• 약학회지
• /
• 제48권1호
• /
• pp.65-69
• /
• 2004

The chromatographic separation of MeOH extract of the aerial parts of Aster glehni (Compositae) led to the isolation of three sesquiterpenes, two sterols and four terpenes. Their structures were determined to be $\beta$-amyrin acetate (1), phytol (2), alismol (3), $\alpha$-tocopheryl quinone (4), $\alpha$-spinasterol (5), 10-O-methyl alismoxide (6), alismoxide (7), and 3-O-(6'-O-palmitoyl-$\beta$-D-glucosyl)-spinasta 7,22-diene (8) by physicochemical and spectroscopic methods . These compounds (1-8) were first isolated from the Aster glehni.

• 약학회지
• /
• 제47권1호
• /
• pp.10-13
• /
• 2003

The phytochemical study of Cacalia koraiensis Nakai (Compositae) led to the isolation of eight compounds, $\beta$-sitosterol (1), stigmasterol (2), phytol (3), $\beta$-sitosterol-3-Ο-glucopyranoside (4), adenostylol (5), campesterol (6), germacradien-4$\alpha$-ol (7), and quercetin-3-rhamnoside (8). Their structures were established by chemical and spectroscopic methods. The cytotoxicity of the isolated compounds was evaluated by SRB assay against five cultured human tumor cell lines.

• 한국응용곤충학회지
• /
• 제31권4호
• /
• pp.509-515
• /
• 1992

한국산 국화과(초롱꽃목) 잡초를 가해하는 곤충조사 결과, 12속 16종의 잡초에서 5목 17과 43속 45종의 곤충이 채집되었다. 그 중 노린재목과 딱정벌레목의 각각 13종으로 가장 많이 채집되었으며, 중요한 잡초가해 곤충은 진딧물과 잎벌레였다. 식물별로는 참쑥에서 15속 16종의 가장 많은 곤충이 채집되었으며 그외 엉겅퀴와 망초에서도 많은 곤충이 기록되었다. 가해능력이 우수하였던 곤충은 엉겅퀴의 우엉수염진딧물과 엉겅퀴가는잎말이나방이었고 쑥류에서는 쑥민들진딧물외에 쑥잎벌레, 긴발벼룩잎벌레, 금록색잎벌레, Hamushia konishili 잎벌레였다. 기주특이성이 있었던 곤충은 진딧물류와 잎벌레류였으며 쑥류와 망초의 꽃에서 빈번하게 채집되었던 노린재류의 기능은 확인할 수 없었다.

• 대한본초학회지
• /
• 제32권2호
• /
• pp.17-24
• /
• 2017

Objective : This study was designed to evaluate candidate materials as anti-inflammation agent from extracts of various Korean Compositae herbs in Hwaak mountain. Among Korea medicinal herbs, Ainsliaea acerifolia (AA) belongs to the Compositae family, has been used for the treatment of rheumatic arthritis. However, AA has not been previously reported to have an anti-inflammatory effect. Therefore, we investigated the anti-inflammatory effects of AA and its underlying molecular mechanisms in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Methods : Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in RAW 264.7 macrophages. Nitric oxide (NO) was measured with Griess reagent and pro-inflammatory cytokines were detected by enzyme immunoassay (EIA) kits in LPS-stimulated RAW 264.7 macrophages. Protein expressions of inducible nitric oxide synthase, and cyclooxygenase-2 (COX-2) and p65 subunit of nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) were determined by Western blot analysis. Results : Among 8 extracts of Korean Compositae herbs tested, AA showed the inhibition of NO production without cytotoxicity. Consistent with the observation, AA reduced the expression levels of iNOS and COX-2 proteins in LPS-simulated RAW 264.7 macrophages in dose-dependent manner. In addition, AA inhibited the productions of $TNF-{\alpha}$ and IL-6 in LPS-simulated RAW 264.7 macrophages. However, AA did not inhibit activation of p65 $NF-{\kappa}B$ in LPS-simulated RAW 264.7 macrophages. Conclusion : These results suggest that down-regulation of iNOS, COX-2 protein expression and $TNF-{\alpha}$ and IL-6 production by AA are responsible for its anti-inflammatory effects.

• 한국자원식물학회지
• /
• 제21권3호
• /
• pp.196-203
• /
• 2008

Phytotoxicity, antioxidant activity, and cytotoxicity of the aqueous or methanol extracts from the young sprouts of the six Compositae medicinal plants were determined. Aqueous leachates at 40g dry tissue $L^{-1}$ (g $L^{-1}$) Cirsium japonicum and Aster yomena showed the highest inhibitory effect on alfalfa (Medicago sativa L.). Total phenolic content showed the highest amount in methanol extracts from Ixeris dentata, and followed by A. yomena, and Cephalonoplos segetum. Methanol extracts of C. segetum and I. dentata at 25${\mu}g$ m$L^{-1}$ exhibited the highest DPPH radical scavenging activity by 87.2, and 52.8%, respectively. By means of HPLC analysis, MeOH extracts of C. segetum had the highest amount of antioxidant chlorogenic acid. Based on MTT assay, the methanol extracts from Y. sonchifolia ($IC_{50}$ = 65.7${\mu}g$ $mL^{-1}$) showed the highest cytotoxicity against Calu-6. These results suggest that plant extracts had a dose-dependent biological potentials including phytotoxicity, antioxidant activity, and anticancer activity, and that their activities exhibited differently depending on plant species.

