• Journal of Animal Science and Technology
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• 제64권3호
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• pp.588-598
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• 2022

Despite vaccination, equine influenza virus (EIV) and equine herpesvirus (EHV) infections still cause highly contagious respiratory diseases in horses. Recently, concurrent vaccination with EIV and EHV was suggested as a new approach; however, there have been no reports of concurrent vaccination with recombinant canarypox EIV and inactivated EHV vaccines. In this study, we aimed to compare the EIV-specific immune responses induced by concurrent administrations of a recombinant canarypox EIV vaccine and an inactivated bivalent EHV vaccine with those induced by a single recombinant canarypox EIV vaccine in experimental horse and mouse models. Serum and peripheral blood mononuclear cells (PBMCs) were collected from immunized animals after vaccination. EIV-specific serum antibody levels, serum hemagglutinin inhibition (HI) titers, and interferon-gamma (IFN-γ) levels were measured by enzyme-linked immunosorbent assay, HI assay, and quantitative polymerase chain reaction, respectively. Concurrent EIV and EHV vaccine administration significantly increased IFN-γ production, without compromising humoral responses. Our data demonstrate that concurrent vaccination with EIV and EHV vaccines can enhance EIV-specific cellular responses in horses.

• 한국미생물·생명공학회지
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• 제50권3호
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• pp.430-435
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• 2022

The infectious bursal disease (IBD) is a highly contagious and acute poultry disease caused by Birnavirus. However, the vaccination is the only disease prevention, but several factors impeded vaccine development. Thus, a need for time to develop a novel technique for managing and treating respiratory diseases in poultry birds. Passive immunization is a hope and a possible alternative used in birds to meet this need. The current research attempted to produce egg yolk-based polyclonal antibodies against the IBD virus. The benefits of IgY include ease of extraction, lack of reaction with mammalian Fc receptors, and low production cost. Commercial layers were immunized with inactivated IBD virus subcutaneously according to the treatment regimen. The eggs were gathered daily, and yolk antibodies were extracted with the ammonium sulfate precipitation technique. The use of an indirect hemagglutination test demonstrated that IgY was IBD-specific. Until the end of the experiment, the specific IgY immunoglobulins did not lose activity when stored at 4℃. The specific immunoglobulin (IgY) treated challenged birds were demonstrated 92% recovery in comparison to the control group. The study implies that the IBDV specific IgY is an easily prepared and rich source of antibodies and offers an alternative therapeutic agent to cure IBD-infected birds.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송∙미디어공학회 2020년도 하계학술대회
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• pp.630-633
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• 2020

무의식적인 손-얼굴의 접촉으로 인한 감염의 문제점을 해결하기 위해, 얼굴 만지기 행동을 인식할 필요가 있다. 본 연구는 최근 각광을 받는 딥러닝 기술을 이용하여 비디오 영상에서 얼굴 만지기 행동 인식에 대한 연구이다. 우선, 비디오 영상에서 얼굴 만지기와 관련된 11 가지 행동에 대한 시, 공간적 특징을 컨볼루션 신경망을 통해 추출한다. 추출된 정보는 각 행동 레이블로 인코딩되어 비디오 영상에서 얼굴 만지기 행동을 분류한다. 또한, 3D, 2D 컨볼루션 신경망의 대표 네트워크인 I3D, MobileNet v3에 대해 비교 실험을 진행한다. 제안하는 시스템을 적용하여 인간의 행동을 분류하는 실험을 진행했을 때, 얼굴을 만지는 행동을 99%의 확률로 구분했다. 이 시스템을 이용하여 일반인이 무의식적인 얼굴 만지기 행동에 대해서 정량적으로 또는 적시적으로 인식을 하여, 안전한 위생 습관을 확립하여 감염의 확산방지에 도움을 줄수 있기를 바란다.

