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Production of Colchicine Induced Tetraploids in Rye (Secale cereale L.) (콜히친 처리에 의한 4배체 호밀 육성)

  • Hwang, Jong-Jin;Kim, Dea-Wook;Kim, Chul-Woo;Son, Beum-Young;Baek, Seong-Beum;Park, Hyong-Ho;Ku, Ja-Hwan;Kim, Jung-Tae;Lee, Jin-Seok;Moon, Jung-Kyung;Kwon, Young-Up;Han, Ouk-Kyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.4
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    • pp.343-352
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    • 2012
  • This experiment was carried out to report some data such as survival rate, tetraploid production efficiency, and agronomic characteristics of offspring from the induced tetraploid by the colchicine treatment in rye. The colchicine was soaked with 0.05%, 12 hours in dark condition and at two growth stages (green seed and 2nd leaf stage) in diploid ryes. Flow cytometry (FC) was proved to be efficient and rapid tool for screening ploidy levels in rye, showing around 40 to 60 in DNA amount (DAP1) corresponding to diploid and 80 to 110 tetraploid. There were 18.5% of survival rate at green seed treatment and 78% at 2nd leaf stage in average of two rye cultivars, Gogu and Jogreen, but in reverse 50.9% and 1.1% in the ratio of tetraploid to total tillers among the plants survived, respectively, resulting in 9.42% of tetraploid production rate in green seed treatment and 0.86% at 2nd leaf stage, respectively. In green seed treatment, there were 33% of survival rate in Gogu, 4% in Jogreen in 1st year, but 56% in Gogu, 21% in Jogreen and 49% in Charmgreen, respectively. The rate of tetraploid to total spikes among survived was 53.7% in Gogu, 32.4% in Jogreen, and 50.9% in average in 1st year, and 64.1% in Gogu, 51.5% in Jogreen, 60% in Charmgreen, and 60.5% in average in 2nd year. In green seed treatment, tetraploid production rate (survival rate ${\times}$ tetraploid ratio ${\times}$ 100) was 17.7% in Gogu and 1.3% in Jogreen and 9.42% in average in 1st year, and 35.9% in Gogu, 10.8% in Jogreen, 29.4% in Charmgreen, and 25.4% in average of three diploid rye cultivars. By the colchicine treatment with 0.05% for 12 hours in Gogu and Jogreen, 35 tetraploid plants were obtained and they produced 2,673 seeds with 148 spikes. There were 3.3-4.4 in the number of spikes per plant, 15.6-18.3 in grain number per spike, and 37.6 g in Gogu and 46.8 g in Jogreen in the 1,000-grain weight.

Identification of Growth Inhibitory Substance on Food-borne Microorganisms from Commiphora molmol Engl. and Its Application to Food Products (몰약(Commiphora molmol Engl.)의 식중독 미생물 증식 억제 물질의 구조동정 및 식품적용)

  • Han, Ji-Sook;Shin, Dong-Hwa;Baek, Nam-In
    • Korean Journal of Food Science and Technology
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    • v.33 no.4
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    • pp.401-408
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    • 2001
  • The ethanol extract and n-hexane fraction of Commiphora molmol Engl. showed minimum inhibitory concentration of 50 ppm and 25 ppm, respectively, on 5 strains of Listeria monocytogenes at $32^{\circ}C$. The purified substance, C3-3-2 fraction, was isolated by silica gel column and preparative thin layer chromatography from n-hexane fraction of Commiphora molmol Engl. The C3-3-2 fraction showed a strong bactericidal activity on 5 strains of L. monocytogenes at the concentration of 10 ppm in tryptic soy broth medium. At that concentration, the viable count was reduced $5{\sim}6$ log cycle from initial cell number. The n-hexane fraction of Commiphora molmol Engl. showed strong growth inhibition at the concentration of 25 ppm on Bacillus cereus and Staphylococcus aureus, at 50 ppm in broth on Salmonella enteritidis, and at 500 ppm on Vibrio parahaemolyticus. The purified antimicrobial substance, the C3-3-2 fraction, was identified as m-nonylphenol by on the basis of the $^1H-,\;^{13}C-NMR$ and EI/MS data. For the application test, the C3-3-2 fraction which was purely isolated from Commiphora molmol Engl. at 100 ppm were applied to minced Alaska pollack and ground beef at $32^{\circ}C$ and $5^{\circ}C$. The antimicrobial substances did not reduce L. monocytogenes ATCC 19113 at $32^{\circ}C$, while they reduced L. monocytogenes ATCC 19113 in viable number at $5^{\circ}C$. However, the antimicrobial effect of C3-3-2 fraction in food system was lower than that of broth condition.

