. in raw whip-arm octopus were -3.9 Log colony-forming unit/g, as estimated by beta distribution fitting. The developed predictive models were appropriate to describe Vibrio spp. in raw whip-arm octopus during distribution and storage with R2 values of 0.946-0.964. The consumption frequency and daily consumption amounts of raw whip-arm octopus per person were 0.47% and 57.65 g, respectively. The probability of foodborne illness from raw whip-arm octopus consumption was estimated to be 8.71 × 10-15 for V. vulnificus and 7.08 × 10-13 for V. cholerae. These results suggest that the risk of Vibrio spp. infection from raw whip-arm octopus consumption is low in South Korea.

Quantitative microbial risk assessment indicates very low risk for Vibrio parahaemolyticus foodborne illness from Jeotgal in South Korea

  • Choi, Yukyung;Kang, Joohyun;Lee, Yewon;Seo, Yeongeun;Kim, Sejeong;Ha, Jimyeong;Oh, Hyemin;Kim, Yujin;Park, Eunyoung;Lee, Heeyoung;Lee, Soomin;Rhee, Min Suk;Yoon, Yohan
    • Fisheries and Aquatic Sciences
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    • v.25 no.9
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    • pp.463-472
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    • 2022
  • In this study, a microbial risk assessment was performed for the bacteria Vibrio parahaemolyticus, which causes a foodborne illness following the consumption of Jeotgal, a fermented seafood in South Korea. The assessment comprised of six stages: product, market, home, consumption, dose-response, and risk. The initial contamination level (IC) was calculated based on the prevalence of V. parahaemolyticus in 90 Jeotgal samples. The kinetic behavior of V. parahaemolyticus was described using predictive models. The data on transportation conditions from manufacturer to market and home were collected through personal communication and from previous studies. Data for the Jeotgal consumption status were obtained, and an appropriate probability distribution was established. The simulation models responding to the scenario were analyzed using the @RISK program. The IC of V. parahaemolyticus was estimated using beta distribution [Beta (1, 91)]. The cell counts during transportation were estimated using Weibull and polynomial models [δ = 1 / (0.0718 - 0.0097 × T + 0.0005 × T2)], while the probability distributions for time and temperature were estimated using Pert, Weibull, Uniform, and LogLogistic distributions. Daily average consumption amounts were assessed using the Pareto distribution [0.60284,1.32,Risk Truncate(0,155)]. The results indicated that the risk of V. parahaemolyticus infection through Jeotgal consumption is low in South Korea.

Quantitative risk assessment of foodborne Salmonella illness by estimating cooking effect on eggs from retail markets

  • Hyemin Oh;Yohan Yoon;Jang Won Yoon;Se-Wook Oh;Soomin Lee;Heeyoung Lee
    • Journal of Animal Science and Technology
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    • v.65 no.5
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    • pp.1024-1039
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    • 2023
  • In this study, we performed a quantitative microbial risk assessment (QMRA) of Salmonella through intake of egg consumption after cooking (dry-heat, moist-heat, and raw consumption). Egg samples (n = 201) from retail markets were analyzed for the presence of Salmonella. In addition, temperature and time were investigated during egg transit, storage, and display. A predictive model was developed to characterize the kinetic behavior of Salmonella in eggs, and data on egg consumption and frequency were collected. Eventually, the data was simulated to estimate egg-related foodborne illnesses. Salmonella was not found in any of the 201 egg samples. Thus, the estimated initial contamination level was -4.0 Log CFU/g. With R2 values of 0.898 and 0.922, the constructed predictive models were adequate for describing the fate of Salmonella in eggs throughout distribution and storage. Eggs were consumed raw (1.5%, 39.2 g), dry-heated (57.5%, 43.0 g), and moist-heated (41%, 36.1 g). The probability of foodborne Salmonella illness from the consumption of cooked eggs was evaluated to be 6.8×10-10. Additionally, the probability of foodborne illness not applied cooking methods was 1.9×10-7, indicating that Salmonella can be reduced by cooking. Therefore, the risk of Salmonella infection through consumption of eggs after cooking might be low in S. Korea.

