• Journal of Genetic Medicine
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• 제20권2호
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• pp.39-45
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• 2023

As advanced sequencing technologies continue to uncover an increasing number of variants in genes associated with human genetic diseases, there is a growing demand for systematic approaches to assess the impact of these variants on human development, health, and disease. While in silico analyses have provided valuable insights, it is essential to complement these findings with model organism studies to determine the functional consequences of genetic variants in vivo. Drosophila melanogaster is an excellent genetic model for such functional studies due to its efficient genetic technologies, high gene conservation with humans, accessibility to mutant fly resources, short life cycles, and cost-effectiveness. The traditional GAL4-UAS system, allowing precise control of gene expression through binary regulation, is frequently employed to assess the effects of monoallelic variants. Recombinase medicated cassette exchange or CRISPR-Cas9-mediated GAL4 insertion within coding introns or substitution of gene body with Kozak-Gal4 result in the loss-of-function of the target gene. This GAL4 insertion strategy also enables the expression of reference complementary DNA (cDNA) or cDNA carrying genetic variants under the control of endogenous regulatory cis elements. Furthermore, the CRISPR-Cas9-directed tissue-specific knockout and cDNA rescue system provides the flexibility to investigate candidate variants in a tissue-specific and/or developmental-timing dependent manner. In this review, we will delve into the diverse genetic techniques available in Drosophila and their applications in diagnosing and studying numerous undiagnosed diseases over the past decade.

• 전기전자학회논문지
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• 제26권4호
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• pp.736-741
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• 2022

격자 기반 암호화는 최악의 경우를 기반으로 한 강력한 보안, 비교적 효율적인 구현 및 단순성을 누리기 때문에 포스트 양자 암호화 방식 중 가장 실용적인 방식이다. 오류가 있는 링 학습(R-LWE)은 격자 기반 암호화(LBC)의 공개키암호화(Public Key Encryption: PKE) 방식이며, R-LWE의 가장 중요한 연산은 링의 모듈러 다항식 곱셈이다. 본 논문은 R-LWE 암호 시스템의 중간 보안 수준의 매개 변수 집합을 대상으로 하여 근사 컴퓨팅(Approximate Computing: AC) 기술을 기반으로 한 모듈러 곱셈기를 최적화하는 방법을 제안한다. 먼저 복잡한 로직을 간단하게 구현하는 방법으로 LUT을 사용하여 근사 곱셈 연산 중 일부의 연산 과정을 생략하고, 2의 보수 방법을 활용하여 입력 데이터의 값을 이진수로 변환 시 값이 1인 비트의 개수를 최소화하여 필요한 덧셈기의 개수를 절감하는 총 두 가지 방법을 제안한다. 제안된 LUT 기반의 모듈식 곱셈기는 기존 R-LWE 모듈식 곱셈기 대비 속도와 면적 모두 9%까지 줄어들었고, 2의 보수 방법을 적용한 모듈식 곱셈기는 면적을 40%까지 줄이고 속도는 2% 향상되는 것으로 나타났다. 마지막으로 이 두 방법을 모두 적용한 최적화된 모듈식 곱셈기의 면적은 기존대비 43%까지 감소하고 속도는 10%까지 감소하는 것으로 나타났다.

• 문화기술의 융합
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• 제9권5호
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• pp.83-90
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• 2023

최근 젊은 세대의 전통 복식 체험이나, 퓨전 한복의 일상복화 등의 추세에 따라 한복용 헤어 액세서리의 개발이 필요한 실정이다. 본 논문의 연구 목적은 왕실 여성의 머리 장식을 활용하여 한복과 어울리는 실용적이고 현대적인 헤어 액세서리 디자인을 개발하여 한복 문화를 활용한 패션 콘텐츠 개발의 영역을 확장시키고, 전통 문화의 다양한 경험에 대한 수요를 충족시키는 것이다. 연구 방법은 문헌연구를 통해서 전통 머리모양 및 영왕비의 전통 장신구들을 조사하였고, 실증연구로 실물을 제작하였다. 제작과정은 먼저 전통 머리모양을 응용하여 나일론 메쉬로 기본 형태를 만들었고, 그 위에 장엄하고 화려한 왕실 유물을 활용한 디지털 텍스타일 프린팅 원단을 접목하여 궁극적으로 전통 장신구를 착용한 듯한 트롱프뢰유 기법을 줄 수 있도록 디자인을 설계하였다. 결과적으로 총 6개의 헤어 액세서리 디자인이 완성되었으며, 헤어 밴드, 헤어 핀, 헤어 고무밴드의 구조로 제작하였다. 또한 착용자의 헤어 스타일에 관계 없이 간편하게 착용할 수 있도록 하였고 나일론 메쉬 소재의 특유의 빳빳하면서 유연한 재질을 이용하여 볼륨감 있는 머리 모양을 효과적으로 표현하였는데, 이는 마치 머리카락과도 같은 시각적인 착시 효과를 즐길 수 있다는 장점이 있다. 이러한 연구 결과를 통해 일상의 유희와 희소성 있는 가치를 추구하는 대중들에게도 독특한 미적, 문화적 경험을 제시할 수 있을 것으로 생각된다.

