• Korean Journal of Environmental Agriculture
• /
• v.10 no.2
• /
• pp.119-127
• /
• 1991

In order to reveal the mechanism of heavy metal behavior in soils relating to factors such as soil pH, organic matter, C.E.C. and soil minerals influencing the activities of heavy metals, Cd, Cu and Zn were applied to soil columns filled with 8 different soils with adjusted soil pH to several levels between 3.0 to 11.0 and the amounts of adsorption and desorption of these heavy metals were measured. 1. At the adsorption maxima of three heavy metals(Cd, Cu and Zn) soil pH appeared to be near 6.0 regardless of properties of the 8 soils, and adsorption gradually decreased above and below pH 6.0. This phenomenon was the same in both heavy metal solutions and mixed solutions, and the mixed solution, containing three heavy metals, revealed slightly higher amounts of Cu adsorption and Cd adsorption. 2. It was also found that the adsorption of Cu and Zn by soils was positively correlated with C.E.C. and the organic matter of soils, respectively. However, the pH values showing maxima of heavy metal adsorption were negatively correlated with organic matter content by contrast with the correlation between the maxima and the C.E.C. values in soils. 3. The adsorption of Cu by soils markedly increased more with $Ca(OH)_2$ application than with NaOH application for soil pH adjusment. This was probably because of Ca effects in Cu precipitation in soils, in addition to the effect of the simple soil pH itself on Cu adsorption 4. It was also revealed that adsorbed Cu was hardly desorbed by $N-NH_4OAC$ solution from the Daejeong soil series compared to the Jeonbug and Yechun soil series. This was because the Daejeong soil series consisted of large amounts of expanding type Vermiculite minerals and also was high in C.E.C. and soil organic matter.

• Korean Journal of Ecology and Environment
• /
• v.48 no.4
• /
• pp.238-252
• /
• 2015

The effect of weir construction (2009~2011) was investigated on algal bloom dynamics and surrounding conditions in the Youngsan River by analyzing the long-term (2001~2014) data provided by the Water Information System, Ministry of Environment. The data include chlorophyll a and water properties such as total suspended solids (TSS), ammonium ($NH_4{^+}$), nitrate ($NO_3{^-}$), orthophosphate ($PO{_4}^{3-}$), total nitrogen (TN), total phosphorus (TP) and DIN/DIP molar ratio collected from 12 stations along the channel of the river. Temporal variations were examined using data collected monthly from 2001~2014 and Box-Whisker plot was used to examine the difference in algal bloom dynamics between before (2006~2008) and after (2012~2014) the weir construction. Pearson's correlation analysis was also used to analyze the correlation of parameters. The results showed that TSS affecting water turbidity increased during the construction but decreased especially at the stations located in the upper and middle regions of the river after the construction. Ammonium concentrations increased whereas the concentrations of other nutrients decreased after the construction inducing an increase in N:P molar ratio. Chlorophyll a decreased suddenly during the construction but increased clearly after the construction at the stations where TSS decreased. This indicates that algal blooms can develop in the Youngsan River due to a decrease in turbidity that increases light penetration in water column although the concentrations of nutrients such as orthophosphate were reduced after the weir construction.

• The Korean Journal of Food And Nutrition
• /
• v.18 no.3
• /
• pp.207-218
• /
• 2005

In room temperature, Kimchi becomes acidified and a little decayed, scenting a bad smell, and It couldn't be well kept. But if it should be made into a pill, it could be preserved for a long time for marketing, with nutrition highly concentrated as well as with no scent. Therefore, making Kimchi into a pill needs drying. When dried Kimchi, lactic acid and fragrant ingredient will vanish along with volatilization. The cyclodextrin(CD) as a stabilizer shows that the protecting rate of volatility of lactic acid in Kimchi is higher before than that of after fermentation, and it is higher at the addition $2\%\;than\;of\;1\%$ in case of Kimchi with CD. But it doesn't give much effect on total sugar, reducing sugar, protein and amino acid. Evaporation rate of lactic acid is the least in freeze dry, and natural dry, heat dry come next, respectively. In heat dry, if dried at more than $60^{\circ}C$ for a long time, Kimchi exudes boiling and scorched scent, causing bitter taste. The result of HPLC with superose 12 column at 280nm and 210nm shows that place and amount of main peak is almost the same, but the distribution of other peaks are different, with the revelation of various peaks like peptide and amino acid. The Kimchi pill made by the addition of $1\%$ CD shows that concentration is eight times higher than general Kimchi, total sugar is $14.4\%$, reducing sugar is $8.8\%$, protein is $4.8\%$, amino acid is $2.4\%$, and other contents are $74.4\%$, acidity is 32.8, and pH is 3.5 each. The result of letting 20 people with obesity, 20 patients with constipation have 30 pills(total weight 30g) three times a day for 60 days reveals they lost $2.29\%$ in weight on the average, and 7 among 20 were all relieved in constipation, and 8 responded that they experienced its efficacy.

