• Computers and Concrete
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• 제10권3호
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• pp.307-330
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• 2012

This work intends to contribute for the improvement of the procedure suggested by Brazilian Technical Code that takes into account the cracked concrete stiffness in the estimative of the displacement of reinforced concrete beams submitted to service loads. A damage constitutive model accounting for induced anisotropy, plastic deformations and bimodular elastic response is used in order to simulate the concrete behaviour, while an elastoplastic behaviour is admitted for the reinforcement. The constitutive models were implemented in a program for bars structures analysis with layered finite elements. Initially, the damage model is briefly presented as well as the parametric identification of the materials that have been used in the reinforced concrete beams. After that, beams with different geometries and reinforcement area are analyzed and a statistical method (ANOVA) is employed in order to identify the main variables in the problem. Soon after, the same procedure is used with another resistance of concrete, where the compression strength is changed. The numerical responses are compared with the ones obtained by Brazilian Technical Code and experimental tests in order to validate the use of the damage model. Finally, some remarks are discussed based on responses presented in this work.

• Journal of Life Science
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• 제9권1호
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• pp.40-44
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• 1999

Glutathione S-transferase (GST) is a multifunctional protein that catalyzes the catalyzes the conjugation of glutathione with electrophilic compounds. It exists in a variety of isoenzy-matic froms with a wide range of substrate specificity and plays a pivotal role in detoxification of various drugs. In order to elucidate the GST-${\pi}$'s involvement of multidrug resistance (MDR) in drug-resistant tumor cell lines, we determined GST-${\pi}$ content by "1 step sandwich method". Consequently, adriamycin resistant cells of MCF-7 (MCF-7/ADM) have 7-fold increase of GST-${\pi}$ content than that of MCF-7 cells, while its {TEX}$IC_{50}${/TEX} was 116-fold greater than parent cell line. By northrn blotting, we compared whether MCF-7/ADM cells express GST-${\pi}$ mRNA. The GST-${\pi}$ mRNA expression in these cells was not inducible, but constitutive when treated for 24 h with a concentration of 0, 20, 200, and 2000 nM of adriamycin, respectively. Taken together, these results suggest that GST-${\pi}$ may not be directly associated with multidrug resistance in these human cancer cell lines.ell lines.

• Smart Structures and Systems
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• 제25권3호
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• pp.311-322
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• 2020

This paper studies the influence of parameters of a novel SMA helical spring energy dissipation brace on the seismic resistance of a frame structure. The force-displacement relationship of the SMA springs is established mathematically based on a multilinear constitutive model of the SMA material. Four SMA helical springs are fabricated, and the force-displacement relationship curves of the SMA springs are obtained via tension tests. A numerical dynamic model of a two-floor frame with spring energy dissipation braces is constructed and evaluated via vibration table tests. Then, two spring parameters, namely, the ratio of the helical spring diameter to the wire diameter and the pre-stretch length, are selected to investigate their influences on the seismic responses of the frame structure. The simulation results demonstrate that the optimal ratio of the helical spring diameter to the wire diameter can be found to minimize the absolute acceleration and the relative displacement of the frame structure. Meanwhile, if the pre-stretch length is assigned a suitable value, excellent vibration reduction performance can be realized. Compared with the frame structure without braces, the frames with spring braces exhibit highly satisfactory seismic resistance performance under various earthquake waves. However, it is necessary to select an SMA spring with optimal parameters for realizing optimal vibration reduction performance.

• Computers and Concrete
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• 제21권6호
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• pp.637-647
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• 2018

Numerical approach using finite element method has been used to evaluate the behaviour of reinforced concrete frame structure subjected to fire. The structure is previously designed in accordance with Eurocode standards for the design of structures for earthquake resistance, for the ductility class M. Thermal and structural response are obtained using a commercially available software ANSYS. Temperature-dependent nonlinear thermal and mechanical properties are adopted according to Eurocode standards, with the application of constitutive model for the triaxial behaviour of concrete with a smeared crack approach. Discrete modelling of concrete and reinforcement has enabled monitoring of the behaviour at a global, as well as at a local level, providing information on the level of damage occurring during fire. Critical regions in frame structures are identified and assessed, based on temperatures, displacements, variations of internal forces magnitudes and achieved plastic deformations of main reinforcement bars. Parametric analyses are conducted for different fire scenarios and different types of concrete aggregate to determine their effect on global deformations of frame structures. According to analyses results, the three-dimensional finite element model can be used to evaluate the insulation and mechanical resistance criteria of reinforced concrete frame structures subjected to nominal fire curves.