• Journal of Animal Science and Technology
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• 제51권2호
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• pp.97-104
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• 2009

교류와 경쟁력 향상을 위해서는 검정기준, 평가방법과 모델 등에 많은 보완이 필요하다. 따라서, 본 연구는 현재 국내의 50단계의 심사점수를 낙농선진국에서 채택하고 있는 9점제로의 변환을 통해 국제기준을 국내 현실에 맞게 적용하기 위해 실시했다. 분석에는 한국 종축개량협회(KAIA)의 젖소에 대한 혈통기록 및 2001에서 2006년도 사이에 실시된 체형 측정 기록 중 최종점수와 15개 체형형질에 대해 초산차의 기록을 갖는 32,487두의 암소집단의 선형심사기록 자료가 이용되었다. 9점제로 변경한 집단이 더욱 정규성을 띄는 분포 특성을 나타내었다. 상관분석결과에서도 모든 형질에서 50점제와 9점제 심사 점수간에 0.98 이상의 상관계수를 나타내었다. 따라서, 50 점제에서 9점제로의 변경이 가능할 것으로 판단된다. 하지만, 9점제로의 변환 후 모든 형질에서 심사자에 따라 고도의 유의성을 보였고(P<0.001), 점수제간 F값은 발굽기울기와 앞유방의 붙음성은 심사자간 변이가 비교적 높게 나타났다. 이것은 9점제로의 변환 후에도 심사자의 주관적인 요소가 점수에 영향을 미치는 것을 의미한다. 따라서, 선형형질 점수제 변환은 환경효과에 대한 다양한 분석을 통하여 적합성 여부를 결정해야 할 것이다.

• 안보군사학연구
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• 통권13호
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• pp.1-41
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• 2016

US 8th Army in the attack on the Western Front was a situation that was frustrated by the 13th Corps Communist army. To this time support this, goals that have been granted to the US 1st Marine Division that initiated the attack in Mupyonri direction, lack the potential to achieve was not decisive. It was a operation specific erroneous judgment that occurred because there was no one accurately grasp the battlefield of the situation the wrong UN Forces Command. Tactical victory can be to maximize the operation outcomes. However, there is no possible failure of the operation is to expand the tactical success. This is because the failure of the operation, because directly linked to the success or failure of the war. Tactical victory can be to maximize the operation outcomes. However, operation specific failure is not it possible to expand the tactical victory. Therefore tactical success of the US 1st Marine Division, can not compensate for operation specific failure of the United Nations Forces Command. However, Chinese Communist Army 9th Corps is obsessed only victory of tactics to annihilate the US 1st Marine Division, by being fixation to the Chosin whole area, it was not possible to run a operational operation. Therefore tactical success df the US 1st Marine Division, Chinese Communist Army 9th Corps is to extinguish the ability to increase the number of the 13th Corps of the Western Front, 8th Army US have contributed to have escaped the crisis. In addition, the US 10th Corps while maintaining the combat force, by an important role to withdraw through the sea, was able to complement the misjudgment of the operation.

• Asian-Australasian Journal of Animal Sciences
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• 제28권4호
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• pp.485-493
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• 2015

This study evaluated the in situ ruminal degradability, and subsequent small intestinal digestibility (SID) of dry matter, crude protein (CP), and amino acids (AA) of cottonseed meal (CSM), sunflower seed meal (SFSM) and distillers dried grains with solubles (DDGS) by using the modified three-step in vitro procedure. The ruminal degradability and subsequent SID of AA in rumen-undegradable protein (RUP-AA) varied among three protein supplements. The result show that the effective degradability of DM for SFSM, CSM, and DDGS was 60.8%, 56.4%, and 41.0% and their ruminal fermentable organic matter was 60.0%, 55.9%, and 39.9%, respectively. The ruminal degradable protein (RDP) content in CP for SFSM, CSM, and DDGS was 68.3%, 39.0%, and 32.9%, respectively, at the ruminal solid passage rate of 1.84%/h. The SFSM is a good source of RDP for rumen micro-organisms; however, the SID of RUP of SFSM was lower. The DDGS and CSM are good sources of RUP for lambs to digest in the small intestine to complement ruminal microbial AA of growing lambs. Individual RUP-AA from each protein source was selectively removed by the rumen microorganisms, especially for Trp, Arg, His, and Lys (p<0.01). The SID of individual RUP-AA was different within specific RUP origin (p<0.01). Limiting amino acid was Leu for RUP of CSM and Lys for both RUP of SFSM and DDGS, respectively. Therefore, different protein supplements with specific limitations should be selected and combined carefully in growing lambs ration to optimize AA balance.

