• Journal of Microbiology and Biotechnology
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• 제21권12호
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• pp.1299-1305
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• 2011

An important modification of thrombolytic agents is resistance to plasminogen activator inhibitor-1 (PAI-1). In previous studies, a new truncated PAI-1-resistant variant was developed based on deletion of the first three domains in t-PA and the substitution of KHRR 128-131 amino acids with AAAA in the truncated t-PA. The novel variant expressed in a static culture system of Chinese Hamster Ovary (CHO) DG44 cells exhibited a higher resistance to PAI-1 when compared with the full-length commercial drug; Actylase. In the present study, the truncated-mutant protein was expressed in CHO DG44 cells in 50 ml orbital shaking bioreactors. The final yield of the truncated-mutant in the culture was 752 IU/ml, representing a 63% increase compared with the static culture system. Therefore, these results suggest that using the combined features of a transient and stable expression system is feasible for the production of novel recombinant proteins in the quantities needed for preclinical studies.

• International Journal of Aeronautical and Space Sciences
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• 제18권2호
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• pp.279-289
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• 2017

In this study, a series of conjugate heat transfer (CHT) analyses are conducted for a stage of a fully cooled high-pressure turbine (HPT) at elevated levels of free stream turbulence (Tu = 5% and 25.7%). The goal of the analyses is to investigate the influence of high turbulence intensity on the fluid-thermal characteristics of a nozzle guide vane (NGV). The turbine inlet temperature is defined by considering a typical radial temperature distribution factor (RTDF). The Unsteady Reynolds Average Navier-Stokes (URANS) CHT simulations are carried out using CFX 15.0, a commercial CFD package. The presented CFD modeling approach for high turbulence intensity is verified with the experimental data from two types of NASA C3X NGVs with films. The computation grid is generated for both the fluid and solid domains. The fluid domain grid is created using a tetrahedral grid system with prism layers because of its complex geometry, and the solid domain grid is composed of only tetrahedral elements. The analytical results are compared to understand the effect of turbulence on flow characteristics and metal temperature distributions. The results obtained in this study provide useful insights on the effects of high free stream turbulence and unsteadiness. The results also lead to the proposal of meaningful turbine design guidelines.

• Spatial Information Research
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• 제20권6호
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• pp.99-107
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• 2012

최근 건설데이터를 활용하는 BIM과 공간데이터를 활용하는 GIS 간의 통합 및 연계를 시도하려는 연구가 많이 이루어지고 있다. 하지만, BIM과 GIS의 데이터모델이 서로 너무 다른 특징을 가지고 있어 두 데이터모델 간의 통합을 통한 상호운용은 매우 어려운 실정이다. 따라서 본 연구에서는 BIM과 GIS의 효율적인 상호운용성을 시스템적으로 확보하기 위한 BIM과 GIS 상호운용 플랫폼을 설계하여 제시한다. 그리고 기존의 상용 소프트웨어들을 분석하여 본 연구에서 제안한 플랫폼에 필요한 요소기술과 기능들을 정의하여 제시한다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2008년도 춘계학술대회논문집
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• pp.980-985
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• 2008

This paper presents vehicle road test of a semi-active suspension system equipped with continuously controllable magnetorheological (MR) dampers. As a first step, front and rear MR dampers are designed and manufactured based on the optimized damping force levels and mechanical dimensions required for a commercial middle-sized passenger vehicle. After experimentally evaluating dynamic characteristics of the MR dampers, the test vehicle is prepared for road test by integrating current suppliers, real-time data acquisition system and numerous sensors such as accelerometer and gyroscope. Subsequently, the manufactured four MR dampers (two for front parts and two for rear parts) are incorporated with the test vehicle and a skyhook control algorithm is formulated and realized in the data acquisition system. In order to emphasize practical aspect of the proposed MR suspension system, road tests are undertaken on proving grounds: bump and paved roads. The control responses are evaluated in both time and frequency domains by activating the MR dampers.

• 한국전산유체공학회지
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• 제5권1호
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• pp.1-7
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• 2000

In general, FDM(finite difference method) and FVM(finite volume method) are used for analyzing the fluid flow numerically. However it is difficult to apply them to problems involving complex geometries, multi-connected domains, and complex boundary conditions. On the contrary, FEM(finite element method) with coordinates transformation for the unstructured grid is effective for the complex geometries. Most of previous studies have used commercial codes such as KIVA or STAR-CD for the flow analyses in the engine cylinder, and these codes are mostly based on the FVM. In the present study, using the FEM for three-dimensional, unsteady, and incompressible Navier-Stokes equation, the velocity and pressure fields in the engine cylinder have been numerically analyzed. As a numerical algorithm, 4-step time-splitting method is used and ALE(arbitrary Lagrangian Eulerian) method is adopted for moving grids. In the Piston-Cylinder, the calculated results show good agreement in comparison with those by the FVM and the experimental results by the LDA.

