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A Fluorescent Recombinase Aided Amplification Assay for Detection of Babesia microti

  • Lin, Hong;Zhao, Song;Ye, Yuying;Shao, Lei;Jiang, Nizhen;Yang, Kun
    • Parasites, Hosts and Diseases
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    • v.60 no.3
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    • pp.201-205
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    • 2022
  • Babesia microti is one of the most common causative agents of babesiosis. A sensitive and rapid detection is necessary for screening potentially infected individuals. In this study, B. microti cytochrome c oxidase subunit I (cox1) was selected as the target gene, multiple primers were designed, and optimized by a recombinase-aided amplification (RAA) assay. The optimal primers and probe were labeled with fluorescein. The sensitivity of fluorescent RAA (fRAA) was evaluated using gradient diluents of the cox1 recombinant plasmid and genomic DNA extracted from whole blood of B. microti infected mice. The specificity of fRAA was assessed by other transfusion transmitted parasites. The analytical sensitivity of the fRAA assay was 10 copies of recombinant plasmid per reaction and 10 fg/µl B. microti genomic DNA. No cross-reaction with any other blood-transmitted parasites was observed. Our results demonstrated that the fRAA assay would be rapid, sensitive, and specific for the detection of B. microti.

Numerical investigation of glass windows under near-field blast

  • Chiara Bedon;Damijan Markovic;Vasilis Karlos;Martin Larcher
    • Coupled systems mechanics
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    • v.12 no.2
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    • pp.167-181
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    • 2023
  • The determination of the blast protection level and the corresponding minimum load-bearing capacity for a laminated glass (LG) window is of crucial importance for safety and security design purposes. In this paper, the focus is given to the window response under near-field blast loading, i.e., where relatively small explosives would be activated close to the target, representative of attack scenarios using small commercial drones. In general, the assessment of the load-bearing capacity of a window is based on complex and expensive experiments, which can be conducted for a small number of configurations. On the other hand, nowadays, validated numerical simulations tools based on the Finite Element Method (FEM) are available to partially substitute the physical tests for the assessment of the performance of various LG systems, especially for the far-field blast loading. However, very little literature is available on the LG window performance under near-field blast loads, which differs from far-field situations in two points: i) the duration of the load is very short, since the blast wavelength tends to increase with the distance and ii) the load distribution is not uniform over the window surface, as opposed to the almost plane wave configuration for far-field configurations. Therefore, the current study focuses on the performance assessment and structural behaviour of LG windows under near-field blasts. Typical behavioural trends are investigated, by taking into account possible relevant damage mechanisms in the LG window components, while size effects for target LG windows are also addressed under a multitude of blast loading configurations.

A Study on Unfairness of Customers according to New Management Strategy at Polarization of Retail Business

  • Kim, Jong-Jin
    • The Journal of Economics, Marketing and Management
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    • v.5 no.3
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    • pp.12-20
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    • 2017
  • The study examined effects of psychological change of distribution environment upon commercial areas to investigate consumers' experience and theory and to suggest power of new management strategy for growth of retailers A The study investigated actual conditions of business transaction of hyper market by blind interview. In April, 2016, the author visited 6 manufacturers to do depth interview. The questionnaire between food manufacturers and hyper market investigated the Association of Food Industries in Korea, NH Nonghyup and large manufacturers in July 2012. Questionnaires of 25 companies were used after excluding questionnaire having poor and inadequate answers. The sales commission with large scaled distribution business decreased (0.3 ~ 0.7) to increase additional expenses such as number of salesmen, interior expenses and economic costs (0.7 ~ 40%). (source: Fair Trade Commission). Fair Trade Commission released types and notice of unfair trade of large scaled retail business based on monopoly regulation and fair trade (hereinafter called 'notice of large scaled retail) to prevent large scaled distribution business from doing unfair trade. The notice controled unfair trade at different position between large scaled distribution business and small vendors.

