• Journal of Microbiology
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• v.45 no.3
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• pp.227-233
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• 2007

In budding yeast, septin plays as a scaffold to recruits protein components and regulates crucial cellular events including bud site selection, bud morphogenesis, Cdc28 activation pathway, and cytokinesis. Phosphorylation of Bni5 isolated as a suppressor for septin defect is essential to Swe1-dependent regulation of bud morphogenesis and mitotic entry. The mechanism by which Bni5 regulates normal septin function is not completely understood. Here, we provide evidence that Bni5 phosphorylation is important for interaction with septin component Cdc11 and for timely delocalization from septin filament at late mitosis. Phosphorylation-deficient bni5-4A was synthetically lethal with $hof1{\Delta}$. bni5-4A cells had defective structure of septin ring and connected cell morphology, indicative of defects in cytokinesis. Two-hybrid analysis revealed that bni5-4A has a defect in direct interaction with Cdc11 and Cdc12. GFP-tagged bni5-4A was normally localized at mother-bud neck of budded cells before middle of mitosis. In contrast, at large-budded telophase cells, bni5-4A-GFP was defective in localization and disappeared from the neck approximately 2 min earlier than that of wild type, as evidenced by time-lapse analysis. Therefore, earlier delocalization of bni5-4A from septin filament is consistent with phosphorylation-dependent interaction with the septin component. These results suggest that timely de localization of Bni5 by phosphorylation is important for septin function and regulation of cytokinesis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.1
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• pp.31-37
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• 1993

To analyze and classify the varietal variation of alkali digestibility in detail, which is closely connected with the gelatinization temperature and physical characteristics of cooked rice, the patterns of alkali decomposition changed along the alkali concentration were investigated for thirty three Korean leading rice cultivars and new breeding lines(japonica : 25, Tongil-type:8) including five glutinous rice. Principal component analysis was used to condense the information and to classify rice materials according to decomposed reaction pattern at several levels of potassium hydroxide(KOH) concentration. Thirty three rice varieties were classified largely into four groups by the distribution on the plane of upper two principal component scores which contained above 92% of total informations. Group I was consisted of one variety, Dobongbyeo, which owned almost same strong resistance to alkali digestion at the range of 0.8% to 1.6% KOH solutions. Group II included three japonica and Tongil-type glutinous rice varieties, which revealed medium alkali digestion value(ADV) at 1.4% KOH solution and intermediate change in ADV from 0.8% to 1.6% KOH solutions. Most of Tongil-type and early-maturity japonica rice, which exhibited medium-high ADV at 1.4% of KOH concentration and large ADV difference between low and high alkali solutions, were contained in Group III. Group N included most of medium or medium-late-maturity japonica, which showed high ADV at 1.4% KOH and medium or intermediate-high ADV change between low and high alkali solutions. The 1st principal component indicated the average index of ADV through 0.8-1.6% KOH solutions and the 2nd principal component pointed out the factor related with ADV difference between low and high alkali solutions or regression coefficients of ADV change along with the KOH concentrations.

• Transactions of the Korean Society of Automotive Engineers
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• v.22 no.3
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• pp.97-104
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• 2014

Automobile trunk latches enable trunks to be opened and closed by a latch mechanism, which can be selectively positioned between a locked condition and an open condition. To maintain structural and electronic performance of the trunk latch, the latch needs to endure impact load that occurs in its open and close motion, and a dynamic mechanism needs to be electronically controled by a contact switch connected with a small DC motor. A base plate, which is the most important component relating to the structural safety, commonly uses a high stiffness material SAPH440-P with high manufacturing cost. In this paper, through structural analysis and optimization, production cost is significantly reduced by replacing SAPH440-P used in some region of the base plate with engineering plastic PBT GF 20%. The optimized contact switch reduces difference between distributed pressures of its two legs, which leads to improve the electronic performance of the trunk latch.

• Journal of the Korean Society of Industry Convergence
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• v.24 no.5
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• pp.581-587
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• 2021

In recent years, contamination of drinking water sources has emerged as a serious social problem, such as a large number of impurities in tap water or groundwater or the supply of suitable water due to rust of pipes. Although the government and public institutions are implementing various measures to protect water sources, they cannot improve water quality in a short period of time because of the enormous cost involved. Therefore, in recent years, preference has been given to a device that converts tap water, which is hard water, into soft water by installing a separate water softener at the faucet from which tap water is discharged. However, the existing filtration device has a problem that filtration performance is gradually lowered when impurities accumulate in the filter, requiring continuous filter replacement. In this study, the optimal design of the filter housing was performed to develop a water softener that can be washed when impurities accumulate on the filter inside the water softener connected to the faucet. For optimal design of the filter housing, fluid and fluid-structural interaction analysis were performed on the design pressure to determine the shape and thickness of the housing, and design review was performed through prototype.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.20 no.7
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• pp.41-47
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• 2021

In this study, the hinges of heavy weight doors were designed and analyzed in line with the trend that built-in appliances are becoming larger and the weight of doors is also increasing. The main specification of the heavy weight door hinge is to allow the deflection at the end of the door to be less than 2 mm when opening and closing, including the automatic closing, slow closing, and closing force control functions. The structural analysis of the design mechanism, component design, and methods for improving the deflection are as follows: 1) Mechanism of the automatic closing function should sense automatically using the spring compression force at a specific angle by the contact between the cam and the cam module roller. 2) Through structural analysis, the maximum stress of the door was found in the link pin hole connected to the pin at each link. 3) Consequently, the pin holder was designed and applied, with little variance, but up to 93% of the specification limit.