• 한국소음진동공학회논문집
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• 제19권3호
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• pp.235-242
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• 2009

This paper presents vehicle road test of a semi-active suspension system equipped with continuously controllable magnetorheological(MR) dampers. As a first step, front and rear MR dampers are designed and manufactured based on the optimized damping force levels and mechanical dimensions required for a commercial middle-sized passenger vehicle. After experimentally evaluating dynamic characteristics of the MR dampers, the test vehicle is prepared for road test by integrating current suppliers, real-time data acquisition system and numerous sensors such as accelerometer and gyroscope. Subsequently, the manufactured four MR dampers(two for front parts and two for rear parts) are incorporated with the test vehicle and a skyhook control algorithm is formulated and realized in the data acquisition system. In order to emphasize practical aspect of the proposed MR suspension system, road tests are undertaken on proving grounds: bump and paved roads. The control responses are evaluated in both time and frequency domains by activating the MR dampers.

• 한국소음진동공학회논문집
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• 제23권5호
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• pp.463-471
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• 2013

This paper presents design and control of electronic control suspension(ECS) equipped with controllable magnetorheological(MR) damper for passenger vehicle. In order to achieve this goal, a cylindrical type MR fluid damper that satisfies design specification of a middle-sized commercial passenger vehicle is proposed. After manufacturing the MR damper with design parameters, their field-dependent damping forces are experimentally evaluated and compared with those of a conventional damper. A quarter-vehicle MR ECS system consisting of sprung mass, spring, tire, controller and the MR damper is established in order to investigate the ride comfort performances. On the basis of the governing equation of motion of the suspension system, five control strategies(soft, hard, comfort, sport and optimal mode) are formulated. The proposed control strategies are then experimentally realized with the quarter-vehicle MR ECS system. Control performances such as vertical acceleration of the car body and tire deflection are evaluated in frequency domains on random road condition. In addition, performance comparison of WRMS(weighted root mean square) of the quarter-vehicle MR ECS system on random road are undertaken in order to investigate ride comfort characteristics.

• 한국화재소방학회논문지
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• 제21권3호
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• pp.104-112
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• 2007

교육, 훈련, 연구에 사용된 의료 시뮬레이션 교육으로서 마네킹 시뮬레이터(mannequin simulator)의 개발은 오랜 시간에 걸쳐 이루어지고 있으며, 심폐소생술, 심장 수술, 마취 임상 수기, 위기관리(crisis management)에 대한 효율성 평가로 이어졌다. 최근 한국의 여러 의과대학에서 시뮬레이션 교육을 도입하여 임상 수기 교육을 하고 있으며, 시뮬레이션 센터를 설립하고 있다. 선진국의 응급의학분야에서 응급구조사의 역할이 매우 중요하게 대두되고 있으며, 한국에서 응급구조사의 효율적인 교육과 역할 수행을 위해 마네킹 시뮬레이터를 이용한 교육이 매우 중요하다. 본 연구는 이러한 의학 분야 시뮬레이션이 의과대학 교육뿐 아니라 구조구급 분야에 종사하는 소방공무원, 간호사, 응급구조사 양성 교육 등 의료의 여러 영역에서 다양하게 이용되는 의료시뮬레이션 진화와 교육에 대해 소개하고자 한다.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제10권2호
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• pp.125-133
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• 2004

개발 방법론과 도메인의 특성을 반영하여 테스팅 하기 위해서는 프로세스 표준을 테일러링 해야 하며 테일러링 된 프로세스에 따라 테스트를 수행해야 한다. 그러나 상용화된 테스트 도구들은 테일러링 된 프로세스의 일부만을 지원하기 때문에 실제 테스팅 현장에서는 개발 환경에 적합한 테스트 도구를 구입하거나 개발해야 한다. 본 논문에서는 프로덕트 라인 개발 개념을 이용한 테스트 프로세스 수행 도구 개발 방안을 제안하고 이를 ‘테스트 프로세스 수행 도구(Test Process Execution Tool: Test PET)’로 구현한다. 표준에 명시된 테스트 활동의 공통점과 차이점을 추출하여 이를 컴포넌트로 명세 및 구현하고 개발 방법론과 도메인에 맞는 특성을 반영하여 특정 개발 방법론과 도메인에 맞는 테스트 수행 도구를 개발하는 단계를 제안한다. 제안한 방안으로 구현된 Test PET은 개발 방법론과 도메인, 어플리케이션에 맞는 테스트 프로세스를 생성 한 후 생성된 테스트 프로세스에 따라 테스트를 수행할 수 있는 도구이다.

• 한국IT서비스학회지
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• 제8권1호
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• pp.203-215
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• 2009

This paper illustrates the application of co-occurrence theory to generate lightweight ontologies semi-automatically. The proposed model includes three steps of a (Semi-) Automatic creation of Ontology; (they are conceptually named as) the Syntactic-based Ontology, the Semantic-based Ontology and the Ontology Refinement. Each of these three steps are designed to interactively work together, so as to generate Lightweight Ontologies. The Syntactic-based Ontology step includes generating Association words using co-occurrence in web documents. The Semantic-based Ontology step includes the Alignment large Association words with small Ontology, through the process of semantic relations by contextual terms. Finally, the Ontology Refinement step includes the domain expert to refine the lightweight Ontologies. We also conducted a case study to generate lightweight ontologies in specific domains(news domain). In this paper, we found two directions including (1) employment co-occurrence theory to generate Syntactic-based Ontology automatically and (2) Alignment large Association words with small Ontology to generate lightweight ontologies semi-automatically. So far as the design and the generation of big Ontology is concerned, the proposed research will offer useful implications to the researchers and practitioners so as to improve the research level to the commercial use.