• Bulletin of the Korean Chemical Society
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• 제27권2호
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• pp.224-230
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• 2006

Escherichia coli transcription termination factor Rho catalyzes the unwinding of RNA/DNA duplex in reactions that are coupled to ATP binding and hydrolysis. We report here the kinetic mechanism of presteady state ATP binding and hydrolysis by the Rho-RNA complex. Presteady state chemical quenched-flow technique under multiple turnover condition was used to probe the kinetics of ATP binding and hydrolysis by the Rho-RNA complex. The quenched-flow presteady state kinetics of ATP hydrolysis studies show that three ATPs are bound to the Rho-RNA complex with a rate of $4.4\;{\times}\;10^5M^{-1}s^{-1}$, which are subsequently hydrolyzed at a rate of $88s^{-1}$ and released during the initiation process. Global fit of the presteady state ATP hydrolysis kinetic data suggests that a rapid-equilibrium binding of ATP to Rho-RNA complex occurs prior to the first turnover and the chemistry step is not reversible. The initial burst of three ATPs hydrolysis was proposed to be involved in the initialization step that accompanies proper complex formation of Rho-RNA. Based on these results a kinetic model for initiation process for Rho-RNA complex was proposed relating the mechanism of ATP binding and hydrolysis by Rho to the structural transitions of Rho-RNA complex to reach the steady state phase, which is implicated during translocation along the RNA.

• 센서학회지
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• 제27권6호
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• pp.392-396
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• 2018

Structural color has many advantages over pigment based color. In recent years, researches are being conducted to apply these advantages to applications such as wearable devices. In this study, strain sensor, a kind of wearable device, was developed using structural color. The use of structural color has the advantage of not using energy and complex measuring equipment to measure strain rate. Wrinkle structure was fabricated on the surface of Poly-dimethylsiloxane (PDMS) and used it as a sensor which color changes according to the applied strain. In addition, a transmittance-changing sensor was developed and fabricated by synthesizing additional glass nanoparticles. Furthermore, a strain sensor was developed that is largely transparent at the target strain and opaque otherwise.

• Steel and Composite Structures
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• 제46권1호
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• pp.107-114
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• 2023

A new intelligent adaptive control scheme was proposed that combines observer disturbance-based adaptive control and fuzzy adaptive control for a composite structure with a mass-adjustable damper. The most important advantage is that the control structures do not need to know the uncertainty limits and the interference effect is eliminated. Three adjustable parameters in LMI are used to control the gain of the 2D fuzzy control. Binary performance indices with weighted matrices are constructed to separately evaluate validation and training performance using the revalidation learning function. Determining the appropriate weight matrix balances control and learning efficiency and prevents large gains in control. It is proved that the stability of the control system can be ensured by a linear matrix theory of equality based on Lyapunov's theory. Simulation results show that the multilevel simulation approach combines accuracy with high computational efficiency. The M-TMD system, by slightly reducing critical joint load amplitudes, can significantly improve the overall response of an uncontrolled structure.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권3호
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• pp.1702-1721
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• 2019

A workflow process (or business process) management system helps to define, execute, monitor and manage workflow models deployed on a workflow-supported enterprise, and the system is compartmentalized into a modeling subsystem and an enacting subsystem, in general. The modeling subsystem's functionality is to discover and analyze workflow models via a theoretical modeling methodology like ICN, to graphically define them via a graphical representation notation like BPMN, and to systematically deploy those graphically defined models onto the enacting subsystem by transforming into their textual models represented by a standardized workflow process definition language like XPDL. Before deploying those defined workflow models, it is very important to inspect its syntactical correctness as well as its structural properness to minimize the loss of effectiveness and the depreciation of efficiency in managing the corresponding workflow models. In this paper, we are particularly interested in verifying very large-scale and massively parallel workflow models, and so we need a sophisticated analyzer to automatically analyze those specialized and complex styles of workflow models. One of the sophisticated analyzers devised in this paper is able to analyze not only the structural complexity but also the data-sequence complexity, especially. The structural complexity is based upon combinational usages of those control-structure constructs such as subprocesses, exclusive-OR, parallel-AND and iterative-LOOP primitives with preserving matched pairing and proper nesting properties, whereas the data-sequence complexity is based upon combinational usages of those relevant data repositories such as data definition sequences and data use sequences. Through the devised and implemented analyzer in this paper, we are able eventually to achieve the systematic verifications of the syntactical correctness as well as the effective validation of the structural properness on those complicate and large-scale styles of workflow models. As an experimental study, we apply the implemented analyzer to an exemplary large-scale and massively parallel workflow process model, the Large Bank Transaction Workflow Process Model, and show the structural complexity analysis results via a series of operational screens captured from the implemented analyzer.

• 센서학회지
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• 제15권5호
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• pp.357-361
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• 2006

This paper reports a new technology to implement complex PDMS microchannels, which are simply constructed using three-dimensional photoresist structures as mold for PDMS replica process. The process utilizes LOR resist as a sacrificial layer to levitate the structural photoresist and multi-step exposure to control the thicknesses of photoresist structures. Various shapes of photoresist structures were successfully fabricated. Using the PDMS replica method, the three-dimensional photoresist structures are demonstrated to be applicable for implementing complex microchannels in PDMS. In addition, more complex multilevel microchannels are constructed by bonding two PDMS layers with just single PDMS alignment.

