• 제목/요약/키워드: component sugar

검색결과 323건 처리시간 0.019초

추출조건에 따른 차가버섯 생리활성 및 면역활성 다당 (Pharmacological Activity of Chaga Mushroom on Extraction Conditions and Immunostimulating Polysaccharide)

  • 백길훈;정헌상;김훈;윤택준;서형주;유광원
    • 한국식품영양과학회지
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    • 제41권10호
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    • pp.1378-1387
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    • 2012
  • 추출조건에 따른 생리활성 영향을 검토하기 위하여 차가버섯(Inonotus obliquus)을 물($50^{\circ}C$ reflux, $90^{\circ}C$ 이상 decoction, $12^{\circ}C$ 가압추출)과 에탄올(50, 70과 $90^{\circ}C$ reflux)로 추출한 후 물 추출물을 조다당획분(IO-CP)으로 분획하였을 때 IO-CP(4.8~16.8%)의 수율은 에탄올추출물(IO-E, 1.9~2.7%)보다 높았으며 추출온도 증가에 따라 그 차이는 더 컸다. 가압추출로 조제된 조다당획분(IO-CP-121)은 가장 높은 총 폴리페놀 및 플라보노이드 함량(35.10 mg TAE/g와 18.48 mg QE/g)과 DPPH와 ABTS를 이용한 자유 라디칼소거능(26.08과 27.99 mg AEAC/100 mg)을 나타내었다. IO-CP-D(decoction)와 IO-CP-50(reflux)은 IO-CP-121보다 유의적으로 높은 마이토젠(saline 대조군의 2.10과 1.95배, 100 ${\mu}g/mL$)과 장관면역 활성(6.30과 5.74배)을 보였으나 에탄올추출물 활성은 확인되지 않았다. 또한, 모든 IO-CP는 0.1 mg/mL 농도에서 RAW 264.7 세포주에 대하여 독성을 나타내지 않았으나, IO-CP-121이 LPS-자극 RAW 264.7 세포주에 대하여 가장 유의적으로 염증성 인자인 TNF-${\alpha}$와 nitric oxide(NO) 생성을 억제하였다(29.2와 63.5%). 에탄올추출물도 0.1 mg/mL 농도에서 독성을 보이지 않았으나 TNF-${\alpha}$와 NO의 생성 억제능은 IO-CP-121보다 현저히 낮음을 알수 있었다. 한편, 활성 다당획분을 분리하기 위하여 면역활성 획분인 IO-CP-D를 DEAE-Sepharose CL-6B column에서 비흡착 획분(IO-CP-I)과 7개의 흡착 획분(IO-CP-II~VIII)으로 분획한 결과, IO-CP-II가 가장 우수한 마이토젠과 마크로파지 활성(4.51과 1.64배)을 나타내었다. 이와 같이 분리된 면역활성 획분인 IO-CP-II는 주로 중성당(61.86%)과 함께 소량의 산성당(2.96%)을 포함하고 있는 다당류임이 밝혀졌으며, 주요 구성당으로서 Glc, Gal와 Man(molar ratio of 1.00:0.55:0.31)를 포함하고 있음을 확인할 수 있었다. 이러한 결과로부터 추출조건은 차가버섯 생리활성에 중요하게 작용하고 있으며, decoction 추출방법으로 제조하여 분획한 면역활성 획분으로부터 차가버섯 면역활성은 적어도 중성 다당류가 관여하고 있음을 알 수 있었다.

장수버섯 균사체가 배양된 옻피의 이화학적 특성 및 생리활성 (Physicochemical Properties and Physiological Activities of Rhus verniciflua Stem Bark Cultured with Fomitella fraxinea)

  • 최한석;김보현;여수환;정석태;최지호;박효숙;김명곤
    • 한국균학회지
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    • 제38권2호
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    • pp.172-178
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    • 2010
  • 옻피의 일반성분은 수분 1.64%, 지방 8.09%, 단백질 7.28%, 회분 6.48%, 당류 5.39%로 구성되어 있었던 반면, 장수버섯을 증식시킨 옻나무 껍질은 각각 7.64%, 3.86%, 3.59%, 6.30%, 불검출 이었다. 배양물의 총 유리아미노산 함량은 97.41mg%으로 대조구(71.91 mg%)에 비하여 35% 증가하였으며 phosphoserine(55.06 mg%), ammonia(17.84 mg%), aspartic acid (6.05 mg%)가 주요 유리아미노산 이었다. 배양물의 총 phenolic acid함량은 422.89 ppm/283.86 ppm(알코올/물 추출물)이었으며 syringic acid와 gallic acid가 주요 성분이었고 vanillic, protocatechuic acid가 소량 함유되어 있었다. 항산화활성(DPPH, $IC_{50}$)은 $28.54\;{\mu}g/54.70\;{\mu}g$ (알코올/물 추출물)으로 gallic acid가($IC_{50}\;1.84\;{\mu}g$) 주요 영향 요소이었다. 배양물의 NIH3T3세포에 세포독성($IC_{50}$)은 알코올 추출물 $50\;{\mu}g/200\;{\mu}L$, 물 추출물 $90\;{\mu}g/200\;{\mu}L$이었으며 UV조사에 의해 유도된 산화적 stress에 대하여 $20-25\;{\mu}g/200\;{\mu}L$ 농도에서 항산화 활성을 보였다. 배양물의 물 추출은 전구지방세포(3T3-L1)의 분화를 촉진시켰으며 $10\;{\mu}g/200\;{\mu}L$의 농도에서 가장높았다.

