• Journal of Periodontal and Implant Science
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• 제25권2호
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• pp.181-191
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• 1995

The ability of fibroblasts attach to teeth is of paramount imporance in re-establishing the lost connective tissue attachment after periodontal therapy. Tobacco contains a complex mixture of substances including nicotine. various nitrousamines, trace elements. and a variety of poorly characterized substances. The effects of nicotine on fibroblasts have reported an altered morphology and attachment of fibroblasts to substrates and disturbances in protein synthesis and secretion. This study examined the effect of nicotine, a major component of the particulate phase of tobacco smoke, on human gingival fibroblasts and periodontal ligament cells attachment to tissue culture surfaces and cellular activity of human gingival fibroblasts and periodontal ligament cells. Pooled human gingival fibroblasts made from extraction of 3rd molar were utilized between passage 4 and 5 and plated in 96 well plate at 20,000 cells per well. Cell number were determined using 3-(4,5-dimethylthiazole-2-y)2,5-diphenyltetrazolium bromide(MTI) , which is reflection of mitochondrial dehydrogenase activity. The concentration of nicotine used were 0.025, 0.05, 0.1, 0.2 and $0.4{\mu}M$, the average serum concentration for a smoker being approximately $0.1{\mu}M$. The results were as follows : 1. Attachment effects of nicotine on human gingival fibroblasts and periodontal ligament cells Excepts of $0.4{\mu}M$, the effects on attachment with increasing numbers of cells attaching with increasing nicotine concentrations, compared to control group. But over the 60min, return to control value. 2. The effect of cellular activity on human gingival fibroblasts and periodontal ligament cells. The cellular activity of human gingival fibroblasts and periodontal ligament cells were similar or decrease to control value at 1st incubation day. At 2nd incubation day, 0.05, 0.1, 0.2, $0.4{\mu}M$ concentrations were statistically different from control value on gingival fibroblasts group. But at 3rd incubation day, cellular activities of all experimental group were significantly decrease than control group.

• Bulletin of the Korean Chemical Society
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• 제32권2호
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• pp.639-644
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• 2011

A simple and highly sensitive chemiluminescence method to determine norfloxacin (NFLX) has been proposed by measuring the chemiluminescence (CL) intensities using a flow injection (FI) system. The CL intensity of the luminol-$H_2O_2$ system is strongly enhanced by the addition of Cu (II) in alkaline condition. The CL intensity is substantially increased after the injection of NFLX into the luminol-$H_2O_2$-Cu (II) system. The enhancement effect is attributed to a catalytic effect of Cu (II) due to the interaction with NFLX which forms a complex with the catalyst. Under the optimal conditions, the sensitizing effect of the CL intensity is proportional to the concentration of NFLX in the range of $1.5{\times}10^{-9}-5.9{\times}10^{-7}molL^{-1}$ (r = 0.9994) with a detection limit ($3{\sigma}$) of $2.98{\times}10^{-10}molL^{-1}$. The proposed method had good reproducibility with the relative standard deviation (RSD, n = 5) of 1.6% for $1{\times}10^{-7}molL^{-1}$ of NFLX. The possible reaction mechanism of the CL reaction is also discussed. This method has been successfully applied for the determination of trace amount of NFLX in pharmaceutical preparations and serum samples.

• 한국환경보건학회지
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• 제32권5호
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• pp.522-531
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• 2006

