• Journal of the Korean Society for Precision Engineering
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• v.25 no.11
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• pp.52-57
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• 2008

We demonstrate the operation of an apparatus that we call the laser scanning confocal microscopy. It is valuable tool of the investigations for imaging process. We measured the thin metal structure through the SCM manufacture. Confocal microscopy offers several advantages including shallow depth of field, elimination of out-of-focus glare, and the ability to collect serial optical sections from thick specimens than conventional optical microscope. This research is manufactured of scanning confocal microscopy and after measured of metal materials structure.

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.1
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• pp.1-12
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• 2006

The purpose of this study were to evaluate the efficacy of the newly developed Digital Imaging Fiber-Optic Trans-illumination (DIFOTI) system in detecting carious lesions in vivo as gold standard with confocal laser scanning microscopy and compared the efficacy of traditional radiography and DIFOTI system in vito as gold standard with confocal laser scanning microscopy, too. For the in vivo study, the subject pool consisted of 23 grammar school age patients just prior to entering the mixed dentition phase Each patient was given a DIFOTI examination of the anterior and posterior teeth. During $6{\sim}8$ months, the naturally expire primary teeth were collected and the efficacy of DIFOTI system was compared with confocal laser scanning microscopy. For in vitro study, 40 primary teeth were collected and decalcified by Carbopol decalcification solution for 1, 2, 4 and 8 days. Every experiment period, all teeth were DIFOTI examined and sectioned to take an image of confocal laser scanning microscopy Sensitivity and specificity were calculated from the result of DIFOTI examine and confocal laser scanning microscopy analysis. The results are as follows : 1. From the in vivo study, the sensitivity of DIFOTI examine was 0. 61 and specificity was 0.63. 2. From the in vivo study, the sensitivity of DIFOTI examine was 0.71 and specificity was 0.75.

• Archives of Plastic Surgery
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• v.42 no.5
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• pp.626-629
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• 2015

Peripheral nerve injuries remain a challenge for reconstructive surgeons with many patients obtaining suboptimal results. Understanding the level of injury is imperative for successful repair. Current methods for distinguishing healthy from damaged nerve are time consuming and possess limited efficacy. Confocal laser endomicroscopy (CLE) is an emerging optical biopsy technology that enables dynamic, high resolution, sub-surface imaging of live tissue. Porcine sciatic nerve was either left undamaged or briefly clamped to simulate injury. Diluted fluorescein was applied topically to the nerve. CLE imaging was performed by direct contact of the probe with nerve tissue. Images representative of both damaged and undamaged nerve fibers were collected and compared to routine H&E histology. Optical biopsy of undamaged nerve revealed bands of longitudinal nerve fibers, distinct from surrounding adipose and connective tissue. When damaged, these bands appear truncated and terminate in blebs of opacity. H&E staining revealed similar features in damaged nerve fibers. These results prompt development of a protocol for imaging peripheral nerves intraoperatively. To this end, improving surgeons' ability to understand the level of injury through real-time imaging will allow for faster and more informed operative decisions than the current standard permits.

• Proceedings of the KAIS Fall Conference
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• 2010.05a
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• pp.108-112
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• 2010

Confocal Imaging System is an essential machine for a wide range of inspection wafer. For concurrent and fast acquiring the image data of four channels, the new image acquisition system used the protocol of camera-link standard with the full mode of configuration in interconnection with a frame grabber integrated in a computer, which is popularly used for many cameras, so the programming environment of image processing is optional such as Visual C++, Matlab. In addition, many conventional methods were coordinately used for contribution to build the high quality of images for precise processing analog signals of PhotoMutiplier Tubes(PMTs), accurate control of scanning device, sensitivity of PMTs, and laser source. The prototype of new image acquisition system, could meet the goal of development, it is used in LSCM for high content screening to investigation the processes of elements of living specimens at the same time by simultaneous grab image data on 4 PMTs channels.

