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Manufacture of Gouda Cheese from the Concentrated Milk by the Use of Ultrafiltration (Ultrafiltration을 이용한 Gouda Cheese의 제조)

  • Lee, Yong-Lim;Kim, Sang-Pil;Park, Hee-Kyung;Heo, Tae-Ryeon
    • Microbiology and Biotechnology Letters
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    • v.22 no.1
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    • pp.99-105
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    • 1994
  • In this study we compared traditional cheesemaking process with the process utilizing ultrafiltration(UF) system. The whole milk retentates were prepared by ultrafiltration to volume concentration ratio(VCR) of 2.00:1, 2.25:1 and 2.50:1. Along with the untreated whole milk, there were studies in terms of the change of pH, titratable acidity and Soxhlet-Henkel($\circ $SH) value by mesophilic lactic starter and curd formation by rennet during Gouda cheese manufacture. Due to the increase of buffering effect titratable acidity and $\circ $SH value increased with the higher concentration ratio. When inoculated with the same volume of mesophilic lactic starter, less pH change occurred in UF retentates than in control milk. When added 0.0025% rennet, UF retentates coagulated 16~ 17 minutes ealier then the control milk. Gouda cheese yield from raw milk and UF retentates was 12.5~13.1% equally, but yield efficiency of UF retentate cheese was slightly higher than that of the raw milk cheese. Quantity of whey from retentate cheese was inversely related to VCR. But whey from retentate cheese contained higher percentage of amjor components than that from control milk cheese. In early ripening, the concentrations of lactose and soluble nitrogen compound were higher in retentate cheeses. Lactose content of control milk cheese was 3.49% and that of 2.00:1. 2.25:1, 2.50:1 VCR retentate was 3.77%, 4.89%, 7.03%, respectively. Thus, the more concentrated cheese contained a higher amount of lactose and all the lactose was hyerolyzed durion 35-day ripenion period. Soluble nitrogen compound of control milk cheese was 1.22% and that of UF cheeses was 1.82~2.06%. After 20-day ripening, soluble nitrogen compound increased starply in UF cheese.

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Identification of Wild Yeast Strains and Analysis of Their ${\beta}$-Glucan and Glutathione Levels for Use in Makgeolli Brewing

  • Kang, Sun Hee;Kim, Hye Ryun;Kim, Jae Ho;Ahn, Byung Hak;Kim, Tae Wan;Lee, Jang-Eun
    • Mycobiology
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    • v.42 no.4
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    • pp.361-367
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    • 2014
  • Makgeolli, also known as Takju, is a non-filtered traditional Korean alcoholic beverage that contains various floating matter, including yeast cells, which contributes to its high physiological functionality. In the present study, we assessed the levels of ${\beta}$-glucan and glutathione in various yeast strains isolated from traditional Korean Nuruk and selected a ${\beta}$-glucan- and glutathione-rich yeast strain to add value to Makgeolli by enhancing its physiological functionality through increased levels of these compounds. Yeast ${\beta}$-glucan levels ranged from 6.26% to 32.69% (dry basis) and were strongly species-dependent. Dried Saccharomyces cerevisiae isolated from Nuruk contained $25.53{\mu}g/mg$ glutathione, $0.70{\mu}g/mg$ oxidized glutathione, and $11.69{\mu}g/g$ and $47.85{\mu}g/g$ spermidine and L-ornithine monohydrochloride, respectively. To produce functional Makgeolli, a ${\beta}$-glucan- and glutathione-rich yeast strain was selected in a screening analysis. Makgeolli fermented with the selected yeast strain contained higher ${\beta}$-glucan and glutathione levels than commercial Makgeolli. Using the selected yeast strain to produce Makgeolli with high ${\beta}$-glucan and glutathione content may enable the production of functional Makgeolli.

The drivers and energetics of ionized gas outflows in powerful Type 2 AGN in the local Universe