• Progress in Superconductivity and Cryogenics
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• v.25 no.1
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• pp.6-10
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• 2023

2 K superfluid helium cryogenic system is the crucial component of many large accelerators. When the cryogenic system is operating at 2K@3129Pa, many room-temperature parts are connected to superfluid helium via tubes. Air Leakage in these connections may lead to air contamination of the cryogenic system. Air contamination may cause equipment failure in cryogenic systems and, in extreme cases, render the entire accelerator system inoperable. Helium guard is a technique that guards against air contamination of these sub-atmospheric pressure connections in 2 K superfluid helium cryogenic system. This paper introduces a typical 2 K cryogenic distribution design for large accelerators, and make risk analysis of air contamination. Finally, the analysis of specific leakage points and detailed engineering design are presented, which may be used as a reference when designing of a 2 K superfluid helium cryogenic distribution system.

• The Journal of the Korea Contents Association
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• v.13 no.10
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• pp.19-26
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• 2013

In the Malaysian license plates, alphabets and numerals which are made by plastic, are adhered to a frame as embossing style and occasionally characters in horizontal, vertical directions are aligned with narrow space. So the extraction of character stroke information can be hard in the vehicle images of low illumination intensity. In this paper, Malaysian license plate recognition algorithm for low illumination intensity image is proposed. DoG filtering based character stroke generation method is introduced to derive exact connected components of strokes in the vehicle image of low illumination intensity. After localization of plate by connected component analysis, characters are segmented and recognized. Algorithm is experimented for the 6,046 vehicle images captured in Kuala Lumpur by IR camera without using any special light during day and night. The experimental results show that recognition accuracy of plates is 96.1%.

• Horticultural Science & Technology
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• v.32 no.5
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• pp.684-693
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• 2014

Abiotic stress conditions such as cold, drought, and salinity trigger physiological and morphological changes and yield loss in plants. Hence, plants adapt to adverse environments by developing tolerance through complex regulation of genes related to various metabolic processes. This study was conducted to construct a coexpression network for multidirectional analysis of salt-stress response genes in Brassica rapa (Chinese cabbage). To construct the coexpression network, we collected KBGP-24K microarray data from the B. rapa EST and microarray database (BrEMD) and performed time-based expression analyses of B. rapa plants. The constructed coexpression network model showed 1,853 nodes, 5,740 edges, and 142 connected components (correlation coefficient > 0.85). On the basis of the significantly expressed genes in the network, we concluded that the development of salt tolerance is closely related to the activation of $Na^+$ transport by reactive oxygen species signaling and the accumulation of proline in Chinese cabbage.

• Journal of KIISE:Software and Applications
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• v.33 no.11
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• pp.942-952
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• 2006

We propose an automatic segmentation method for identifying pulmonary structures using gray-level information of chest CT images. Our method consists of following five steps. First, to segment pulmonary structures based on the difference of gray-level value, we select the threshold using optimal thresholding. Second, we separate the thorax from the background air and then the lungs and airways from the thorax by applying the inverse operation of 2D region growing in chest CT images. To eliminate non-pulmonary structures which has similar intensities with the lungs, we use 3D connected component labeling. Third, we segment the trachea and left and right mainstem bronchi using 3D branch-based region growing in chest CT images. Fourth, we can obtain accurate lung boundaries by subtracting the result of third step from the result of second step. Finally, we select the threshold in accordance with histogram analysis and then segment radio-dense pulmonary vessels by applying gray-level thresholding to the result of the second step. To evaluate the accuracy of proposed method, we make a visual inspection of segmentation result of lungs, airways and pulmonary vessels. We compare the result of the conventional region growing with the result of proposed 3D branch-based region growing. Experimental results show that our proposed method extracts lung boundaries, airways, and pulmonary vessels automatically and accurately.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.6
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• pp.728-735
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• 2017

With the proliferation of digital devices, the devices have generated considerable additive white Gaussian noise while acquiring digital images. The most well-known denoising methods focused on eliminating the noise, so detailed components that include image information were removed proportionally while eliminating the image noise. The proposed algorithm provides a method that preserves the details and effectively removes the noise. In this proposed method, the goal is to separate meaningful detail information in image noise environment using the edge strength and edge connectivity. Consequently, even as the noise level increases, it shows denoising results better than the other benchmark methods because proposed method extracts the connected detail component information. In addition, the proposed method effectively eliminated the noise for various noise levels; compared to the benchmark algorithms, the proposed algorithm shows a highly structural similarity index(SSIM) value and peak signal-to-noise ratio(PSNR) value, respectively. As shown the result of high SSIMs, it was confirmed that the SSIMs of the denoising results includes a human visual system(HVS).