• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.6019-6023
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• 2013

Lung cancer, one of the leading causes of cancer-related deaths, usually appears as solitary pulmonary nodules (SPNs) which are hard to diagnose using the naked eye. In this paper, curvelet-based textural features and clinical parameters are used with three prediction models [a multilevel model, a least absolute shrinkage and selection operator (LASSO) regression method, and a support vector machine (SVM)] to improve the diagnosis of benign and malignant SPNs. Dimensionality reduction of the original curvelet-based textural features was achieved using principal component analysis. In addition, non-conditional logistical regression was used to find clinical predictors among demographic parameters and morphological features. The results showed that, combined with 11 clinical predictors, the accuracy rates using 12 principal components were higher than those using the original curvelet-based textural features. To evaluate the models, 10-fold cross validation and back substitution were applied. The results obtained, respectively, were 0.8549 and 0.9221 for the LASSO method, 0.9443 and 0.9831 for SVM, and 0.8722 and 0.9722 for the multilevel model. All in all, it was found that using curvelet-based textural features after dimensionality reduction and using clinical predictors, the highest accuracy rate was achieved with SVM. The method may be used as an auxiliary tool to differentiate between benign and malignant SPNs in CT images.

• Journal of Life Science
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• v.29 no.1
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• pp.97-104
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• 2019

Streptococcus iniae is a typical fish pathogen causing streptococcosis and it can also cause zoonotic infectious diseases. We studied S. iniae FP5228 isolated from infected olive flounder in Wando, Korea. In a study to find virulence factors in FP5228, we found that the number of live bacteria decreased dramatically in culture medium containing S. iniae FP5228 for more than 24 hr. This phenomenon was hypothesized to be related to Toxin ${\zeta}$ and Antitoxin ${\varepsilon}$ genes, components of the Toxin/ Antitoxin (TA) system on the 14 kb plasmid of FP5228. We used a protein overexpression system to identify it. The pBP1140 vector system was constructed to regulate the expression of Toxin ${\zeta}$ and Antitoxin ${\varepsilon}$ by IPTG and Arabinose. E. coli/pBP1140 strain grew slowly in early growth under toxin expression condition, and it was confirmed by microscopic observation that the strain became longer. S. iniae CK287, lacking a 14 kb plasmid of S. iniae FP5228 strain, was constructed. CK287 bacterial cells did not show rapid killing during culture, and the ability to produce biofilm was also decreased, and toxicity was weakened in cytotoxicity test and fish test. These results suggest that the TA system is involved in physiological regulation and expression of virulence factors in S. iniae FP5228.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2000.11a
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• pp.21-22
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• 2000

Expressed sequence tags (EFTs) are the partial segments of cDNA produced from 5 or 3 single-pass sequencing of cDNA clones, error-prone and generated in highly redundant sets. Advancement and expansion of Genomics made biologists to generate huge amount of ESTs from variety of organisms-human, microorganisms as well as plants, and the cumulated number of ESTs is over 5.3 million, As the EST data being accumulate more rapidly, it becomes bigger that the needs of the EST analysis tools for extraction of biological meaning from EST data. Among the several needs of EST analyses, the extraction of protein sequence or functional motifs from ESTs are important for the identification of their function in vivo. To accomplish that purpose the precise and accurate identification of the region where the coding sequences (CDSs) is a crucial problem to solve primarily, and it will be helpful to extract and detect of genuine CD5s and protein motifs from EST collections. Although several public tools are available for EST analysis, there is not any one to accomplish the object. Furthermore, they are not targeted to the plant ESTs but human or microorganism. Thus, to correspond the urgent needs of collaborators deals with plant ESTs and to establish the analysis system to be used as general-purpose public software we constructed the pipelined-EST analysis system by integration of public software components. The software we used are as follows - Phred/Cross-match for the quality control and vector screening, NCBI Blast for the similarity searching, ICATools for the EST clustering, Phrap for EST contig assembly, and BLOCKS/Prosite for protein motif searching. The sample data set used for the construction and verification of this system was 1,386 ESTs from human intrathymic T-cells that verified using UniGene and Nr database of NCBI. The approach for the extraction of CDSs from sample data set was carried out by comparison between sample data and protein sequences/motif database, determining matched protein sequences/motifs that agree with our defined parameters, and extracting the regions that shows similarities. In recent future, in addition to these components, it is supposed to be also integrated into our system and served that the software for the peptide mass spectrometry fingerprint analysis, one of the proteomics fields. This pipelined-EST analysis system will extend our knowledge on the plant ESTs and proteins by identification of unknown-genes.