Bacterial Contamination Reduction of Minimally Processed Agricultural Products using Antibacterial Foods and Molecular Biological Analysis (항균성 식품을 이용한 간편섭취 농산물 미생물오염의 감소 및 분자생물학적 분석)

  • Kim, Jin-Ah;Lee, Sung-Deuk;Hwang, Kwang-Ho;Song, Mi-Ok;Park, Jung-Eun;Kim, Da-Mi;Chung, Ae-Hee;Oh, Young-Hee
    • Journal of Food Hygiene and Safety
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    • v.31 no.1
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    • pp.8-14
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    • 2016
  • The purposes of this study are to confirm ways to reduce bacteria of minimally processed agricultural products, using antibacterial foods that are easily available in home and to improve bacterial hygienic condition of them. We chose garlic-allicin, ginger-gingerol, green tea-catechin, cinnamon-cinnamic aldehyde, wasabi-allyl isothiocyanate as antibacterial foods and their unique antibiotic materials. We confirmed the better washing effect when these antibiotic extracts were used, compared to washing effect by only distilled water. Their antibiosis was proved by statistical processing. PFGE (Pulsed Field Gel Electrophoresis) of Bacillus cereus shows continuous contamination probability of minimally processed vegetables by same product suppliers and the necessity of systematic measures against bacterial contamination.

Infection Risks Faced by Public Health Laboratory Services Teams When Handling Specimens Associated With Coronavirus Disease 2019 (COVID-19)

  • Wong, Chun-Kwan;Tsang, Dominic N.C.;Chan, Rickjason C.W.;Lam, Edman T.K.;Jong, Kwok-Kwan
    • Safety and Health at Work
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    • v.11 no.3
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    • pp.372-377
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    • 2020
  • Infection risks of handling specimens associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by public health laboratory services teams were assessed to scrutinize the potential hazards arising from the work procedures. Through risk assessments of all work sequences, laboratory equipment, and workplace environments, no aerosol-generating procedures could be identified except the procedures (mixing and transfer steps) inside biological safety cabinets. Appropriate personal protective equipment (PPE) such as surgical masks, protective gowns, face shields/safety goggles, and disposable gloves, together with pertinent safety training, was provided for laboratory work. Proper disinfection and good hand hygiene practices could minimize the probability of SARS-CoV-2 infection at work. All residual risk levels of the potential hazards identified were within the acceptable level. Contamination by gloved hands was considered as a major exposure route for SARS-CoV-2 when compared with eye protection equipment. Competence in proper donning and doffing of PPE accompanied by hand washing techniques was of utmost importance for infection control.

An Experimental Study on the Characteristics of Direct Photoelectric Charging (직접 광대전의 대전특성에 관한 실험적 연구)

  • Lee, Chang-Sun;Kim, Yong-Jin;Kim, Sang-Sao
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.24 no.6
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    • pp.753-759
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    • 2000
  • Photoelectric charging is a very efficient way of charging small particles. This method can be applied to combustion measurement, electrostatic precipitator, metal separation and control of micro-contamination. To understand the photoelectric charging mechanism, particle charging of silver by exposure to ultraviolet is investigated in this study. Average charges and charge distributions are measured at various conditions, using two differential mobility analyzers, a condensation nucleus counter, and an aerosol electrometer. The silver particles are generated in a spark discharge aerosol generator. After that process, the generated particles are charged in the photoelectric charger using low-pressure mercury lamp that emits ultraviolet having wavelength 253.7 nm. The results show that ultra-fine particles are highly charged by the photoelectric charging. The average charges linearly increase with increasing particle size and the charge distribution change with particle size. These results are discussed by comparison with previous experiments and proposed equations. It is assumed that the coefficient of electron emission probability is affected by initial charge. The results also show that the charge distribution of a particle is dependent on initial charge. Single changed particle, uncharged particle and neutralized particle are compared. The differences of charge distribution in each case increase with increasing particle size.

Robust Image Hashing for Tamper Detection Using Non-Negative Matrix Factorization

  • Tang, Zhenjun;Wang, Shuozhong;Zhang, Xinpeng;Wei, Weimin;Su, Shengjun
    • Journal of Ubiquitous Convergence Technology
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    • v.2 no.1
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    • pp.18-26
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    • 2008
  • The invariance relation existing in the non-negative matrix factorization (NMF) is used for constructing robust image hashes in this work. The image is first re-scaled to a fixed size. Low-pass filtering is performed on the luminance component of the re-sized image to produce a normalized matrix. Entries in the normalized matrix are pseudo-randomly re-arranged under the control of a secret key to generate a secondary image. Non-negative matrix factorization is then performed on the secondary image. As the relation between most pairs of adjacent entries in the NMF's coefficient matrix is basically invariant to ordinary image processing, a coarse quantization scheme is devised to compress the extracted features contained in the coefficient matrix. The obtained binary elements are used to form the image hash after being scrambled based on another key. Similarity between hashes is measured by the Hamming distance. Experimental results show that the proposed scheme is robust against perceptually acceptable modifications to the image such as Gaussian filtering, moderate noise contamination, JPEG compression, re-scaling, and watermark embedding. Hashes of different images have very low collision probability. Tampering to local image areas can be detected by comparing the Hamming distance with a predetermined threshold, indicating the usefulness of the technique in digital forensics.