• 한국식품영양과학회지
• /
• 제41권5호
• /
• pp.584-590
• /
• 2012

국화과 꽃은 우리나라에서 전통적으로 항염증과 항산화 치료에 사용되었다. 본 연구에서는 국화과 추출물이 인간 위암세포 AGS, 인간 유방암세포 MDA-MB-231과 SK-BR-3 암세포에서 성장을 억제하고 세포자멸사를 유발하는지 확인하였다. AGS, MDA-MB-231 그리고 SK-BR-3 암세포의 성장을 MTT로 측정하였다. 14종의 국화과 추출물을 24시간 동안 50, 100, 200 ${\mu}g/mL$의 농도로 처치하였다. 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부, 낙동구절초 전초, 산국 전초 그리고 해국 꽃 추출물에서 암세포의 성장을 농도 의존적으로 억제시켰다. 우리나라 여성에서 가장 많이 발생하는 유방암세포인 MDA-MB-231 암세포에서 세포자멸사를 확인하기 위해 DAPI 염색을 수행하였다. MTT assay에서 암세포를 억제시킨 6종의 국화과 추출물중 3종인 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부 추출물을 처치한 세포에서 핵의 응축이 농도 의존적으로 존재함을 형광현미경으로 확인하였다(${\times}200$). 세포자멸사에 관련된 단백질의 발현을 알아보기 위해서 western blot으로 확인하였다. 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부 추출물을 25, 50 ${\mu}g/mL$의 농도로 24시간 동안 MDA-MB-231 암세포 처치 후 cell lysate를 얻어 Bcl-2, Bax 그리고 p53의 변화를 관찰하였다. 한라구절초 전초, 포천구절초 전초 그리고 삼잎국화 지하부에서 anti-apoptotic 분자인 Bcl-2 단백질은 감소하고 반대로 pro-apoptotic 분자인 Bax와 p53 단백질은 증가하였다. 결과적으로 한라구절초 전초, 포천구절초 전초 그리고 삼잎국화 지하부 추출물은 유방암 세포의 성장을 억제하고 apoptosis를 유발시키므로 암예방제나 치료제로 개발될 수 있을 것으로 사료된다.

• Archives of Pharmacal Research
• /
• 제26권7호
• /
• pp.521-525
• /
• 2003

Phytochemical works on the aerial parts of Aster oharai (Compositae) led to the isolation of a new sesquiterpene hydroperoxide, 7$\alpha$-hydroperoxy-3, 11-eudesmadiene (2) and seven known compounds, teucdiol B (1), $\alpha$-spinasterol (3), oleanolic acid (4), $\alpha$-spinasterol 3-Ο-$\beta$-D-glucopyranoside (5), methyl 3,5-di-Ο-caffeoyl quinate (6), 3,5-di-Ο-caffeoylquinic acid (7), 3,4-di-Ο-caffeoylquinic acid (8). The chemical structures of 1-8 were established by chemical and spectroscopic methods. Compound 2 showed cytotoxicity against cultured human tumor cell lines in vitro, SK-OV-3 (ovarian), SK-MEL-2 (skin melanoma), and HCT15 (colon) with $ED_{50}$ values ranging from 3.86-17.21 $\mu$g/mL.

• 식물분류학회지
• /
• 제37권2호
• /
• pp.197-202
• /
• 2007

광주광역시 무등산에서 취나물속의 1종인 Saussurea nipponica subsp. higomontana 를 발견하여 국명을 무등취로 명명하였다.

• 한국환경복원기술학회지
• /
• 제8권4호
• /
• pp.52-67
• /
• 2005

The flora of the studied basin in the upper Keum River was listed as 237taxa; 63families, 162genera, 212species, 22varieties and 3forms. Based on the list of the ecosystem disturbance plants by the Ministry of Environment, 4taxa were recorded in the studied basin : Paspalum distichum(Gramineae), Paspalum distichum var. indutum(Gramineae), Ambrosia artemisiifolia var. elatior(Compositae), Ambrosia trifida(Compositae). Naturalized plants was listed as 54taxa; 16families, 41genera, 51species, 3varieties and naturalization index was 22.8percent of 1/4 the vascular plants. Upper Keum River was dominant floristic : indicator species were Salix gracilistyla community, Phragmites japonica community, native plants were Morus alba community, Spiraea prunifolia for. simpliciflora community, planting plants were Populus euramericana community, naturalized plants were Solidago serotina community, Helianthus tuberosus community.

• Journal of Life Science
• /
• 제12권1호
• /
• pp.9-13
• /
• 2002

Crepidiastrum lanceolatum ($H_{OUTT}$) $N_{AKAI}$ (Compositae) is distributed in East Asia including Korea. Genetic diversity and population structure of six C. lanceolatum populations in Korea and two populations in Japan were determined using genetic variation at 19 allozyme loci. The percent of polymorphic loci within the enzymes was 42.1%. Genetic diversity at the species level and at the population level was low (Hes : 0.077; Hep : 0.068, respectively), where-as the extent of the population divergence was relatively low ($G_{ST}$ : 0.093). One of the most striking features of this study was the more significant difference within populations than among populations. An indirect estimate of the number of migrants per generation (Nm : 2.44) indicated that gene flow was moderate among eight populations of the species. In addition, analysis of fixation indices revealed a slight heterozygosity deficiency in some populations and at some loci. Narrow geographic ranges, short-lived perennial herbaceous, and small population sizes are mainly associated with the low level of genetic variation.n.