• 사상체질의학회지
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• 제15권1호
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• pp.39-49
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• 2003

1. The purpose of study The problem lies in the Sasang-constitutional-medicine is a subjectiveness of diagnosis that can be varied by every donor. Questionnare for the Sasang Constitution Classification II(QSCCII) was invented to solve this problem. This study was conducted for two purpose. The one is re-value the QSCCII. The other is to make a basis to upgrade QSCCII. 2. The method of study QSCCII was administered to 537 patients at Kyung Hee Oriental Medicine Hospital. Data was collected during 19 months from september 2000 to march 2002. For the purpose of this study, the collected data was analyzed by crosstabs, variation analysis and discrimination analysis. The analyzing program was SPSS 8.0 for Windows. And using this program I made the judgmental equation to re-value the QSCCII. 3. The result of study 1) not applied intensifying value The diagnostic discrimination abilities of the QSCCII is 61.5% about Taeyangin, 57.7% about Soyangin, 67.2% about Taeumin, 68.8% about Soemin. And its average is 65.0%. 2) applied intensifying value The diagnostic discrimination abilities of the QSCCII is 76.9% about Taeyangin, 59.9% about Soyangin, 68.9% about Taeumin, 71.2% about Soemin. And its average is 67.3%. 3) I propose a new diagnostic possibility with a different discriminant analysis and increase 42.3% of diagnostic discrimination ability considering 25% of accident diagnostic discrimination ability. 4) Development and complement of Taeyangin and Soyangin questions is needed to increase the accuracy of diagnosis.

• Childhood Kidney Diseases
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• 제20권1호
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• pp.23-28
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• 2016

Purpose: We investigated whether serum levels of insulin growth factor-1 (IGF-1) and insulin growth factor binding protein-3 (IGFBP-3) are valuable in predicting clinical outcomes or are correlated with other laboratory findings in children with Henoch-$Sch{\ddot{o}}nlein$ purpura (HSP). Methods: We examined 27 children who were consecutively admitted to our hospital with HSP between January 2011 and February 2012. Blood tests (C-reactive protein, white blood cell count, platelet count, erythrocyte sedimentation rate, albumin, immunoglobulin A, complement C3, antineutrophil cytoplasmic antibody, IGF-1, IGFBP-3) and urine tests were performed upon admission. IGF-1 and IGFBP-3 were resampled in the recovery phase. Controls included 473 children whose IGF-1 and IGFBP-3 were sampled for evaluating their growth, at the outpatient department of pediatric endocrinology in our hospital. IGF-1 and IGFBP-3 were compared between the HSP children and controls, and between the acute and recovery phases in HSP children. The ability of these values to predict clinical outcomes including renal involvement was analyzed using bivariate logistic regression analysis (BLRA). Results: IGF-1 and IGFBP-3 were not different between the HSP children and controls ($148.7{\pm}117.6$ vs. $69.2{\pm}96.9$, P=0.290: $3465.9{\pm}1290.9$ vs. $3597.2{\pm}1,127.6$, P=0.560, respectively). There was no significant difference in IGF-1 or IGFBP-3 between acute and recovery phases. Based on the BLRA, no variable, including IGF-1 and IGFBP-3, could predict clinical outcomes including the presence of nephritis Conclusion: We concluded that IGF-1 and IGFBP-3 do not predict clinical outcomes of HSP, including renal involvement, in this study.

• 한국가스학회지
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• 제25권6호
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• pp.14-21
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• 2021

-162℃ 초저온 상태의 LNG를 저장하는 저장탱크의 내조는 균열과 같은 결함에 대한 구조 건전성 평가가 필요하다. 전통적인 파괴역학 관점에서는 응력확대계수 K, J-적분 그리고 CTOD를 이용한 단일 매개변수 평가가 주로 수행되어왔다. 그러나 실제 구조에서 발생되는 균열선단은 구조물의 크기, 시편형상 그리고 인장과 굽힘과 같은 하중의 형태에 따라 구속효과의 차이로 인한 영향이 발생하게 된다. 단일 매개변수 파괴역학을 보완하기 위해 다양한 시도가 있었고, 대표적으로 Q-응력법이 있다. 본 논문에서는 비선형 탄성영역의 균열선단 응력장 평가에 적합한 J적분에 Q응력을 유도하여 2 매개변수 접근법을 사용하고자 한다. SENB 시편의 균열비 0.1~0.7 그리고 광폭시편 균열비 0.2~0.6에 시편 균열선단의 응력을 J-Q 평가법을 이용하여 구속효과를 정량적으로 평가 하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제9권3호
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• pp.315-317
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• 1996

An investigation was carried out to find out the strains of foot-and-mouth disease (FMD) virus in 24 districts of Bangladesh. A total of 505 FMD virus samples were collected from June, 1989 to June, 1991 and tested by complement fixation test (CFT). Of these, 276 (54.7%) were found positive for different strains of FMD virus and the rest 45.3% were either negative or anticomplementary. Strains identified were O, C, Asia-1 and sub-strains $A_5$ and $A_{22}$. Strain O was found to be most prevalent(39.8%) followed by Asia-1 (5.7%), C (5.3%), $A_5$ (3.4%) and $A_{22}$ (0.4%). Prevalence of sub-strain $A_5$ was reported for the first time in Bangladesh. District-wise typing of FMD virus has been done which would be helpful for appropriate vaccination programme in different districts of Bangladesh for control of the malady.