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Evaluation of Effective and Organ Dose Using PCXMC Program in DUKE Phantom and Added Filter for Computed Radiography System (CR 환경에서의 흉부촬영 시 Duke Phantom과 부가여과를 이용한 유효선량 및 장기선량 평가)

  • Kang, Byung-Sam;Park, Min-Joo;Kim, Seung-Chul
    • Journal of radiological science and technology
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    • v.37 no.1
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    • pp.7-14
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    • 2014
  • By using a Chest Phantom(DUKE Phantom) focusing on dose reduction of diagnostic radiation field with the most use of artificial radiation, and attempt to reduce radiation dose studies technical radiation. Publisher of the main user of the X-ray Radiological technologists, Examine the effect of reducing the radiation dose to apply additional filtering of the X-ray generator. In order to understand the organ dose and effective dose by using the PC-Based Monte Carlo Program(PCXMC) Program, the patient receives, was carried out this research. In this experiment, by applying a complex filter using a copper and Al(aluminum,13) and filtered single of using only aluminum with the condition set, and measures the number of the disk of copper indicated by DUKE Phantom. The combination of the composite filtration and filtration of a single number of the disk of the copper is the same, with the PCXMC 2.0. Program looking combination of additional filtration fewest absorbed dose was calculated effective dose and organ dose. Although depends on the use mAs, The 80 kVp AP projection conditions, it is possible to reduce the effective amount of about 84 % from about 30 % to a maximum at least. The 120 kVp PA projection conditions, it is possible to reduce the effective amount of about 71 % from about 41 % to a maximum of at least. The organ dose, dose reduction rate was different in each organ, but it showed a decrease of dose rate of 30 % to up 100 % at least. Additional filtration was used on the imaging conditions throughout the study. There was no change in terms of video quality at low doses. It was found that using the DUKE Phantom and PCXMC 2.0 Program were suitable to calculate the effect of reducing the effective dose and organ dose.

Factors Related with Job Satisfaction in Workers - Through the Application of NIOSH Job Stress Model - (직장인의 직무만족도 관련요인 분석 - NIOSH의 직무스트레스 모형을 적용하여 -)

  • Kim, Soon-Lae;Lee, Bok-Im;Lee, Jong-Eun;Rhee, Kyung-Yong;Jung, Hye-Sun
    • Research in Community and Public Health Nursing
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    • v.14 no.2
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    • pp.190-199
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    • 2003
  • This study was conducted to determine the factors affecting job satisfaction in workers by using the Job Stress Model proposed by the National Institute for Occupational Safety and Health (NIOSH). Data were collected from December 1 to December 30, 1999. The subjects were 2,133 workers employed at 155 work sites, who were examined using NIOSH Job Stress questionnaire translated by the Korea Occupational Safety ${\pounds}|$ Health Academy and Occupational Safety ${\pounds}|$ Health Research Institute. SAS/PC program was used for statistical analysis using descriptive analysis. Pearson's correlation coefficient, ANOVA, and Stepwise multiple regression analysis. The results of this study were as follows. 1. According to general characteristics of the subjects, job satisfaction was high in those with less number of children. 2. By work condition, job satisfaction was higher in those who were working in a permanent job position, were working with regular time basis than with shift basis, were working in regular shift hours than in changing shift hours, were working for a short period, and were working less hours and overtime works per week. 3. In terms of physical work environment, job satisfaction was significantly related to 10 physical environmental factors. In other words, job satisfaction was high in workers who were working in an environment with no noise, bright light, temperature adjusted to an appropriate level during summer and winter, humidity adjusted to an appropriate level. well ventilation, clean air, no exposure to hazardous substance during work hour, overall pleasant work environment and not crowded work space. 4. By work-related factors, job satisfaction was high in those with less ambiguity about future job and role, high job control/autonomy, and less workload. On the other hand, job satisfaction was low in those with little utilization of competencies, and much role conflict at work and workload. 5. As for the relationships between job satisfaction and the non-work related factors, job satisfaction was high in workers who were volunteering at different organizations or active in religious activities for 5-10 hours per week. 6. In the relationships between job satisfaction and buffering factors, significantly positive correlations were found between job satisfaction and factors such as support by direct superior, support by peers, and support by spouse, friend and family. 7. There were nine factors that affected job satisfaction in the workers: age, number of children, work hours per week, noise, temperature at the work site during summer, uncomfortable physical environment, role ambiguity, role conflict, ambiguity in job future, work load, no utilization of competencies and social support from direct supervisor. These nine factors accounted for 26% of the total variance in the multiple regression analysis. In conclusion. the following are proposed based on the results of this study. 1. The most important physical environmental factors affecting job satisfaction in workers were noise, role ambiguity, and work load, suggesting a need to develop strategies or programs to manage these factors at work sites. 2. A support system that could promote job satisfaction is needed by emphasizing the roles of occupational health nurses who may be stationed at work sites and manage the factors that could generate job stress. 3. Job satisfaction is one of the three acute responses to stress proposed in NIOSH job stress model (job satisfaction. physical discomfort and industrial accidents). Therefore, further studies need to be conducted on the other two issues.

