• Title/Summary/Keyword: concentration measuring test

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Comparison of biological and chemical assays for measuring the concentration of residual antibiotics after treatment with gamma irradiation

  • Nam, Ji-Hyun;Shin, Ji-Hye;Kim, Tae-Hun;Yu, Seungho;Lee, Dong-Hun
    • Environmental Engineering Research
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    • v.25 no.4
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    • pp.614-621
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    • 2020
  • Antibiotic pollution is one of the factors contributing to the spread of antibiotic-resistant bacteria in the environment. Advanced oxidation and irradiation processes have been introduced to eliminate antibiotics from water and wastewater. However, few studies have reported the toxic effects of residual antibiotics and their byproducts induced by a treatment system. In this study, we compared the efficacies of chemical (high-performance liquid chromatography (HPLC)) and biological (antimicrobial susceptibility test) assays for measuring the concentrations of residual antibiotics after gamma irradiation for degrading amoxicillin, cephradine, lincomycin, and tetracycline. The concentrations of residual antibiotics estimated using the two assay methods were almost identical, except cephradine. In the case of cephradine, inhibited bacterial growth was observed that was equivalent to twice the concentration measured by HPLC in the samples subjected to gamma irradiation. The observed inhibition of bacterial growth suggested the generation of potentially toxic intermediates following antibiotic degradation. These results indicate that biological and chemical assays should be used in concert for monitoring antibiotic contamination and the toxic derivatives of antibiotic degradation. The results demonstrate that these four antibiotics can be decomposed by 2.0 kGy gamma-irradiation without toxic effects of their byproducts.

Calculation of the Surface Chloride and Estimation for the Soundness of Embedded Rebar by Using Colorimetric Distinction Method (비색판별법을 이용한 콘크리트의 표면염화물량 산정 및 매립철근의 건전도 평가)

  • Lee, Mun-Hwan;Lee, Jin-Woo
    • Journal of the Korea Concrete Institute
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    • v.15 no.6
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    • pp.794-801
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    • 2003
  • As it is important to measure the degree of the deterioration and predict service life caused by chloride in concrete structure the methods of measuring chloride in the concrete is raised important problems. This study is to set a new standard for using of the colorimetric method through grasping the character of the colorimetric distinction method, and measuring the chloride content at the place discolored. Also, to predict chloride content around embeded bar and time reaching limit chloride concentration through measuring the chloride content of concrete surface by colorimetric distinction method and this study presents the new concept of concrete degradation and diagnosis of the durability by salt damage. According the results, it is possible to use colorimetric distinction method as simplified measurement to measure the fixed quantity of the chloride concentration. What is more, it would make calculation of concrete surface chloride had a wide fluctuation at the general environment extended. Also, it would be make estimating durability of reinforced concrete structures applied to the basic data.

A Compact Optical System using LED and CMOS Image Sensor for Liver Function Analysis (LED와 CMOS 이미지 센서 기반 간 기능 분석용 소형 광학장치)

  • Kim, Chul;Lim, Chang-Jin;Nam, Myung-Hyun;Kim, Dong-Sik;Seo, Sung-Kyu;Pak, Jung-Ho
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.61 no.2
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    • pp.270-275
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    • 2012
  • This paper presents a portable and compact optical device which can conveniently be used to perform a functional analysis of human liver function. The proposed system employed red/green LEDs, as a light source, and CMOS image sensor, which is commonly used in cellular phones. With this system, several blood serum samples have been evaluated for liver functional analysis by measuring light absorption level through the blood serum samples depending on aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin concentration. The light absorption through the blood serum samples containing AST, ALT, or total bilirubin can provide their concentrations. The green light absorption is more sensitive to the concentration of AST or ALT, and the red light absorption is more sensitive to the total bilirubuin concentration. Additional calibration steps were performed by using a MATLAB program in order to eliminate the light scattering effects from the extraneous particles existing in each blood serum sample. From the blind test, three standard light intensity curves through each enzyme have been obtained and the enzyme concentration values have been compared to those obtained from a commercially available biochemistry analyzer (Toshiba 200 FR). The average percent difference in the obtained concentrations from two systems for AST, ALT, and total bilirubin concentration came out to be 7.79%, 7.98%. and 7.56%, respectively, with the adjusted coefficient of determination (R2) higher than 0.98. This system can possibly lead to a low-cost and simple system that can be used as a point-of-care (POC) system in a condition without advanced equipments.