• Korean Journal of Weed Science
• /
• v.18 no.3
• /
• pp.225-236
• /
• 1998

KSC-13906 [Erythro N-{(4,6-dimethoxypyrimidin-2-yl)aminocarbonyl} -2-(2-fluoro-1-hydroxy-n-propyl) benzenesulfonamide, US Patent 5,461,025] was investigated how can control phytotoxicity fluctuation and what a good method apply to new rice herbicide. The growth inhibition was observed when the rice plants was transplanted at a shallow depth(0 - 1cm) and leaching was low(0 - 1cm/ day) from the paddy soil. KSC-13906 appeared to move readily down into the paddy soil with water by 3cm depth in the soil column(${\phi}$ 10cm) filled with loamy sand soil under 3cm/day of leaching condition. Artificial control releasing pattern, designed as treated with KSC-13906 of 9 or 18g ai/ha either at a once or daily treated dividing volume of 1/20, 1/25 and 1/30 of the total volume, increased the safety of KSC-13906 to direct seeded and transplanted rice. The safety of KSC-13906 was also enhanced when KSC-13906 was mixed with dymron. For example, the mixture of KSC-13906 and dymron effectively reduced injury of direct seeded rice plants at 18 and 500g ai/ha, respectively, treated 7 days after transplanting. However, combination of KSC-13906 and several herbicides didn't show any synergistic effetct on herbicidal activity and safening effect on rice. However, the combination of KSC-13906+dymron (9~12+250~500g ai/ha) or KSC-13906+mefenacet+dymron(9+250+250g ai/ha) controlled almost all weeds in paddy field without causing any injury to rice and thus the combination would successfully be used as an oneshot herbicide in rice culture.

• Journal of Life Science
• /
• v.27 no.2
• /
• pp.202-210
• /
• 2017

As an effort to find a potential biopreservative, we isolated bacterial strains producing bacteriocin from fermented foods. A strain was finally selected and characteristics of the bacteriocin were investigated. The selected strain was identified as Bacillus subtilis E9-1 based on the 16S rRNA gene analysis. The culture supernatant of B. subtilis E9-1 showed antimicrobial activity against Gram-positive bacteria. Subtilisin A, ${\alpha}$-chymotrypsin, trypsin and proteinase K inactivated the antimicrobial activity, which means its proteinaceous nature, a bacteriocin. The bacteriocin activity was fully retained at the pH range from 2.0 to 8.0 and stable at up to $100^{\circ}C$ for 60 min. Solvents such as ethanol, isopropanol and methanol had no effect on the antimicrobial activity at the concentration of 100% but acetone and acetonitrile reduced the activity at up to 100% concentration. Cell growth of four indicator strains was dramatically decreased in dose-dependent manner. Listeria monocytogenes was the most sensitive, but Enterococcus faecium was the most resistant. Bacillus cereus and Staphylococcus aureus showed the medium sensitivity. The bacteriocin showed its antimicrobial activity against B. cereus and L. monocytogenes via bactericidal action. The number of viable cells of L. monocytogenes started to reduce after addition of bacteriocin to the minced beef. The bacteriocin was purified through acetone concentration, gel filtration chromatography and RP-HPLC. The whole purification step led to a 6.82 fold increase in the specific activity and 6% yield of bacteriocin activity. The molecular weight of the purified bacteriocin was determined to be 3.3 kDa by MALDI-TOF/TOF mass spectrometry.

