• 한국미생물·생명공학회지
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• 제51권1호
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• pp.10-17
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• 2023

Dairy products are extensively used as carriers of probiotic strains that have potential health benefits. Assessment of the viability of probiotic strains during manufacturing is important to ensure that products meet recommended levels. Hence, the method for accurately quantifying lactic acid bacteria (LAB) in probiotic or dairy products is required. The present study aims to examine the performance of de-Man Rogosa Sharpe (MRS), plate count agar with bromocresol purple (PCA with BCP), and glucose blood liver (BL) agars recommended in the Korea Food Code guidelines for counting LAB. Analysis of the performance of culture media containing 19 lactic acid bacterial species commonly encountered in probiotic and dairy products showed no statistically significant difference between 18 reference strains and three culture media (p > 0.01). Furthermore, the suitability of three culture media was verified for the quantitative assessment of LAB in 25 probiotic and dairy products. The number of LAB in three culture media was determined to be more than 10⁷ colony-forming unit (CFU)/ml for fermented milk products and 10⁸ CFU/ml for condensed fermented milk and probiotic products, indicating that they all satisfied the Korea Food Code guidelines. Moreover, there was no statistically significant difference in the amount of LAB counted in all three culture media, suggesting that they can be used to isolate or enumerate LAB in commercial products. Finally, three culture media will be useful for isolating and enumerating LAB from fermented foods as well as gut microflora.

• 한국언론정보학보
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• 제66권
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• pp.28-58
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• 2014

2010년 말의 종편 승인 결정에서 현재 시점에 이르기까지 한국 방송산업은 상당 수준의 구조적 변동을 겪고 있다. 그 가운데 가장 특징적인 모습 하나는 단단한 수직결합 체계를 통해 국내 방송영상산업에서 강력한 콘텐츠 지배력을 유지해오던 지상파 제작 단위로부터 핵심 인력이 대규모로 유출되고 있는 현상일 것이다. 이 연구는 이와 같은 제작자 엑서더스 현상의 배후로서, 상업적 거대 미디어기업을 통해 방송부문에서 시장논리를 확대하려는 목적의식적 제도설계의 존재를 지목하고, 이와 같은 외부 요인이 방송산업 내부의 다양한 요인들과 맞물려 어떠한 양상을 빚어내고 있는지를 분석한다. 그 결과, 지상파 잔류보다는 이탈이 개인적 사회적 보상 차원에서 더 다양하고 더 큰 긍정유인을 제공한다고 본 제작자들 나름의 합리적 선택을 매개로 현재와 같은 대규모 이탈이 발생되었음을 확인하게 된다. 그러나 일부 복합 미디어 기업을 통해 진행되고 있는 이러한 창의성 재배치 과정이, 장기적으로 제작자들에게 더 많은 기회와 가능성을 보장하는 방향으로 귀결될지에 대해서는 다양한 의문이 제기된다. 이에 이 연구는 현재의 제도적 설계에 내재한 문제점을 지적하고, 이를 개선하기 위한 노력을 촉구한다.

• Journal of Microbiology and Biotechnology
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• 제17권3호
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• pp.393-397
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• 2007

A Haematococcus pluvialis strain isolated from the ruins of Ephesus in Turkey was investigated as regards its adaptation to laboratory conditions and maximum growth rate. In the first stage of the experiment, the growth of H. pluvialis was compared in common culture media. Furthermore, in an effort to minimize the culture costs, the second stage of the experiment compared the growth rate in the culture medium selected in the first stage with that in commercial plant fertilizers. The results demonstrated that the maximum cell concentration of 0.90 g/l, corresponding to a growth rate of $0.150d^{-1}$, was found with an N-P-K 20:20:20 fertilizer under a light intensity of $75{\mu}mol$ photons $m^{-2}s{-1}$ on the $12^{th}$ day of cultivation.

