• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.964-969
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• 2006

The efficacy of garlic extract and $PGF_2{\alpha}$ in the treatment of endometritis in cows was evaluated. A total of 26 parous cows affected with endometritis were randomly allocated into three groups. Group I (n = 10) animals were infused (IU) with 10 ml garlic extract mixed with 90 ml normal saline, three times at 12 h interval starting from the day of estrum, whereas the animals of Group II (n = 10) were treated with a single injection of $PGF_2{\alpha}$ (25 mg Lutalyse) on the $10^{th}$ to $12^{th}$ day after estrus, and group III (n = 6) remained as control. Cervico-vaginal mucus (CVM) was collected from each animal at pre- and post-treatment estrus and subjected to white side test, pH determination and total bacterial load. The clinical recovery of cows was assessed by negative white side test reaction, pH value and total bacterial count of CVM at subsequent estrus. The recovered animals were inseminated with frozen-thawed semen twice at 12 h intervals and pregnancy was confirmed at 45-60 days following insemination. A significant decline (p<0.05) in pH of CVM was observed in both the treatment groups at subsequent estrus. After treatment there was a significant (p<0.05) reduction in bacterial load, whereas, it was increased in control group. A total number of 65 isolates were identified in CVM samples comprising mostly of facultative anaerobic bacteria. Plasma $T_4$ and $T_3$ concentrations were increased in all the treated animals, whereas, a decline was observed in cortisol levels following treatment. The overall conception rate was 50% in treated groups as compared to nil pregnancy in the control.

• 농업과학연구
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• 제34권1호
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• pp.61-65
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• 2007

본 연구에서는 유산양에서 비번식계절과 번식계절에 각각 발정동기화를 유도하여 인공수정을 1차와 2차에 걸쳐 실시한 후 분만율을 조사하였다. 자아넨 유산양 암컷을 대상으로 5월과 6월 사이에 CIDR-S를 이용하여 발정을 유도하였고, 액상정액과 동결정액을 이용하여 인공수정을 실시하였다. CIDR-S를 이용한 발정유도시험에서는 유산양의 발정동기화가 90%이상을 기록하여 아주 양호한 것으로 나타났다. 그러나 분만 성적은 1차 인공수정에 의해서는 25%, 2차 인공수정에 의해서는 20%로 낮하게 나타났으며, 자연종부에 의해서는 100%의 분만율을 기록하였다. 본 연구의 결과 비번식계절에서 번식주기를 인위적으로 조절할 수 있는 가능성과 정액을 주입하는 적정한 시기를 예측할 수 있는 점을 시사해 주었으나 인공수정 방법과 정액의 저장방법에 대한 연구가 필요한 것으로 나타났다.

• 대한수의학회지
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• 제14권2호
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• pp.253-268
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• 1974

A total of 331 dairy cattle in Jeonnam area were examined for the breeding status and hematological values during the period from June to August. 1971 and 1974. The data obtained were analysed according to the status of breeding and the type of farm management. The results obtained in this work were summarized as follows: 1. 331 dairy cows were grouped as pregnant (63.81%) anestrus after parturition (12.45%), pregnancy unknown (11.48%), repeat breeder (10.32%), and others (1.94%). 2. The summery of reproductive histories and clinical examination were as follows. Average of calving interval was 16.5 months, interval from parturition to first breeding 97 days and postpartum interval to first estrus 72 days. Services per conception was 1.6 rate of postpartum estrus (60 days) 12.0%, and the rate of repent breeder 10.3%. 3. Generally, the blood values of RBC, Hb, serum total protein and A/G ratio were lower than those normal values, especially, the cows which showed abnormal values belonged to the repeat breeder and the unknown to conception group. The mean value for serum inorganic phosphorus was the normal value or hyperphosphatemia, on the other hand, the mean value for serum calcium of the repeat breeder group was the lowest than the other group. 4. Follow-up evaluations on the results of the laboratory tests strongly suggest that the problems of repeat breeder had a tendency to occur more frequently in the large herd (A and B type farm), and the Ca/P ratio of almost all the cows showed abnormal values.

• 대한화학회지
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• 제53권1호
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• pp.51-61
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• 2009

본 연구의 목적은 고등학교 ‘과학’과 ‘화학 II’의 ‘반응 속도의 측정’ 단원에서의 주된 학습 내용 을 분석하고, 학습 내용에 적절한 개선된 탐구 실험을 제시하는 것이다. 학습 내용의 분석을 위해 고등학 교 ‘과학’ 11종, ‘화학 II’ 8종의 교과서와 교사용 지도서 11종을 분석하고, 교사들의 인식을 조사하였다. 대부분의 교과서와 교사들이 ‘시간에 따라 반응 속도가 점점 느려진다’는 개념을 학습 내용으로 제시 또 는 인식하고 있으나, 교과서에 제시된 탐구 활동의 내용은 ‘시간에 따른 반응 속도의 감소’를 설명하기 에 부적절한 경우가 있었다. 학습 내용에 적절한 탐구 활동을 제안하기 위해 개선된 조건에서 염산과 마 그네슘의 반응 실험을 실시하였고, 얻어진 실험 결과를 이론적으로 예측한 결과와 비교함으로써 ‘시간 에 따른 반응 속도의 감소’를 설명하기에 타당함을 알 수 있었다. 본 연구에서 개선된 탐구 활동은 교과 서의 화학 반응 속도에 대한 개념을 보다 정확히 기술하는데 도움이 될 수 있을 것으로 기대된다.