• Clinical and Experimental Vaccine Research
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• 제12권4호
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• pp.291-297
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• 2023

Enterovirus infections induce infectious diseases in young children, such as hand, foot, and mouth disease which is characterized by highly contagious rashes or blisters around the hands, feet, buttocks, and mouth. This predominantly arises from enterovirus A71 or coxsackievirus A16 infections and in severe cases, they can lead to encephalitis, paralysis, pulmonary edema, or even fatality, representing a global health threat. Due to the absence of effective therapeutic strategies for these infections, various experimental animal models are being investigated for the development of vaccines. During the early stages of research on enterovirus infections, non-human primate infections exhibited symptoms like those in humans, leading to their utilization as model animals. However, due to economic and ethical considerations, their current usage is limited. While enterovirus infections do not readily occur in mice, an infection model with mouse-adapted strain in neonatal mice has been employed. Cellular receptors have been identified in human cells, and genetically modified mice expressing these receptors have been used. Most recently, the utilization of Mongolian gerbil model is actively being considered and should be pursued for further animal model development. So, herein, we provide a summarized overview of the current portfolio of available enterovirus infection models, emphasizing their respective advantages and limitations.

• IMMUNE NETWORK
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• 제21권1호
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• pp.3.1-3.16
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• 2021

Coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been spreading worldwide since its outbreak in December 2019, and World Health Organization declared it as a pandemic on March 11, 2020. SARS-CoV-2 is highly contagious and is transmitted through airway epithelial cells as the first gateway. SARS-CoV-2 is detected by nasopharyngeal or oropharyngeal swab samples, and the viral load is significantly high in the upper respiratory tract. The host cellular receptors in airway epithelial cells, including angiotensin-converting enzyme 2 and transmembrane serine protease 2, have been identified by single-cell RNA sequencing or immunostaining. The expression levels of these molecules vary by type, function, and location of airway epithelial cells, such as ciliated cells, secretory cells, olfactory epithelial cells, and alveolar epithelial cells, as well as differ from host to host depending on age, sex, or comorbid diseases. Infected airway epithelial cells by SARS-CoV-2 in ex vivo experiments produce chemokines and cytokines to recruit inflammatory cells to target organs. Same as other viral infections, IFN signaling is a critical pathway for host defense. Various studies are underway to confirm the pathophysiological mechanisms of SARS-CoV-2 infection. Herein, we review cellular entry, host-viral interactions, immune responses to SARS-CoV-2 in airway epithelial cells. We also discuss therapeutic options related to epithelial immune reactions to SARS-CoV-2.

• 대한예방한의학회지
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• 제10권1호
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• pp.33-51
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• 2006

With the reduction of contagious diseases throughout the world and prolonged life expectancy has lead into increase of habitual related diseases. Industrial development and better economic situation made people more concerned about their health. As primary illnesses are subdued in the past years, health care system and the public value prevention and well-being more than the treatment itself. Based on this trend, this study focused on the view of life from the perspective of Oriental medicine as it's peculiarities, regimen methods, and the definition of healthy life are evaluated. Following results were obtained: - View of life in Oriental medicine focused on two basic principles of interrelationship between the organs internally, inter-dependency with the natural environment and social surrounding externally as recognition and respect between the medium were valued. - Sustaining and maintaining good health in Oriental medicine are closely related to prenatally healthy pregnancy and fetation, as well as prenatal training. Postnatal maintenance includes accommodation to seasonal changes, adequate food intake, mind control, various regimen methods and avoidance of wrong doings. - Defining health includes body's internal condition and external influences, principles of essence, qi, spirit, and yin and yang, harmony and balance, and individuality. - To conclude, good health in Oriental medicine is defined as 'external adaptation to society and natural environment, and internal balancing of individual difference with accumulation of essence, nurturing and circulating qi, and every material around the body in harmony and balance."

• 한국동물위생학회지
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• 제32권2호
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• pp.111-117
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• 2009

Farmed deer could be susceptible carrier to bovine viral infectious disease. But unfortunately, there has not been an overall study over this subject in Korea so far. Therefore, a study was conducted to see serum antibodies to bovine leukosis, food and mouth disease, bovine viral diarrhea and infectious bovine rhinotracheitis in deer using the sera of farmed deer. As a result, two deer in a farms showed positive in bovine leukosis antibodies, using ELISA. For wild water deer, no antibodies were found for those diseases. As a result, it can be assumed that deer were relatively low rate of exposure to highly contagious disease such as viral bovine infectious disease in Korea. As this study was conducted over limited in number of subject and regions, continued study should be carried out in order to prevent and control the interspecies transmission in the future.