• 식품기술
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• 제18권1호
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• pp.91-98
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• 2005

Aims: As a preliminary experiment on new sanitizer delivery tools, the efficacy of aerosolizedsanitizer on foodborne pathogens was investigated in larger model chamber system.Methods: Peroxyacetic acid and hydrogen peroxide were aerosolized in a model system againstartificially inoculated target microorganisms on laboratory media. Cultures of 4 different foodborne pathogens were inoculated and affixed onto 3 different heights (bottom, wall, and ceiling), and 3different orientations (face-down, vertical, and face-down) inside a commercial semi-trailer cabinet(14.6 x 2.6 x 2.8 m). Sanitizer was aerosolized into 2 m droplet size fog and treated for 1 h atambient temperature.Results: Populations of Bacillus cereus, Listeria innocua, Staphylococcus aureus, and Salmonellatyphimurium were reduced by an average of 3.09, 7.69, 6.93 and 8.18 log units per plate, respectively.Interestingly, L. innocua, Staph. aureus, and Salm. typhimurium showed statistically not different (P$\leq$ 0.05) reduction patterns relative to height and orientation that were never expected in a sprayingsystemConclusion and significance: Aerosolized sanitizers diffuse like gaseous sanitizers, so it has greatpotential for use in commercial applications.

• Mycobiology
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• 제37권2호
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• pp.77-81
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• 2009

This study was conducted to identify the Colletotrichum species causing anthracnose disease of strawberry in Balgladesh and to evaluate in vitro activity of commercial fungicides it. Based on morphological and cultural characteristics, all 22 isolates were identified as Colletotrichum gloeosporioides. They developed white or glittery colonies with grey to dark grey reverse colony colors and they produced cylindrical conidia. The efficacy of five commercial fungicides, Bavistin DF, Dithane M-45, Sulcox 50 WP, Corzim 50 WP and Rovral 50 WP, were tested against the fungus. Bavistin inhibited radial growth completely and was followed in efficacy by Dithane M-45. In Bavistin DF treated media, the fungus did not produce conidia. The percent inhibition of radial growth of the fungus was increased with the increasing concentrations of fungicide.

• Journal of Microbiology and Biotechnology
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• 제9권4호
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• pp.386-392
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• 1999

A large-scale culture of hepatitis A virus in human diploid MRC-5 cells was conducted. In a roller bottle culture, the virus was grown to a maximum titer in 3 weeks after infection. Over 95％ of the cell-associated virus was excreted after culturing the infected cells in suspension media without fetal bovine serum for 3 days. The cultured virus was inactivated with formalin, concentrated by ultrafiltration, and partially purified by ultracentrifugation in a non-ionic gradient medium of Renocal. Two separate peak fractions showing high anti-HAY ELISA titer were pooled and about 40％ of HAV antigen was recovered by this purification procedure. Of the partially purified vaccine, the protein pattern in SDS-PAGE and immunogenicity in mice were compared with a commercial HAV vaccine. In SDS-PAGE, the purified vaccine in this study and the commercial vaccine showed almost the same protein pattern. The seroconversion rate of the purified vaccine in mice was not different from that of the commercial vaccine. Therefore, we could prepare a good grade of HAV vaccine by a simple purification procedure although the purification itself was not completed.

• 한국작물학회지
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• 제48권2호
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• pp.113-119
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• 2003

Soybean sprout pathogenic bacteria were isolated from the large, deep containers of a commercial factory. Over a period of one year, 40 pathogenic-like bacteria were isolated among a total of 732 isolates. In addition to bacteria previously reported to be associated with rotting, such as Pseudomonas putida and Erwinia carotovora, several other genera were also identified: Acinetobacter spp., Chryseobacterium spp., Klebsiella sp., Pantoea agglomerans, Bacillus sp. Fatty acid methyl ester (FAME) analysis using the Microbial ID (MIDI) system, and 16s rRNA sequence analysis, yielded identical results, confirming the identities of these microorganisms. Several types of selective media were not good for identification and determination of population structure in commercial environments, as colony type was not specific to the genus. There was no dominant bacterium, and we were not able to find the main bacterium responsible for soybean spout rot. Even though we did not identify a major target for controlling rot or screening for resistant cultivars, the results of this study indicated that bacterial rot of soybean sprout is endemic. In addition, it emerged that factory epidemics in summer are not caused by the bacteria isolated in this study.

