• Journal of Microbiology and Biotechnology
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• v.21 no.5
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• pp.477-482
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• 2011

There are accumulating evidences suggesting that connexin (Cx), a gap junction channel-forming protein, acts as a growth suppressor in various cancer cells, and this effect is attributeed to the gap junction-mediated intercellular communication (GJIC). In order to characterize the relationship between the growth-arresting activity of Cx26 and its cytoplasmic localizations after expression, we linked a nuclear export signal (NES) sequence to Cx26 cDNA before transfecting into a rat breast cancer cell line. A confocal fluorescent microscopic observation revealed that the insertion of NES minimized the nuclear expression of Cx26, and increased its cytoplasmic expression, including plasma membrane junctions. Total cell counting and BrdUrd-labeling experiments showed that the growth of the breast cancer cells was inhibited by 74% upon transfection of Cx26-NES, whereas only 9% inhibition was observed with only Cx26 cDNA.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.426.3-427
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• 2002

The aim of this study was to enhance the transfection efficiency of emulsion-mediated gene expression by using chitosan, Conventional DNA/emulsion complexes and precondensed DNA/emulsion complexes were prepared by adding either naked or precondensed plasmids to cationic emulsion. The zeta potential. TEM, and size of transfection complexes were measured. In vitro transfection efficiency for boty complexes was also studied by several methods: flow cytometer, expression analysis by confocal microscope, RT-PCR, and in addition. (omitted)

• Proceedings of the Korean Society Of Semiconductor Equipment Technology
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• 2006.10a
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• pp.5-9
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• 2006

공초점 주사 현미경(Confocal Scanning Microscope, CSM)은 Bio-cell 및 특정 형태를 가지는 object의 고분해능 3차원 영상복원이 가능하여 3차원 측정장비로 주로 사용된다. 특히 LCD 패널 및 반도체 웨이퍼의 불량 검사 장비로의 활용이 가능하여 3차원 영상으로부터 불량 여부와 원인을 알아낼 수 있다. 하지만 생산 공정에서 불량 검사를 하기 위해서는 고속, 고품질의 성능이 요구된다. 따라서 이 논문에서는 공초점 주사 현미경을 이용하여 고분해능으로 고속, 고품질의 3차원 영상복원을 할 때 어떠한 조건이 요구되는지 알아보고 시뮬레이션 하도록 하겠다.

• Macromolecular Research
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• v.15 no.6
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• pp.513-519
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• 2007

Self-assembled nanoparticles have great potential to act as vehicles for hydrophobic drug delivery. Understanding nanoparticle cellular internalization is essential for designing drugs intended for intracellular delivery. Here, the endocytosis and exocytosis of fluorescein isothiocyanate (FITC)-conjugated glycol chitosan (FGC) self-assembled nanoparticles were investigated by flow cytometry and confocal microscopy. The cellular internalization of FGC nanoparticles was initiated by nonspecific interactions between nanoparticles and cell membranes. Although adsorptive endocytosis of the nanoparticles occurred quickly, significant amounts of FGC nanoparticles were exocytosed, particularly in the early stage of endocytosis. The amount of exocytosed nanoparticles was dependent on the pre-incubation time with nanoparticles, suggesting that exocytosis is dependent on the progress of endocytosis. FGC nanoparticles internalized by adsorptive endocytosis were distributed in the cytoplasm, but not in the nucleus. In vitro cell cycle analysis demonstrated that FGC nanoparticles delivered paclitaxel into the cytoplasm and were effective in arresting cancer cell growth.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.31 no.4
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• pp.8-15
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• 1999

The effects of Condebelt press drying on the densification of recycled sheets made from KOCC , AOCC UKP and BKP were examined using CLSM (Confocal Laser Scanning Microscope) and thermal image analysis techniques. It was shown that significant improvement in sheet density, compression strength, tensile strength, surface smoothness was obtained by Condebelt drying. Densification effect by press drying was most prominent for recycled KOCC sheets. And this beneficial effect of Condebelt drying of increasing sheet density and strength was shown to provide opportunities of reducing the utilization ratio of AOCC for cost saving.