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The Effect of Intradermal Acupuncture on The Patients suffering from Insomnia after Stroke (중풍환자(中風患者)의 불면증(不眠症)에 피내침(皮內針)의 유효성(有效性) 및 적응증(適應證) 평가(評價))

  • Lee Sang-Ho;Kim Eun-Ju;Kim Lee-Dong;Yun Sang-Pil;Lee Cha-Ro;Hong Jin-Woo;Jeong Dong-Won;Moon Sang-Kwan;Cho Ki-Ho;Kim Young-Suk
    • The Journal of Internal Korean Medicine
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    • v.25 no.1
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    • pp.138-148
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    • 2004
  • Objective : Insomnia is the disorder of initiation or maintenance in sleeping that results in daytime sleepiness and dysfunction, and it arises from multiple psychological, physiological and environmental factors. A number of stroke patients suffer from insomnia are classified as sleep disorder patients with physical illness. In addition, insomnia may have profound deleterious effects on the natural course of stroke. We are to assess the effectiveness of intradermal acupucture on stroke combined with insomnia. Methods : We recruited hospitalized patients with insomnia after stroke. Then, the subjects were allocated into a treatment group and a control group by randomization. The treatment group received intradermal acupuncture therapy at He-7(神門), EH-6(內關) correctly. However in control group, intradermal acupuncture were just attached to He-7(神門), EH-6(內關) without insertion. Treatment over time at first day and second day a various indexes were repeatedly checked such as sleep latency, total sleep time, sleep quality, condition on awakening, ability of concentration, morning sleepiness, Insomnia Severity Index, and Athens Insomnia Scale. In the second analysis, we divided the treatment group into a response and a non-response group by their response to intradermal acupuncture. With the acupuncture treatment, accompanied symptoms were checked Results : Among thirty two subjects, two of them were dropped out: One complained the pain from needle insertion, and the other underwent the change of his herbal medication. At baseline investigation with the residual 30 subjects, the control and the treatment group were assessed to have an equal comparability. In the treatment group, sleep latency, total sleeping time, a number of awakenings, sleep quality and ability of concentration showed improvement significantly compared with the control group. The non-response Group showed symptoms such as nausea and halitosis, belching and acid regurgitation, abundant expectoration, while Responder Group showed palpitation, oppressed feelings in chest and sleep disorder. Conclusions: The result of this clinical study suggests that the intradermal acupuncture at He-7(神門), EH-6(內關) is one of the effective treatments for the insomnia after stroke.

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Flow Resistance and Modeling Rule of Fishing Nets -2. Flow Resistance of Bag Nets- (그물어구의 유수저항과 모형수칙 -2. 자루형 그물의 유수저항-)

  • KIM Dae-An
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.2
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    • pp.194-201
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    • 1995
  • In order to make clear the resistance of bag nets, the resistance R of bag nets with wall area S designed in pyramid shape was measured in a circulating water tank with control of flow velocity v and the coefficient k in $R=kSv^2$ was investigated. The coefficient k showed no change In the nets designed in regular pyramid shape when their mouths were attached alternately to the circular and square frames, because their shape in water became a circular cone in the circular frame and equal to the cone with the exception of the vicinity of frame in the square one. On the other hand, a net designed in right pyramid shape and then attached to a rectangular frame showed an elliptic cone with the exception of the vicinity of frame in water, but produced no significant difference in value of k in comparison with that making a circular cone in water. In the nets making a circular cone in water, k was higher in nets with larger d/l, ratio of diameter d to length I of bars, and decreased as the ratio S/S_m$ of S to the area $S_m$ of net mouth was increased or as the attack angle 9 of net to the water flow was decreased. But the value of ks15m was almost constant in the region of S/S_m=1-4$ or $\theta=15-90^{\circ}$ and in creased linearly in S/S_m>4 or in $\theta<15^{\circ}$ However, these variation of k could be summarized by the equation obtained in the previous paper. That is, the coefficient $k(kg\;\cdot\;sec^2/m^4)$ of bag nets was expressed as $$k=160R_e\;^{-01}(\frac{S_n}{S_m})^{1.2}\;(\frac{S_m}{S})^{1.6}$$ for the condition of $R_e<100$ and $$k=100(\frac{S_n}{S_m})^{1.2}\;(\frac{S_m}{S})^{1.6}$$ for $R_e\geq100$, where $S_n$ is their total area projected to the plane perpendicular to the water flow and $R_e$ the Reynolds' number on which the representative size was taken by the value of $\lambda$ defined as $$\lambda={\frac{\pi d^2}{21\;sin\;2\varphi}$$ where If is the angle between two adjacent bars, d the diameter of bars, and 21 the mesh size. Conclusively, it is clarified that the coefficient k obtained in the previous paper agrees with the experimental results for bag nets.

