• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.45-45
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• 2014

Individualities of precious health mushroom called Zhenghonggu@ from respective protections scattered among all main mountains of Fujian China were collected and recognized locally, then compared with Russula griseocarnosa. Their internal transcribed spacer (ITS) region (ITS1, ITS2 and 5.8S rDNA) of the nuclear rDNA were amplified, AMOVA analyzed, nested clade analyzed and then compared with the ITS sequences of relative Russula species from other regions of China to confirm the taxonomic status of Zhenghonggu$^@$ and its population structure. Total 23 haplotypes from different protections of Fujian can be clustered into three clades similar to the three lineages of Dahongjun$^@$ from southeastern China reported by Li et al. The geographic distribution characteristic of these three phylogeny clades may be closely coupled with the vegetation regionalization and/or the differences of coenosium construction of Fagaceae that is the host of Russula griseocarnosa. The correlation of taxonomy, phylogeny and geographical distribution of Russula are discussed.

• Parasites, Hosts and Diseases
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• v.44 no.1 s.137
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• pp.63-66
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• 2006

Huge numbers of non-biting midges emerged from brackish water which were made at the harbor construction field in Jinhae City, Kyongsangnam-do, Korea in late summer in 2005, and caused a serious nuisance to villagers. The midges were collected and identified as Chironomus salinarius (Kieffer, 1921). Although this species was recorded in Korea for the first time in 1998, the morphological descriptions were so brief and simple. A full redescription is made with detailed illustrations for ecological and control workers of this nuisance midge.

• 한국IT서비스학회:학술대회논문집
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• 2009.05a
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• pp.110-114
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• 2009

최근 정보기술과 유비쿼터스기술을 바탕으로 도시를 미래화하는 유비쿼터스도시(U-City)구축이 범 국가적으로 추진되고 있다. 이에, 정부에서는 2008년 9월 25일 유비쿼터스도시의 건설 등에 관한 법률 시행령을 발표하였으며, 유비쿼터스도시서비스(U-City Service)를 크게 행정, 교통, 보건 의료 복지, 환경, 방범 방재, 시설물 관리, 교육, 문화 관광 스포츠, 물류, 근로 고용 등의 분야로 구분하였다. 이러한 유비쿼터스도시서비스를 제공하고 운영 및 관리하는 시설로 유비쿼터스도시 통합운영센터(U-City Management Center)는 다양한 유비쿼터스도시서비스를 효과적으로 연계하여 서비스에서 발생되는 다양한 도시정보를 통합, 분석하고 제공하는 유비쿼터스도시 핵심기능을 담당한다. 하지만 이러한 유비쿼터스도시 통합운영센터의 구축은 운영조직구성, 운영비용확보, 통합서비스 제공방안, 법 제도 정비 등의 해결과제를 가지고 있어 구현방안에 대한 구체적인 연구가 요구된다. 본 연구에서는 선행연구를 바탕으로 유비쿼터스도시 통합운영센터에서 기본적으로 제공되어야 할 유비쿼터스도시서비스 유형을 제시하고 정의한다.

• Korean Journal of Plant Taxonomy
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• v.51 no.4
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• pp.386-390
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• 2021

Petrorhagia nanteuilii (Burnat) P. W. Ball & Heywood (Caryophyllaceae), native to western Europe and western North Africa and an introduced or alien plant in South America, Australia, and Japan, was newly found in Gyeongsangnam-do, Korea. Petrorhagia is clearly distinguished from other genera by a bract at the base of calyx, which differs from Gypsophila L., and commissures at the sepals, which is different from Dianthus L. Petrorhagia nanteuilii grows on slopes and roadsides, suggesting that it is likely to have been introduced through the installation of green sites and road construction. A precise description, illustration, photographs, and a key to related genera and species is provided.

• Journal of The Korean Association For Science Education
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• v.39 no.2
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• pp.295-305
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• 2019

In previous study, visual representation competence taxonomy (VRC-T), which is composed of two dimensions, was developed for the purpose of promoting effective visual representation use and research in science education. In this study, elementary school students' visual representation competence for shadow phenomenon was investigated using VRC-T. In terms of visual representation competence, 'interpretation' was the highest score, followed by 'construction' and 'integration'. It also showed that students' visual representation competence was not high even after learning shadow-related units in the regular curriculum. On the other hand, text-based scientific knowledge was not correlated with all categories of visual representation competence. This indicates that there is a need to emphasize visual representation more in science class. Finally, hierarchical relationship among cognitive processes of VRC-T was explored according to ordering theory. If the tolerance level is somewhat loosened, a linear hierarchical relationship was found between the six cognitive processes. This suggests that VRC-T is an analytical framework that can be useful when designing assessment tools, tasks, and science class activities to enhance visual representation competence.

• Korean Journal of Plant Taxonomy
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• v.41 no.3
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• pp.182-193
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• 2011

As a part of the on-going effort to conserve endangered Scrophularia takesimensis Nakai in Korea, its genetic structure and diversity from 3 population, consisted of 14 subpopulations in Ulleung Island were analyzed using RAPD band patterns. Out of 60 primers tested, 33 generated amplified bands with its genome, including 149 polymorphic and 67 monomorphic bands. The highest number (146) was found in northern population, especially, 64 in HY subpopulation; the smallest (40) in eastern population. An examination of its genetic structure with AMOVA revealed that about 60% of all variations could be assigned to among subpopulations within populations. Population differentiation among populations and subpopulations is seriously going now because of habitat fragmentation due to human activities, such as road and small port construction. Although the habitats of S. takesimensis in Ulleung Island, Korea are disappeared at an alarming rate, significant levels of genetic variation still exist at species level, and population level, especially northern population. Therefore, three conservation strategies should be needed urgently; 1) preservation of populations as it stands, 2) establishment of recovery plan to connect population and subpopulations genetically, and 3) long-term monitoring.