• Bulletin of the Korean Chemical Society
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• v.35 no.8
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• pp.2400-2402
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• 2014

Cu (II) detection is of great importance owing to its significant function in various biological processes. In this report, we developed a novel coumarin-based chemosensor bearing the salicylaldimine unit (2) for $Cu^{2+}$ selective detection. The results from fluorescence spectra demonstrated that the sensor could selectively recognize $Cu^{2+}$ over other metal cations and the detection limit is as low as $0.2{\mu}M$. Moreover, the confocal fluorescence imaging in HepG2 cells illustrated its potential for biological applications.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.41 no.3
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• pp.279-285
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• 2015

Hyperpigmentation on face is a highly anxiety-producing symptom, especially for women from the aspect of beauty. Pigmentation of the skin is related to the amount of melanin that provides protection against UV radiation. In vivo reflectance confocal microscopy is a non-invasive imaging tool allowing visualization of the skin without tissue alteration, by placing a microscopy directly on the living skin. The aim of this study was to develop the new evaluation method of whitening effects using in vivo reflectance confocal microscopy and to validate other instruments for measuring skin colors, and UV-induced hyperpigmentation was elicited on the inside skin of the forearm. It suggested that the new method for whitening effects using the confocal microscopy was useful to evaluate the de-pigmentation products and to easy for understanding to customers.

• Journal of Advanced Information Technology and Convergence
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• v.9 no.1
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• pp.89-102
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• 2019

Collagen can be used in building artificial skin replacements for treatment of burns and towards the reconstruction of bone as well as researching cell behavior and cellular interaction. The strength of collagen in connective tissue rests on the characteristics of collagen fibers. 3D confocal imaging of collagen fibers enables the characterization of their spatial distribution as related to their function. However, the image stacks acquired with confocal laser-scanning microscope does not clearly show the collagen architecture in 3D. Therefore, we developed a new method to reconstruct, visualize and characterize collagen fibers from fluorescence confocal images. First, we exploit the tensor voting framework to extract sparse reliable information about collagen structure in a 3D image and therefore denoise and filter the acquired image stack. We then propose to segment the collagen fibers by defining an energy term based on the Hessian matrix. This energy term is minimized by a min cut-max flow algorithm that allows adaptive regularization. We demonstrate the efficacy of our methods by visualizing reconstructed collagen from specific 3D image stack.

• Current Optics and Photonics
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• v.6 no.6
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• pp.550-564
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• 2022

Optical microscopy is a useful tool for study in the biological sciences. With an optical microscope, we can observe the micro world of life such as tissues, cells, and proteins. A fluorescent dye or a fluorescent protein provides an opportunity to mark a specific target in the crowd of biological samples, so that an image of a specific target can be observed by an optical microscope. The optical microscope, however, is constrained in resolution due to diffraction limit. Super-resolution microscopy made a breakthrough with this diffraction limit. Using a super-resolution microscope, many biomolecules are observed beyond the diffraction limit in cells. In the case of volumetric imaging, the super-resolution techniques are only applied to a limited area due to long imaging time, multiple scattering of photons, and sample-induced aberration in deep tissue. In this article, we review recent advances in super-resolution microscopy for volumetric imaging. The super-resolution techniques have been integrated with various modalities, such as a line-scan confocal microscope, a spinning disk confocal microscope, a light sheet microscope, and point spread function engineering. Super-resolution microscopy combined with adaptive optics by compensating for wave distortions is a promising method for deep tissue imaging and biomedical applications.

• Medical Lasers
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• v.9 no.1
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• pp.25-33
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• 2020

Optical-based imaging technology has high resolution and can assess images in real time. Numerous studies have been conducted for its application in the dental field. The current research introduces an oral camera that includes fluorescent imaging, a second study examining a 3D intraoral scanner applying a confocal method and a polarization structure that identifies the 3D image of a tooth, and finally, an optical coherence tomography technique. Using this technique, we introduce a new concept 3D oral scanner that simultaneously implements 3D structural imaging as well as images that diagnose the inside of teeth. With the development of light source technology and detector technology, various optical-based imaging technologies are expected to be applied in dentistry.

• Proceedings of the PSK Conference
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• 2004.10a
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• pp.109-110
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• 2004