Design, Deployment and Implementation of Local Area Network (LAN) at BAEC Head Quarter

  • Osman Goni;Md. Abu Shameem
    • International Journal of Computer Science & Network Security
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    • v.24 no.4
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    • pp.141-146
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    • 2024
  • A local area network (LAN) is a computer network within a small geographical area such as a home, school, computer laboratory, office building or group of buildings. A LAN is composed of interconnected workstations and personal computers which are each capable of accessing and sharing data and devices, such as printers, scanners and data storage devices, anywhere on the LAN. LANs are characterized by higher communication and data transfer rates and the lack of any need for leased communication lines. Communication between remote parties can be achieved through a process called Networking, involving the connection of computers, media and networking devices. When we talk about networks, we need to keep in mind three concepts, distributed processing, network criteria and network structure. The purpose of this Network is to design a Local Area Network (LAN) for a BAEC (Bangladesh Atomic Energy Commission) Head Quarter and implement security measures to protect network resources and system services. To do so, we will deal with the physical and logical design of a LAN. The goal of this Network is to examine of the Local Area Network set up for a BAEC HQ and build a secure LAN system.

Review on Regulatory and Technical Standards of Radiation Protection for Lens of the Eye (수정체 방사선 방호에 관한 규제기준 및 기술기준 검토)

  • Si Young Kim;Seok-Ju Hwang;Jae Seong Kim;Jung-Kwon Son
    • Journal of Radiation Industry
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    • v.18 no.1
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    • pp.1-7
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    • 2024
  • The International Commission on Radiological Protection (ICRP) lowered the annual equivalent dose limit of lens of the eye for radiation workers from 150 to 20 mSv in April 2011. This trend of lowering the equivalent dose limit for radiation workers has been observed worldwide, including international organizations such as the International Atomic Energy Agency (IAEA), International Organization for Standardization (ISO) and the European Commission (EC). In 2016, the Nuclear Safety and Security Commission of South Korea published research results that included a proposal for lowering the equivalent dose limit of lens of the eye for radiation workers in line with the ICRP recommendation. However, as of now, South Korea's Nuclear Safety Act and related regulations still specify an annual equivalent dose limit of lens of the eye as 150 mSv for radiation workers. The IAEA and ISO have issued guidelines regarding radiation protection for lens of the eye and recommended a dose level for the lens of the eye at 5 or 6 mSv per year for periodic monitoring of the equivalent dose for the lens of the eye.

Employment Effects Evaluation of Naver Shopping in 2018 (2018년 네이버 쇼핑의 고용영향 평가)

  • KIM, Heung-Kyu;JUNG, Yeon-Sung
    • The Journal of Industrial Distribution & Business
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    • v.10 no.5
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    • pp.27-36
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    • 2019
  • Purpose - Naver has emerged as a new leader in the open market. While existing open markets such as Gmarket, 11th Street, and so on are suffering from profitability deterioration, Naver is attracting sellers based on low commission and powerful search engine. We would like to analyze the impact of Naver shopping on the national economy, especially on employment, in a situation where the market reaction to Naver's strength as a leader in online shopping is mixed. Research Design, Data, and Methodology - Through the demand inducing inter-industry analysis, we estimate the employment inducement effect by Naver shopping from its shopping transaction. In turn, through the supply inducing inter-industry analysis, we estimate the employment inducement effect by Naver shopping from its low commission and powerful search engine. For the purpose of inter-industry analysis, as of 2018, the most recently announced 2014 inter-industry table (extension table) from the Bank of Korea is used. Results - The results of this study are as follows. First, Naver Shopping is expected to generate 7.8 trillion won's trade in 2018, resulting in 244,225 of job inducement, and 158,598 of employment inducement. In addition, Naver Shopping is estimated to benefit KRW 213 billion to its sellers due to low commission and powerful search function, resulting in 8,667 of job inducement, and 5,655 of employment inducement. Second, in terms of job inducement and employment inducement due to Naver Shopping's trade, transportation, business support service, information and communication, broadcasting, restaurants and lodging were ranked. Third, in terms of job inducement and employment inducement due to Naver Shopping's low commission and powerful search function, restaurants and hospitality, f/b and cigarette manufacturing, construction, and transportation equipment manufacturing were ranked. Conclusions - The number of job inducement resulting from low commission and powerful search engine of Naver shopping in 2018 was 8,667 (3.7% of 244,225, which was caused by transaction in Naver shopping in 2018), and employment inducement was 5,655 (3.7% of 158,598, which was caused by transaction in Naver shopping in 2018), which can be considered as additional employment impacts of Naver Shopping compared to the other online shopping operators.