• Journal of Korean Society of Environmental Engineers
• /
• v.35 no.8
• /
• pp.554-563
• /
• 2013

The purpose of this study is to evaluate the possibility of nutrient release at up and downstream of Kangchun weir, upstream of Yuju and Ipo weir in Namhan River. For this survey, we measured basic characteristics of the sediments (water content, ignition loss, TOC, TP, SRP, TN, phosphorus fractionation) and conducted nutrients release experiments under both aerobic and anaerobic condition. The overlying water from the sediment-water column was analyzed for nutrients (i.e. TP, $PO_4$-P, TN, $NO_3$-N, $NH_3$-N) everyday for 18days. Result of soil texture experiment showed that sediments are Sand. SRP concentration before the release experiment was different with the value after the release experiment. According to this result, we can find that there were more activated release processes in anaerobic condition. $PO_4$-P increased from 1 to 8 days and remained at the maximum value (7~8 days) afterward. The rapidly increase of $PO_4$-P was observed from 1 to 2~3 days whereas the TP continuously increase from 1 to 18 days. The $PO_4$-P release rate calculated by up to 7~8 days data highly correlated with initial SRP concentration with $R^2$=0.8502. $NO_3$-N release rate appears constantly decreasing trend as -5.7~-3.08 $mg/m^2{\cdot}day$, otherwise the $NH_3$-N release rate, by-product of a organic matter decomposition using nitrate as electron acceptor, was 0.57~2.41 $mg/m^2{\cdot}day$. Substantial portion in TN can be induced by organic nitrogen which originated from the tributary passing through non-point pollutant source. Compared with other similar researches, phosphorus and nitrogen release rates obtained in this study can be considered as relatively low values. Since this study targeted the sediments accumulated by one time of flooding season, there are limitation to generalize theses results. Therefore, it is necessary to consistently monitor and investigate the accumulation of nutrients in the sediment for understanding the effect of weir construction on the overlying water quality.

• Food Science and Preservation
• /
• v.23 no.5
• /
• pp.746-752
• /
• 2016

This research was carried out to identify aerodynamic property as a function of moisture content for designing equipment such as for post-harvest management. Terminal velocity of two wheat varieties {Backjjung (B) and Jogyeong (J)} with selected sound, damaged kernel and foreign materials (Wheat stick, Wheat husks) were measured with a designed vertical wind column at different moisture contents from about 9 to 30% wet basis. The results showed that terminal velocity of wheat and foreign materials except of Jogyeong's husks (p<0.05) had a significant difference at p<0.001. With increasing moisture content, the aerodynamic property values of the kernels and foreign materials of the two wheat varieties increased linearly. In detail, terminal velocity of sound and damaged kernel increased from 5.46 to 7.13 m/sec (B) and 7.48 to 8.60 m/sec (J), damaged kernel from 5.91 to 7.00 m/sec (B) and 6.48 to 7.75 m/sec (J). For foreign materials the terminal velocity of wheat stick increased from 2.92 to 4.07 m/sec (B) and 3.74 to 5.22 m/sec (J) whereas that of husks from 1.07 to 1.85 m/sec (B) and 2.02 to 2.33 m/sec (J) each. For air separation of wheat and foreign materials, the air flow should be less than 5.22 m/sec due to the range (1.07~5.22 m/sec) of foreign materials in wheat.

