• 한국미생물·생명공학회지
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• 제19권2호
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• pp.140-146
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• 1991

Mutation experiments were performed to select the mutant of Aspergillus niger 55, which had lost almost all the ability to produce transglucosidases but retained that of high productivity of raw meal saccharifying enzyme, by means of successive induction with N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), ultraviolet(UV) light, and ${\gamma}$-rays. Also, we used the mutant enrichment techniques, such as liquid culture-filtration procedure and differential heat sensitivity of conidia, in order to increase the possibility of obtaining a mutant. The glucoamylase productivity of mutant PFST-38 was 11 times higher than that of the parent strain. The mutant PFST-38 was morphologically identical to the parent strain, except for the size of conidia, the tendency to form conidia and the lenght of conidiophore. Asp. niger mutant PFST-38 apeared to be useful for the submerged production of the raw corn meal saccharifying enzyme.

• 식물병연구
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• 제19권4호
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• pp.308-312
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• 2013

The popcorn disease caused by sclerotia forming fungi reduces the productivity of mulberry fruits in world wide. In Korea, only two species (Ciboria shiraiana and Scleromitrula shiraiana) have been reported as the major causal organisms and their morphological features are also largely unknown. Hereby, we report the first identification of another species (i.e. Ciboria carunculoides) in Korea and detailed features of their anamorphic stage. Fungi dominantly associated with sclerotia were purely isolated from infected mulberry fruits under the microscope. PCR-amplified DNA encoding 5.8S rRNA displayed 100% similarity to Ciboria carunculoides. The anamorphic features exhibited the absence of true mycelia. Instead, very short, aseptated, branched conidiophores were directly emerged from sclerotia. Phialides were usually three in number from each conidiophore, ampuliform to navicular in shape, slightly curved and tapering towards the apex. Conidia were produced from phialides and mostly found as one celled, pear shaped, not hyaline with smooth to uneven surface walled. Diversely modified features in phialides formed pseudo-mycelial structures around the host tissue. Combined all, current study is the first report of C. carunculoides isolated in Korea and the foremost detailed description of its anamorph stage.

• 한국균학회지
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• 제8권3호
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• pp.159-166
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• 1980

검정곰팡이(Aspergillus niger)의 동조적(同調的) 분화(分化)를 시행(施行)하면서 acid-phenol 용성(溶性)인 세포단백질(細包蛋白質)을 추출(抽出)하여 포자형성(胞子形成) 전후(前後)의 단백상(蛋白像)의 변화(變化)를 polyacrylamide gel 전기영동법(電氣泳動法)으로 추구(追究)하였다. 결과(結果)에서 1종(種)의 단백질(蛋白質)(분자량(分子量) 약(約) 27,000 정도(程度))이 포자형성기(胞子形成期)에 신생(新生)함을 알았다. 포자(胞子)의 발아시(發芽時)에는 18개의 단백질(蛋白質)밴드가 있으며 포자형성시(胞子形成時)에는 19개가 있었다. Amido black 색소(色素)의 염색도(染色度)는 분화시기(分化時期)에 따라서 각(各) 밴드마다 각각 많이 달랐다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.729-732
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• 2000

양파의 저온저장중 부패방지를 위한 천연항균제 개발의 일환으로 저장양파로부터 부패세균을 분리한 후 이 균에 대한 식물추출물의 항균활성을 조사하였다. 양파의 저온저장중 과육으로부터 분리한 곰팡이를 MEA와 PDA에서 배양하면서 분생자병과 분생자의 형태적 특성을 조사한 결과 플라스크형의 phialide, 분생자병의 branching type이 simple type이며, conidial head가 columnar shape 가지고 있는 것으로 확인되어 Penicillium sp. ON-211으로 동정하였다. 또한 자생 식물은 채집하여 각종 유기용매로 추출한 후 항균활성을 조사하였다. 정향나무, 탱자나무의 잎은 chloroform 분액에서 높은 활성을 나타내었고, 측백나무 열매는 물분획에서 높은 활성을 나타내었으며, 연필향나무의 잎은 ethyl acetate 분획에서 비교적 높은 항균 활성이 나타났다.

• 한국식물병리학회지
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• 제14권6호
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• pp.642-645
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• 1998

A severe pink mold rot on matured melon fruits occurred under a glass tunnel cultivation in Chinju at Kyeongasngnam-do Agricultural Research and Extention Services on May of 1998. Basal portion of the fruits toward blossom end was preferably infected and colonized by the fungus. The causal fungus consistently isolated from the lesions was identified as Trichothecium roseum based on following mycological characteristics. Conidia were hyaline or brightly colored, 2-celled, ovoid or elipsoid, characteristically held together zi-zag chains. Conidiophore was long, slender, simple, septate, bearing conidia-meristem arthrospores-apically, singly when young and successively by slight growth of conidiphore apex. Optimum temperature for mycelial growth and conidial germination was recorded at 20~$25^{\circ}C$. However, over 48% of the fungal conidia were germinated at 15$^{\circ}C$ and mycelial growth was only slightly slower than $25^{\circ}C$. The temperature profiles and high humidity available during the cultivation were considered favorable for the pathogen as showing 22% infection rate on matured melon fruits cv. Saros. This is the first report of pink mold rot of melon caused by T. roseum in Korea.