This study has been conducted to evaluate the influence of ambient heavy metals to the air quality and to find their sources in Ulsan. Korea. The distribution characteristics of ambient heavy metals with wind direction were investigated by using pollution-rose diagram. Carcinogenic risk for five trace heavy metals (Pb. Cd, Cr, Mn, and Ni), which are classified to carcinogenic material by US EPA, are assessed according to the classification system of USEPA and WHO. According to pollution-rose calculation on pollutants sources, Pb and Cu in Deoksin, which is an adjacent to Onsan industrial complexes, were $0.1058\;and\;0.3242{\mu}g/m^3$. These concentration levels are the highest at all sampling sites. From this result we could confirm that it was affected by Onsan industrial complex that is located northeast of Deoksin.,And the maximum concentration of Cd, Cr, and Ni were $0.0306,\;0.0102,\;and\;0.0146\;{\mu}g/m^{3} in Yeocheon respectively. Because Yeocheon is in the Mipo industrial complexes, which have many combustors, incinerators, and manufacturing facilities compared to other regions. In carcinogenic risk assessment, Pb, Ni, and Mn concentration level at six sampling sites were lower than the risk level guideline values ($10^{-5}$) of WHO. However, $Cr^{6+}$ and Cd concentration in Yeocheon were 60% and 205% higher than the guideline values of WHO. Therefore it is very important that the emission from industrial complexes were carefully managed and controlled to improve air quality in residential area.

• Journal of Periodontal and Implant Science
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• 제26권1호
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• pp.176-187
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• 1996

The ability of fibroblasts attached to teeth is paramount important in reestablishing the lost connective tissue attachment after periodontal therapy. The migration and proliferation of periodontal ligament cells are desired goal of periodontal regeneration therapy. PDGF is well known to regulate the cell activity of mesenchymal origin cell. Tobacco contains a complex mixture of substance including nicotine, various nitrosamines, trace elements, and variety of poorly characterized substances. Human gingival fibroblasts and periodontal ligament cells were cultured from extracted tooth for non-periodontal reason. Cultured human gingival fibroblasts and periodontal ligament cells in vitro were treated with PDGF, nicotine in time dependent manner. Cellular activities were determined by MTT assay. The purpose of this study was to determine the effects of Nicotine and PDGF, respectively and the effect of PDGF presence of nicotine on human gingival fibroblasts and periodontal ligament cells. The results were as follows : 1. In the cell activities of human gingival fibroblasts and periodontal ligament cells were similar or decreased to control value at 1st day. At 2nd day, cellular activities of both group were increased to control value. At 3rd day, cellular activities of both group were returned to the control value. 2. In the cell activities of PDGF on human gingival fibroblasts and periodontal ligament cells, cell activities significantly increase from control group on periodontal ligament cells compared to gingival fibroblast group at 3rd day. 3. In the cell activities of PDGF and nicotine combined application on human gingival fibroblasts and periodontal ligament cells, it seems likely that the nicotinic effect of gingival fibroblasts were higher than periodontal ligament cells and the PDGF effect of periodontal ligament cells were higher than gingival fibroblasts. This results suggested that PDGF might stimulate the selective growth on periodontal ligament cells.

• 한국정보과학회논문지:시스템및이론
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• 제26권9호
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• pp.1126-1134
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• 1999

최근 다양한 멀티미디어 서비스를 지원하기 위한 통신망 관리와 서비스 관리가 통합된 개방형 정보 통신망의 구조가 요구되고 있다. 이러한 구조를 기반으로, TINA-C(Tele-communications Information Networking Architecture-Consortium)에서는 분산 환경에서 분산 어플리케이션 객체들에 대한 복잡한 서비스 및 관리 인터페이스들을 간결하도록 객체그룹의 개념을 정의하고 있다. 이러한 환경 속에서 트레이딩 서비스는 통신 서비스를 제공하는 객체그룹간의 상호작용을 지원하는데 매우 중요하다.따라서, 본 논문에서는 객체그룹간의 상호접속을 지원할 수 있는 트레이딩 기능과 이들 트레이더들간의 연동을 위한 트레이더 연합 모델을 제시하고자 한다. 이를 위해 우리는 트레이더의 중개자로서 Cooperator를 설계하여 기존의 트레이더와 연동시켰다. 이러한 결과로서 우리의 새로운 트레이더 연합 모델에서 Cooperator는 객체그룹간의 상호접속에서 객체들의 접속 권한의 체크 기능과 기존의 트레이더 연합모델의 문제점인 트레이더들간의 단 방향 연결문제를 보완하여 양방향 연결 기능을 갖도록 하였다. 끝으로, 이러한 해결 과정을 보이기 위해 트레이더와 Cooperator들로 이루어진 본 연합모델에서 분산 객체그룹간의 상호접속 절차과정과 사건 추적 다이어그램을 보였다.Abstract Recently, the open networking architecture is required to support various multimedia services as integrated functions of network management and service management. Based on this architecture, TINA-C defines an object group concept for simplifying complex management and service interfaces, when distributed application is executed in distributed environments. Within the support environment the trading service is an important of the interacting object groups which provide a telecommunication service.Hence, we suggest the trader federation model for supporting interconnections between object groups and among existing traders by using the cooperator we designed, as an intermediator among traders. Our cooperator has functions for checking access rights of objects in object groups, and for providing bidirectional linkage among traders. Up to now, the existing trader federation models have a single directional linkage for interactions among traders. Finally, we showed the interface procedure and the event trace diagram of distributed object groups using our model consisted of traders and the cooperators.

