• 제목/요약/키워드: complementation

검색결과 297건 처리시간 0.025초

Modification vs. Complementation

  • Park, B. S.
    • 한국언어정보학회:학술대회논문집
    • /
    • 한국언어정보학회 1994년도 The Proceedings of the 1994 Kyoto Conference
    • /
    • pp.41-48
    • /
    • 1994
  • PDF

Analysis of the Dimerization of Human CD99 Using Bimolecular Fluorescence Complementation Technique

  • Lee, Mi-Kyung;Kim, Hyun-Soo;Kim, Seung-Seok;Cho, Myung-Hwan;Lee, Im-Soon
    • Journal of Microbiology and Biotechnology
    • /
    • 제18권3호
    • /
    • pp.472-476
    • /
    • 2008
  • Two isoforms of human CD99 have been identified, but only heterotypic interaction between the isomers was recently demonstrated. In this study, we performed bimolecular fluorescence complementation analysis to further characterize the interaction in vivo. Upon transiently transfecting plasmids expressing either of the two isoforms fused with yellow fluorescent protein (YFP) fragments, all the YFP-tagged CD99 molecules were properly localized on cell surfaces, and formed fluorescent dimers. Interestingly, however, unlike the previous report, the homodimers formed as efficiently as the heterodimer via their extracellular domains, implying its distinct regulatory role through modulating the complex profile.

Cloning and Sequencing of the ddh Gene involved in the Novel Pathway of Lysine Biosynthesis from Brevibacterium lactofermentum

  • Kim, Ok-Mi;Kim, Hyun-Jeong;Kim, Dal-Sang;Park, Dong-Chul;Lee, Kap-Rang
    • Journal of Microbiology and Biotechnology
    • /
    • 제5권5호
    • /
    • pp.250-256
    • /
    • 1995
  • The ddh gene encoding meso-diaminopimelate (meso-DAP)-dehydrogenase (DDH) in Brevibacterium lactofermentum was isolated by complementation of the Escherichia coli dapD mutation. It was supposed from subcloning experiments and complementation tests that the evidence for DDH activity appeared in about 2.5 kb Xhol fragmented genome. The 2.5 kb Xhol fragment containing the ddh gene was sequenced, and an open reading frame of 960 bp encoding a polypeptide comprising 320 amino acids was found. Computer analysis indicated that the deduced amino acid of the B. lactofermentum ddh gene showed a high homology with that of the Corynebacterium glutamicum ddh gene.

  • PDF

친환경건축물 인증제도 평가 항목 보완에 관한 기초 연구 -오픈하우징 관련 항목을 중심으로- (The Basic Study on the Complementation of Green Building Certification Criteria in Consideration of Open Housing -Focus on the Related Items of the Open Housing-)

  • 황은경;임석호;이상호
    • 한국주거학회논문집
    • /
    • 제14권5호
    • /
    • pp.141-151
    • /
    • 2003
  • Recently, the housing market in Korea has been changed from new construction to building-stocks, and focusing on supplier to focusing on users. Open housing is a design method not only content to users' various needs but also to the means of sustainable development by adapted to longevity of buildings. There are some indicators for Open Housing related to Green Building Certification Criteria which were worked by Mock & ME. But those indicators are limited to flexible design methods. This is a basic study for the selecting of Open Housing indicators for complementation of Green Building Certification Criteria by the analysis of SI housing in Japan, domestic Green Building Labelling and those of other countries.