• The Korean Journal of Mycology
• /
• v.40 no.4
• /
• pp.229-234
• /
• 2012

A key molecule in the pathogenesis of Alzheimer's disease (AD) is the ${\beta}$-amyloid peptide ($A{\beta}$) generated by ${\beta}$-secretase activity, an aspartic protease. This study was designed to evaluate inhibitory effect of the high-molecular weight water-soluble polysaccharides (Et-P) isolated and purified from Phellinus linteus fruiting body on ${\beta}$-secretase activity. The Et-P was purified from the hot water extract of Phellinus linteus fruiting body mainly by 75% ethanol precipitation and DEAE-Cellulose column chromatography. From the DEAE-Cellulose chromato-gram and molecular weight analysis, the Et-P was shown to be a mixture of three polysaccharides with molecular mass of 1,629, 1,294, and 21 kDa, respectively. The monosaccharide composition of Et-P was determined to be glu-cose, galactose, and mannose as major sugars, glucose being the most prominent one (48% in mole percentage). The elemental analysis and FT-IR analysis suggested that Et-P is typical polysaccharides having at least partially ${\beta}$-linkages and possible existing as complex with phenolic compounds. The laminarinase digestion and HPAEC-PAD analysis suggested that Et-P is a variant of beta-(1,3)-glucans. The Et-P showed DPPH radical scavenging activity and, especially, a significant inhibitory activity on ${\beta}$-secreatase activity (48% inhibitin at 100 ${\mu}g/mL$), suggesting that they may inhibit the formation of $A{\beta}$ which is the major causative of Alzheimer's disease. The results of this study suggest that the water soluble polysaccharides of Phellinus linteus fruiting body can be a potent material for the development of preventive or therapeutic agents for AD.

• Korean Journal of Environmental Biology
• /
• v.35 no.4
• /
• pp.694-705
• /
• 2017

The aim of this study was to isolate and identify marine bacterium with anti-methicillin-resistant Staphylococcus aureus (MRSA) activity, and to purify the anti-MRSA compound, as well as to determine its activity and synergistic effects. Among the marine bacteria isolated in this study, the YJ-1 isolate had the strongest anti-MRSA activity. The YJ-1 isolate was identified on the basis of its biochemical characteristics and an analysis of 16S rRNA gene sequences. The YJ-1 isolate showed over 99.2% homology with Pseudomonas stutzeri, and was designated as a Pseudomonas sp. YJ-1. The optimal culture conditions were $25^{\circ}C$ and initial pH 7.0. For the purification of the anti-MRSA compounds, the YJ-1 was cultured in Pa PES-II medium, and the culture filtrates were extracted by ethyl acetate, hexane, and 80% MeOH. The 80% MeOH fraction was separated by a $C_{18}$ ODS column, silica gel chromatography and a reverse phase HPLC, to yield three anti-MRSA agents, the MR1, MR2, and MR3 compounds. When the MR1 compound of $250{\mu}g\;mL^{-1}$ concentration was applied to the MRSA cells, over 95% of bacterial cells was killed within 48 hr. Compared with vancomycin and ampicillin, the MR1 compound showed significant anti-MRSA activity. In addition, the anti-MRSA activity was increased by dose and time dependent manners. Furthermore, the combination of an MR1 compound with vancomycin produced a more rapid decrease in the MRSA cells than did the MR1 compound alone. Taken together, our results suggest that the Pseudomonas sp. YJ-1 and its anti-MRSA compounds could be employed as a natural antibacterial agent in MRSA infections.

• Korean Journal of Remote Sensing
• /
• v.37 no.2
• /
• pp.275-290
• /
• 2021

Sulfur dioxide (SO2) in the atmosphere is mainly generated from anthropogenic emission sources. It forms ultra-fine particulate matter through chemical reaction and has harmful effect on both the environment and human health. In particular, ground-level SO2 concentrations are closely related to human activities. Satellite observations such as TROPOMI (TROPOspheric Monitoring Instrument)-derived column density data can provide spatially continuous monitoring of ground-level SO2 concentrations. This study aims to propose a 2-step residual corrected model to estimate ground-level SO2 concentrations through the synergistic use of satellite data and numerical model output. Random forest machine learning was adopted in the 2-step residual corrected model. The proposed model was evaluated through three cross-validations (i.e., random, spatial and temporal). The results showed that the model produced slopes of 1.14-1.25, R values of 0.55-0.65, and relative root-mean-square-error of 58-63%, which were improved by 10% for slopes and 3% for R and rRMSE when compared to the model without residual correction. The model performance by country was slightly reduced in Japan, often resulting in overestimation, where the sample size was small, and the concentration level was relatively low. The spatial and temporal distributions of SO2 produced by the model agreed with those of the in-situ measurements, especially over Yangtze River Delta in China and Seoul Metropolitan Area in South Korea, which are highly dependent on the characteristics of anthropogenic emission sources. The model proposed in this study can be used for long-term monitoring of ground-level SO2 concentrations on both the spatial and temporal domains.