• 기술사
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• 제19권4호
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• pp.22-29
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• 1986

1) The recipe for tea is to mix tea leaves or tea buds with odorous pharmaceuctial materials, which are boiled and drunk, addition of honey only to that odorous pharmaceutical meterials without adding tea buds to that can be used. 2) Viewed from the part of tea plant the fruit of it accoupies most. The cereal does as much. 3) what we have referred to from the records on this subject are "Guogapylyong" which is of the yuan dynasty and "Jeungbo-Salymkeongje" which is of the Yi dynasty, based on which no trace of pure tea can be found but odorous pharmaceutical kinds of tea only were widely spread. 4) The characteristics of tea are to help make stomach strong, mind cleared, the lungs copious, counteract, and quench thirstiness. The tea help build liver as well as bring about health. 5) As a result of sense test, those kinds of tea were much better than contemporary kinds of teas as far as flovor and sweetness are concerned. Those teas had complex tastes, while teas of to day have simple one. 6) Because the history not so clear before the periods of Unified Sila that the recordings of teas are rare, we can hardly know about tea. Our ancestors really began to drink teas from the period of Unified Sila on. This country turning to the Koryo dynasty, tea drinking manner began to prevail so much that drinking tea became a ceremony. Daring the Yi dynasty it was much camplicated to make and boil the leaf tea. As a result of the previous fact pure tea came to have disappeared and odorous pharmaceutical became prevailent. 7) For value of exploiting Kungjuk tea (ginger tea seasoned with phyllostachy and addition of honeyl) was the highest among many. The worth of developing all the teas among all the beverages was regarded as that high of 51%, while even the tea regarded as worthless obtained 4％ of support, which is the lowest.

• 한국지하수토양환경학회지:지하수토양환경
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• 제20권1호
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• pp.19-27
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• 2015

If $CO_2$ stored for geological sequestration escapes from deep formations and is introduced to shallow aquifers, it dissolves into groundwater, creates acidic environments, and enhance mineral dissolution from rocks and soils. Among these minerals, dissolution and spread of hazardous trace metals can cause environmental problems with detrimental impacts on groundwater quality. This study aims to investigate geochemical effects of $CO_2$ in groundwater on dissolution of galena, the main mineral controlling the mobility of lead. A series of batch experiments are performed with granulated galena in $CO_2$ solutions under various experimental conditions for $CO_2$ concentration and reaction temperature. Results show that dissolution of galena is significantly enhanced under acidic environments so that both of equilibrium concentrations and dissolution rates of lead increase. For thermodynamic analysis on galena dissolution, the apparent rate constants and the activation energy for galena dissolution are calculated by applying rate law to experimental results. The apparent rate constants are $6.71{\times}10^{-8}mol/l{\cdot}sec$ at $15^{\circ}C$, $1.77{\times}10^{-7}mol/l{\cdot}sec$ at $25^{\circ}C$, $3.97{\times}10^{-7}mol/l{\cdot}sec$ at $35^{\circ}C$ and the activation energy is 63.68 kJ/mol. The galena dissolution is suggested to be a chemically controlled surface reaction, and the rate determining step is the dissociation of Pb-S bond of surface complex.