• 전산구조공학
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• 제9권4호
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• pp.141-154
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• 1996

드로네이 요소생성기법은 모델링 영역의 모양에 구애받지 않으면서 요소의 크기제어, 재편성, 국지요소생성 등에 있어서 탁월한 기능을 보여주고 있다. 그러나 생성되는 요소가 선형삼각형요소임으로해서 비압축성 또는 대변위거동의 근사나 복잡한 형상의 영역의 기하학적 근사에 한계를 갖고 있다. 이를 보완하기 위해 기제시된 드로네이 요소생성 알고리즘을 바탕으로한 6절점 삼각요소 생성알고리즘을 제시하여 본 기법의 완성도를 높이고 이를 성형문제에 적용해 보였다.

• 국제초고층학회논문집
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• 제7권1호
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• pp.17-32
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• 2018

This paper explores the function of a structural skin with an embossed surface applicable to use for tall building structures. The major diagrid system with a secondary embossed surface structure provides an enhanced perimeter structural system by increasing tube section areas and reduces aerodynamic loads by disorienting major organized structure of winds. A parametric study used to investigate an optimized configuration of the embossed structure revealed that the embossed structure has a structural advantage in stiffening the structure, reducing lateral drift to 90% compared to a non-embossed diagrid baseline model, and results of wind load analysis using computational fluid dynamics, demonstrated the proposed embossed system can reduce. The resulting undulating embossed skin geometry presents both opportunities for incorporating versatile interior environments as well as unique challenges for daylighting and thermal control of the envelope. Solar and thermal control requires multiple daylighting solutions to address each local façade surface condition in order to reduce energy loads and meet occupant comfort standards. These findings illustrate that although more complex in geometry, architects and engineers can produce tall buildings that have less impact on our environment by utilizing structural forms that reduce structural steel needed for stiffening, thus reducing embodied $CO^2$, while positively affecting indoor quality and energy performance, all possible while creating a unique urban iconography derived from the performance of building skin.

• 한국해양공학회지
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• 제10권1호
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• pp.108-118
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• 1996

This paper presents automatic reentry of a deepsea reser by adaptive control. Reentry is one of the major pro blems regarding a deepsea riser. In the reentry operation, the lower end of riser must be accurately positioned over the tarket point on the seabed. But the deepsea riser shows complex elastic response due to flexibility and nonlinearity of the riser dynamics and the required positioning accuracy is high. Moreover, elastic deformation must by controlled for securing structural integrity. In adaptive control, uncertainly known parameters like added mass and drag coefficient in the riser dynamics are identified and control forces at the floating body and the riser are calculated simultaneously. An Adaptive algorithm for MIMO linear discrete time system without requiring a persistent excitation is adopted in this study. The effectiveness of adaptive control logic is tested by numerical simulation and model experiment. The designed control system shows good overall performances, so that the present study can be applied to the control of the deepsea riser.

• 한국구조물진단유지관리공학회 논문집
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• 제1권2호
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• pp.87-94
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• 1997

This research aims to develop an algorithm of optimal transducer placement using Kinetic Energy of the structural system. The structural vibration response-based health monitoring is considered one of the best for the system which requires a long-term, continuous monitoring. In its experimental modal testing, however, it is difficult to decide on the measurement locations and their number, especially for complex structures, which have a major influence on the quality of the results. In order to minimize the number of sensing operations and optimize the transducer location while maximizing the accuracy of results, this paper discusses about an optimum transducer placement criterion suitable for the identification of structural damage. As a criterion algorithm, it proposes the Kinetic Energy Optimization Technique (EOT), and then addresses the numerical issues which are subsequently applicable to actual experiment where a bridge model is used. By using the experimental data, it compares the EOT with the EIM (Effective Independence Method) which is generally used to optimize the transducer placement for the damage identification and control purposes. The comparison conclusively shows that the EOT algorithm proposed in this paper is preferable when a structure is to be instrumented with fewer sensors.

• Geomechanics and Engineering
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• 제19권3호
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• pp.283-293
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• 2019

Evaluating the stability of the excavation face of the cross-river shield tunnel with good accuracy is considered as a nonlinear and multivariable complex issue. Understanding the stability evaluation method of the shield tunnel excavation face is vital to operate and control the shield machine during shield tunneling. Considering the instability mechanism of the excavation face of the cross-river shield and the characteristics of this engineering, seven evaluation indexes of the stability of the excavation face were selected, i.e., the over-span ratio, buried depth of the tunnel, groundwater condition, soil permeability, internal friction angle, soil cohesion and advancing speed. The weight of each evaluation index was obtained by using the analytic hierarchy process and the entropy weight method. The evaluation model of the cross-river shield construction excavation face stability is established based on the idea point method. The feasibility of the evaluation model was verified by the engineering application in a cross-river shield tunnel project in China. Results obtained via the evaluation model are in good agreement with the actual construction situation. The proposed evaluation method is demonstrated as a promising and innovative method for the stability evaluation and safety construction of the cross-river shield tunnel engineerings.