Genetic studies of Baculovirus used as a microbial pesticide

  • Lee, Hyung-Hoan
    • 한국미생물생명공학회:학술대회논문집
    • /
    • 한국미생물생명공학회 1979년도 추계학술대회 심포지움
    • /
    • pp.248.1-248
    • /
    • 1979
  • Sixteen temperature-sensitive mutants of Autographa californica nuclear polyhedrosis virus were isolated. Several interesting phenotypes were observed. A large proportion of the mutants were un-able to form polyhedral occlusion bodies at the nonpermissive temperature (32.5C). At 32.5C, one mutant formed plaques in which the cells lacked polyhedra. Another mutant type was defective in the production of progeny extracellular nonoccluded virus and produced a plaque consisting of only a single cell containing polyhedra at 32.5C. One mutant was defective in plaque formation, progeny nonocluded virus formation, and polyhedra formation at 32.5C. Several mutants produced nonocluded virus but failed to produce plaques or polyhedra at 32.5C. Other phenotypes were also distinguished. Complementation analyses, performed by either measuring the increase in extracellular non-ocludedvirus formation or by oberving polyhedra formation in mixed infections at 32.5C, indicated the presence of 15 complementation groups. A high frequency of recombination was observed. Four of the mutants were found to be host dependent in their temperature sensitivity for polyhedra formation.

  • PDF

Effects of R100 Mutant MerR on Regulation of mer Operon from Shigella flexneri

  • Yoon, Kyung-Pyo
    • Journal of Microbiology and Biotechnology
    • /
    • 제4권4호
    • /
    • pp.245-249
    • /
    • 1994
  • An amino-terminal 14 amino acids deletion and three site-directed mutations were created to investigate the mechanism of induction and repression of MerR regulatory protein in R100 mer operon from gramnegative Shigella flexneri. The amino-terminal 14 amino acids deletion, Cysl17Ser, and Cys126Ser mutations abolished the inducibility of the mer operon and the Hisl18Ala mutation resulted in the reduction of inducibility (about 9.1 % remaining) in complementation experiment in the presence of $Hg^{2+}$ at subtoxic level ($1\mu M$). The complementation experiment with $Hg^{2+}$ absent showed that Hisl18Ala, Cys126Ser, and wild-type MerR could repress the operon but Cysl17Ser could not, and the amino-terminal deletion mutant could neither induce nor repress the R100 mer operon.

  • PDF

Brevibacterium lactofermentum의 dapD 유전자의 Cloning 및 E. coli에서의 발현 (Cloning and Expression of the dapD Gene from Brevibacterium lactofermentum in E. coli)

  • 김옥미;박선희;박혜경;이승언;하대중;이갑랑
    • 한국식품영양과학회지
    • /
    • 제30권5호
    • /
    • pp.802-805
    • /
    • 2001
  • 산업적으로 lysine 발효 산업에 이용되고 있는 B. lactofermentum으로부터 lysine 생합성에 관여하는 tetrahyrodipicolinate N-succinyl transferase를 지령하는 dapD 유전자를 E. coli의 dapD 결손변이주와의 complementation test를 통하여 cloning하였다. 재조합 plamid는 3.6 kb의 DNA 단편을 함유하고 있었으며 Southern blot hybridization을 통하여 dapD 유전자는 B. lactofermentum으로부터 유래하였으며 염색체 DNA내에 single copy로 존재함을 알 수 있었다. 또한 lysine 생성량 분석을 통하여 E. coli에서 B. lactofermentum dapD 유전자의 발현을 확인하였다.

  • PDF

Virus-resistant and susceptible transgenic Nicotiana benthamiana plants expressing coat protein gene of Zochini green mottle mosaic virus for LMO safety assessment

  • Park, M.H.;B.E. Min;K.H. Ryu
    • 한국식물병리학회:학술대회논문집
    • /
    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
    • /
    • pp.146.1-146
    • /
    • 2003
  • Transgenic Nicotiana benthmiana plants harboring and expressing coat protein (CP) gene of Zucchini green mottle mosaic virus (ZGMMV) were generated for both virus-resistant screening and complementation analysis of related viruses and environmental safety assessment (SA) of living modified organism (LMO) purposes. Transformation of leaf disc of N. benthamiana was performed using Agrobacterium-mediated method and the pZGCPPGA748 containing the ZGMMV CP and NPTII genes. Two kinds of transgenic homozygous groups, virus-resistant and -susceptible lines, were obtained by screening of challenging homologous virus for T1 generations. Complementation of CP-deficient related virus was analyzed using the susceptible line of ZGMMV. These two pathologically different lines can be useful for host-virus interactions and LMO environmental SA.

  • PDF