• The Korean Journal of Pesticide Science
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• v.12 no.4
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• pp.391-396
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• 2008

The pine sawyer, Monochamus saltuarius Gebler (Colepotera: Cerambycidae) was newly known as a vector of pinewood nematode causing the pine wilt disease in Korean white pine (P. koraiensis Siebold & Zucc.) forestry. Recently, it has a serious problem to spread fast and widely throughout Korea by transferring M. saltuarius. For the control of M. saltuarius, this study was investigated the attractants by analyzing from the Korean white pine. Major components of Korean white pine was analyzed as $\alpha$-pinene and ethanol, and reported as an attractant against Monochamus genus. Ipsenol and ipsdienol, the pheromones of Ips genus known as attractant components of woodboring beetles, were tested to the attractive efficacy against Monochamus saltuarius. The attractive efficacy showed 67.9% of Ipsenol plus ipsdienol and ipsenol, ipsdienol mixed with $\alpha$-pinene plus ethanol was showed 67.7% and 79.3%, respectively. Especially, ipsdienol with $\alpha$-pinene and ethanol was shown the high synergy effect, and the attractant effect was higher at M. saltuarius female than male.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.8
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• pp.3933-3940
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• 2013

The purpose of present study was to determine the frequency of RA with age and to investigate the age-related trends and changing-factors in RA, CA and IAs. The refractive power of the eye and the power of corneal anterior surface were measured with auto-refractor among 1,017 inhabitants aged 5 to 59 years in Cheongju in July 2010. The overall frequency of RA was 22.7%, and frequency of RA increased from 8.9% in 5~9 years age group to 36.8% in 20~29 years age group. It then dipped to 19.2% in 40~49 years age group but increased again 28.6% in 50~59 years age group. $J_{45}$ components for RA, CA, and IAs were fairly stable in different age groups, the changes in $J_0$ components for both RA and CA appeared to be decreased after age of 30 years. In addition, the refractive power on the vertical direction was changed slightly with age, but the refractive power on the horizontal direction was changed significantly with age. It was expected that the change in the frequency of astigmatism with age was due to the change in the refractive power of horizontal meridian.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.19 no.5
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• pp.547-555
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• 2008

This parer proposed the path mismatch detection and compensation algorithm with closed form for linear amplification with non-linear components(LINC) system implementation. The LINC system has a merit of using the high efficient amplifier by transferring the non-constant envelop signal which is high peak to average signal ratio into constant envelop signal. However, the performance degradation is very sensitive to the path mismatch such as an amplitude mismatch and a phase mismatch. In order to improve the path mismatch, the error detection and compensation method is introduced by the use of four test signals. Since the presented method has the closed form solution, the efficient and fast detection is available. The digital-IF structure of LINC system applied by the proposed error detection and compensation algorithm was implemented. The performance was evaluated with the IEEE 802.16 WiMAX baseband sinal which has 7 MHz channel bandwidth and 16-QAM. The Error Vector Magnitude(EVM) of -37.37 dB was obtained through performance test, which meets performance requirement of -24 dB EVM. As a result, the introduced error detection and compensation method was verified to improve the LINC system performance.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.9
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• pp.5621-5627
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• 2014

To specify the standard of LASEK surgery, this study examined the determination factors affecting the high order aberrations (HOAs) in preoperative and postoperative LASEK. 51 adult patients (102 eyes) were evaluated at the baseline and 2 months after LASEK surgery from Nov 2011 to Jul 2012. The postoperative HOAs ($0.538{\mu}m$) were higher than the preoperative ($0.341{\mu}m$). In linear regression analysis, the refractive components that affected the postoperative HOAs were $J_0$ of corneal astigmatism (CA, 0.400), $J_0$ of refractive astigmatism (RA, 0.389), corneal astigmatism (CA, 0.282), spherical equivalent (SE, 0.239), refractive astigmatism (RA, 0.213), and pupil size (PS, 0.194) with a high R. In multiple regression, $J_0$ of CA, PS and SE were significant factors with the postoperative HOAs. In conclusion, both HOAs and $J_0$ of CA should be considered when determining the suitable factors for LASEK surgery.

