• Journal of the Korean Society of Visualization
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• v.1 no.1
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• pp.28-33
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• 2003

This paper presents the visualization method in which 3-dimensional(3D) microchannel flow can be detected using a confocal scanning microscope. By soft-lithography, we fabricated various Bio-MEMS(Micro Electro-Mechanical System) devices such as a disposable microchip for a flow cytometer and a micro-mixer, which have 3D structures. Injecting aqueous fluorescent solution in the microfluidic devices, we measured the flow in a steady state by the confocal scanning microscope. At first, we explain the principle of the confocal scanning microscope. And then we show the results from 3D visualization of microscopic flow structures using the confocal scanning microscope.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.99.3-99
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• 2001

In fluorescence mode confocal scanning microscope, level of detected signal is very low. In object scanning type confocal scanning microscope, the additional optical system with objective lens and plane mirror was proposed to increase signal intensity, but there was none for beam scanning type confocal scanning microscope. We propose reflecting optical systems which improve signal intensity in beam scanning type confocal scanning microscope. We choose one of the proposed optical systems and design the optical system, i.e., select optical components and assign distances between the selected components. To design the optical system, we use finite ray tracing method and make cost function to be minimized.

• Korean Journal of Optics and Photonics
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• v.8 no.2
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• pp.165-168
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• 1997

We have constructed a scanning confocal microscope using a 780 nm semiconductor laser, an actuator of a compact disk player and a quad-detector. This device detects heights and characteristics of a surface. The laser focus was located at the surface of a sample by using the error signal obtained by a quad-dector, and the current supplied to the actuator for lens was displayed as a height. The materials of a surface were classified according to reflected total intensities and was displayed by different color in a monitor. The device has very samll dimensions of 30 mm$\times$20 mm$\times$20 mm and scan field is 1.6 mm$\times$1.6mm. We obtained two images, one using only reflected light and the other using an error signal from a quad-detector and compared these two images.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2000.11a
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• pp.51-54
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• 2000

The traditional surface measuring method using confocal principle requires much time to measure an object surface since it is a scanning tool. In this paper, the upgraded confocal microscope is introduced. It is also a scanning tool but it requires 2D-scanning while the traditional one requires 3D-scanning. It means the time for measuring is considerably reduced. In addition, the measuring system is configured to increase the efficiency of beam. He-Ne laser whose frequency is 632.8nm is used for the laser source. An example of measuring result through the upgraded confocal microscope is showed.

• Proceedings of the Optical Society of Korea Conference
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• 1996.09a
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• pp.84-84
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• 1996

• Korean Journal of Optics and Photonics
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• v.14 no.1
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• pp.80-84
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• 2003

We have constructed a scanning confocal microscope by directly oscillating an optical fiber in two different ways. Either a piezoelectric transducer or a tuning fork was used for the oscillation. Six frames of $640{\times}480$ pixel image were obtained in a second with piezoelectric oscillation and only one image of the same size was obtained in a second with tuning fork oscillation. Oscillation of optical fiber did not cause amy distortion of confocal images.

• Journal of the Korean Society for Precision Engineering
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• v.25 no.11
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• pp.52-57
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• 2008

We demonstrate the operation of an apparatus that we call the laser scanning confocal microscopy. It is valuable tool of the investigations for imaging process. We measured the thin metal structure through the SCM manufacture. Confocal microscopy offers several advantages including shallow depth of field, elimination of out-of-focus glare, and the ability to collect serial optical sections from thick specimens than conventional optical microscope. This research is manufactured of scanning confocal microscopy and after measured of metal materials structure.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.163.2-163
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• 2001

Confocal scanning microscopy (CSM) has been reported as an excellent method using the optical probe in scanning probe microscopy (SPM). Transmission or reflection confocal scanning microscopy (TCSM, RCSM) has been used in the three-dimensional reconstruction of specimen or the non-destructive measurement in vivo. The axial movement of the primary focal point having the information of specimen gives a good measurement performance with the great sensitivity. Application of the confocal theory and astigmatism to displacement measurement sensor uses the aperture as the pinhole or slit after collecting lens relating to confocal response in non-contact measurement; and astigmatic lens using four-segments detector as short-range sensor, long-range one combining the grating and rotary one hating the rotary directional grating. The aperture type can play an ...

• Journal of the Korean Society for Precision Engineering
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• v.21 no.2
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• pp.92-99
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• 2004

The errors can cause the serious loss of the performance of a precision machine system. In this paper, we proposed the method of allocating the alignment tolerances of the parts and applied this method to get the optimal tolerances of a Confocal Scanning Microscope. In general, tight tolerances are required to maintain the performance of a system, but a high cost of manufacturing and assembling is required to preserve the tight tolerances. The purpose of allocating the optimal tolerances is minimizing the cost while keeping the high performance of the system. In the optimal problem, we maximized the tolerances while maintaining the performance requirements. The Monte Carlo Method, a statistical simulation method, is used in tolerance analysis. Alignment tolerances of optical components of the confocal scanning microscope are optimized to minimize the cost and to maintain the observation performance of the microscope. We can also apply this method to the other precision machine system.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2007.11a
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• pp.483-484
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• 2007