• 한국가축번식학회지
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• 제18권1호
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• pp.55-62
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• 1994

This study was carried out to test the effects of fluorescein diacetate(FDA 0${mu}ell$/ml, 0.5${mu}ell$/ml, 5${mu}ell$/ml, 10${mu}ell$/ml or 0${mu}ell$/ml, 5${mu}ell$/ml in PBS) treated before culture on the survival of vitrified mouse morulae in vitrification solution(20% glycerol＋glycerol＋10% ethylene glycol＋30% ficoll＋10% sucrose). The results were summarized as follows; 1. The survival rate of FDA-tested fresh mouse morulae after 24 hours culture was over 96%((P4.8) in the control or treatment groups with various levels of FDA. Because the rate of mouse morulae which developed to hatched blastocysts was higher with the various levels of FDA treatment(67%) than control(50%), it was considered that toxicity of FDA did not affect the survival of mouse morulae. 2. When mouse morulae were FDA-tested in FDA 0(control), 0.5, 5, and 10${mu}ell$/ml treatment after vitrification, the development rate to expanded blastocyst were 66, 82, 64 and 76%, and FDA scores were P4.2(84%), P4.7(94%), P4.2(84%) and P3.9(78%), respectively. There were no significant differences between control and FDA treatments, but there were significant difference between 0.5${mu}ell$/ml)94%) and 10${mu}ell$/ml(78%) treatment(P<0.01). 3. The survival rates of cultured mouse morulae according to FDA-scores(P0=non-fluorescence; P1~P5=according to their fluorescence) after vitrification were P5;92%, P4;67%, P3;42% and P2.P0;0%, respectively. 4. Implantation rates of morulae stage embryos cultured into early blastocysts and implanted into uterine hornes vitrification were 14 and 11% embryos treated control(0${mu}ell$/ml) and FDA 5${mu}ell$/ml and the normal fetus development was 2% embryos for both treatments. Results of this percent study indicated that toxicity of FDA does not affect not only the survival of fresh and vitrified mouse morulae but also the development rate and implantation of fetus after transfer as well. The development rates of mouse morulae with the FDA score of P5, P4 and P3 were 92, 67 and 42%, respectively, it was considered that FDA-test was fit for the judge of survival.

• Reproductive and Developmental Biology
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• 제34권3호
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• pp.247-252
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• 2010

This study was carried out between 2008 and 2009 in four dairy farms to investigate the effect of feeding of whole crop barley silage on the reproductive performance of Holstein heifers. Two diets, mixed hay or whole crop barley silage separately from concentrate were fed 6-month old Holstein heifers (=37). In control group (=CON), heifers (n=16) were fed 6 kg (/head) mixed hay and 4 kg (/head) commercial diet. In whole crop barley silage group (=WBS), heifers (n=21) were fed 10 kg (/head) whole crop barley silage, 4 kg (/head) mixed hay and 2 kg (/head) commercial diet. To manage body weight gain, the body condition score of heifers were measured every month. The results obtained were as follows: 1. Body weight in CON and WBS heifers at 13-, 14-, 15- and 17-month old were $340{\pm}17.9$ and $342{\pm}13.6\;kg$, $356{\pm}15.7$ and $366{\pm}14.7\;kg$, $382{\pm}13.1$ and $387{\pm}14.4\;kg$, and $429{\pm}15.0$ and $417{\pm}10.3\;kg$, respectively. 2. Body condition score in CON and WBS heifers at 9-, 12-, 15- and 17-month old were $2.88{\pm}0.04$ and $2.80{\pm}0.04$, $2.88{\pm}0.04$ and $2.80{\pm}0.04$, $2.89{\pm}0.08$ and $3.00{\pm}0.07$, and $2.89{\pm}0.08$ and $3.00{\pm}0.07$, respectively. 3. Average age of sexual maturity in CON and WBS heifers were $437.3{\pm}9.9$days and $939.6{\pm}12.5$days, WBS group heifers were significantly shorter (p<0.05) than CON group heifers. 4. First-service conception rates in CON or WBS group were 81.3% (13/16) and 66.7% (14121), respectively, and cumulative conception rate to 2nd artificial insemination were 87.5% for CON and 85.7% for WBS group. Conception rate was not different between treatments.