• The Journal of the Acoustical Society of Korea
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• v.23 no.1
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• pp.8-16
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• 2004

An inversion method is presented for the determination of the compressional wave speed, compressional wave attenuation, thickness of the sediment layer and density as a function of depth for a horizontally stratified ocean bottom. An experiment for estimating those properties was conducted in the shallow water of South Sea in Korea. In the experiment, a light bulb implosion and the propagating sound were measured using a VLA (vertical line array). As a method for estimating the geoacoustic properties, a coherent broadband matched field processing combined with Genetic Algorithm was employed. When a time-dependent signal is very short, the Fourier transform results are not accurate, since the frequency components are not locatable in time and the windowed Fourier transform is limited by the length of the window. However, it is possible to do this using the wavelet transform a transform that yields a time-frequency representation of a signal. In this study, this transform is used to identify and extract the acoustic components from multipath time series. The inversion is formulated as an optimization problem which maximizes the cost function defined as a normalized correlation between the measured and modeled signals in the wavelet transform coefficient vector. The experiments and procedures for deploying the light bulbs and the coherent broadband inversion method are described, and the estimated geoacoustic profile in the vicinity of the VLA site is presented.

• Journal of KIISE:Databases
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• v.33 no.4
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• pp.372-383
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• 2006

Among the approaches to image database management, content-based image retrieval (CBIR) is viewed as having the best support for effective searching and browsing of large digital image libraries. Typical CBIR systems allow a user to provide a query image, from which low-level features are extracted and used to find 'similar' images in a database. However, there exists the semantic gap between human visual perception and low-level representations. An effective methodology for overcoming this semantic gap involves relevance feedback to perform feature re-weighting. Current approaches to feature re-weighting require the number of components for a feature representation to be the same for every image in consideration. Following this assumption, they map each component to an axis in the n-dimensional space, which we call the metric space; likewise the feature representation is stored in a fixed-length vector. However, with the emergence of features that do not have a fixed number of components in their representation, existing feature re-weighting approaches are invalidated. In this paper we propose a feature re-weighting technique that supports features regardless of whether or not they can be mapped into a metric space. Our approach analyses the feature distances calculated between the query image and the images in the database. Two-sided confidence intervals are used with the distances to obtain the information for feature re-weighting. There is no restriction on how the distances are calculated for each feature. This provides freedom for how feature representations are structured, i.e. there is no requirement for features to be represented in fixed-length vectors or metric space. Our experimental results show the effectiveness of our approach and in a comparison with other work, we can see how it outperforms previous work.

• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.1061-1065
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• 2001

The antibacterial effects of water extracts of Scutellariate Radix (a dried root of Scutellaria baicalensis GEORGI) and its major flavonoid components, Baicalin and Baicalein, on Salmonella typhimurium, a representative enteric pathogen, were studied. Through a Kriby-Bauer disc analysis, the growth-inhibition activity of Scutellariae Radix against. S. typhimurium was found to be compatible with commercial antibiotics, such as ampicillin, chloramphenicol, and streptomycin. In contrast, the growth of a nonpathogenic E. coli strain was unaffercted by Scutellariae Radix. To examine the effect of polyphosphate kinase (ppk), a putative virulence factor, on the antibacterial activity of Scutellariae Radix, the growth profile of a ppk mutant of S. typhimurium was investigated in a tryptic soy broth containing different concentrations of water extracts of Scutellariae Radix. The ppk mutant was able to grow in 6 mg/ml of water extracts of Scutellariae Radix, whereas in 6 mg/ml of water extracts of Scutellariae Radix, whereas the wild-type could not, implying that the inactivation of ppk made S. typhimurium more resistant to the antibacterial activity of Scutellariae Radix. No enhanced resistance was observed in a ppk mutant of S. typhimurium complemented with a ppk expression vector. The attenuation of the virulence by ppk inactivation was also observed in a virulence assay using BLAB/c mice. Neither Baicalin nor Baicalein exhibited any growth-inhibition activity against S. typhimurium. The water extracts of Scutellariae Radix stimulated the transcription of ppk, especially in the early growth-stage of S. typhimurium.