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Flow Resistance and Modeling Rule of Fishing Nets 3. Establishment of Modeling Rule and its Theoritical Examination (그물어구의 유수저항과 모형수칙 3. 모형수칙의 수립 및 이론적 검토)

  • KIM Dae-An
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.4
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    • pp.543-549
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    • 1997
  • The problems in the existing modeling rules for fishing nets, especially in the Tauti's rule which had been used most commonly, were investigated and it was found that the rules could not give a good similarity between the prototype and model nets because they din neither analyze the flow resistance of nets accurately nor decide the ratio of flow velocity between the two nets properly. Thus, the modeling rule was newly derived by regarding the nets as holey structures sucking water into their mouth and then filtering water through their meshes as in the previous paper. The similarity conditions obtained, between the two nets distinguished by subscript 1 and 2, are as follows; $$\frac{d_2}{d_1}=\sqrt{\frac{l_2}{l_1}},\;\frac{N_2}{N_1}=(\frac{d_1}{d_2})^{1.5}\frac{L_2}{L_1},\;\varphi_1=\varphi_2,\;\frac{d_{r2}}{d_{r1}}=\sqrt{\frac{L_2{(\rho_{r1}-\rho_{w1})}}{{L_1{(\rho_{r2}-\rho_{w2})}}$$ $$\frac{N_{a2}}{N_{a1}}=\frac{W_{a1}}{W_{a2}}(\frac{L_2}{L_1})^2,\;\nu_1=\nu_2\;and\;\frac{R_2}{R_1}=(\frac{L_2}{L_1})^2$$, where L is the length of nettings, d the diameter of netting twines, 2l the mesh size, $2\varphi$ the angle between two adjacent bars, N the number of meshes at the sides of nettings, $d_r$, the diameter of ropes, $\rho_r$, the specific gravity of ropes, $W_a$ the weight in water of one piece of float or sinker, $N_a$ the number of floats or sinkers, $\nu$ the flow velocity, and R the flow resistance of net. In the case where the model experiments aim at investigating the influence of weight in water of nettings on their shapes in nets subjected to the water flow of very low velocity, however, the following condition is added; $$\frac{\rho_2-\rho_{w2}}{\rho_1-\rho_{w1}}=\frac{d_1}{d_2}$$ where $\rho$ is the specific gravity of netting twines.

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Four-Wheel Tractor Utilization in Korea(II) -Repair and Maintenance- (농용(農用) 트랙터 이용(利用)에 관(關)한 조사연구(調査硏究)(II) -고장(故障) 및 수리실태분석(修理實態分析)-)

  • Park, Ho Seok;Lee, Yong Kook
    • Journal of Biosystems Engineering
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    • v.6 no.2
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    • pp.65-76
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    • 1982
  • This survey was conducted to investigate the status of repair and maintenance of 4 wheel tractor for a basic reference to the improvement of quality and proper utilization of tractors. Thirty two counties from eight provinces, except Jeju, were covered in this study in order to investigate annual break-down and repair of tractor in 1980. The analyzed results are summarized as follows; 1. The average number of break-down of large size tractors(47ps) was 5.0 times in a year and it was about twice of that of small size tractors(19-23ps). The break-down frequency per 100 hours of use was 1.11 times in the large size and 0.65 times in the small size tractors. 2. 75.6 percent of total break-down was occured in main body of tractor and 24.4 percent in attachments. In particular, the break-down of plow and rotavator was more than 80 percent of total break-down of the small size tractor attachments. 3. The large size tractors which were occured more than one times of break-down a year was 75 percent and its rate of the small size tractor was 62 percent. But 9 percent of tractor surveyed had more than ten times of break-down in a year 4. The frequency of break-down had a peak in May, and it was directly proportional to the hours of use. 5. The causes of break-down were poor maintenance and operation by 29.8 precent, old parts by 30.2 percent, poor quality of parts by 20.6 percent, poor field condition by 16.3 percent and others by 3.1 percent. 6. Annual number of repair was 5.5 times and among them 55.6 percent was done by shop and 44.4 percent by operator. 7. Total required repair time was 30.6 hours a year in the large size tractor and 19.9 hours in the small size tractor. Average repair time was 3.62 hours a time. 8. Annual repair cost was 278 thousand won in the large size tractor and 70 thousand won in the small size tractor. The repair cost per hour of use was 621 won in the large size and 198 won in the small size tractor. 9. The repair cost rate of tractor(Y) was regulated with tractor age (X) as follow; Y=0.752X In case of the service life of tractor was 10 years, the total repair cost rate was 64 percent.