국민식생활(國民食生活) 향상(向上)을 위(爲)한 곡류제품(穀類製品)의 경제적( 經濟的) 영향강화(營養强化)에 관(關)한 연구(硏究) (A Study on the Economical Nutrition Supplement of Cereal Food for Improvement in our National Eating Habits)

  • 주진순;유종열;김숙희;이기열;한인규
    • Journal of Nutrition and Health
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    • 제6권1호
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    • pp.1-9
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    • 1973
  • I. Subject of the Study: Studies on the economical nutrition supplement of cereal foods for the improvement in our notional eating habits. II. Purpose and Importance of the Study: 1. Our nation is confronted with the situation that the rice, a principal food, short of some essential amino acids, lysine and threonine, leads to imbalanced meals insufficient in the nutrient of protein, to bring many difficulties in the elevation of our national physique. 2. The shortage of even the rice imperfect in the nutrient of protein makes the import of lots of foreign rice inevitable. It is considered that the protein supplement and decrease in the consumption amount, of rice, is a serious key to the solution of our food difficulty, and then a way of the proetin supplement of rice through the addition of essential amino acid is to be rarely applied in the view of the our present finance and situation. 3. In the present experiment, therefore, it aims to the suggestion of an aspect of the improvement in our national eating habits guiding in the nutrition elevation which our nation can afford economically through the development of first, a way of the protein supplement by the mixture of cereals producted plentifully in our country, and second, a way of the decrease in the consumption amount and the improvement in the nutrition of rice through the substitution of the other cereals for rice. III. Contents of Scope of the Study: 1. Objects of the study: Objects of the study are the following three items; a) The nutrition supplement of rice through the mixture of cereals. Our nation makes mainly rice as a principal food, but practically many kinds of cereal are produced in our country. They contain different levels and qualities of each nutrient and they are different from one another in the kinds of essential amino acid consisting protein. For that reason, the mutual complement efficeincy of insufficient nutrients is observed through the mixture of cereals. b) The nutrition supplement of rice through the addition of superior protein sources to rice, a principal food. The development of rice as superior foods in the sense of nutrition is conducted through the risement in protein quality by the addition of protein sources in good quality, for example, fish flour (anchovy flour), egg powder, milk powder, and so on, and through the supplement of vitamins and minerals. c) The decrease in the consumption amount of rice through the substitution of the other cereals for rice, as a principal food. The compensation for the short amount of rice is made by the reduction in the consumption of rice through the discovery of a way of substitution of the other cereals for rice, as a principal food and of the settlement of problems in nutrition and finance subsequent to this. 2. Contents of the study: a) An ideal mixture-ratio of cereals is established for rats by feeding mixed foods(rice-barely or rice-wheat) containing 5%, 15%, 25%, 35% and 45% level of either barely or wheat. b) The nutritive value is determined in the whole subsititution of other foods for rice, and then, a way of the complement of over and under nutrients is devised. c) The ideal combination is investigated for rats through feeding mixed foods of main food, rice and supplement foods of protein sources, soy bean, fish flour, egg powder and milk Powder. d) According to results from the above three experiments, the concise functional test for men and the examination of economical property are made. 3. Scope of the study: a) The observation of the effect of each diet on the growth rate for rats. The growth rate of rats was observed for 15 groups of mixed foods of a main food, rice, and wheat flour, barley powder or soy bean powder, respectively, and 12 groups of wheat flour diets supplemented with $1{\sim}3%$ milk powder, and rice or wheat flour diets supplemented with 5% of milk powder, egg powder, fish flour or soy bean powder, respectively. b) The determination of food consumption. The food consumption was determined at weekly intervals for 27 kinds of diet described in a) item. c) The determination of food efficiency rate. The food efficiency rate for each diet was determined by calculation from the gained body weight and the food consumption amount at the same intervals described in b) item. d) The determination of protein efficiency rate. The protein efficiency rate for each diet was determined by calculation form gained body weight and the protein amount of the food consumption amount at the same intervals described c) item. e) The determination of the body component. The hematocrite and hemoglobin levels in the blood, total nitrogen in the serum, blood sugar, and lipids and glycogen in the liver were determined. f) The observation of nitrogen balance. As a means of the observation of nitrogen balance, the total nitrogen in the urine was determined. g) The analysis of economical property. The economical property was analyzed as the gained body weight to the amount equivalent to one won through the conversion of the food consumption amount into money. h) The functional test for men. The concise functional test for men was made in order to establish if the best diet for experimental animals can be applied to men. IV. Results of the Study: The national food product plan, nationwide nutritive enlightment and the improvement activities in our country eating habits, especially, mixed and powder food problems are to be significantly referred, and the following results must be applied. a) In the mixed foods of cereals, the mixed food of the rice-barley containing $5{\sim}15%$ level of barley is best in terms of nutrition. b) The addition of superior protein sources, egg, Bilk, soy bean, or fish, respectively to either rice or wheat flour makes a great risement in the nutritive value. c) The animal protein is more effective in the elevation of nutritive value of cereals. d) Rice takes the most nutritive operation and has the highest preference, among rice, wheat flour and barley. e) Wheat flour is more economical than rice in evaluation of the gained body weight to the regular money, and the addition of fish or soy bean is more economical than that of any other supplement food. But the above results are true of the range of nutrition and economical property. f) The study on the nutrition composition and barley will lead to the improvement in our national eating habits as mixed food of the rice-barley containing $5{\sim}15%$ level of barley is more nutritive. g) This study on the nutrition only for the growing animal can not be considered as a perfect and entire evaluation. Consequently, the perfect data for our national nutrition can be obtained from the experiment similar to this for the much longer period examining, in details, the growth rate, change of physical strength, mental and bodily change, average life span, and resistance ability to infectious diseases.

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