• The Korea Journal of Herbology
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• v.25 no.2
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• pp.129-136
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• 2010

Objective : Sinapis alba L. (SA) is a korean traditional herbal medicine that is usually used to prevent or treat inflammatory diseases, such as respiratory infection and rheumatoid arthritis. However, the effects of SA supplementation in vitro on serum antibody levels, splenocyte and peritoneal macrophage immune responses have not yet been determined. In this study, we examined the effect of SA on the production of Th1/Th2 cytokines. Methods : Splenocytes were isolated from naive C57BL/6 mice. Cells were enriched for CD4+ cell populations by first staining the cells with anti-CD4 (BD PharMingen, Calif, USA). CD4+ T cells were selected on a (CS) column, and the flow-through was collected as CD4+ T cells. Isolated cells were activated by overnight incubation on 24-well plates coated with $1{\mu}g/mL$ anti-CD3, $1{\mu}g/mL$ anti-CD28 and with SA ($100{\mu}g/mL$). Primary macrophages were collected from the peritoneal cavities of mice (8-week-old female C57BL/6). The peritoneal macrophages were washed and plated with RPMI-1640 overnight for the experiments. After 48-hours cultures, samples were centrifuged at 2000 rpm for 10 minutes, and the supernatants were stored at $-80^{\circ}C$. Mouse IL-4, IFN-$\gamma$ and TNF-$\alpha$ were quantified using ELISA kits (BioSource International, Camarillo, Calif, USA) according to the manufacturer's protocols. Results : SA at 100ug/ml decreased the generation of Th1 cytokine (IFN-$\gamma$) by 0.5-fold. However, SA has no effect on Th2 (IL-4) production. Conclusions : These results suggest that SA may play an important role in the control of T-cell-mediated autoimmunity by down-regulation of Th1 cytokine (especially IFN-$\gamma$, TNF-$\alpha$). These data may contribute to the design of new immunomodulating treatments for a group of autoimmune diseases.

• Journal of the Korea Concrete Institute
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• v.20 no.2
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• pp.213-219
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• 2008

The purpose of this study is to propose frame analysis method for flat plate slabs having edge beam under lateral loads. Flat plate system is defined as the system only with slab of uniform thickness and column. However, the slab system generally incorporate edge beams at exterior connection in actual design. ACI 318 (2005) allows three methods for conducting flat plate system analysis subjected to lateral loads. There are the finite element method (FEM), the equivalent frame method (EFM), and the effective beam width method (EBWM). Among methods, the EBWM enables us to analyze practically by substituting the actual slab to beam element. In this model, the beam element has a thickness equal to that of the slab, and effective beam width equal to some fraction of the slab transverse width. However, the established EBWM was generally proposed for variables of geometry or stiffness reduction factor and seldom proposed for the effect of edge beams. This study verifies that, in the case of flat plate system having edge beams at exterior connections, the lateral stiffness is considerably larger than without edge beams. Therefore it need to analysis method for considered the effect of edge beams. In this study, an analysis model is proposed for the flat plate system having edge beams under lateral loads by considering the effect of edge beams. To verify the accuracy of proposed model, this study compared results of the proposed EBWM with results of FEM of flat plate systems having edge beams under lateral loads. Also, the proposed approach is compared with experimental results of former research.

• Restorative Dentistry and Endodontics
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• v.24 no.2
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• pp.330-336
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• 1999

In biological systems, the mineral that forms hard tissue is of an apatitic nature, and hydroxyapatite($Ca_5OH(PO_4)_3$: HA) is generally considered as the prototype for such a mineral. Thus, the precipitation of HA, having biological implications, has been the subject of several investigations. Crystal growth studies using HA seeds in supersaturated solutions have enhanced our understanding of the process and mechanism involved in seeded crystal growth. From these studies, it has become apparent that the precipitation rate of HA onto the seed crystals depends on the various conditions, especially on the additives. The relation between the supersaturated solution containing fluoride and the process of HA crystal growth enhances the understanding of mechanism of HA crystal growth. Until recently, the studies have been on the crystal growth of enamel minerals and synthetic HA seeds in the supersaturated solution containing 1~2 ppm fluoride. The purpose of the present investigation is to study the effect that fluoride of high concentration has on the crystal growth kinetics of HA. In order to produce the composition found in the secretory enamel fluid, experimental solutions of 1mM Ca, 3mM P, and 100mM Tris as background electrolyte were used. Then this experimental solutions were added to 0, 2, 4, 6, 8, 10 ppm fluoride. The effect of fluoride at high concentrations on the precipitation was examined in a bench-top crystal growth model adopting a miniaturized reaction column. Chemical analysis was employed for characterization of working solutions before and after the experimentation. Remarkable findings were : 1) the amount of crystal growth was gradually accelerated as the fluoride concentration increased until 6 ppm, but decreased in 8 and 10 ppm fluoride; 2) the amount of fluoride ion consumed in crystal formation was constant despite the increase in fluoride concentration.