• International Journal of Control, Automation, and Systems
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• v.3 no.2
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• pp.183-194
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• 2005

In this study, we introduce an advanced architecture of genetically optimized Hybrid Fuzzy Neural Networks (gHFNN) and develop a comprehensive design methodology supporting their construction. A series of numeric experiments is included to illustrate the performance of the networks. The construction of gHFNN exploits fundamental technologies of Computational Intelligence (CI), namely fuzzy sets, neural networks, and genetic algorithms (GAs). The architecture of the gHFNNs results from a synergistic usage of the genetic optimization-driven hybrid system generated by combining Fuzzy Neural Networks (FNN) with Polynomial Neural Networks (PNN). In this tandem, a FNN supports the formation of the premise part of the rule-based structure of the gHFNN. The consequence part of the gHFNN is designed using PNNs. We distinguish between two types of the linear fuzzy inference rule-based FNN structures showing how this taxonomy depends upon the type of a fuzzy partition of input variables. As to the consequence part of the gHFNN, the development of the PNN dwells on two general optimization mechanisms: the structural optimization is realized via GAs whereas in case of the parametric optimization we proceed with a standard least square method-based learning. To evaluate the performance of the gHFNN, the models are experimented with a representative numerical example. A comparative analysis demonstrates that the proposed gHFNN come with higher accuracy as well as superb predictive capabilities when comparing with other neurofuzzy models.

• The Korean Journal of Malacology
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• v.31 no.3
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• pp.243-247
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• 2015

A stand-alone BLAST server is available that provides a convenient and amenable platform for the analysis of molluscan sequence information especially the EST sequences generated by traditional sequencing methods. However, it is found that the server has limitations in the annotation of molluscan sequences generated using next-generation sequencing (NGS) platforms due to inconsistencies in molluscan sequence available at NCBI. We constructed a web-based interface for a new stand-alone BLAST, called PANM-DB (Protostome DB) for the analysis of molluscan NGS data. The PANM-DB includes the amino acid sequences from the protostome groups-Arthropoda, Nematoda, and Mollusca downloaded from GenBank with the NCBI taxonomy Browser. The sequences were translated into multi-FASTA format and stored in the database by using the formatdb program at NCBI. PANM-DB contains 6% of NCBInr database sequences (as of 24-06-2015), and for an input of 10,000 RNA-seq sequences the processing speed was 15 times faster by using PANM-DB when compared with NCBInr DB. It was also noted that PANM-DB show two times more significant hits with diverse annotation profiles as compared with Mollusks DB. Hence, the construction of PANM-DB is a significant step in the annotation of molluscan sequence information obtained from NGS platforms. The PANM-DB is freely downloadable from the web-based interface (Malacological Society of Korea, http://malacol.or/kr/blast) as compressed file system and can run on any compatible operating system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.7
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• pp.312-320
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• 2020

The objective of the study was to undertake a phylogenetic diversity census of ruminal archaea based on a meta-analysis of 16S rRNA gene sequences that were publicly available in the Ribosomal Database Project. A total of 8,416 sequences were retrieved from the Ribosomal Database Project (release 11, update 5) and included in the construction of a taxonomy tree. Species-level operational taxonomic units (OTUs) were analyzed at a 97% sequence similarity by using the QIIME program. Of the 8,416 sequences, 8,412 were classified into one of three phyla; however, the remaining four sequences could not be classified into a known phylum. The Euryarchaeota phylum was predominant and accounted for 99.8% of the archaeal sequences examined. Among the Euryarchaeota, 65.4% were assigned to Methanobrevibacter, followed by Methanosphaera (10.4%), Methanomassillicoccus (10.4%), Methanomicrobium (7.9%), Methanobacterium (1.9%), Methanimicrococcus (0.5%), Methanosarcina (0.1%), and Methanoculleus (0.1%). The 7,544 sequences that had been trimmed to the V2 and V3 regions clustered into 493 OTUs. Only 17 of those 493 OTUs were dominant groups and accounted for more than 1% of the 7,544 sequences. These results can help guide future research into the dominant ruminal methanogens that significantly contribute to methane emissions from ruminants, research that may lead to the development of anti-methanogenic compounds that inhibit these methanogens regardless of diet or animal species.

• Journal of Marine Bioscience and Biotechnology
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• v.11 no.2
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• pp.52-61
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• 2019

Over the past few decades, microalgae-based biotechnology conjugated with innovative CRISPR/Cas9-mediated genetic engineering has been attracted much attention for the cost-effective and eco-friendly value-added compounds production. However, the discharge of reproducible living modified organism (LMO) into environmental condition potentially causes serious problem in aquatic environment, and thus it is essential to assess potential environmental risk for human health. Accordingly, in this study, we monitored discharged genetically modified microalgae (GMM) near the research complex which is located in Daejeon, South Korea. After testing samples obtained from 6 points of near streams, several green-colored microalgal colonies were detected under hygromicin-containing agar plate. By identification of selection marker genes, the GMM was not detected from all the samples. For the lab-scale environmental risk assessment of GMM, acute toxicity test using rotifer Brachionus calcyflorus was performed by feeding GMM. After feeding, there was no significant difference in mortality between WT and transformant Chlamydomonas reinhardtii. According to further analysis of horizontal transfer of green fluorescence protein (GFP)-coding gene after 24 h of incubation in synthetic freshwater, we concluded that the GFP-expressed gene not transferred into predator. However, further risk assessments and construction of standard methods including prolonged toxicity test are required for the accurate ecological risk assessment.