Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Inhibition of caspase-1-dependent apoptosis suppresses peste des petits ruminants virus replication

  • Lingxia Li (College of Agriculture and Animal Husbandry, Qinghai University) ;
  • Shengqing Li (College of Animal Science and Veterinary Science, Qinghai University; Qinghai Key Laboratory of Pathogen Diagnosis and Green Prevention and Control Technology for Animal Diseases) ;
  • Shengyi Han (College of Animal Science and Veterinary Science, Qinghai University; Qinghai Key Laboratory of Pathogen Diagnosis and Green Prevention and Control Technology for Animal Diseases) ;
  • Pengfei Li (State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Guoyu Du (State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Jinyan Wu (State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Xiaoan Cao (State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences) ;
  • Youjun Shang (State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
  • Received : 2022.11.14
  • Accepted : 2023.05.26
  • Published : 2023.09.30
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Abstract

Background: Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells. Objectives: PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response. Methods: Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4+ and CD8+ T cells after PPRV infection. Results: PPRV stimulated the differentiation of CD4+ and CD8+ T cells. In addition, PPRV induced apoptosis in goat PBMCs. Furthermore, apoptosis and the inflammatory response induced by PPRV could be suppressed by Z-VAD-FMK and Z-YVAD-FMK, respectively. Moreover, the virus titer of PPRV was attenuated by inhibiting caspase-1-dependent apoptosis and inflammation. Conclusions: This study showed that apoptosis and the inflammatory response play an essential role in PPR viral replication in vitro, providing a new mechanism related to the cell host response.

Keywords

Acknowledgement

This research was supported by the National Key R & D plan (2022YFD1602203), the Gansu Provincial Science and Technology Special Mission (22CX8NA012), the Special Project on Transformation of Scientific and Technological Achievements (2022-NK-118), and the Qinghai Provincial Science and Technology Plan (2021-ZJ-923).

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