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Microbiological Risk Assessment for Milk and Dairy Products in Korea (우유 및 유제품의 안전성 평가를 위한 미생물학적 위해요소의 위해평가)

  • Kim, Hyoun-Wook;Han, Gi-Sung;Park, Beom-Young;Jeong, Seok-Geun;Kim, Hyeon-Shup;Oh, Mi-Hwa
    • Journal of Dairy Science and Biotechnology
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    • v.29 no.2
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    • pp.69-73
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    • 2011
  • Food borne pathogens are a growing concern for human health and food safety throughout the world. Milk and dairy products are commonly associated with spoilage or contamination from a wide variety of physical, microbial, and chemical hazardous. Microbiological risk analysis consists of three components: risk assessment, risk management, and risk communication, and overall objective of this process is ultimately public health protection. The microbiological risk assessment is useful tool to evaluate food safety as it is based on a scientific approach. In addition risk assessment process includes quantitative estimation of the probability of occurrence of microbial hazards to evaluate more accurate human exposure. The aim of this study is to review the microbiological risk assessment on the prevalence of bacterial foodborne pathogens in milk and dairy products.

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A Study on Insulation Performance Enhancement of Existing Transmission Line Considering Overvoltage Analysis Result and Fault Characteristic (과전압 분석 결과와 고장특성을 고려한 기설 송전선로의 절연보강 방법에 관한 연구)

  • Kwak, Joo-Sik;Woo, Jung-Woog;Koo, Kyo-Sun;Kim, Kyung-Tak;Kweon, Dong-Jin
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.23 no.12
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    • pp.162-168
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    • 2009
  • This paper describes a study that is to redesign insulation of transmission lines and determine required the numbers of insulators in order to enhance the insulation performance of existing transmission lines which were constructed with standard insulation design, considering overvoltage analysis results, probabilities and characteristics of faults. To ensure proper insulation distance without deteriorating the required performance, EMTP model is established to calculate maximum overvoltage in the line. The fault records and predicted outage rates due to lightning and contamination in the line were investigated and analyzed respectively. It presents a method to determine the numbers of insulators considering the probability and the characteristic of fault.

Quantitative Risk Assessment of Listeria monocytogenes Foodborne Illness Caused by Consumption of Cheese (위해평가를 통한 치즈에서의 Listeria monocytogenes 식중독 발생 가능성 분석)

  • Ha, Jimyeong;Lee, Jeeyeon
    • Journal of Food Hygiene and Safety
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    • v.35 no.6
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    • pp.552-560
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    • 2020
  • Listeria monocytogenes is a highly pathogenic gram-positive bacterium that is easily isolated from cheese, meat, processed meat products, and smoked salmon. A zero-tolerance (n=5, c=0, m=0/25 g) criteria has been applied for L. monocytogenes in cheese meaning that L. monocytogenes must not be detected in any 25 g of samples. However, there was a lack of scientific information behind this criteria. Therefore, in this study, we conducted a risk assessment based on literature reviews to provide scientific information supporting the baseline and to raise public awareness of L. monocytogenes foodborne illness. Quantitative risk assessment of L. monocytogenes for cheese was conducted using the following steps: exposure assessment, hazard characterization, and risk characterization. As a result, the initial contamination level of L. monocytogenes was -4.0 Log CFU/g in cheese. The consumption frequency of cheese was 11.8%, and the appropriate probability distribution for amount of cheese consumed was a Lognormal distribution with an average of 32.5 g. In conclusion, the mean of probabilities of foodborne illness caused by the consumption of cheese was 5.09×10-7 in the healthy population and 4.32×10-6 in the susceptible population. Consumption frequency has the biggest effect on the probability of foodborne illness, but storage and transportation times have also been found to affect the probability of foodborne illness; thus, management of the distribution environment should be considered important. Through this risk assessment, scientific data to support the criteria for L. monocytogenes in cheese could be obtained. In addition, we recommend that further risk assessment studies of L. monocytogenes in various foods be conducted in the future.