• 융합정보논문지
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• 제10권1호
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• pp.13-21
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• 2020

인터넷 기술 및 디지털 콘텐츠 편집 소프트웨어의 발전으로 인해 점점 많은 수의 1인 미디어 창작자(크리에이터)들이 다양한 콘텐츠들을 생산하고 공유하고 있다. 그러나 1인 미디어 활성화를 위해서는 콘텐츠의 사용, 판매, 편집, 재가공 등 다양한 거래 유형별 이용권한(라이선스) 생성과 함께 콘텐츠 사용 모니터링, 라이선스 거래 추적 등의 핵심 기술이 1인 미디어 플랫폼에서 지원되어야 한다. 본 논문에서는 ODRL(Open Digital Rights Language) 온톨로지(ontology)에 기반하여 다양한 수준의 디지털 콘텐츠 이용권한 및 이용추적을 명세할 수 있는 Policy 클래스 확장 모델을 정의하고 모델의 성능을 평가하기 위한 사용자 인터페이스 프로토타입을 구현하였다. 본 논문의 제안 모델은 개인 창작물 공유 플랫폼의 라이선스 관리 모듈 구현의 핵심 요소로 참조될 수 있다.

• Bulletin of the Korean Chemical Society
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• 제33권12호
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• pp.4145-4149
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• 2012

A novel and simple method of luminescence enhancement effect for the determination of trace amounts of bile acid was proposed. The procedure was based on the luminescence intensity of the balofloxacin-europium(III) complex that could be strongly enhanced by bile acid in the presence of sodium dodecyl benzene sulfonate (SDBS). Under the optimum conditions, the enhanced luminescence intensity of the system exhibited a good linear relationship with the bile acid concentration in the range $5.0{\times}10^{-9}-7.0{\times}10^{-7}\;mol\;L^{-1}$ with a detection limit of $1.3{\times}10^{-9}\;mol\;L^{-1}$ ($3{\sigma}$). The relative standard deviation (RSD) was 1.7% (n = 11) for $5.0{\times}10^{-8}\;mol\;L^{-1}$ bile acid. The applicability of the method to the determination of bile acid was demonstrated by investigating the effect of potential interferences and by analyzing human serum and urine samples. The possible enhancement mechanism of luminescence intensity in balofloxacin-europium(III)-bile acid-SDBS system was also discussed briefly.

• 농업과학연구
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• 제22권1호
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• pp.11-23
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• 1995

The CAD/CAM system for the manufacturing automation is the newest technology in mechanical engineering area and becomes the important research subject nowadays. Most of all hardwares and softwares for the CAD/CAM system used in the our manufacturing companies such as automobile company are developed by the foreign country and the purchasing price of them is very expensive but their applicability to a certain area is very limited. This study was conducted to develope a CAD/CAM system for the design and the automatic manufacturing of the iron pattern shaped with 3 - dimensional free curved surface, and to test its applicability to the design and the manufacturing of the rotary blade. The results obtained from the study are as follow; 1. The CAD system which can process graphic procedures from the free curved surface shaped data was developed with personal computer. 2. The CAM main program was developed. This main program could produce CL data from CAD data file by checking the tool interference according to the cutting mode. 3. The sub. program which can simulate the tool trace from the CL data was developed. 4. The post processor for the Deckel FP2NC NC milling machine from CL data file was developed and the sub program could transmit NC program through modem to NC milling machine was developed. 5. The developed CAM system seemed to be applicable to any other system. Because the measuring results of the cross sectional thickness of the plastic model from the manufacturing iron pattern by the system showed that this system could properly check the tool interference. 6. In took 75~90 hours to manufacture two iron patterns of rotary blade. For the sake of convenience in applying to the other systems, this system was developed in BASIC and FORTRAN computer language and minimum portion of machine language as possible.