  • Karouzos, Marios;Woo, Jong-Hak;Bae, Hyun-Jin
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.1
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    • pp.37.2-37.2
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    • 2016
  • There exist scaling relations that link the mass of supermassive black holes with both the velocity dispersion and the mass of the central stellar cusp of their host galaxies. This implies that galaxies co-evolve with their central black holes, potentially through the feedback from actively accreting supermassive black holes (AGN). We use integral field spectroscopy data from the 8.2m Gemini-North telescope to investigate ionized gas outflows in luminous local (z<0.1) Type 2 AGN. Our sample of 6 galaxies was selected based on their [OIII] dust-corrected luminosity (>$10^{42}erg/s$) and signatures of outflows in the [OIII] line profile of their SDSS spectra. These are arguably the best candidates to explore AGN feedback in action since they are < 1% of a large local type 2 AGN SDSS sample selected based on their [OIII] kinematics. Expanding on previously reported results concerning the kinematic decomposition and size determination of these outflows, here we report their photoionization properties and energetics. We find strong evidence that connect the extreme kinematics of the ionized gas with AGN photoionization. The kinematic component related to the AGN-driven outflow is clearly separated from other kinematic components, such as gravitation- or stellar-driven motions, on the velocity and velocity dispersion diagram. Our spatially resolved kinematic analysis reveals that up to 90% of the mass and kinetic energy of the outflow is contained within the central kiloparcec of the galaxy. The total mass and kinetic energy of the outflow correlate well with the AGN bolometric luminosity, resulting in energy conversion efficiencies between 0.01% and 1%. Intriguingly, we detect ubiquitous signs of ongoing circumnuclear star formation. Their small size, the centrally contained mass and energy, and the universally detected circumnuclear star formation cast doubts on the potency of these AGN-driven outflows as agents of negative feedback.

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Depositional Characteristics of Atmospheric PBDEs on Pine Needles, Bark and Soil (대기 중 폴리브롬화디페닐에테르의 소나무 잎, 소나무 껍질 및 토양으로의 침착 특성)

  • Chun, Man Young
    • Journal of Environmental Health Sciences
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    • v.40 no.3
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    • pp.215-224
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    • 2014
  • Objective: This study was carried out in order to determine the depositional characteristics of pine needles, pine bark, and soil used as a passive air sampler (PAS) for atmospheric polybrominated diphenyl ethers (PBDEs). Methods: All three media were sampled from the same site. The PBDE concentrations were analyzed by HRGC/HRMS, and the lipid contents were measured using the gravimetric method by n-hexane extraction. Results: The total PBDE concentration was the highest in soil (22,274.57 pg/g dry), followed by pine bark (20,266.39 pg/g dry), and then pine needles (7,380.22 pg/g dry). Pine needles contained the highest lipid contents (21.31 mg/g dry), whereas soil (10.01 mg/g dry), and pine bark (4.85 mg/g dry) contained less. There were poor correlations between lipid content and total PBDE concentrations in the media ($R^2$=0.8216, p=0.2814). Congeners BDE 47, 99, 183, 196, 197, 206, 207 and 209 showed peak concentrations. Among these, BDE 206, 207, and 209 are highly brominated PBDEs that exist as particulates in ambient air. They accounted for 81.2% [69.2 (pine needles) - 89.0% (tree bark)] of the concentration and therefore are noted as the main congener of the total PBDEs. Conclusions: It can therefore be concluded that for reducing error by improper sampling, the same species of media should be recommended for use as a PAS for atmospheric PBDEs due to the differences in depositional characteristics.

Packaging and Storage of kimchi with Polyethylene Film Contained Raw Ore (생광석 함유 폴리에틸렌 필름을 사용한 김치의 포장저장)

  • 김순동;김미향;김미경
    • Food Science and Preservation
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    • v.5 no.4
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    • pp.355-362
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    • 1998
  • Polyethylene films contained 0, 10, 20, 30 and 40% of raw-ore powder(PERO) were prepared. The characteristics feature of the film and the powder were investigated in order to use packaging material for kimchi quality. Kimchi was packaged in the PERO bass md stored at 10$^{\circ}C$. The kimchi was examined for a pH, acidity, number of total microbe and lactic acid bacteria, E. coli, color values and sensory evaluation. The ore powder at 20$^{\circ}C$ produced infrared rays at 800-1100nm. The growth of E. coli md Staphylococcus aureus was extremely inhibited in the EMB and nutrient broth containing 10% of raw-ore powder but, that of lactobacillus plantarum and Leuconostoc mesenteriodes was slightly promoted in MRS broth containing 1%. The ripening by pH and acidity was slightly accelerated in kimchi in PERO bag(PERO-kimchi) compared to control kimchi but the maintenance of ripened-kimchi taste was prolonged in PERO-kimchi. The number of lactic acid bacteria of PERO-kimchi was more numerous than that of contol sample but that of E. coli wag exremely legs. The color L* values of PERO-kimchi was lower than control but a* and b* values were higher. Sensory evaluation of PERO-kimchi was higher score than control sample in crispness and overall taste about 10 to 20% of raw-ore contents for kimchi-packaging material was desirable.