The Effect of Flow Patterns with Polymer Additivies From Two Phase Flow at Vertical up Ward in Circular Tube (원관내 수직상향 2상유동에서 고분자물질이 유동양식에 미치는 영향)

  • 김재근
    • Journal of Advanced Marine Engineering and Technology
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    • v.22 no.4
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    • pp.505-514
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    • 1998
  • Flow pattern of air-water two phase flow depends on the conditions of pressure void fraction and channel geometry. We classify the flow pattern by measuring the output signal of the conductivity probe. under the classified flow pattern we mount a visualization equipment on the test section and take pictures. We vary the concentration of pure solvent and polymer to measure local void fraction. We know that the maximum point position of local void fraction distribution move from the center of the pipe to the wall of the pipe as JSL increase when JSA is constant in two phase flow. But we find that the maximum point position of local void friction move from the wal of the pipe to the center of the pipe when polymer concentration increase.

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A STUDY OF HYDRAULIC PROPERTIES IN A SINGLE FRACTURE WITH IN-PLANE HETEROGENEITY: AN EVALUATION USING OPTICAL MEASUREMENTS OF A TRANSPARENT REPLICA

  • Sawada, Atsushi;Sato, Hisashi
    • Nuclear Engineering and Technology
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    • v.42 no.1
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    • pp.9-16
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    • 2010
  • Experimental examinations for evaluating fracutres were conducted by using transparent replicas of a single fracture in order to obtain the fracture data to contribute to the methodlogy on how to improve the definitaion of representative parameter values used for a parallel plate fracture model. Quantitative aperture distribution and quantitative tracer concentration data at each point in time were obtained by measuring the attenuation of transmitted light through the fracture in high spatial resolution. the representative aperture values evaluated from the multiple different measurement methods, such as arithmetic mean of aperture distribution measured by the optical method, transport aperture evaluated from the tracer test, and average aperture evaluated from the fracture void volume measurement converged to a unique value that indicates the accuracy of this experimental study. The aperture data was employed for verifying the numerical simulation under the assuption of Local Cubic Law and showed that the calculated flow rate through the fracture is 10%-100% larger than hydraulic test results. The quantitative tracer concentration data is also very valuable for validating existing numerical code for advection dispersion transport in-plane heterogeneous fractures.

Evaluation of a New Workplace Protection Factor―Measuring Method for Filtering Facepiece Respirator

  • Sun, Chenchen;Thelen, Christoph;Sanz, Iris Sancho;Wittmann, Andreas
    • Safety and Health at Work
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    • v.11 no.1
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    • pp.61-70
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    • 2020
  • Background: This study aims to assess whether the TSI PortaCount (Model 8020) is a measuring instrument comparable with the flame photometer. This would provide an indication for the suitability of the PortaCount for determining the workplace protection factor for particulate filtering facepiece respirators. Methods: The PortaCount (with and without the N95-CompanionTM) was compared with a stationary flame photometer from Moores (Wallisdown) Ltd (Type 1100), which is a measuring instrument used in the procedure for determining the total inward leakage of the particulate filtering facepiece respirator in the European Standard. Penetration levels of sodium chloride aerosol through sample respirators of two brands (A and B) were determined by the two measuring systems under laboratory conditions. For each brand, thirty-six measurements were conducted. The samples were split into groups according to their protection level, conditioning before testing, and aerosol concentration. The relationship between the gauged data from two measuring systems was determined. In addition, the particle size distribution inside the respirator and outside the respirator was documented. Linear regression analysis was used to calculate the association between the PortaCount (with and without the N95-CompanionTM) and the flame photometer. Results: A linear relationship was found between the raw data scaled with the PortaCount (without N95-CompanionTM) and the data detected by the flame photometer (R2 = 0.9704) under all test conditions. The distribution of particle size was found to be the same inside and outside the respirator in almost all cases. Conclusion: Based on the obtained data, the PortaCount may be applicable for the determination of workplace protection factor.

Analysis Methods for Measurement of Ammonia Concentration (가스상 암모니아 측정을 위한 분석방법별 특성 연구)