• Korean Journal of Optics and Photonics
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• v.10 no.5
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• pp.369-372
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• 1999

We have constructed an interferometric microscopy using a Michelson interferometer and a He-Ne laser. The three dimensional image was obtained by the interference from the reflected signal by a sample surface and from the reflected signal by a mirror. The axial resolution obtained by Michelson interferometric microscopy is as good as that of the white-light interferometer, but the same fringe is obtained when optical path difference is half-wavelength. The image from Michelson interferometric microscopy was compared with the images from the various types of confocal microscopy.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2010.03b
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• pp.40-40
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• 2010

CdSe nanocrystals (NCs) have been decorated on singlewalled carbon nanotubes (SWCNTs) by combining a method of chemically modified substrate along with gate-bias control. CdSe/ZnS core/shell quantum dots were negatively charged by adding mercaptoacetic acid (MAA). The silicon oxide substrate was decorated by octadecyltrichlorosilane (OTS) and converted to hydrophobic surface. The negatively charged CdSe NCs were adsorbed on the SWCNT surface by applying the negative gate bias. The selective adsorption of CdSe quantum dots on SWCNTs was confirmed by confocal laser scanning microscope. The measured photocurrent clearly demonstrates that CdSe NCs decorated SWCNT can be used for photodetector and solar cell that are operable over a wide range of wavelengths.

• Journal of Industrial Technology
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• v.29 no.B
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• pp.115-119
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• 2009

Green fluorescent protein (GFPuv) was displayed on the surface of ethanol-producing bacteria Zymomonas mobilis using N-terminal domain of ice nucleation protein (INP) as an anchoring motif. To evaluate the ice nucleation protein as plausible anchor motif in Z. mobilis, GFPuv gene was subcloned into Zymomonas expression vector yielding pBBR1MCS-3/pPDC/INPN/GFPuv plasmid., INP-GFPuv fusion protein was expressed in Z. mobilis and its fluorescence was verified by confocal microscopy. The successful display of GFPuv on Zymomonas mobilis suggest that INP anchor motif could be used for future fusion partner in Z. mobilis strain improvement.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.25 no.3
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• pp.224-229
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• 2016

Recently, polymeric micro-fluidic biochips with numerous micro patterns on the surface were fabricated by injection molding for realizing low-cost mass production of devices. To evaluate the effects of process parameters on large-scale micro-structure replication, a $50{\times}50mm^2$ tool insert with surface structures having a patterns of trapezoidal shapes (height: $30{\mu}m$) was employed. During injection molding, PMMA was used; packing phase parameters and mold temperature were investigated. The replicated surface textures were quantitatively characterized by confocal laser microscopy with 10-nm resolution. The degree of replication at low mold temperatures was found to be higher than that at high mold temperature at the beginning of the packing stage. Thereafter, the degree of replication increased to a greater extent at higher mold temperatures; application of higher mold temperatures improved the degree of replication.

• Korean Journal of Bronchoesophagology
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• v.15 no.2
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• pp.30-35
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• 2009

Lung cancer could be developed through a series of morphological changes from dysplasia to carcinoma in situ and then invasive cancer. However, precancerous lesions are generally a few cell layers thick and are detected only by chance. Autofluorescence bronchoscopy(AFB) is one of the newly developed diagnostic tools to detect the pre-cancerous lesions m the bronchial tissue. Several studies have shown that AFB improved the rate of detection of cancer and dysplastic lesions of the airway, especially those in intraepithelial stage. However, there were high rates of false positive with AFB, and it is also important to develop non-biopsy methods because of lack of accurate information of variable course of preneoplastic lesions regarding progression. So, many other technologies were developed, such as narrow band imaging(NBI), endobronchoscopic ultrasound, optical coherence tomography, and confocal fluorescence microendoscopy. Among the new machines, NBI is a new optical technology that can clearly visualize the microvascular structure m the mucosal layer. NBI seems to increase specificity without compromising sensitivity. In the future such techniques would make it possible to precisely study in detail the natural history of the premalignant epithelium.