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The Analysis of Contract-Foodservice Operational Efficiency using Data Envelopment Analysis and Efficiency-Profit Matrix (다점포 운영 푸드서비스 기업의 효율성 측정에 관한 연구 - DEA 및 효율, 수익 매트릭스 분석을 중심으로 -)

  • Kim, Tae-Hee;Park, Ju-Yeon
    • Journal of the East Asian Society of Dietary Life
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    • v.20 no.5
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    • pp.823-835
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    • 2010
  • The research aimed to measure the efficiency of using multi stores in a foodservice company using by DEA (data envelopment analysis) which is a new management science technique. The study also attempted to identify relevant variables affecting DEA efficiency in order to suggest methods for improving efficiency. The data were collected from 148 contract foodservice operations, which were operated in similar fashion in October 2009. The DEA efficiency was calculated as an output-oriented BCC Model. Sales, and CSI (customer satisfaction index) were used as output variables whereas food cost, labor cost, and management expense were used as input variables to calculate the DEA efficiency. Operation process variables of the unit consisted of the were consist of ratio of regular employee, ratio of housekeeper, meal counts, meal price, food cost per meal, contract period, number of menu items, forecasting accuracy, order accuracy, inventory turnover, use of processed food, deviation of food cost, number of new menus, and number of events. According to the BCC score and profitability, units were classified into four groups: High efficiency-high profitability (HEHP), High efficiency-low profitability (HELP), Low efficiency-high profitability (LEHP), and Low efficiency-low profitability (LELP). The HEHP group contained 54 units, which mostly contracted management fee type and had a high meal price. The units were also very large and, served three meals. Twenty of the units were operated with high labor cost: most of these were factories and hospitals. The LEHP group contained 20 units, that were mainly office stores of large scale and medium price. Fifty-four LELP group had a low meal price. A high performance group must have high efficiency, profitability, and satisfaction. The BCC score was over 0.969, the meal price was over 4,116 won, the food cost was over 2,077 won, and meal counts per month were over 10,212 meals.

Assessment of anatomical characteristics of the medicinal plant African cherry (Prunus africana) for its accurate taxonomic identification

  • Komakech, Richard;Yang, Sungyu;Song, Jun Ho;Choi, Goya;Kim, Yong-Goo;Okello, Denis;Omujal, Francis;Kyeyune, Grace Nambatya;Matsabisa, Motlalepula Gilbert;Kang, Youngmin
    • Journal of Plant Biotechnology
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    • v.49 no.2
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    • pp.139-144
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    • 2022
  • The genus Prunus (family: Rosaceae) consists of over 400 plant species and exhibits vast biodiversity worldwide. Given the wide distribution of this genus, its taxonomic classification is important. Anatomical characteristics are conserved and stable and can therefore be used as an important tool for the taxonomic characterization of plants. Therefore, this study aimed to assess and document the anatomical characteristics of the leaf, stem, and seed of P. africana using micrographs and photographs for possible use in the identification, quality control, and phylogenetic analysis of the species. The anatomical sections of a young stem revealed a cortex consisting of isodiametric parenchyma cells, druse crystals, primary vascular bundles, and pith. The mature stem bark majorly consisted of the rhytidome, with the periderm densely arranged in multiple layers; a cluster of stone cells; and sclerenchyma. The leaf sections were hypostomatic, with stomata sizes ranging from 18.90-(22.34)-26.90 × 15.41-(18.40)-21.22 ㎛. The leaf sections showed the presence of characteristic druse crystals, vascular bundles, and mesophyll layers. The pericarp contained the epicarp, mesocarp, and endocarp, with their thickness being approximately 350-400, 300-350, and 30-50 ㎛, respectively. In addition, it contained a seed testa with a thickness of approximately 50-60 ㎛. The morphological and anatomical characteristics observed in P. africana leaves, stems, and seeds in this study could serve as useful data for the taxonomic identification of this species.