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Composting of Livestock Waste and Development of Operating Parameters I. Development of Optimum Process Parameters in Cow Manure Composting (축산 폐기물의 퇴비화 및 운용지표 개발 I. 우분의 퇴비화에 있어서 최적 공정운용지표의 개발)

  • Chung, Jae-Chun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.1 no.1
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    • pp.69-84
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    • 1993
  • In order to determine the optimum operational paramsters in cow manure composting, 4 laboratory scale composters were established. The cow manure was mixed with certain amount of saw dust to adjust the initial C/N ratio to 24, initial pH to 6.9 and composting was performed with varying operational conditions. It was found that the optimum aeration rate was 1000 ml/min kg. VS, the optimum moisture content 50% and no significant difference was found with different initial pH condition. Microorganisms were counted under the optimum conditions determined in this study. At the end of the experimental period, the number of bacteria, actinomycetes and fungi was $1.5{\times}10^9$ cells, $1.1{\times}10^8$ cells and $3.0{\times}10^8$ cells/g dry compost, respectively. At day 0, the number of coliforms, fecal coliforms and fecal streptococci was $3.1{\times}10^3$ cells, $7.5{\times}10^2$ cells and $5.6{\times}103$ cells/g dry composting material, respectively. Their population was decreased with time lapse, However, their survival time was longer than those reported by other researchers. Microorganisms were identified at the end of the experiment. Genus Bacillus was the most dominant comprising 89.3% of the total population. Among the Genus Bacillus, B. circulans compoex was the most abundant, followed by B. Stearothermophilus, B. Sphericus, B. licheniformis and B, brevis.

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Studies on the X-organ of eyestalk and the photoperiod for the control of gonadal maturation in a freshwater prawn, Macrobrachium nipponense(De Haan) (징거미새우, Macrobrachium nipponense(De Haan)의 생식소성숙 제어에 미치는 광주기와 안병의 X-organ에 관한 연구)

  • HAM Chang-Hee;KIM Dae-Jung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.26 no.1
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    • pp.76-90
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    • 1993
  • To clarify the relations between photoperiodism and x-organ of eyestalks to the control of gonadal maturation in a freshwater prawn, Macrobrachium nipponense, the present investigation was performed with following examinations: 1) the influence of water temperature and daylength on the control of gonadal maturation, 2) the effect of eyestalk ablation on the gonadal maturation, 3) the seasonal variation of histology of MEX-organ (medulla externa X-organ) in the eyestalk. In previtellogenesis period (December${\sim}$March) and vitellogenesis period (April${\sim}$May), gonadal maturation was considerablely influnced by water temperature. In these periods, GSI increased and gonads were matured with water temperature rising without regard to photoperiod conditions (12L/12D and 15L/9D). In spawning period (June${\sim}$August), however, gonadal maturation was influenced by photoperiod condition. While high value of GSI was kept at long photoperiod regime (15L/9D), GSI was decreased at short photoperiod regime (12L/12D). In resting period (September${\sim}$November), no rematuration was occurred at all the experimental regimes combinated with water temperatures ($16^{\circ}C,\;22^{\circ}C,\;28^{\circ}C$) and photoperiod (12L/12D, 15L/9D). Effect of X-organ which inhibite the gonadal maturation was stronger in resting period than that in previtellogenesis and ealy spawning periods by observations on the effectiveness of eyestalk ablation on the gonadal maturation. In MEX-organ of eyestalk, the number of neurosecretory cells of which size was over $20{\mu}m$ in diameter varied according to the reproductive cycle. The number of cells increased $77{\pm}12$ in resting period, and decreased $55{\pm}7$ in vitellogenesis period. Volume of Bellonci's organ, however, increased in vitellogenesis period in comparison with that in resting period.

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