• The Journal of Pediatrics of Korean Medicine
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• v.21 no.3
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• pp.109-124
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• 2007

Objectives In the present study, the author intended to investigate whether Gamitonggyu-tang (GTT) significantly affects (since the subject is GTT, you need an 's') in vivo and in vitro mucin secretion from airway epithelial cells. Methods In vivo experiment, mice's mucin which is on a hypersecretion of an airway, mice's tracheal goblet cells in hyperplasia and mice's intraepithelial mucosubstances were exposed with SO2 for 3 weeks. Effects of orally-administered GTT for 1 week on in vivo mucin secretion and hyperplasia of tracheal goblet cells were assessed by using enzyme-linked immunosorbent assay (ELISA) and staining goblet cells with alcian blue. In vitro experiment, confluent hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of GTT to figure out the effectiveness of 3H-mucin secretion. Total elution profiles of control spent media and treatment sample through Sepharose CL-4B column were analyzed.Possible cytotoxicities of each agent were assessed by measuring lactate dehydrogenase (LDH) release. Also, the effect of GTT on contractility of isolated tracheal smooth muscle was investigated. Results (1) GTT inhibited hypersecretion of in vivo mucin. However, it did not affect the increase the number of goblet cells (2) GTT significantly increased mucin release from cultured HTSE cells, without significant cytotoxicity (3) GTT chiefly affected the 'mucin' secretion and did not affect the secretion of the other releasable glycoproteins with less molecular weight than mucin (4) GTT did not affect Ach-induced contraction of isolated tracheal smooth muscle.Conclusions This result suggests that GTT can increase mucin secretion during short-term treatment (in vitro) whereas it can inihibit hypersecretion of mucin during long-term treatment (in vivo). The author suggests that the effect GTT with their components should be further investigated and it is valuable to find from oriental medical prescriptions, novel agents which might regulate mucin secretion from airway epithelial cells.

• Korean Journal of Soil Science and Fertilizer
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• v.19 no.3
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• pp.217-221
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• 1986

A column test was conducted to find out the effect of application of slaked lime, calcium sulfate, calcium superphosphate, and phosphoric acid on the solubility of lead in soil. The soil was adjusted to 310.8 ppm concentration of Pb and applied with amounts of calcium equivalent to 600, 1000, 2000 ppm as slaked lime; sulfate 144, 288, 432 ppm as calcium sulfate; phosphate 95, 190, 285 ppm as calcium superphosphate and phosphoric acid, respectively. The results obtained are as follows: 1. The increasing application of improvement agents reduced the amounts of water soluble Pb in soil. Phosphoric acid was the most effect among to the treatments. 2. The slaked lime treatment has the highest pH of soil and the lowest at the phosphoric acid one. The soil Eh has a reverse tendency the soil pH. 3. Water soluble Ca, $PO_4$ and $SO_4$ contents increased with increasing application amounts of improvement agents in soil. 4. $1N-NH_4$ OAC soluble Pb content in soil was a decreasing tendency in the order of calcium superphosphate, phosphoric acid, slaked lime, calcium sulfate and control after experiment.

• The Journal of the Korea Contents Association
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• v.21 no.9
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• pp.125-135
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• 2021

Due to the lack of national consensus on the importance and value of the character industry and the lack of recognition of value as a national strategic industry, the development of the character industry is experiencing great difficulties. The purpose of this study is to analyze the economic effects of character industry in China to help establish policies and strategies for the character industry in the future. To this end, this study utilized the China 2017 Industrial Association Table. The analysis results are as follows. China's character industry has a lower production inducement effect than other industries with a column total of 3.45514, and a row total of 1.30015. This shows that China's character industry is still being produced by small and medium-sized companies with a low equity ratio. Second, in the character industry, the index of the sensitivity of dispersion representing the forward linkage effect is 0.01426 and the impact factor is 0.03790, which are all less than 1. Therefore, it can be said to be the final demand manufacturing type.Third, in China character industry's income induction is 0.47690 and the production tax induction effect is -0.04912. It can be seen that the character industry has less income induction and tax burden generated every time the final demand increases by one unit in the entire industry than in other industries.Despite the quantitative growth of the character industry in China, the impact on other industries is low and it is not playing a role as an income-generating industry. Structural improvement is needed for the qualitative development of China's character industry.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.3
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• pp.213-218
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• 2021