• Journal of Korean Medical Science
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• 제33권50호
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• pp.321.1-321.7
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• 2018

Background: Pertussis is highly contagious respiratory disease. Healthcare workers (HCWs) can be an important mediator of the disease. A seroprevalence of pertussis was investigated in HCWs to determine the immune status against pertussis and to detect the unidentified pertussis. Methods: This study was conducted for HCWs at a hospital located in Korea in 2011. After obtaining written informed consent for HCWs voluntarily participating in the study, 10 mL of blood was collected from each subject. Demographic and medical data were collected using questionnaire. Data on the underlying disease and vaccination history were reviewed again through medical records. The presence of anti-pertussis toxin (anti-PT) immunoglobulin G (IgG), and immunoglobulin A (IgA) was detected by quantitative analysis using a commercially available ELISA kit (EUROIMMUN, $L{\ddot{u}}beck$, Germany). Results: A total of 412 HCW participated in the study. Among them, 14 were excluded due to the inadequate sample amount or medical history not secured. Of the 398 HCWs analyzed, 16.6% (66/398) were men and the mean age was $33.82{\pm}9.10years$ (range, 21-67). The mean anti-PT IgG titer was $8.32{\pm}20.40IU/mL$ (range, 0.4-287.5 IU/mL). The overall seroprevalence (rate of anti-PT IgG antibody [Ab] titer > 5 IU/mL) was 33.7%. Three (0.8%) HCWs had the Ab level > 100 IU/mL indicated acute infection. There was no statistically significant difference in the seroprevalence and mean titer of anti-PT IgG Ab according to age group, type of occupation, patient-facing position, or working in the pediatric department. Conclusion: The seroprevalence of pertussis of the HCWs of a university hospital in Korea was low, and there were some unrecognized acute infections. Therefore, booster immunization of pertussis to HCWs should be actively considered.

• 산업과 과학
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• 제1권2호
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• pp.30-36
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• 2022

의학이 고도로 발달한 2022년에도 범유행 감염병은 지속해서 발생하고 있다. 2019년 발생한 코로나-19는 전염성이 강하여 많은 감염자를 발생시키고 있다. 국내에서는 코로나-19의 확산 방지를 위해 선별 진료소 및 호흡기 클리닉 등의 임시 선별 진료소를 증설했지만, 검사를 받기 위한 인구가 몰려 병목현상이 생기고 이에 따라 검사를 받지 못하는 등의 문제점이 있다. 본 연구에서는 문제점을 해결하기 위해 선별 진료소로 코로나-19 검사를 받으려는 사람들을 실시간으로 파악하고자 한다. 실시간 데이터베이스를 활용하여 선별 진료소와 연동하고 사용자에게 선별 진료소별 실시간 현황을 제공한다. 앱을 이용하면 사용자는 실시간으로 선별 진료소 상황을 파악할 수 있고 원활한 진료소를 선택할 수 있다.

• Journal of Veterinary Science
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• 제24권5호
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• pp.55.1-55.12
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• 2023

Background: Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells. Objectives: PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response. Methods: Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4⁺ and CD8⁺ T cells after PPRV infection. Results: PPRV stimulated the differentiation of CD4⁺ and CD8⁺ T cells. In addition, PPRV induced apoptosis in goat PBMCs. Furthermore, apoptosis and the inflammatory response induced by PPRV could be suppressed by Z-VAD-FMK and Z-YVAD-FMK, respectively. Moreover, the virus titer of PPRV was attenuated by inhibiting caspase-1-dependent apoptosis and inflammation. Conclusions: This study showed that apoptosis and the inflammatory response play an essential role in PPR viral replication in vitro, providing a new mechanism related to the cell host response.