• 미생물학회지
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• 제24권4호
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• pp.341-351
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• 1986

항생제에 대하여 다중 저항성을 갖는 Shaphylococcus aµreus D-H-1으로부터 chloram phenicol(Cm) 저항성 유전자를 가진것으로 확인된 R-plasmid(pSBK203, 2.5MdaJ.)를 분리하여 Bacillus subtilis BD 170 내에셔 발현시켰으며 이 Cm 저항성 유전자를 cloning하기 위하여 pSBK203상의 제한효소 인식부위를 결정하였다. pSBK203 상의 TaqI 부분 절편 0.3 kb을 pBD9 CZaI 인식부위내에 삽입하여 얻은 재조합 plasmid(pTQ16)와 TaqI부 분절편과 O.lkb 엇갈럼이 있는 RsaI 단일절떤(1. 3 kb} pBR322의 Seal 인식부위에 삽입하여 얻은 재조합 p plasmid(pHW20)에서 Cm 저항성이 획득되었다. pBD9 및 pBR322 상에 삽입된 두 절펀내에 Hinf, Taq I 빛 BglII의 제한효소 인식부위가 존재하였으며 이들 중 행III 인식부위에 의하여 Cm 저항성이 불활성 되었다.

• Computers and Concrete
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• 제29권2호
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• pp.81-92
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• 2022

The influence of normal stress perpendicular to the potential shear plane was always neglected in existing researches, which may lead to a serious deviation of the shear strength of concrete members in practice designs and numerical analyses. In this study, a series of experimental studies are carried out in this paper, which serves to investigate the shear behavior of concrete under compression shear loading. Based on the test results, a three-phase shear failure model for cohesive elements are developed, which is able to take into consideration the influence of normal stress on the shear strength of concrete. To identify the accuracy and applicability of the proposed model, numerical models of a double-noted concrete plate are developed and compared with experimental results. Results show that the proposed constitutive model is able to take into consideration the influence of normal stress on the shear strength of concrete materials, and is effective and accurate for describing the complex fracture of concrete, especially the failure modes under compression shear loadings.

• Journal of Ecology and Environment
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• 제36권1호
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• pp.39-47
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• 2013

The expression of transgenic traits in genetically modified crops is sometimes associated with decreases in crop performance or fitness. These decreases in performance or fitness of transgenic plants in unfavourable conditions may provide valuable information about the ecological consequences of transgene escape. In a glasshouse trial, we tested the cost associated with resistance to herbicides by comparing the growth, yield, and competitive ability of transgenic rice with its parental non-transgenic line. This new line was developed for constitutive overexpression of protoporphyrinogen oxidase (PPO) to increase resistance to herbicides. We evaluated nine agronomic traits of transgenic and non-transgenic rice grown in a replacement series design over four densities. Competitive ability was also assessed between transgenic and non-transgenic plants by analyzing their relative yields based on biomass and seed weight data. Our results indicated that non-transgenic plants showed greater performance than did the transgenic plants when those genotypes were grown in mixtures. The non-transgenic rice plants exhibited superior competitive ability at certain combinations of planting densities and genotype proportions. These results suggest that PPO-herbicide resistance incurs some costs in plant performance and competitive ability.