• Reproductive and Developmental Biology
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• 제30권3호
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• pp.195-199
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• 2006

본 연구는 후산정체 젖소에 있어서 Lipopolysaccharide(LPS)의 처리가 번식 성적에 미치는 영향을 구명하기 위하여 분만 후 12시간이 경과하여도 후산이 배출되지 않은 홀스타인 개체 33두 및 대조구 12두에 대하여 분만후 20일째에 LPS 100 ${\mu}g$을 자궁 내에 주입한 다음 7일후에 발정이 발현되었을 때 인공수정을 실시하여 다음과 같은 결과를 얻었다. 후산정체 젖소에 있어서 첫 수정에 의한 수태율은 대조구의 경우 공시한 12두가 모두 첫 수정에 의해 수태가 되지 않았고 처리구는 33두중 11두가 수태되어 33.3%의 수태율을 나타내었다. 후산정체 젖소에 있어서 대조구 및 처리구의 분만후 수태까지의 일수는 각각 $149.6{\pm}34.3$일 및 $53.0{\pm}12.5$일로 처리구가 대조구에 비하여 유의적(p<0.01)으로 단축되는 경향을 나타내었다. 후산정체 젖소에 있어서 대조구 및 처리구의 수태당 종부 횟수는 각각 $3.6{\pm}0.8$회 및 $2.1{\pm}0.3$회로 처리구가 대조구에 비하여 유의적(p<0.05)으로 낮은 경향을 나타내었다.

• Reproductive and Developmental Biology
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• 제30권3호
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• pp.213-218
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• 2006

본 연구는 젖소의 분만 후 난소 주기 재개의 이상이 번식 성적에 미치는 영향을 구명하기 위해 수행하였다. 정상적인 난소 주기가 진행이 되면 배란된 날에 난소 주기가 재개된 것으로 간주하였다. 공시우의 58.8%(l14/194)가 정상적인 난소 주기의 재개를 하였고 41.2%의 소에서 난소 주기의 재개가 지연되었다. 배란 후 황체기가 20일 이상 유지되는 난소 주기의 재개지연 I형(17.5%)과 분만 후 40일 이전에 첫 배란이 나타나지 않는 난소 주기의 재개지연 II형(22.7%)이 난소 주기 재개 지연의 일반적인 형태였다. 정상적인 난소 주기를 가진 개체와 비교해 보면, 황체기가 연장된 소들의 분만 후 100일 이내의 인공수정 공시율, 수태율과 임신율은 더 낮고(각각 84.2 대 40.0%; p<0.01, 24.0 대 21.4%, 20.2 대 11.1%) 분만 후 첫 수정 일수와 공태 일수가 더 길다(각각 $64.7{\pm}2.79$ 대 $105.7{\pm}7.48$일; p<0.01, $105.1{\pm}7.16$ 대 $133.7{\pm}11.17$일). 정상우와 무배란우를 비교해 보면, 100일 이내의 수태율과 임신율이 낮고(각각 24.0대 20.0%, 20.2 대 16.3%) 분만 후 첫 수정 일수과 공태 일수가 더 길다(각각 $64.6{\pm}2.79$ 대 $72.6{\pm}4.45$일, $105.1{\pm}7.16$ 대 $120.8{\pm}12.33$일). 결론적으로 젖소의 분만 후 비정상적인 난소 주기는 인공수정 공시율, 임신율, 첫 수정 일수와 공태 기간을 포함하는 번식 성적의 저하를 유발시킨다.

• 한국안전학회지
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• 제6권3호
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• pp.21-26
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• 1991

In this study, a crack closure behavior of Al-alloy 5052-H38 was investigated. The fatigue test was performed by the four-point bending test under the contast amplitude load. Stress ratio R was changed from-1.0 to 0.5. To mcasure the fatigue crack opening point, the graph of load vs. subtracted displacement by computer program was obtained from the X - Y plotter. In order to gain the displacement data, a strain gage was attached at the back surface against the notched side. Uslng the conception of crack closure and influence of on crack growth rate a model for crack growth rate is developed for Al-alloy 5052-H38.

• 혜화의학회지
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• 제8권2호
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• pp.289-298
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• 2000

The result were obtained as follow: 1. Bladder Meridian has been used most frequently and next to Kidney Meridian has been used many time at the rate of acupuncture points. 2. Bladder Meridian has been used most frequently and next to Stomach Meridian, Kidney Meridian, Conception vessel have been used many time at the rate of frequency of used acupuncture points. 3. The acupuncture points at Lower extremity has been used most frequently and next to the point at back, chest and abdomen, upper extremity, head have been used many time. 4. The point of Ji Gu has been used most frequently and next to Dae Jang Su, Jock Sam Li, Cheon Chu, Jo Hae have been used many time. 5. In herb-medication, Seung Gi Tang has been used most frequently and next to Bi Yack Hwan, Ma In Hwan have been used many time.