In this study, Kombucha, a fermented drink by adding beneficial bacteria to green tea or black tea with sugar, was classified using column chromatography, and the presence or absence of quercetin glycoside, a type of flavonoids, was confirmed through TLC. In addition, the anti-pollution effect of protecting and improving the skin from pollution was confirmed using the fraction. Keratinocytes were treated with K-QG to confirm the cell viability, showing a survival rate of 90% or more up to a concentration of 100 ㎍/mL, and the cell viability by benzo[e]pyrene and fine dust stimulation to see the anti-pollution effect was measured. At 100 ㎍/mL, the improvement rates were about 68.79% and 50.68%, respectively. In addition, as a result of confirming the expression of AhR activated by benzo[a]pyrene through Western blot, it showed an inhibition rate of about 31.08% at 100 ㎍/mL compared to the control group. Through the results of this study, K-QG is thought to be valuable as an anti-pollution functional material, protecting and improving skin irritated from benzopyrene and fine dust.

• Journal of the Korean Geotechnical Society
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• v.38 no.4
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• pp.47-58
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• 2022

Many 1g shaking table tests with an SDOF structure supported by a single pile were performed to evaluate the soil-structure interaction (SSI) effect. Since most structures supported by group piles are MDOF structures with columns, the SSI effect is simulated using a large 1g shaking table test and numerical analysis. According to the results, the movement in the piles tends to increase with input acceleration and when the input frequency is similar to the natural frequency. Furthermore, the slope of the dynamic p-y curve remains constant regardless of the variation of acceleration and input frequency. According to the results of the dynamic p-y backbone curve and the moment of group piles, a center pile with a leading pile has more soil resistance than side piles with a trailing pile, and the effect of group piles is observed above the 7D center to center pile distance.

• Journal of Microbiology and Biotechnology
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• v.8 no.5
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• pp.455-460
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• 1998

In the course of screening for new antitumor substances, a novel cytotoxic agent inducing apoptotic cell death was isolated from the culture broth of marine bacterial strain SCRC A-20. Strain SCRC A-20 was separated from a mollusk and was chemotaxonomically identified as a Streptomyces sp. The cytotoxic substance was purified by organic solvent extraction followed by silica gel column chromatography and preparative TLC. HRFAB-MS determined its molecular formula to be $C_{30}H_{40}N_2O_5$ (MW 508). The 1D and 2D NMR spectral data demonstrated that the substance has a novel lactam structure of a 20-membered macrocycle coupled with a unique acyl tetramine and bicyclo［3.3.0］ octane, which includes three methyl groups, six olefinic protons, five carbonyl groups, a conjugated diene and a dienone. The substance, named aburatubolactam C, appeared to be cytotoxic for various continuously proliferating tumor cells of human and murine origins. The $IC_{50}$ values determined by MIT assay were in the range of 0.3 to $5.8\mug/ml$. When Jurkat T cells were treated with $3\mug/ml$. of aburatubolactam C, the apoptotic DNA fragmentation was detectable within 3 h, indicating that the cytotoxic effect of aburatubolactam C on tumor cells is attributable to the induced apoptosis.

• Journal of Korean Society of Steel Construction
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• v.20 no.5
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• pp.625-632
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• 2008

In the companion paper (Model Development), an analytical model estimating the available rotation capacity of fully restrained beam-column connections in special steel moment-resisting frames was proposed. In this paper, two limit states were considered as the connection rotation capacity criteria: (i) strength degradation failure when the strength falls below the nominal plastic strength due to the local buckling of the beam's cross-section and (ii) low-cycle fatigue fracture caused by plastic strain accumulation at the buckled flange after only a few cycles of high-amplitude deformation. A series of analyses are conducted using the proposed model with two limit states under monotonic and cyclic loadings. Beam section geometric parameters, such as flange and web slenderness ratios, varied over the practical ranges of H-shapedbeams to observe their effect on the rotation capacity and low-cycle fatigue life of pre-qualified WUF-W connections.