  • Sa, Jae-Hwan;Yoon, Seok-Kyung;Roh, Gi-Hwan;Jeon, Eui-Chan
    • Journal of Korean Society for Atmospheric Environment
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    • v.24 no.1
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    • pp.43-54
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    • 2008
  • Management and control of ammonia at the sources and ambient largely depend on sampling and measurement techniques. Good sampling and measurement techniques provide high quality data. The main purpose of the study is compare the analytical characteristics of the Indolphenol method which is one of the standard method in Korea with automatic analyzers for continued measuring gaseous ammonia. For comparison with other analytical methods, the verification test was designed to evaluate performance parameters; linearity, absorption efficiency, reproducibility and repeatability test, accuracy, and response time test. $R^2$ of calibration curve using IPM and CLM was very high (value is 1.000), but for EcSM $R^2$ value was estimated to be lower than IPM and CLM (as 0.991). The RSD of the CLM ranged from 0.1 to 2.3% over the nine concentration levels measured, %Ds was 0.1 to 10.7%, and average RA over all the measurements was 3.3%. The RSD of IPM and EcSM was ranged from 1.0 to 8.1, 3.9 to 14.0 respectively, and average RA were 8.71, 4.9% respectively. Rise in response times of EcSM was estimated to be 1 minute. It is found to be more sensitive than response time (which ranged from 2 to 9 minute) of CLM. For ammonia concentration measured using the IPM and the CLM from the same ammonia source, linear regression of IPM versus CLM show a slope of 0.805, an intercept of 637 ppb, and $R^2$ of 0.868.

Studies on Physicochemical Properties of Erythritol, Substitude Sugar (대체감미료 에리스리톨의 이화학적인 성질에 관한 연구)

  • Byun, Sang-Hee;Lee, Cherl-Ho
    • Korean Journal of Food Science and Technology
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    • v.29 no.6
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    • pp.1089-1093
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    • 1997
  • The physicochemical properties of erythritol were examined by measuring water absorption, solubility, water activity, heat stability, and viscosity compared to those of sucrose, xylitol, sorbitol and fructo-oligosaccharide. Erythritol showed the lowest water absorption and the highest water activity reducing capacity. In the solubility test of sweeteners, the saturation concentration of erythritol at $20^{\circ}C$ was 35.8%, which was the lowest solubility. Caramelization test and Maillard reaction test showed that erythritol was stabler than sucrose in heat treatment, while fructo-oligosaccharide showed the strongest reaction. The viscosity of erythritol was similar to that of other sweeteners at the same concentration (10%, 30% w/w). The viscosity of sweeteners increased exponentially with increasing concentration but decreased with increasing temperature following Arrhenius equation. The activation energy for flow of 30% erythritol solution was estimated to be 10.8 kcal/g mol.

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Growth Optimization of Delftia sp. for the Odor Control of Organic Waste (유기성 폐기물의 발생 악취 제거를 위한 Delftia sp.의 성장조건 최적화)

  • Kwon, Hyuk-Ku;Jung, Joon-Oh;Chu, Duk-Sung;Lee, Jang-Hoon
    • Journal of Environmental Health Sciences
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    • v.35 no.5
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    • pp.393-401
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    • 2009
  • We isolated and identified a microorganism which was excellent for ammonia oxidation in the biological control of ammonia gas in odor producing materials from organic composting. The isolated strain was tested for growth characteristics and ammonia elimination efficiency under various conditions of temperature, pH, carbon concentration and ammonia concentration. The strain was isolated from a culture broth used in a $NO_2$ producing test with Griess-Ilosvay reagent. The results of 16S rRNA sequence from the isolated strain by using BLANST (Basic Local Alignment Search Tool) and confirming RDP (Ribosomal Database Project II) and ERRD (The European Ribosomal RNA Database) indicate that the strain is related to Delftia sp. UV-Spectrophotometer (Shimadzu, UVmini-1240) was used as a microbial growth test by measuring turbidity on OD660nm and ammonia concentration was measured by Spectrophotometer (HACH, DR-4000). The optimum growth culture conditions of the ammonia oxidizer Delftia sp. were $30^{\circ}C$, pH 7, glucose concentration 1.00% and $(NH_4)_2SO_4$ 0.5 g/l. Ammonia elimination efficiency was over 94% under the same conditions.

A Study on Measuring of Fire Extinguishing Concentration for Organic Material Using Novec (노벡을 이용한 유기물의 소화농도 측정에 관한 연구)

  • Lim, Woo-Sub;SaKong, Seong-Ho;Jung, Jong-Jin;Nam, Dong-Gun;Choi, Kenu-Joo;An, Sang-Su;Kim, Jong-Won
    • Proceedings of the Korea Institute of Fire Science and Engineering Conference
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    • 2008.11a
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    • pp.456-460
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    • 2008
  • This study was on basic step to develop a good clean extinguishing agent. In order to get a satisfactory result, we tested fire extinguishing ability using Novec which is inert gas. In study used to Cup Burner Test its made by international standard ISO-14520 regulations of gaseous extinguishing agent ability test. The finding is that a new clean agent, Novec has a very efficient extinguishing ability in a state of gas.

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