• 한국정밀공학회지
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• 제25권5호
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• pp.103-111
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• 2008

This study proposes a technique to estimate the material property of a paper by using an experimental methods and commercial CAE software. Under gravitation, if one side of the paper is attached to the ground, the opposite side of paper is largely deformed, and vibrates freely. Since the paper has an orthotropic characteristic due to its treatment, the deformations in two orthogonal directions of the dry paper are different. An experimental method to measure the static deformation of the paper introduces this phenomenon. And dynamic behavior, frequency of free vibration is measured. And then. virtual prototypes that can represent the static and dynamic behavior are modeled by using the commercial CAE software $RecurDyn^{MT}$/MTT3D, which has been widely used by the printer makers. While comparing the deformation and frequency from the experiment and simulation, a design optimization technique in the commercial CAE software of R-INOPL, $RecurDyn^{TM}$/AutoDesign is used to estimate the material property such as Young's modulus, shear modulus and density of the paper.

• 국토계획
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• 제54권1호
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• pp.108-117
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• 2019

The purpose of this study is to investigate whether the plan reflecting the place in cultural regeneration was implemented and whether it was a change of place in culture through culture analyze. Mullae-dong has changed its urban structure into a textile factory, a manufacturing industry, an ironworks+artistic complex, and the Mullae art factory has been organizing a festival that has been created by artists and residents alike in the Mullae creative village. However, prior to the creation of the Mullae art factory in the social element, self-sustaining programs of Mullae creative villages were derived, but now commercial space is mainly derived. Therefore, it is necessary to plan for coexistence of ironworks, cultural space and commercial space rather than expanding into commercial space. Geumcheon-gu Geumcheon-gu Geumcheon Art Factory has become an integrated complex where various industries coexist in industrial complex. Geumcheon Art Factory also holds an artist support program every year and actively works as an international creative exchange center in Seoul. However, programs that act as a mediator are not implemented since 2014, and the Open Studio stops once a year. Therefore, Geumcheon-gu needs to expand programs that can include local programs and programs that can be combined with local cultural media.

• 복식
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• 제50권7호
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• pp.165-179
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• 2000

This research centers on studying the function and characteristic of fashion illustration for advertisement and publication which is, among plenty of fashion illustration's functions, the commercial one. Here are some purposes of the research. First, it presents the function of fashion illustration. Second, under the contemporary circumstance of specialization and subdivision. It givers people the better understanding of fashion illustration by providing a variety of its classification bases other than the typical, simple grouping of style drawing and fashion illustration. Third, this research helps fashion illustration establish itself as commercial art by showing how fashion illustration is utilized in the field as a part of the fashion industry. The methods and contents of the research are described that the concept of fashion illustration, characteristic, classification bases and commercial function are presented and than the data of fashion illustration which hale been used as goods since 1990 in the nation are selected in order to analyze the media for the application of fashion illustration. its expression methods, factors and images. By doing so, advertising fashion illustration are much less than those of published fashion illustration and even most cases of advertising fashion illustration are related just to poster advertisement such as magazines, fashion events or public subscription. The published fashion illustration isn't also attracting the public since it focuses on fashion information books, fashion magazines or fashion -clothing books. Another finding is that the expression style of fashion illustration is not various or distinctive. The expression remains in the trends of painting, figurative drawing, sometimes adopts post-modernism which is reflected in popular cartoon and figures exaggerates or simplifies the human body. The image of fashion illustration is simple. casual or feminine in the rough. For the development of commercial fashion illustration, it is required to produce fashion illustrators, not works but goody, with various concepts appealing to the public.