• The Plant Pathology Journal
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• 제16권3호
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• pp.147-150
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• 2000

Sporulation patterns of rice blast fungus were studied at relatively later stages of leaf blast and neck blast seasons in Icheon, Korea. This experiment was done by detaching lesion-bearing leaves and panicle bases. The number of conidia remaining on the leaf blast lesions of different cultivars from Jul 20 to Jul 23 ranged from 3,640 to 82,740 spores. More conidia were observed on the adaxial surface because they were released from abaxial surface. After heading, sporulation was observed from the lesions on the flag leaves but the number of spores was less than in the late July. Detached panicle bases or uppermost internodes infected by Pyricularia grisea produced abundant amount of conidia. Among these panicle bases, 30.1 mm size lesion recorded the highest count of 244,560 spores. When we compared the sporulation amount using the KY-type spore trap, more conidia were recorded from intact lesions than from the lesions which removed conidia and conidiophore The ratio of conidia release against total sporulation ranged from 20.5%-25.0% for leaf blast and 8.2%-25.3% in the neck blast. Effective inoculum potential was also discussed.

• 한국균학회지
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• 제13권2호
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• pp.65-74
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• 1985

1. 분화과정에 따른 균체로부터 추출한 총 RNA량은 포자형성 직전에 증가함을 알았다. 2. 추출한 RNA를 가수분해하여 P.E.I. Cellulose TLC를 전개한 결과 AMP유도체와 IMP가 존재함을 확인하였다. 3. 포자형성을 전후하여 IMP와 AMP의 RNA에 대한 함량비율은 일정함을 알았다. 4. 포자형성 전기의 균체에 아데노신, 구아노신, 이노신, 크산토신을 처리한바 포자형성이 10배 이상 촉진되었다. 5. 포자형성 전기의 균체에 A. bisporus, F. velutipes, L. edodes의 열수추출액을 처리한바 포자형성이 $1.5{\sim}2$배 촉진되었다.

• 한국균학회지
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• 제26권3호통권86호
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• pp.399-402
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• 1998

Zoophthora radicans를 생물적 방제에 이용하기 위하며 각국에서는 우수 균주 탐색에 많은 노력을 하고 있지만 국내에서는 이 균이 보고된 바 없다. 본 연구자들은 이 균을 1998년 3월경 경북 성주군 쑥갓 재배 온실내의 복숭아 혹진딧물에서 발견하였다. 이에 국내에서 처음으로 Z. radicans를 보고하며, 무성세대의 형태적 특징을 기재한다. 이 균의 가장 중요한 분류학적 특징은 2차포자가 1차포자로부터 만들어진 긴 분생자병에서 형성되는 것이었다.

• 한국균학회지
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• 제47권1호
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• pp.29-34
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• 2019

A fungal isolate designated 17E029 was isolated from a soil sample in Jeju, Korea. The strain was similar to other Allantophomopsiella species in its morphological characteristics such as grey mycelia, conidiophore, and conidia sizes. The isolate produced aerial mycelia, which appeared grey on the reverse side of the media surfaces and turned black on the front side of the colonies. The conidiophores emanating from the hyphae were hyaline, grey, aseptate, branched, and $6.7{\sim}9.2{\times}1.8{\sim}2.5{\mu}m$. Conidiogenous cells were ovoid to subcylindrical, discrete, guttulate, and hyaline. Conidia were hyaline, aseptate, smooth, guttulate, oval to subcylindrical, irregular in shape, and $6.0{\sim}7.8{\times}3.0{\sim}3.4{\mu}m$. The strain was confirmed based on phylogenetic analysis of the closest related organism, A. pseudotsugae CBS 288.37, using the partial 28S, internal transcribed spacer rDNA regions, and partial RNA polymerase II second largest subunit locus (RPB2) gene sequences along with its culture characteristics. Therefore, morphological observations and phylogenetic analysis revealed that strain 17E029 is similar to the previously identified A. pseudotsugae. Hence, this species was described as A. pseudotsugae strain 17E029, which is a new record in Korea.

• 한국균학회지
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• 제29권1호
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• pp.75-78
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• 2001

2000년 경상남도농업기술원 창원화훼시험장 스타티스포장에서 흰가루병이 발생하여 병원균의 형태, 병원성, 분류학적인 특성을 조사를 하였다. 병징은 이병엽 앞뒷면과 잎자루, 줄기에 전형적인 흰가루 모양의 균총이 많이 형성되었고, 병징이 심하게 진전될 경우 잎에 황화되고 갈색 또는 암갈색으로 되며 결국 말라죽는다. 병반부에서 분생포자, 분생자병이 형성되었다. 분생포자는 분생자병의 끝에 형성되고 단상으로 원통형 또는 장타원형의 무색, 단포이다. 크기는 25.3{\sim}49.4{\times}12.3{\sim}17.2{\mu}m$이며, Fibrosin body는 없었다. 부착기는 손바닥 또는 가느다란 실모양이었다. 분생자병은 $2{\sim}4$개의 격막으로 되어있고 길이는 $65.7{\sim}124.8{\mu}m$이었다. Foot cell은 원통형이었다. 포장에서나 접종시 나타난 이병엽 병반부에서 자낭각은 관찰되지 않았다. 이상과 같은 본 병원균은 Oidium sp.에 의한 스타티스 흰가루병으로 동정되었으므로 국내에서 처음 확인된 새로운 병으로 보고한다.