• 한국지반공학회논문집
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• 제21권2호
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• pp.47-55
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• 2005

본 연구에서는 점토지반에서 수평력을 받는 군말뚝과 단말뚝의 수평저항력을 파악하기 위하여 유한요소 해석프로그램인 ABAQUS를 이용하여 수치해석을 행하였다. 수치해석은 말뚝직경(1.0, 0.5m), 말뚝길이(7, 10m) 그리고 두부조건(두부자유와 말뚝캡을 적용한 두부구속조건)을 변수로 하여 실시하였다. 수평력 작용시 선말뚝(leading pile)의 캡에 의한 영향과,군말뚝내의 각각의 말뚝에 대한수평저항력의 크기와 분포를 평가하기 위하여 1$\times$3 군말뚝을 사용하였다. 점토지반은 Cam-clay 모델을 사용하였고, 말뚝은 원형의 콘크러트로 탄성모델을 사용하여 3차원 해석을 수행하였다. 해석결과 말뚝캡의 크기는 단말뚝의 수평저항력에 영향을 미치는 것으로 나타났으며, 군말뚝내의 선말뚝은 군말뚝의 효과에 의해 수평저항력이 증가하면서 Brown이 제안한p-multiplier 값이 1보다 크게 평가되었다.

• 약학회지
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• 제51권2호
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• pp.108-114
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• 2007

The predominant Macrolides-Lincosamide-Streptogramin B (MLS$_B$) antibiotics resistance genes in staphylococci are erm(A) and erm(C). There is the phenomenon that the ratio of constitutively MLS$_B$ antibiotics resistance (cMLS) in erm(A) is much higher than in erm(C). Thus, we confirmed that the difference of the mutation ratio between erm(A) and erm(C) makes the phenomenon. We examined 8 staphylococci carrying inducibly expressed (iMLS) erm(A) or erm(C) genes. After overnight incubation in the presence of the non-inducer MLS$_B$ antibiotics, spontaneous mutants constitutively expressed MLS$_B$ resistance were selected. Against our expectation, the mutation ratio of erm(A) was lower than erm(C). Therefore, possibilities of other factors determining the ratio of cMLS phenotype might be concerned. All the mutants showed sequence alterations in translational attenuator and all the alterations seemed to give rise to change the second structure of mRNA to express constitutively. For erm(A), 4 different types of sequence deletions ranging from 72 bp to 122 bp and 3 different types of duplications ranging 24 bp to 93 bp were detected. Also, there were 9 different types of duplications ranging 15bp to 154bp in erm(C).

• Steel and Composite Structures
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• 제47권2호
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• pp.269-287
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• 2023

The WGJ420 fire-resistant weathering (FRW) steel is developed and manufactured with standard yield strength of 420 MPa at room temperature, which is expected to significantly enhance the performance of steel structures with excellent fire and corrosion resistances, strong seismic capacity, high strength and ductility, good resilience and robustness. In this paper, the mechanical properties of FRW steel plates and buckling behavior of columns are investigated through tests at elevated temperatures. The stress-strain curves, mechanical properties of FRW steel such as modulus of elasticity, proof strength, tensile strength, as well as corresponding reduction factors are obtained and discussed. The recommended constitutive model based on the Ramberg-Osgood relationship, as well as the relevant formulas for mechanical properties are proposed, which provide fundamental mechanical parameters and references. A total of 12 FRW steel welded I-section columns with different slenderness ratios and buckling load ratios are tested under standard fire to understand the global buckling behavior in-depth. The influences of boundary conditions on the buckling failure modes as well as the critical temperatures are also investigated. In addition, the temperature distributions at different sections/locations of the columns are obtained. It is found that the buckling deformation curve can be divided into four stages: initial expansion stage, stable stage, compression stage and failure stage. The fire test results concluded that the residual buckling capacities of FRW steel columns are substantially higher than the conventional steel columns at elevated temperatures. Furthermore, the numerical results show good agreement with the fire test results in terms of the critical temperature and maximum axial elongation. Finally, the critical temperatures between the numerical results and various code/standard curves (GB 51249, Eurocode 3, AS 4100, BS 5950 and AISC) are compared and verified both in the buckling resistance domain and in the temperature domain. It is demonstrated that the FRW steel columns have sufficient safety redundancy for fire resistance when they are designed according to current codes or standards.