Nutritional Components and Physiological Activity of 4 Wild Vegetables (Salvia plebeia R. Br, Angelica acutiloba, Gynura procumbens and Saururus chinensis Baill) Cultivated in Chungbuk Province (충북산 산채 4종(배암차즈기, 일당귀, 명월초 및 삼백초)의 영양성분 및 생리활성)

  • Eom, Hyun-Ju;Jeong, Yu Yeong;Kwon, Nu Ri;Kim, Ki Hyun;Yeon, Eunsol;Yoon, Hyang-Sik;Ryu, Yong-jae;Kim, In Jae
    • The Korean Journal of Food And Nutrition
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    • v.34 no.4
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    • pp.398-406
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    • 2021
  • This study investigated on the nutritional components and physiological activity of four wild vegetables namely Salvia plebeia R. Br., Angelica acutiloba, Gynura procumbens and Saururus chinensis Baill for the development of representative wild vegetables in Chungbuk. Salvia plebeia possessed the highest radical scavenging activity and beta-carotene, but exhibited the lowest α-glucosidase inhibitory activity compared to the other three vegetables. Angelica acutiloba showed high crude protein content and α-glucosidase inhibitory activity, but contained low total polyphenol content, radical scavenging ability and beta-carotene compared to the other three vegetables. Gynura procumbens showed high mineral content, beta-carotene, vitamin K1 content and α-glucosidase inhibitory activity, but showed the lowest total polyphenol content and radical scavenging ability compared to the other three vegetables. Saururus chinensis showed high crude fiber content and total polyphenol content, but contained the lowest mineral and vitamin K1 content. To conclude, it is suggested to use Salvia plebeia or Saururus chinensis as antioxidant food materials and Angelica acutiloba and Gynura procumbens as food materials and sources of α-glucosidase inhibitors. In particular, it is believed that Saururus chinensis, which possessed high content of crude fiber, is suitable for low-calorie food materials such as diet products.

Accuracy of Thoracolumbar Spine K-Wire Placement in Toy, Small and Medium Breed Dogs: Novice Surgeons with 3D Printed Patient-Specific Guide versus an Experienced Surgeon with Freehand Techniques

  • Hwa-Joeng Shin;Hae-Beom Lee;Yoon-Ho Roh
    • Journal of Veterinary Clinics
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    • v.39 no.6
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    • pp.294-301
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    • 2022
  • Three-dimensional (3D) printing technique has been widely used for accurate screw and pin placement in orthopedic surgery and neurosurgery. However, there are few reports comparing the accuracy between the patient-specific guides and freehand Kirschner wire (K-wire) placement in toy, small and medium breed dogs. This study aimed to assess the accuracy of 3D printed patient-specific guides (PSGs) in pin insertion in the thoracolumbar vertebrae of toy breed dogs and compare the outcomes between novice and experienced surgeons. The experiment was conducted on the thoracolumbar vertebrae of 21 euthanized toy breed dogs (median weight, 5.95 kg). The optimal insertion angle placement was determined and patient-specific guides for K-wire insertion were designed and 3D printed using computed tomography (CT) and a 3D computer-aided design program of three vertebrae (Thoracic 12-Lumbar 1). K-wire tracts were made by experienced and novice surgeons and compared to assess the accuracy based on postoperative CT. Based on postoperative CT, in the experienced group, 61 out of 63 pins (96.8%) were fully contained inside the vertebral body and lamina, whereas two pins (3.2%) had perforated the vertebral canal (grade 3, 2-4 mm breach). However, all the pins in the novice group were fully contained. The use of 3D printed PSGs for pin insertion in the thoracolumbar region is an accurate and safe alternative to freehand screw placement by novice surgeons in toy, small and medium breed dogs. Operations with 3D printed PSGs allow novice surgeons to achieve better or similar outcomes in accurate placement of pin/screws in vertebrae.

The Effect of Annealing on sSEBS/Polyrotaxanes Electrolyte Membranes for Direct Methanol Fuel Cells

  • Won, Jong-Ok;Cho, Hyun-Dong;Kang, Yong-Soo
    • Macromolecular Research
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    • v.17 no.10
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    • pp.729-733
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    • 2009
  • Solution casting films of sulfonated poly[styrene-b-(ethylene-r-butylene)-b-styrene] copolymer (sSEBS)-based composite membranes that contained different amounts of organic, nanorod-shaped polyrotaxane were annealed at various temperatures for 1 h. The films' properties were characterized with respect to their use as polymer electrolyte membranes in direct methanol fuel cells (DMFCs). Different aspect ratios of polyrotaxane were prepared using the inclusion-complex reaction between $\alpha$-cyclodextrin and poly(ethylene glycol). The presence of the organic polyrotaxane inside the membrane changed the morphology during the membrane preparation and reduced the transport of methanol. The conductivity and methanol permeability of the composite membranes decreased with increasing polyrotaxane content, while the annealing temperature increased. All of the sSEBS-based, polyrotaxane composite membranes annealed at $140^{\circ}C$ showed a higher selectivity parameter, suggesting their potential usage for DMFCs.